Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 121611177

GI number: 121611177

Start: 4699018

End: 4700463

Strand: Direct

Name: pmbA [H]

Synonym: Veis_4261

Alternate gene names: 121611177

Gene position: 4699018-4700463 (Clockwise)

Preceding gene: 121611174

Following gene: 121611187

Centisome position: 84.41

GC content: 66.11

Gene sequence:

>1446_bases
ATGAACCCACCCACCGACCACACCCCCAGCACGGCGGCCGCTGCGCCAGCGCATCGCCCCGAGCATCGCCCCGAGCATCG
TCCCGACAGCGGCTTCAGTTACAGCCGCCCATTTTTTGAAGAGCTGGTAGACCGCGCCCTGGCCCATGCCAAAAAGCTCG
GCGCCACCGATGCCGCAGCCGAAGCCTCCGAGGGTTGCGGCCTGTCGGTCAGCGTGCGCAAAGGTGAGTTGGAAAACGTC
GAGCGCAACCGCGACAAATCGCTGGGCGTGACGGTCTACATGGCCCACCGGCGCGGCCAGGCCAGCACCTCGGACTTTTC
CACCCGGGCCATAGAGCAGACCGTGCAGGCCGCCTACGACATCGCCCGCTTCACCGCCGAAGACCCGATGGCCGGCCTGC
CCGACGAAGCCGATATCGCCCCGCCCGCCAGCCATCGTGATCTGGCCCTGTTCCACCCCTGGCCCATCACCAGCGAAGAA
GCCGCCCGCCTGGCGCTGGAATGCGAGGCCGCAGCACTCCAGACCAGCCGCCGCATCAGCAACAGCGAAGGCGCCAGCGT
ATCGGCCCAGCAAAGCCATTTCTTCAGCGCCCACACGCGCGGCTTTCGCGGCGGCTATGCCTCCTCGCGCCACAGCGTTT
CGGTGGCCCCCATCGCCAAACTGCCCGGCAAAAACGCCGAAATGCAGCGCGACGCCTGGTACAGCTCGATGCGCGACGCC
GCCCGACTGGCCTCGCCCCAGGCCATAGGCCGCTACGCCGCCGAACGCGCCCTGAGCCGCCTGGGCAGCCGCAAGATCGC
CACCACGCAATGCCCGGTGCTGTTCGAGGCCACGCTGGCCGCCGGCCTGCTCGGCGGCTTTGTGCAGGCCATCAGCGGCA
GCGCGCTGTACCGCAAAAGCAGCTTTTTGCCCAATTCGCTCGGCAAAATGATCTTTCCCCTGCATATCGACATCCTGGAA
GACCCCTTTGTCCCGGGCGGCAAAGGCAGCGCGCCATTCGACGACGAAGGCGTGCGCGTAGCGCCCCGCAAGGTGGTCAA
GCACGGGCGTGTGCAAGGCTACTTCCTGTCTTGCTACTCGGCCCGCAAACTGGGCCTGCAAACCACGGGCAATGCCGGCG
GTTCGCACAACCTGACACTCACATCGCGCCTGACCCAACCCGGCGACGATCTCGACGCGATGCTGCAAAAACTGGGCACC
GGGCTGTTCGTGATCGAACTGATGGGCCAGGGTCTGAACCCGGTCACCGGCGACTATTCACGCGGCGCCAGCGGCTTTTG
GGTAGAGCGCGGCCAGATTGCCTACCCGGTGCATGAAATCACCATCGCCGGCAACCTCAAGAACATGCTCAGGGGCATAG
CGGCCGTGGGAGCGGATGCCTACAACTACGGCGCCAAGACCGTGGGCTCGATTCTCGTGGAGCGGATGAAGGTGGCGGGG
AGTTGA

Upstream 100 bases:

>100_bases
GGCACAGCACGCAAAAAACGCGCAGAAAACGCACACCGGGGGCGCAAGGAAACGGGCGCAAAAAAGGCGCTGCGCAGCGG
CAAGTATCATAGCGGCCGCC

Downstream 100 bases:

>100_bases
CATTTCCCCGCATGTTGCGCCTGTGCTTTTGATGCTTTTGATCTTCCGGATGCTTCGGGGCTTGGTGCGCGGGTCGGTGG
TGCCCAGGGCCATGGCGCCA

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MNPPTDHTPSTAAAAPAHRPEHRPEHRPDSGFSYSRPFFEELVDRALAHAKKLGATDAAAEASEGCGLSVSVRKGELENV
ERNRDKSLGVTVYMAHRRGQASTSDFSTRAIEQTVQAAYDIARFTAEDPMAGLPDEADIAPPASHRDLALFHPWPITSEE
AARLALECEAAALQTSRRISNSEGASVSAQQSHFFSAHTRGFRGGYASSRHSVSVAPIAKLPGKNAEMQRDAWYSSMRDA
ARLASPQAIGRYAAERALSRLGSRKIATTQCPVLFEATLAAGLLGGFVQAISGSALYRKSSFLPNSLGKMIFPLHIDILE
DPFVPGGKGSAPFDDEGVRVAPRKVVKHGRVQGYFLSCYSARKLGLQTTGNAGGSHNLTLTSRLTQPGDDLDAMLQKLGT
GLFVIELMGQGLNPVTGDYSRGASGFWVERGQIAYPVHEITIAGNLKNMLRGIAAVGADAYNYGAKTVGSILVERMKVAG
S

Sequences:

>Translated_481_residues
MNPPTDHTPSTAAAAPAHRPEHRPEHRPDSGFSYSRPFFEELVDRALAHAKKLGATDAAAEASEGCGLSVSVRKGELENV
ERNRDKSLGVTVYMAHRRGQASTSDFSTRAIEQTVQAAYDIARFTAEDPMAGLPDEADIAPPASHRDLALFHPWPITSEE
AARLALECEAAALQTSRRISNSEGASVSAQQSHFFSAHTRGFRGGYASSRHSVSVAPIAKLPGKNAEMQRDAWYSSMRDA
ARLASPQAIGRYAAERALSRLGSRKIATTQCPVLFEATLAAGLLGGFVQAISGSALYRKSSFLPNSLGKMIFPLHIDILE
DPFVPGGKGSAPFDDEGVRVAPRKVVKHGRVQGYFLSCYSARKLGLQTTGNAGGSHNLTLTSRLTQPGDDLDAMLQKLGT
GLFVIELMGQGLNPVTGDYSRGASGFWVERGQIAYPVHEITIAGNLKNMLRGIAAVGADAYNYGAKTVGSILVERMKVAG
S
>Mature_481_residues
MNPPTDHTPSTAAAAPAHRPEHRPEHRPDSGFSYSRPFFEELVDRALAHAKKLGATDAAAEASEGCGLSVSVRKGELENV
ERNRDKSLGVTVYMAHRRGQASTSDFSTRAIEQTVQAAYDIARFTAEDPMAGLPDEADIAPPASHRDLALFHPWPITSEE
AARLALECEAAALQTSRRISNSEGASVSAQQSHFFSAHTRGFRGGYASSRHSVSVAPIAKLPGKNAEMQRDAWYSSMRDA
ARLASPQAIGRYAAERALSRLGSRKIATTQCPVLFEATLAAGLLGGFVQAISGSALYRKSSFLPNSLGKMIFPLHIDILE
DPFVPGGKGSAPFDDEGVRVAPRKVVKHGRVQGYFLSCYSARKLGLQTTGNAGGSHNLTLTSRLTQPGDDLDAMLQKLGT
GLFVIELMGQGLNPVTGDYSRGASGFWVERGQIAYPVHEITIAGNLKNMLRGIAAVGADAYNYGAKTVGSILVERMKVAG
S

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=446, Percent_Identity=47.7578475336323, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 51431; Mature: 51431

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPTDHTPSTAAAAPAHRPEHRPEHRPDSGFSYSRPFFEELVDRALAHAKKLGATDAAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
EASEGCGLSVSVRKGELENVERNRDKSLGVTVYMAHRRGQASTSDFSTRAIEQTVQAAYD
HHCCCCCEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
IARFTAEDPMAGLPDEADIAPPASHRDLALFHPWPITSEEAARLALECEAAALQTSRRIS
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NSEGASVSAQQSHFFSAHTRGFRGGYASSRHSVSVAPIAKLPGKNAEMQRDAWYSSMRDA
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHCCCCCCCHHHHHHHHHHHHH
ARLASPQAIGRYAAERALSRLGSRKIATTQCPVLFEATLAAGLLGGFVQAISGSALYRKS
HHHCCHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SFLPNSLGKMIFPLHIDILEDPFVPGGKGSAPFDDEGVRVAPRKVVKHGRVQGYFLSCYS
CCCCHHHCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEECHHHHHHCCCCCEEEEEEHH
ARKLGLQTTGNAGGSHNLTLTSRLTQPGDDLDAMLQKLGTGLFVIELMGQGLNPVTGDYS
HHHCCCEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEHHHCCCCCCCCCCCC
RGASGFWVERGQIAYPVHEITIAGNLKNMLRGIAAVGADAYNYGAKTVGSILVERMKVAG
CCCCCEEEECCCEEECEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
S
C
>Mature Secondary Structure
MNPPTDHTPSTAAAAPAHRPEHRPEHRPDSGFSYSRPFFEELVDRALAHAKKLGATDAAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
EASEGCGLSVSVRKGELENVERNRDKSLGVTVYMAHRRGQASTSDFSTRAIEQTVQAAYD
HHCCCCCEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
IARFTAEDPMAGLPDEADIAPPASHRDLALFHPWPITSEEAARLALECEAAALQTSRRIS
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NSEGASVSAQQSHFFSAHTRGFRGGYASSRHSVSVAPIAKLPGKNAEMQRDAWYSSMRDA
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHCCCCCCCHHHHHHHHHHHHH
ARLASPQAIGRYAAERALSRLGSRKIATTQCPVLFEATLAAGLLGGFVQAISGSALYRKS
HHHCCHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SFLPNSLGKMIFPLHIDILEDPFVPGGKGSAPFDDEGVRVAPRKVVKHGRVQGYFLSCYS
CCCCHHHCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEECHHHHHHCCCCCEEEEEEHH
ARKLGLQTTGNAGGSHNLTLTSRLTQPGDDLDAMLQKLGTGLFVIELMGQGLNPVTGDYS
HHHCCCEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEHHHCCCCCCCCCCCC
RGASGFWVERGQIAYPVHEITIAGNLKNMLRGIAAVGADAYNYGAKTVGSILVERMKVAG
CCCCCEEEECCCEEECEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]