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The map label for this gene is iaaA [H]

Identifier: 121611498

GI number: 121611498

Start: 5081936

End: 5082898

Strand: Direct

Name: iaaA [H]

Synonym: Veis_4589

Alternate gene names: 121611498

Gene position: 5081936-5082898 (Clockwise)

Preceding gene: 121611497

Following gene: 121611499

Centisome position: 91.29

GC content: 72.38

Gene sequence:

>963_bases
ATGAAGAACGCCACGCCCGTCATCGCCATCCACGGCGGCGCCGGCACCATCAGCACCTCGACGACCAGCCCCGAGCAGGC
GCAGGCGTATCACGATGCGCTGCAGACGGTCGTGCGCGCCGCGCAGGCCGCGCTGCTCGAGGGCTTGTCGGCGCTCGACG
CCACCTGCCTCGCGGTCGAGATGCTCGAAGACTGCCCGCTCTTCAATGCCGGCTACGGCGCGGTGTTCACGCACGAAGAA
ACGCACGAGCTCGACGCGGCCGTGATGGACGGCGCCACGCTCGCGGCCGGCGCCATTGCCGGCGTGAGCCACGTGCGCCA
CCCGGTGCGCGCCGCGCGCGCCGTGCTCGAAGACGGCGCCCACGTGCTGCTGGCCGGCGCGGGCGCCGAGGCCTTCGCGC
GCGACCGCGGCCTGGAGATGGTCGAGCCCTTTTTCTTCTCGACCGACGCACGCCGCCAGCAGTTGTACCGCGTGCGCGAC
ACGGGCCGCGTGGTCACCGACCACGAAGGCGCCGCCATGACCAAGCCGCCGCTGGACGAAGACAAGAAGTTCGGCACCGT
CGGCGCCGTGGCGCTCGACATGCATGGCCACCTGGCCGCCGCCACCTCGACCGGCGGCATGACCAACAAGCGCGTCGGCC
GCGTCGGCGATTCACCGCTCATCGGCTCCGGCACCTACGCCGACGACCGCAGCGCCGCCATCTCGTGCACCGGCAGCGGC
GAAATGTTCATCCGCGTCGCGGCTGCGCACGACATCTGCGCGCGCATGGCCTACGGCGGCGCCACGCTCGAAGCGGCCAC
GCACGCCGTGGTGCACGGCTCGCTGCCCGCCATCGGCGGCCCCGGCGGGCTGATCGCCGTCGACCGCCACGGCAACCTGA
GCCTGGCCTTCAACACCGAAGGCATGTACCGCGGCCACGCGCGCGGCGACGCAGCGCCGGTGACGGCCATCTTCGCAGCA
TGA

Upstream 100 bases:

>100_bases
GGCTGGGCCAGGGCCATGCGCGCGGCCAGGTCTCCATGCCTGCCCTCCTTCCCGTTGCCGTCGCTTCCGACGCCGGCAAG
AAAAAGAAGAAAGCTTCCCG

Downstream 100 bases:

>100_bases
AGCACACCCCCAGTCTTCGCGCACTTCGTGTCGCTTCGCCACCCCCCTCGCCGGGGGCAGCACCAGCGGCCCGGCAAAGC
CGGTTCTGCGGTGTTCCGCG

Product: peptidase T2, asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKNATPVIAIHGGAGTISTSTTSPEQAQAYHDALQTVVRAAQAALLEGLSALDATCLAVEMLEDCPLFNAGYGAVFTHEE
THELDAAVMDGATLAAGAIAGVSHVRHPVRAARAVLEDGAHVLLAGAGAEAFARDRGLEMVEPFFFSTDARRQQLYRVRD
TGRVVTDHEGAAMTKPPLDEDKKFGTVGAVALDMHGHLAAATSTGGMTNKRVGRVGDSPLIGSGTYADDRSAAISCTGSG
EMFIRVAAAHDICARMAYGGATLEAATHAVVHGSLPAIGGPGGLIAVDRHGNLSLAFNTEGMYRGHARGDAAPVTAIFAA

Sequences:

