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The map label for this gene is hslU

Identifier: 121611469

GI number: 121611469

Start: 5048083

End: 5049408

Strand: Direct

Name: hslU

Synonym: Veis_4560

Alternate gene names: 121611469

Gene position: 5048083-5049408 (Clockwise)

Preceding gene: 121611468

Following gene: 121611470

Centisome position: 90.68

GC content: 63.95

Gene sequence:

>1326_bases
ATGACTTCGATGACCCCCCAGGAGATCGTCTCCGAGCTCGACAAGCACATCGTCGGCCAGTCGAGCGCCAAGCGCGCCGT
GGCGATTGCGCTGCGCAACCGCTGGCGCCGCCAGCAGGTGCAAGGCAGTCTGCGCCAGGAGATTACGCCCAAGAACATCC
TGATGATAGGCCCCACCGGCGTGGGCAAGACCGAAATTGCCCGCCGTCTGGCCAAACTGGCCGATGCGCCCTTCATCAAG
GTCGAGGCCACCAAATTCACCGAGGTGGGCTATGTGGGCAAGGATGTCGACTCCATCATCCGCGACCTGGCCGACATGGC
GGTCAAGCAGACGCGCGAGTCCGAGATGAAAAAGGTGCGCGCCCGCGCCGAGGACGCCGCCGAGGATCGCATCCTGGATG
TGCTGATCCCGCCGCCCCGGGGTGCTGACGGCGCCGAGCCGGCAGCCGACGGCACGACGCGGCAGATGTTTCGCAAAAAG
CTGCGCGAAGGCTTGCTCGACGACAAGGACATCGAGATCGACGTCACCGAAAGCCGCCCGCATCTGGAAATCATGGGCCC
CCAGGGCATGGAGGAAATGACCGAGCAACTGCGCAGCATGTTCAGCCAGTTCGGGCAGGACAAGCGCCGCACGCGCAAGC
TCAAGATCGCCGAGGCGATGAAACTGCTCACCGACGAAGAGGCCGGCAAGTTGGTCAACGAAGAGGAGATCAAGACCCGG
GCACTGGCCAATGCCGAGCAAAACGGCATCGTCTTCATCGACGAAATCGACAAGGTCGCCACGCGCCAGGAAACCAGCGG
TGCCGACGTGTCGCGCCAGGGCGTGCAGCGCGATCTGCTGCCGCTGGTCGAGGGCACCACGGTGTCGACCAAGTACGGGA
TGATCAAGACCGACCACATCCTGTTCATCGCCTCCGGGGCCTTTCACCTGTCGCGGCCCAGCGACCTGATTCCCGAGTTG
CAGGGGCGCTTTCCGATCCGGGTCGAGCTCGAATCGCTGTCGGTGCAGGACTTCGAGGCGATTTTGACCCAGACCCACGC
CTCGCTGGTCAAGCAATACCAGGCCCTGCTGGCGACCGAAGACGTGACGCTGGCGTTCGTGCCGCAAGGCATCACGCGGC
TGGCGTGCATCGCTTTCGAGGTCAACGAGCGCACCGAGAACATCGGCGCGCGCCGCCTGTCCACCGTGATGGAGCGGCTG
CTCGACGAGGTCAGTTTCGACGCCACGCGGCTGTCGGGCCAGACGGTCACCATCGATGAGGGCTATGTGGACCAGCGTCT
GCAGCAGCTCAGCCTGAACGAGGATCTGTCGCGCTACATCTTGTGA

Upstream 100 bases:

>100_bases
ATCGGGTGCGCGCCGGGGGCCGCAAATTACTCTTTTTCTCATAGCTACAGGCGCCTGATTCGTCTGCGCCTGTGCGTTGT
CCGATACCAGGAAAACCACC

Downstream 100 bases:

>100_bases
GTCCTGCCGGCTCCCAAGCTGAGACATCACTTTGACAAACTGATGTAAGGACTTACTTATCAACGGCATTTTTTTACGCC
CCCCTGCACAGGGGCGGCCA

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTSMTPQEIVSELDKHIVGQSSAKRAVAIALRNRWRRQQVQGSLRQEITPKNILMIGPTGVGKTEIARRLAKLADAPFIK
VEATKFTEVGYVGKDVDSIIRDLADMAVKQTRESEMKKVRARAEDAAEDRILDVLIPPPRGADGAEPAADGTTRQMFRKK
LREGLLDDKDIEIDVTESRPHLEIMGPQGMEEMTEQLRSMFSQFGQDKRRTRKLKIAEAMKLLTDEEAGKLVNEEEIKTR
ALANAEQNGIVFIDEIDKVATRQETSGADVSRQGVQRDLLPLVEGTTVSTKYGMIKTDHILFIASGAFHLSRPSDLIPEL
QGRFPIRVELESLSVQDFEAILTQTHASLVKQYQALLATEDVTLAFVPQGITRLACIAFEVNERTENIGARRLSTVMERL
LDEVSFDATRLSGQTVTIDEGYVDQRLQQLSLNEDLSRYIL

Sequences:

>Translated_441_residues
MTSMTPQEIVSELDKHIVGQSSAKRAVAIALRNRWRRQQVQGSLRQEITPKNILMIGPTGVGKTEIARRLAKLADAPFIK
VEATKFTEVGYVGKDVDSIIRDLADMAVKQTRESEMKKVRARAEDAAEDRILDVLIPPPRGADGAEPAADGTTRQMFRKK
LREGLLDDKDIEIDVTESRPHLEIMGPQGMEEMTEQLRSMFSQFGQDKRRTRKLKIAEAMKLLTDEEAGKLVNEEEIKTR
ALANAEQNGIVFIDEIDKVATRQETSGADVSRQGVQRDLLPLVEGTTVSTKYGMIKTDHILFIASGAFHLSRPSDLIPEL
QGRFPIRVELESLSVQDFEAILTQTHASLVKQYQALLATEDVTLAFVPQGITRLACIAFEVNERTENIGARRLSTVMERL
LDEVSFDATRLSGQTVTIDEGYVDQRLQQLSLNEDLSRYIL
>Mature_440_residues
TSMTPQEIVSELDKHIVGQSSAKRAVAIALRNRWRRQQVQGSLRQEITPKNILMIGPTGVGKTEIARRLAKLADAPFIKV
EATKFTEVGYVGKDVDSIIRDLADMAVKQTRESEMKKVRARAEDAAEDRILDVLIPPPRGADGAEPAADGTTRQMFRKKL
REGLLDDKDIEIDVTESRPHLEIMGPQGMEEMTEQLRSMFSQFGQDKRRTRKLKIAEAMKLLTDEEAGKLVNEEEIKTRA
LANAEQNGIVFIDEIDKVATRQETSGADVSRQGVQRDLLPLVEGTTVSTKYGMIKTDHILFIASGAFHLSRPSDLIPELQ
GRFPIRVELESLSVQDFEAILTQTHASLVKQYQALLATEDVTLAFVPQGITRLACIAFEVNERTENIGARRLSTVMERLL
DEVSFDATRLSGQTVTIDEGYVDQRLQQLSLNEDLSRYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=67.0403587443946, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1786642, Length=100, Percent_Identity=47, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_VEREI (A1WRK1)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_999276.1
- ProteinModelPortal:   A1WRK1
- SMR:   A1WRK1
- STRING:   A1WRK1
- GeneID:   4694601
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4560
- NMPDR:   fig|391735.5.peg.4180
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- PhylomeDB:   A1WRK1
- ProtClustDB:   PRK05201
- BioCyc:   VEIS391735:VEIS_4560-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2

EC number: NA

Molecular weight: Translated: 49358; Mature: 49227

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 254-254 BINDING 319-319 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSMTPQEIVSELDKHIVGQSSAKRAVAIALRNRWRRQQVQGSLRQEITPKNILMIGPTG
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLADAPFIKVEATKFTEVGYVGKDVDSIIRDLADMAVKQTRESEMKKVR
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARAEDAAEDRILDVLIPPPRGADGAEPAADGTTRQMFRKKLREGLLDDKDIEIDVTESRP
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCC
HLEIMGPQGMEEMTEQLRSMFSQFGQDKRRTRKLKIAEAMKLLTDEEAGKLVNEEEIKTR
CEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALANAEQNGIVFIDEIDKVATRQETSGADVSRQGVQRDLLPLVEGTTVSTKYGMIKTDHI
HHHCCCCCCEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCEEEECCE
LFIASGAFHLSRPSDLIPELQGRFPIRVELESLSVQDFEAILTQTHASLVKQYQALLATE
EEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DVTLAFVPQGITRLACIAFEVNERTENIGARRLSTVMERLLDEVSFDATRLSGQTVTIDE
CCEEEECCCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEECC
GYVDQRLQQLSLNEDLSRYIL
CHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSMTPQEIVSELDKHIVGQSSAKRAVAIALRNRWRRQQVQGSLRQEITPKNILMIGPTG
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLADAPFIKVEATKFTEVGYVGKDVDSIIRDLADMAVKQTRESEMKKVR
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARAEDAAEDRILDVLIPPPRGADGAEPAADGTTRQMFRKKLREGLLDDKDIEIDVTESRP
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCC
HLEIMGPQGMEEMTEQLRSMFSQFGQDKRRTRKLKIAEAMKLLTDEEAGKLVNEEEIKTR
CEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALANAEQNGIVFIDEIDKVATRQETSGADVSRQGVQRDLLPLVEGTTVSTKYGMIKTDHI
HHHCCCCCCEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCEEEECCE
LFIASGAFHLSRPSDLIPELQGRFPIRVELESLSVQDFEAILTQTHASLVKQYQALLATE
EEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DVTLAFVPQGITRLACIAFEVNERTENIGARRLSTVMERLLDEVSFDATRLSGQTVTIDE
CCEEEECCCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEECC
GYVDQRLQQLSLNEDLSRYIL
CHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA