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The map label for this gene is murD [H]

Identifier: 121611477

GI number: 121611477

Start: 5057306

End: 5059222

Strand: Direct

Name: murD [H]

Synonym: Veis_4568

Alternate gene names: 121611477

Gene position: 5057306-5059222 (Clockwise)

Preceding gene: 121611476

Following gene: 121611478

Centisome position: 90.85

GC content: 73.97

Gene sequence:

>1917_bases
ATGAATCCGAACGCCCAGCCCACCCTGCCCGCCCGGCCTCTGGATGAGGCCGGGGCGCCCGTGCCCGAGGCCCCCCCCGG
GGCACCCCGCGTAAAACCCCCGGTCAATCCCCCGGTCAACCCCCCGGCCATCTCCGCCGCGCAGGCCGCTGCCGATTTCG
TCGCGCAGATCTTTGCCTCCGTGCCGGCCCCCGAGGAGGCTGCGCCGGCCATGCCGCTGGCCATCGATCCGGCCATCAAC
CCGGTCATCAACCCGGCCATGCCCCCGGCCGAGGTGCCCGGCGCGCTGGCGGCCGAGCCGCTGCGGCGGCCCGGCGCAGA
CCTCCGGCAGGCGCTGCAAGACCAGCGCATCCTGATCCTCGGCCTGGGGGCATCGGGCCTGGCGATGGCGCGCTGGTGCC
TGCGCTGCGGCGCGCAGGTCACCGTGGCCGATACCCGTGCCGCGCCCCCGCAACTGCCGGCGCTGCAACGCGAACTGCCC
CGGGCGCGCTTCGTGCCCGGCGCCTTCGATGCCGGCCTGGTCGAGGGGCAGAACCTGCACGCGGTGTACTGCTCGCCGGG
CCTGAGCCCGTCCGCCGTGGCCGCCGTGGCGCAGACATGCAGCGCCCATGGCATCGGCATGGGCGGCGAGTTGGACCTGT
ATGCGCTGGCGCTGGCCGGGCTGCGCGCCGCCGATGGCTACGCGCCGACCGTGCTGGCCATCACCGGCACCAACGGCAAG
ACCACCGTGACCGCGCTCACCGGCCAGTTGCTCCAGCGCGCCGGCCGCAGCGTGGCGCTGGCCGGCAATATCGGCCCGAC
CCTGCTCGACACGCTGGCCGGGCATATCGACGCCGGCACCCTGCCTGCGGTGTGGGTGCTGGAGCTATCGAGCTTTCAAC
TCGAGCGCAGCACCGGTTTCGAGCCGACGGCGGCAGCGCTGCTCAACATCAGCCAAGACCATCTGGACTGGCATGGCGAC
ATGGCCGCCTACGCGGCGGCCAAGGCGCGCATCTTCGGCCAGACCGCGCTGATGGTTCTGAACCGCCAGGACGCTGCGGT
GATGCAGGTGTCGGCGCCTTCTGCGCCTTCTGCGCCGGTAACGCCAGCGCTGCGGCCCCGGGCGGCCAAAGCACCGGCGC
CGCAGCCACGCGCGGACATCACTTTCGGCGCCGACATGCCGGCACGCGCCGGCGACTTCGGCATCGAGCTCGTGGGCGGC
ATGCCCTGGCTGGTGCGGGCCAGCGCCGCCGACGAAACGCAGCGCGCGCGCCGCCGCCCCGGGCCGCCCGGCGCAGTCCC
CGCCCAAGAACTACAGATTCAGCGCCTGATCCCCGCCGACGCACTGCGCATCCGTGGCCGGCACAACGCCGTCAACGCGC
TGGCCGCGCTGGCGCTGGCCTGCGCCGCTGGCTGCCCGCTGGCGCCGATGCTGTACGGTCTGCGCGCCTACCGGGGCGAG
CCGCACCGTGTCGAATCCGTGGCCTGCATCGATGGCGTGGAGTTCTTCGACGACAGCAAGGGCACCAACGTGGGCGCCAC
GGTGGCGGCCATCGACGGGCTGGGCGCGCAGGGCGGGCCGGGCGCCGGGCGCCGCCTGGTCGTCATCGTGGGCGGCCAGG
GCAAGGGGCAGGACTTTGCGCCGCTGGCCGCGCCGCTGGCGCGCCACGCCCGCGCAGTGCTGCTGATCGGCTGCGACGCG
CCGCTGATCCGCGCCGCGCTGCAAGACATCGGCGTGCCGCTGCTGGACGCGCCCACCCTGCCCGGGGCCGTCGAACTGGC
CGCGCAGCACGCGCAGCCGGGCGACGCGGTGCTGATGTCGCCGGCCTGCGCCAGCTTCGACATGTTCCGGGACTACGAAC
AGCGCGCGCAGGTTTTTTGCGCCAGCGTGCGCGCATTGGCCGAGGCAGCCGGCCAGCCGATGGAGGGTCTGGCATGA