>Translated_320_residues
MKNATPVIAIHGGAGTISTSTTSPEQAQAYHDALQTVVRAAQAALLEGLSALDATCLAVEMLEDCPLFNAGYGAVFTHEE
THELDAAVMDGATLAAGAIAGVSHVRHPVRAARAVLEDGAHVLLAGAGAEAFARDRGLEMVEPFFFSTDARRQQLYRVRD
TGRVVTDHEGAAMTKPPLDEDKKFGTVGAVALDMHGHLAAATSTGGMTNKRVGRVGDSPLIGSGTYADDRSAAISCTGSG
EMFIRVAAAHDICARMAYGGATLEAATHAVVHGSLPAIGGPGGLIAVDRHGNLSLAFNTEGMYRGHARGDAAPVTAIFAA
>Mature_320_residues
MKNATPVIAIHGGAGTISTSTTSPEQAQAYHDALQTVVRAAQAALLEGLSALDATCLAVEMLEDCPLFNAGYGAVFTHEE
THELDAAVMDGATLAAGAIAGVSHVRHPVRAARAVLEDGAHVLLAGAGAEAFARDRGLEMVEPFFFSTDARRQQLYRVRD
TGRVVTDHEGAAMTKPPLDEDKKFGTVGAVALDMHGHLAAATSTGGMTNKRVGRVGDSPLIGSGTYADDRSAAISCTGSG
EMFIRVAAAHDICARMAYGGATLEAATHAVVHGSLPAIGGPGGLIAVDRHGNLSLAFNTEGMYRGHARGDAAPVTAIFAA

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=303, Percent_Identity=38.2838283828383, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI145275200, Length=303, Percent_Identity=38.2838283828383, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI98991769, Length=267, Percent_Identity=31.4606741573034, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI285002251, Length=256, Percent_Identity=32.03125, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787050, Length=312, Percent_Identity=58.974358974359, Blast_Score=350, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI32564649, Length=264, Percent_Identity=26.1363636363636, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI18921183, Length=315, Percent_Identity=35.8730158730159, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=33.2089552238806, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652135, Length=277, Percent_Identity=31.7689530685921, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221330102, Length=277, Percent_Identity=31.7689530685921, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24657358, Length=251, Percent_Identity=29.8804780876494, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 32880; Mature: 32880

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNATPVIAIHGGAGTISTSTTSPEQAQAYHDALQTVVRAAQAALLEGLSALDATCLAVE
CCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLEDCPLFNAGYGAVFTHEETHELDAAVMDGATLAAGAIAGVSHVRHPVRAARAVLEDGA
HHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HVLLAGAGAEAFARDRGLEMVEPFFFSTDARRQQLYRVRDTGRVVTDHEGAAMTKPPLDE
EEEEEECCCHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCC
DKKFGTVGAVALDMHGHLAAATSTGGMTNKRVGRVGDSPLIGSGTYADDRSAAISCTGSG
CCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCC
EMFIRVAAAHDICARMAYGGATLEAATHAVVHGSLPAIGGPGGLIAVDRHGNLSLAFNTE
CEEEEEEHHHHHHHHHHCCCCHHHHHHHEEEECCCCCCCCCCCEEEEECCCCEEEEECCC
GMYRGHARGDAAPVTAIFAA
CCEECCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKNATPVIAIHGGAGTISTSTTSPEQAQAYHDALQTVVRAAQAALLEGLSALDATCLAVE
CCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLEDCPLFNAGYGAVFTHEETHELDAAVMDGATLAAGAIAGVSHVRHPVRAARAVLEDGA
HHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HVLLAGAGAEAFARDRGLEMVEPFFFSTDARRQQLYRVRDTGRVVTDHEGAAMTKPPLDE
EEEEEECCCHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCC
DKKFGTVGAVALDMHGHLAAATSTGGMTNKRVGRVGDSPLIGSGTYADDRSAAISCTGSG
CCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCC
EMFIRVAAAHDICARMAYGGATLEAATHAVVHGSLPAIGGPGGLIAVDRHGNLSLAFNTE
CEEEEEEHHHHHHHHHHCCCCHHHHHHHEEEECCCCCCCCCCCEEEEECCCCEEEEECCC
GMYRGHARGDAAPVTAIFAA
CCEECCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]