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The map label for this gene is trmJ [H]

Identifier: 121611165

GI number: 121611165

Start: 4687303

End: 4688103

Strand: Direct

Name: trmJ [H]

Synonym: Veis_4249

Alternate gene names: 121611165

Gene position: 4687303-4688103 (Clockwise)

Preceding gene: 121611163

Following gene: 121611166

Centisome position: 84.2

GC content: 65.67

Gene sequence:

>801_bases
ATGAAGACCCGCTTCGTCCTGATCAACACCAGCCACGCCGGCAATGTGGGCGCAGTCGCCCGCGCGATGAAAACCATGGG
TTTTGACGATCTGGTGCTGGTGGCCCCACGCTGGCCCAACGTGCTGCGGCACGAAGAAACCATACAGCGCGCCAGCGGCG
CCCTGGACCTGCTCCGGCAGGCCCGCATCGTTGCCGGATTGGACCAGGCCCTCGATGGCATCAGCCACCTGTGCGCCACC
GCGATGACGGCGCGCGACTTCGGACCGCCAACGACAACGCCGCGCGCGCATTTCGAGACGCTGCTGAAAAAAGAGCGCCC
AGTGCATGATTCATCTGCGCCAGCGGCCACCCGGCCGACGGGTGTGGCGTTCCTCTTCGGCTCCGAACGCTTTGGCATGA
GCAACGCCGATGTCTACCGCTGCCATGTGACGCTATCGATTCCCACCCAACCCGGGTTCGGCTCGCTGAACCTGGGCGCA
GCCGTCCAGGTCATTGCCTACGAATGGCGCATGGCCCTGGACGGCTGCGGCTTGGCCGTGCCGGACGGCACCCCGGCGCG
CCACCTGGCCGATGCCGTGCAGGTGGCGGGCATGCTGGAGCATTGGGAGCAGGCACTCACGCAGATCGGCTTTCTGGATC
CCGCCGCGCCCAAAAAGCTCATGCCGCGCCTGCAGCAATTGTTCAACCGCGCACAACTGGCTCCCGAGGAAATCCACATC
CTGCGCGGTATCGCCAAGGCCATCATCGCGACGGCGCAGGCCAACCGCCAACGCCCATCCCCGTCAAGCGACGAAACATG
A

Upstream 100 bases:

>100_bases
ATGATGGTGGGCAGGGCATCCTGCAAAGCAAAAGCGCCGGCGGAGGGGTTTTCCCGGCAATGCAGGCGGCGACAATCAGC
GCGATCTTACCTGCCCGCCC

Downstream 100 bases:

>100_bases
GTGCGCCCCGTCTCGCGACGAGCACGGCGACAGCGTGTCCGCGCCGAACACGTGATCCGCTTTCTCTACCCTCTCACGAT
TTCCGATGCGCCGTTCGACC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKTRFVLINTSHAGNVGAVARAMKTMGFDDLVLVAPRWPNVLRHEETIQRASGALDLLRQARIVAGLDQALDGISHLCAT
AMTARDFGPPTTTPRAHFETLLKKERPVHDSSAPAATRPTGVAFLFGSERFGMSNADVYRCHVTLSIPTQPGFGSLNLGA
AVQVIAYEWRMALDGCGLAVPDGTPARHLADAVQVAGMLEHWEQALTQIGFLDPAAPKKLMPRLQQLFNRAQLAPEEIHI
LRGIAKAIIATAQANRQRPSPSSDET

Sequences:

>Translated_266_residues
MKTRFVLINTSHAGNVGAVARAMKTMGFDDLVLVAPRWPNVLRHEETIQRASGALDLLRQARIVAGLDQALDGISHLCAT
AMTARDFGPPTTTPRAHFETLLKKERPVHDSSAPAATRPTGVAFLFGSERFGMSNADVYRCHVTLSIPTQPGFGSLNLGA
AVQVIAYEWRMALDGCGLAVPDGTPARHLADAVQVAGMLEHWEQALTQIGFLDPAAPKKLMPRLQQLFNRAQLAPEEIHI
LRGIAKAIIATAQANRQRPSPSSDET
>Mature_266_residues
MKTRFVLINTSHAGNVGAVARAMKTMGFDDLVLVAPRWPNVLRHEETIQRASGALDLLRQARIVAGLDQALDGISHLCAT
AMTARDFGPPTTTPRAHFETLLKKERPVHDSSAPAATRPTGVAFLFGSERFGMSNADVYRCHVTLSIPTQPGFGSLNLGA
AVQVIAYEWRMALDGCGLAVPDGTPARHLADAVQVAGMLEHWEQALTQIGFLDPAAPKKLMPRLQQLFNRAQLAPEEIHI
LRGIAKAIIATAQANRQRPSPSSDET

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=258, Percent_Identity=40.3100775193798, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790865, Length=228, Percent_Identity=28.5087719298246, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28767; Mature: 28767

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRFVLINTSHAGNVGAVARAMKTMGFDDLVLVAPRWPNVLRHEETIQRASGALDLLRQ
CCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ARIVAGLDQALDGISHLCATAMTARDFGPPTTTPRAHFETLLKKERPVHDSSAPAATRPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
GVAFLFGSERFGMSNADVYRCHVTLSIPTQPGFGSLNLGAAVQVIAYEWRMALDGCGLAV
CEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEC
PDGTPARHLADAVQVAGMLEHWEQALTQIGFLDPAAPKKLMPRLQQLFNRAQLAPEEIHI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
LRGIAKAIIATAQANRQRPSPSSDET
HHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKTRFVLINTSHAGNVGAVARAMKTMGFDDLVLVAPRWPNVLRHEETIQRASGALDLLRQ
CCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ARIVAGLDQALDGISHLCATAMTARDFGPPTTTPRAHFETLLKKERPVHDSSAPAATRPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
GVAFLFGSERFGMSNADVYRCHVTLSIPTQPGFGSLNLGAAVQVIAYEWRMALDGCGLAV
CEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEC
PDGTPARHLADAVQVAGMLEHWEQALTQIGFLDPAAPKKLMPRLQQLFNRAQLAPEEIHI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
LRGIAKAIIATAQANRQRPSPSSDET
HHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA