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The map label for this gene is thiDE [H]

Identifier: 121611229

GI number: 121611229

Start: 4756562

End: 4757455

Strand: Direct

Name: thiDE [H]

Synonym: Veis_4315

Alternate gene names: 121611229

Gene position: 4756562-4757455 (Clockwise)

Preceding gene: 121611224

Following gene: 121611240

Centisome position: 85.45

GC content: 68.79

Gene sequence:

>894_bases
ATGACTTCATCGACTCCGCTGCCGGGCGCGCCGGGCGCATCGGACGCAATACGCCGCTATGCCCGTGTGCTCTCGATTGC
CGGCTCCGACAGCGGTGGCGGGGCGGGGATACAGGCCGACCTCAAGACCTTCAGCGCTTTGGGCTGTTATGGCATGACGG
CGATCACGGCGATCACGGCGCAAAACACCTGCGCGGTGACGGGCATCCACGGCATTCCCCCGGAGATGCTCAAAGCCCAG
ATCGACGCAGTGGTGCAGGACATTGGTGTCGATGCGGTCAAGATCGGCATGCTGCATTCGCCCGCCGTGGTGCGCGTGGC
GGCCGATGCCATCCGCAGCCATCGGCTGCCGCAGGTGGTGCTCGATCCGGTGATGGTGACCAGCAGCGGCGACCGCCTGA
TGGCCCAGGAGACGGTGGGCGTGCTGGTGCAGGAACTGTTTCCGCTGGCCGTGCTGATCACCCCCAATCTGGACGAGGCC
GGCTGGCTGCTGGGCCGCAGCATCGACGGCGTGCCTGCGCTGGAACAGGCGGCGCAGGATCTGCTGCGCTTGGGCGCCCG
CGCCGTGCTGCTCAAAGGCGGGCATTTGCCGGGCGCGCAGGTCGTGGATGTGCTGGCCGTGCAGGGCGCGCCGCTCCTGT
GCATGCAGTCCCCGCGCATTGCCACGCACAACGGCCATGGCACGGGCTGCACGCTGTCGTCCGCCATTGCCGCGCATCTG
GCGCTGGGCCGGCCGCTGCCGCAGGCGGTGGAACAGGCCCGCAGCTACATTCTGCAGGCCATGGCGGCGGGCGCGCAGGT
GACCACCGGGCATGGCCATGGGCCGCTGAACCATGGCTACGCCCCCCTGGTGCAGCAGAGCTTTGCCGTCATCGCTGGTG
CGGGCAACGGTTAG

Upstream 100 bases:

>100_bases
GCGGTGTGTCTGTGTCTGTGTCTGTCTGTGTGCTTACAGGCAGTACAGCATACCCTCTGCGGGCGGCGGTCGATTTGGCG
CATGGGCCATAATTTTGGCC

Downstream 100 bases:

>100_bases
GCGGGACCGGCAGCGCTGTGGAAGGCCGGCCCGGTGGCCATCGATCGCGCGGGGCCTCTACGGCCATTCCGATCAAACAG
GCCCTGCGGCCATCCCAACC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTSSTPLPGAPGASDAIRRYARVLSIAGSDSGGGAGIQADLKTFSALGCYGMTAITAITAQNTCAVTGIHGIPPEMLKAQ
IDAVVQDIGVDAVKIGMLHSPAVVRVAADAIRSHRLPQVVLDPVMVTSSGDRLMAQETVGVLVQELFPLAVLITPNLDEA
GWLLGRSIDGVPALEQAAQDLLRLGARAVLLKGGHLPGAQVVDVLAVQGAPLLCMQSPRIATHNGHGTGCTLSSAIAAHL
ALGRPLPQAVEQARSYILQAMAAGAQVTTGHGHGPLNHGYAPLVQQSFAVIAGAGNG

Sequences:

>Translated_297_residues
MTSSTPLPGAPGASDAIRRYARVLSIAGSDSGGGAGIQADLKTFSALGCYGMTAITAITAQNTCAVTGIHGIPPEMLKAQ
IDAVVQDIGVDAVKIGMLHSPAVVRVAADAIRSHRLPQVVLDPVMVTSSGDRLMAQETVGVLVQELFPLAVLITPNLDEA
GWLLGRSIDGVPALEQAAQDLLRLGARAVLLKGGHLPGAQVVDVLAVQGAPLLCMQSPRIATHNGHGTGCTLSSAIAAHL
ALGRPLPQAVEQARSYILQAMAAGAQVTTGHGHGPLNHGYAPLVQQSFAVIAGAGNG
>Mature_296_residues
TSSTPLPGAPGASDAIRRYARVLSIAGSDSGGGAGIQADLKTFSALGCYGMTAITAITAQNTCAVTGIHGIPPEMLKAQI
DAVVQDIGVDAVKIGMLHSPAVVRVAADAIRSHRLPQVVLDPVMVTSSGDRLMAQETVGVLVQELFPLAVLITPNLDEAG
WLLGRSIDGVPALEQAAQDLLRLGARAVLLKGGHLPGAQVVDVLAVQGAPLLCMQSPRIATHNGHGTGCTLSSAIAAHLA
LGRPLPQAVEQARSYILQAMAAGAQVTTGHGHGPLNHGYAPLVQQSFAVIAGAGNG

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=260, Percent_Identity=44.2307692307692, Blast_Score=195, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6325378, Length=270, Percent_Identity=36.2962962962963, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324997, Length=297, Percent_Identity=36.7003367003367, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=38.2352941176471, Blast_Score=155, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 30199; Mature: 30068

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSTPLPGAPGASDAIRRYARVLSIAGSDSGGGAGIQADLKTFSALGCYGMTAITAITA
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
QNTCAVTGIHGIPPEMLKAQIDAVVQDIGVDAVKIGMLHSPAVVRVAADAIRSHRLPQVV
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCHHH
LDPVMVTSSGDRLMAQETVGVLVQELFPLAVLITPNLDEAGWLLGRSIDGVPALEQAAQD
HCCEEEECCCCCHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHH
LLRLGARAVLLKGGHLPGAQVVDVLAVQGAPLLCMQSPRIATHNGHGTGCTLSSAIAAHL
HHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCHHHHHHHHHH
ALGRPLPQAVEQARSYILQAMAAGAQVTTGHGHGPLNHGYAPLVQQSFAVIAGAGNG
HHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHCCEEEEECCCC
>Mature Secondary Structure 
TSSTPLPGAPGASDAIRRYARVLSIAGSDSGGGAGIQADLKTFSALGCYGMTAITAITA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
QNTCAVTGIHGIPPEMLKAQIDAVVQDIGVDAVKIGMLHSPAVVRVAADAIRSHRLPQVV
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCHHH
LDPVMVTSSGDRLMAQETVGVLVQELFPLAVLITPNLDEAGWLLGRSIDGVPALEQAAQD
HCCEEEECCCCCHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHH
LLRLGARAVLLKGGHLPGAQVVDVLAVQGAPLLCMQSPRIATHNGHGTGCTLSSAIAAHL
HHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCHHHHHHHHHH
ALGRPLPQAVEQARSYILQAMAAGAQVTTGHGHGPLNHGYAPLVQQSFAVIAGAGNG
HHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA