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The map label for this gene is rpoH [H]

Identifier: 121611388

GI number: 121611388

Start: 4946890

End: 4947831

Strand: Direct

Name: rpoH [H]

Synonym: Veis_4476

Alternate gene names: 121611388

Gene position: 4946890-4947831 (Clockwise)

Preceding gene: 121611387

Following gene: 121611390

Centisome position: 88.86

GC content: 64.33

Gene sequence:

>942_bases
ATGACCATTCAATCCGGCTCCGCAACGCTGAGCCTGGCTCCCGCCAACCCTTGGACGCTGGTGCCTGCGCTGGGCAACCT
CGACGCTTATATTGCCGCCGTCAACCGCCTGCCCCTGCTCACGTATGAGCAAGAGCAAAGCTACGCGCGCCAGTTCAAGG
AACACAACGATCTGGACGCCGCTGGCCGGCTGGTGCTGTCGCACCTGCGCCTGGTGGTGTCGATTGCGCGCCAGTACCTG
GGCTACGGCCTGCCGCACGGCGACCTGATACAGGAAGGCAATATCGGCCTGATGAAAGCCGTCAAGCGCTTCGACCCCGA
CCAGGGCGTGCGCCTGGTCAGCTACGCCATGCACTGGATCAAGGCCGAGATCCACGAATACATCCTGAAGAACTGGCGCA
TGGTCAAGGTGGCAACCACCAAGGCCCAGCGCAAGCTGTTCTTCAACCTGCGCTCGATGAAGCAGGGCTTCAAGGCCGAC
GCCGGCGCCACCGATGCTGCCACCTACCGCGACACGCTGTCCGAGCACGAAATCGACGCCGTCGCGGCGCAGCTCCATGT
CAAGCGCGAAGAAGTCATCGAGATGGAAACCCGCATGTCCGGCGGCGATGTGCTGCTCGACCCGGCGCCATCGGACGATG
GCGAGCAGGCCTTTGGCCCGATTGCCTACCTGGCCGATGCCTCGCACGAGCCGACGGCGCTGATCGAGTCGCGCCAGCGG
GATGCGCTGGCCACCGATGGCATTGCCAAGGCGCTGGCCAGCCTGGACGAGCGCAGCCGGCGCATCGTGCAAGAGCGCTG
GCTCAAGGTCAACGATGACGGAACGGGCGGCATGACGCTGCACGATCTGGCCGCCATTTACGGCGTGAGCGCCGAACGCA
TCCGCCAGATCGAGGTGGCGGCCATGAAGAAGATGAAGCAGGCGCTGGTCAAATACGCCTGA

Upstream 100 bases:

>100_bases
GAACGCAGCGGCCGACGGAACTCCGGCCTTGGCACTCGATCGCAGAGAGTGCTAACATTCCCCCACTTGGGAAGATTGGG
CAGAGGAAAGGATTCTTGCA

Downstream 100 bases:

>100_bases
GCTTGCCCAAGCGCCCAAGCGAATACGCCGGCAAAGAGTTGCCTCACGGGGCCCCTGACCGGGCCCCGCGCCGTTTCTGC
CCCGAAGATTCAGCAACCAT

Product: RNA polymerase sigma-32 subunit RpoH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTIQSGSATLSLAPANPWTLVPALGNLDAYIAAVNRLPLLTYEQEQSYARQFKEHNDLDAAGRLVLSHLRLVVSIARQYL
GYGLPHGDLIQEGNIGLMKAVKRFDPDQGVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQGFKAD
AGATDAATYRDTLSEHEIDAVAAQLHVKREEVIEMETRMSGGDVLLDPAPSDDGEQAFGPIAYLADASHEPTALIESRQR
DALATDGIAKALASLDERSRRIVQERWLKVNDDGTGGMTLHDLAAIYGVSAERIRQIEVAAMKKMKQALVKYA

Sequences:

>Translated_313_residues
MTIQSGSATLSLAPANPWTLVPALGNLDAYIAAVNRLPLLTYEQEQSYARQFKEHNDLDAAGRLVLSHLRLVVSIARQYL
GYGLPHGDLIQEGNIGLMKAVKRFDPDQGVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQGFKAD
AGATDAATYRDTLSEHEIDAVAAQLHVKREEVIEMETRMSGGDVLLDPAPSDDGEQAFGPIAYLADASHEPTALIESRQR
DALATDGIAKALASLDERSRRIVQERWLKVNDDGTGGMTLHDLAAIYGVSAERIRQIEVAAMKKMKQALVKYA
>Mature_312_residues
TIQSGSATLSLAPANPWTLVPALGNLDAYIAAVNRLPLLTYEQEQSYARQFKEHNDLDAAGRLVLSHLRLVVSIARQYLG
YGLPHGDLIQEGNIGLMKAVKRFDPDQGVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQGFKADA
GATDAATYRDTLSEHEIDAVAAQLHVKREEVIEMETRMSGGDVLLDPAPSDDGEQAFGPIAYLADASHEPTALIESRQRD
ALATDGIAKALASLDERSRRIVQERWLKVNDDGTGGMTLHDLAAIYGVSAERIRQIEVAAMKKMKQALVKYA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=302, Percent_Identity=49.0066225165563, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1789098, Length=282, Percent_Identity=29.4326241134752, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789448, Length=276, Percent_Identity=30.4347826086957, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34712; Mature: 34581

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQSGSATLSLAPANPWTLVPALGNLDAYIAAVNRLPLLTYEQEQSYARQFKEHNDLDA
CCCCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCH
AGRLVLSHLRLVVSIARQYLGYGLPHGDLIQEGNIGLMKAVKRFDPDQGVRLVSYAMHWI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQGFKADAGATDAATYRDTLSEHEIDA
HHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
VAAQLHVKREEVIEMETRMSGGDVLLDPAPSDDGEQAFGPIAYLADASHEPTALIESRQR
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHCHHHHHHCCCCCCHHHHHHHHH
DALATDGIAKALASLDERSRRIVQERWLKVNDDGTGGMTLHDLAAIYGVSAERIRQIEVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
AMKKMKQALVKYA
HHHHHHHHHHHCC
>Mature Secondary Structure 
TIQSGSATLSLAPANPWTLVPALGNLDAYIAAVNRLPLLTYEQEQSYARQFKEHNDLDA
CCCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCH
AGRLVLSHLRLVVSIARQYLGYGLPHGDLIQEGNIGLMKAVKRFDPDQGVRLVSYAMHWI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQGFKADAGATDAATYRDTLSEHEIDA
HHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
VAAQLHVKREEVIEMETRMSGGDVLLDPAPSDDGEQAFGPIAYLADASHEPTALIESRQR
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHCHHHHHHCCCCCCHHHHHHHHH
DALATDGIAKALASLDERSRRIVQERWLKVNDDGTGGMTLHDLAAIYGVSAERIRQIEVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
AMKKMKQALVKYA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]