Upstream 100 bases:

>100_bases
ATTTCGAAAAAAGCGGCTGGAAGGAAACGCAGGTGGTGGTGCGCTTTTGGATCATCACCATGCTGCTGTGCCTGGTGGGC
CTGTCGACATTGAAGCTGCG

Downstream 100 bases:

>100_bases
ACATGCCGCGACTGCTGCAATGGATCACCGGCTGGTTCGACGGCCAGTCGGGCCAGGCTGCCGACCGGCTGCCGGTGCGC
GTGGGCGGCACCGAGTACCG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MNPNAQPTLPARPLDEAGAPVPEAPPGAPRVKPPVNPPVNPPAISAAQAAADFVAQIFASVPAPEEAAPAMPLAIDPAIN
PVINPAMPPAEVPGALAAEPLRRPGADLRQALQDQRILILGLGASGLAMARWCLRCGAQVTVADTRAAPPQLPALQRELP
RARFVPGAFDAGLVEGQNLHAVYCSPGLSPSAVAAVAQTCSAHGIGMGGELDLYALALAGLRAADGYAPTVLAITGTNGK
TTVTALTGQLLQRAGRSVALAGNIGPTLLDTLAGHIDAGTLPAVWVLELSSFQLERSTGFEPTAAALLNISQDHLDWHGD
MAAYAAAKARIFGQTALMVLNRQDAAVMQVSAPSAPSAPVTPALRPRAAKAPAPQPRADITFGADMPARAGDFGIELVGG
MPWLVRASAADETQRARRRPGPPGAVPAQELQIQRLIPADALRIRGRHNAVNALAALALACAAGCPLAPMLYGLRAYRGE
PHRVESVACIDGVEFFDDSKGTNVGATVAAIDGLGAQGGPGAGRRLVVIVGGQGKGQDFAPLAAPLARHARAVLLIGCDA
PLIRAALQDIGVPLLDAPTLPGAVELAAQHAQPGDAVLMSPACASFDMFRDYEQRAQVFCASVRALAEAAGQPMEGLA

Sequences:

>Translated_638_residues
MNPNAQPTLPARPLDEAGAPVPEAPPGAPRVKPPVNPPVNPPAISAAQAAADFVAQIFASVPAPEEAAPAMPLAIDPAIN
PVINPAMPPAEVPGALAAEPLRRPGADLRQALQDQRILILGLGASGLAMARWCLRCGAQVTVADTRAAPPQLPALQRELP
RARFVPGAFDAGLVEGQNLHAVYCSPGLSPSAVAAVAQTCSAHGIGMGGELDLYALALAGLRAADGYAPTVLAITGTNGK
TTVTALTGQLLQRAGRSVALAGNIGPTLLDTLAGHIDAGTLPAVWVLELSSFQLERSTGFEPTAAALLNISQDHLDWHGD
MAAYAAAKARIFGQTALMVLNRQDAAVMQVSAPSAPSAPVTPALRPRAAKAPAPQPRADITFGADMPARAGDFGIELVGG
MPWLVRASAADETQRARRRPGPPGAVPAQELQIQRLIPADALRIRGRHNAVNALAALALACAAGCPLAPMLYGLRAYRGE
PHRVESVACIDGVEFFDDSKGTNVGATVAAIDGLGAQGGPGAGRRLVVIVGGQGKGQDFAPLAAPLARHARAVLLIGCDA
PLIRAALQDIGVPLLDAPTLPGAVELAAQHAQPGDAVLMSPACASFDMFRDYEQRAQVFCASVRALAEAAGQPMEGLA
>Mature_638_residues
MNPNAQPTLPARPLDEAGAPVPEAPPGAPRVKPPVNPPVNPPAISAAQAAADFVAQIFASVPAPEEAAPAMPLAIDPAIN
PVINPAMPPAEVPGALAAEPLRRPGADLRQALQDQRILILGLGASGLAMARWCLRCGAQVTVADTRAAPPQLPALQRELP
RARFVPGAFDAGLVEGQNLHAVYCSPGLSPSAVAAVAQTCSAHGIGMGGELDLYALALAGLRAADGYAPTVLAITGTNGK
TTVTALTGQLLQRAGRSVALAGNIGPTLLDTLAGHIDAGTLPAVWVLELSSFQLERSTGFEPTAAALLNISQDHLDWHGD
MAAYAAAKARIFGQTALMVLNRQDAAVMQVSAPSAPSAPVTPALRPRAAKAPAPQPRADITFGADMPARAGDFGIELVGG
MPWLVRASAADETQRARRRPGPPGAVPAQELQIQRLIPADALRIRGRHNAVNALAALALACAAGCPLAPMLYGLRAYRGE
PHRVESVACIDGVEFFDDSKGTNVGATVAAIDGLGAQGGPGAGRRLVVIVGGQGKGQDFAPLAAPLARHARAVLLIGCDA
PLIRAALQDIGVPLLDAPTLPGAVELAAQHAQPGDAVLMSPACASFDMFRDYEQRAQVFCASVRALAEAAGQPMEGLA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=511, Percent_Identity=33.2681017612524, Blast_Score=200, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 65282; Mature: 65282

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPNAQPTLPARPLDEAGAPVPEAPPGAPRVKPPVNPPVNPPAISAAQAAADFVAQIFAS
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VPAPEEAAPAMPLAIDPAINPVINPAMPPAEVPGALAAEPLRRPGADLRQALQDQRILIL
CCCCCHHCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEE
GLGASGLAMARWCLRCGAQVTVADTRAAPPQLPALQRELPRARFVPGAFDAGLVEGQNLH
EECCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCHHHCCCCCCCCCCCCCCCEE
AVYCSPGLSPSAVAAVAQTCSAHGIGMGGELDLYALALAGLRAADGYAPTVLAITGTNGK
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
TTVTALTGQLLQRAGRSVALAGNIGPTLLDTLAGHIDAGTLPAVWVLELSSFQLERSTGF
EEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHEEHHCCCC
EPTAAALLNISQDHLDWHGDMAAYAAAKARIFGQTALMVLNRQDAAVMQVSAPSAPSAPV
CHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCCCC
TPALRPRAAKAPAPQPRADITFGADMPARAGDFGIELVGGMPWLVRASAADETQRARRRP
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHCCC
GPPGAVPAQELQIQRLIPADALRIRGRHNAVNALAALALACAAGCPLAPMLYGLRAYRGE
CCCCCCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
PHRVESVACIDGVEFFDDSKGTNVGATVAAIDGLGAQGGPGAGRRLVVIVGGQGKGQDFA
CCCCCEEHHHCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCC
PLAAPLARHARAVLLIGCDAPLIRAALQDIGVPLLDAPTLPGAVELAAQHAQPGDAVLMS
HHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCEEEEC
PACASFDMFRDYEQRAQVFCASVRALAEAAGQPMEGLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNPNAQPTLPARPLDEAGAPVPEAPPGAPRVKPPVNPPVNPPAISAAQAAADFVAQIFAS
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VPAPEEAAPAMPLAIDPAINPVINPAMPPAEVPGALAAEPLRRPGADLRQALQDQRILIL
CCCCCHHCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEE
GLGASGLAMARWCLRCGAQVTVADTRAAPPQLPALQRELPRARFVPGAFDAGLVEGQNLH
EECCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCHHHCCCCCCCCCCCCCCCEE
AVYCSPGLSPSAVAAVAQTCSAHGIGMGGELDLYALALAGLRAADGYAPTVLAITGTNGK
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
TTVTALTGQLLQRAGRSVALAGNIGPTLLDTLAGHIDAGTLPAVWVLELSSFQLERSTGF
EEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHEEHHCCCC
EPTAAALLNISQDHLDWHGDMAAYAAAKARIFGQTALMVLNRQDAAVMQVSAPSAPSAPV
CHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCCCC
TPALRPRAAKAPAPQPRADITFGADMPARAGDFGIELVGGMPWLVRASAADETQRARRRP
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHCCC
GPPGAVPAQELQIQRLIPADALRIRGRHNAVNALAALALACAAGCPLAPMLYGLRAYRGE
CCCCCCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
PHRVESVACIDGVEFFDDSKGTNVGATVAAIDGLGAQGGPGAGRRLVVIVGGQGKGQDFA
CCCCCEEHHHCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCC
PLAAPLARHARAVLLIGCDAPLIRAALQDIGVPLLDAPTLPGAVELAAQHAQPGDAVLMS
HHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCEEEEC
PACASFDMFRDYEQRAQVFCASVRALAEAAGQPMEGLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA