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The map label for this gene is hisF [H]

Identifier: 121611299

GI number: 121611299

Start: 4831955

End: 4832194

Strand: Direct

Name: hisF [H]

Synonym: Veis_4385

Alternate gene names: 121611299

Gene position: 4831955-4832194 (Clockwise)

Preceding gene: 121611298

Following gene: 121611302

Centisome position: 86.8

GC content: 67.5

Gene sequence:

>240_bases
ATGGACCGCGACGGCACCAGAATCGGCTTTGATCTGGAGCTGACACGCGCCGTCAACGACGCCGTGGGCCTGCCGGTGAT
CGCCTCGGGCGGCGTGGGCACACTCGATCATCTGGCCGATGGCATACAGACCGGCGGCGCCGACGCCGTGCTGGCCGCCA
GCATCTTCCATTACGGTGAATACACGGTGGGCCAGGCCAAGCAGCATCTGGCCGGACGCGGCATTGCGGTGCGGCGCTGA

Upstream 100 bases:

>100_bases
CAGGGCAAGTCCACCCTAGTCGCAGTAGCGTTGGCCACCATCGCACGCTTCAACAAATAAGGTGCTGCCCCGTGCGGCAC
CGCTGGTGATGGACGGAGGC

Downstream 100 bases:

>100_bases
TGCGGCGCAATGGCCGCCCCGGGCTGGCGCCCGGGGTCAATGGGCCGGCGCCGGCAGGCATTTGAATGCCAGCGCCCCCA
AGACCAGCATCAGCGCAATG

Product: imidazoleglycerol phosphate synthase, cyclase subunit

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 79; Mature: 79

Protein sequence:

>79_residues
MDRDGTRIGFDLELTRAVNDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAASIFHYGEYTVGQAKQHLAGRGIAVRR

Sequences:

>Translated_79_residues
MDRDGTRIGFDLELTRAVNDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAASIFHYGEYTVGQAKQHLAGRGIAVRR
>Mature_79_residues
MDRDGTRIGFDLELTRAVNDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAASIFHYGEYTVGQAKQHLAGRGIAVRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=78, Percent_Identity=39.7435897435897, Blast_Score=70, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=78, Percent_Identity=46.1538461538462, Blast_Score=76, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 8161; Mature: 8161

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRDGTRIGFDLELTRAVNDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAASIFHYGE
CCCCCCEEECEEEHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCC
YTVGQAKQHLAGRGIAVRR
CCHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MDRDGTRIGFDLELTRAVNDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAASIFHYGE
CCCCCCEEECEEEHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCC
YTVGQAKQHLAGRGIAVRR
CCHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA