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The map label for this gene is spoT [H]

Identifier: 121611136

GI number: 121611136

Start: 4646854

End: 4649136

Strand: Direct

Name: spoT [H]

Synonym: Veis_4220

Alternate gene names: 121611136

Gene position: 4646854-4649136 (Clockwise)

Preceding gene: 121611135

Following gene: 121611139

Centisome position: 83.48

GC content: 63.21

Gene sequence:

>2283_bases
GTGGTGCCCCCCTCCATCTGCGCACCGCAACCCCGGCCTGACCCGGCGCCAGCGCAAGACGGCGCAGCCCCTGCCGCCGT
AGCCGCAGCCAATGCCGCAGCGGCCAGTTTCACCGCGCTGACCAGCCGCCTGGACTATCTGGACGCAGCGAGCATCGAAC
AGGTGCGCCAAGCCTACCGCTTTGCCGATGAGGCGCATCTGGGCCAGTTACGCGACAGCGGTGAGCCCTACATCACCCAC
CCGATCGCAGTGGCCGCCCAATGCACCGCCTGGAAGCTCGACGCCCAGGCCCTGATGGCCGCCTTGCTGCATGACGCGAT
GGAAGATTGCGACGTCACCAAAGCCAACCTGATCGACCGCTTCGGCGCACCAGTGGCCGAACTGGTCGATGGCCTGACCA
AGCTGGACCACCTGCCAATCATCACCCGCGAAGAAAACCAGGCCAAGTCCTTTCGCAAAATGCTGCTGGCCACGGCGCGC
GATGTGCGCGTCATCCTGATCAAACTGGCCGACCGCACGCACAACATGCGCACGCTGTCCGACACGCCGCGCAGCAAATG
GGCCCGCATCTCGATGGAGACGCTGGAAATCTACGCCCCCATCGCCCACCGCCTGGGGCTGGACCAGACCTACCGCGAAC
TGCAAGACCTGGCCTTCAGGCACCTGCACCCCTGGCGCCATGCCACGCTGGACAAAGCCGTCAGCAAGCTGCGCAACAGC
CGCAGGCGCGACCTGCTCCAGAAAGTCCAGACCGAAGTCAGCGCCGCGTTCACGAAAATCGGCATGAAAGTGAGCCTGAC
GGGCCGCGAAAAAACCCTGTATGCCATCTATCAGAAGATGAAACTCAAGCACCTGGGCTTCCTCCAGGTCACCGACCTGT
ACGGCTTTCGGATCATCGTTCCCACCGTGACCGACTGCTACACGGCGCTGGGAGTGCTGCACCAGATGTACCAGCCGGTG
CCCGGCAGGTTCAAGGACTACATAGCGATTGCCAAGCCCAACGGCTACCAGTCGCTGCACACCACCCTGGTCGGGCCATC
GGGGGTGAACATCGAGTTTCAGATGCGCACCGATGCAATGCACGCCATCGCCGAATCCGGCGTGGCCACGCACTGGCTCT
ACAAGTCCCAGGACACGGAAGGAATATCCGCCGAGCACCTGGACACCCACCAGTGGCTGCAATCCTTGCTCGACATTCAG
AATGAAACCCGGGGCGCGGCCGAATTCTGGGAGCATGTGAAGGTGGACCTGTTCCCCGATACCGTCTACGTCTTGACGCC
CAAGAGCCTGATCATGGCCTTGCCACGCGGAGCCACCGTGGTCGACTTTGCCTACGCCATCCACAGCAACGTAGGCGATC
ACACCACGGCCGCGAAGATCAACAACGAACAGGTTCCGCTGCGCACCGAACTCAAGAACGGCGACACGGTGCAAGTGATC
ACCGACCCGGCCTCCACGCCCAACCCCGCATGGCTGAGCTTCGTGCGCACCGGCCAGGCGCGCTCGAAGATTCGCCACTA
CCTAAAAACCTTGGCGCAGACCGAACCGCAAGGATTGGGAGAAAAACTGCTGGCGCAGGCGCTTCGGGCCGAAGGCTTGG
AACAACTGCCCCCGGTGGACGCCACGACACAGCCCCTGTGGGACAAACTGCTGCGCTTTACCGGCAACCGGACCCGCAAC
GAGCTGCTGACCGACCTGGTACTGGGCAAGCGCATCGCCAGCATCGTGGCCAAGCGGCTGATGATTTTGATGACCGAGCA
TGGACATCGCCCCGACGCATTGCTGCTCAGCCGCGAGCGCTACACCTCGCACGAGACCCTGTCCCAGGGCTCGGTGACGC
TCGACGGCAGCGAAACCGCATCAATCCAGTATGCGCCCTGCTGCCGCCCGGTGCCGGGCGACAACGTCGTCGGCTACCTC
GGTCACGGCGAAGGACTGGCGGTGCACACGGCGCACTGCGGCGTTGCCAAGCGCCTGCAACACAAGGACAGCGAGCGCTT
CATCGCGGTCGACTGGTCCGACGAGCCTACACGCATGTTCGAAACCGGCGTGGTCGTCACCGTGACCAATGGCAAAGGCG
TTCTGGCCCGGGTAGCAGGGCAACTGGCCAATTCCGAAGCCGACATCACCCATGTGGGCATGGACGATGAAGTCGCCATG
AAAACCATCGACCTGCGTTTCAGCATTGCCGTGCACGACCGAACGCATCTGGAGACCATACTGCACAACCTGCGCCACAC
CCCATCGGTCATCCGGGCGGCCCGGATGACACCGAAGGTTTGA

Upstream 100 bases:

>100_bases
GGCAGGCAAAGTCCCCCCAAAGCAGCGCCACCACAGCGGCGCTGGCCCAGGCCCCGAGATCGTCGATCCCCTCCCGCACT
GCATCACCGAAAGCCTGAAC

Downstream 100 bases:

>100_bases
TGGGGCGGGCGGGCTTGGCTGCGGCGCAGAACGACGCCAACCCGAGCGGGTCGCAATTACAGCGCCATGGCAGCGCCGAC
CGACGCCCTCCTCCCGCCCC

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MVPPSICAPQPRPDPAPAQDGAAPAAVAAANAAAASFTALTSRLDYLDAASIEQVRQAYRFADEAHLGQLRDSGEPYITH
PIAVAAQCTAWKLDAQALMAALLHDAMEDCDVTKANLIDRFGAPVAELVDGLTKLDHLPIITREENQAKSFRKMLLATAR
DVRVILIKLADRTHNMRTLSDTPRSKWARISMETLEIYAPIAHRLGLDQTYRELQDLAFRHLHPWRHATLDKAVSKLRNS
RRRDLLQKVQTEVSAAFTKIGMKVSLTGREKTLYAIYQKMKLKHLGFLQVTDLYGFRIIVPTVTDCYTALGVLHQMYQPV
PGRFKDYIAIAKPNGYQSLHTTLVGPSGVNIEFQMRTDAMHAIAESGVATHWLYKSQDTEGISAEHLDTHQWLQSLLDIQ
NETRGAAEFWEHVKVDLFPDTVYVLTPKSLIMALPRGATVVDFAYAIHSNVGDHTTAAKINNEQVPLRTELKNGDTVQVI
TDPASTPNPAWLSFVRTGQARSKIRHYLKTLAQTEPQGLGEKLLAQALRAEGLEQLPPVDATTQPLWDKLLRFTGNRTRN
ELLTDLVLGKRIASIVAKRLMILMTEHGHRPDALLLSRERYTSHETLSQGSVTLDGSETASIQYAPCCRPVPGDNVVGYL
GHGEGLAVHTAHCGVAKRLQHKDSERFIAVDWSDEPTRMFETGVVVTVTNGKGVLARVAGQLANSEADITHVGMDDEVAM
KTIDLRFSIAVHDRTHLETILHNLRHTPSVIRAARMTPKV

Sequences:

>Translated_760_residues
MVPPSICAPQPRPDPAPAQDGAAPAAVAAANAAAASFTALTSRLDYLDAASIEQVRQAYRFADEAHLGQLRDSGEPYITH
PIAVAAQCTAWKLDAQALMAALLHDAMEDCDVTKANLIDRFGAPVAELVDGLTKLDHLPIITREENQAKSFRKMLLATAR
DVRVILIKLADRTHNMRTLSDTPRSKWARISMETLEIYAPIAHRLGLDQTYRELQDLAFRHLHPWRHATLDKAVSKLRNS
RRRDLLQKVQTEVSAAFTKIGMKVSLTGREKTLYAIYQKMKLKHLGFLQVTDLYGFRIIVPTVTDCYTALGVLHQMYQPV
PGRFKDYIAIAKPNGYQSLHTTLVGPSGVNIEFQMRTDAMHAIAESGVATHWLYKSQDTEGISAEHLDTHQWLQSLLDIQ
NETRGAAEFWEHVKVDLFPDTVYVLTPKSLIMALPRGATVVDFAYAIHSNVGDHTTAAKINNEQVPLRTELKNGDTVQVI
TDPASTPNPAWLSFVRTGQARSKIRHYLKTLAQTEPQGLGEKLLAQALRAEGLEQLPPVDATTQPLWDKLLRFTGNRTRN
ELLTDLVLGKRIASIVAKRLMILMTEHGHRPDALLLSRERYTSHETLSQGSVTLDGSETASIQYAPCCRPVPGDNVVGYL
GHGEGLAVHTAHCGVAKRLQHKDSERFIAVDWSDEPTRMFETGVVVTVTNGKGVLARVAGQLANSEADITHVGMDDEVAM
KTIDLRFSIAVHDRTHLETILHNLRHTPSVIRAARMTPKV
>Mature_760_residues
MVPPSICAPQPRPDPAPAQDGAAPAAVAAANAAAASFTALTSRLDYLDAASIEQVRQAYRFADEAHLGQLRDSGEPYITH
PIAVAAQCTAWKLDAQALMAALLHDAMEDCDVTKANLIDRFGAPVAELVDGLTKLDHLPIITREENQAKSFRKMLLATAR
DVRVILIKLADRTHNMRTLSDTPRSKWARISMETLEIYAPIAHRLGLDQTYRELQDLAFRHLHPWRHATLDKAVSKLRNS
RRRDLLQKVQTEVSAAFTKIGMKVSLTGREKTLYAIYQKMKLKHLGFLQVTDLYGFRIIVPTVTDCYTALGVLHQMYQPV
PGRFKDYIAIAKPNGYQSLHTTLVGPSGVNIEFQMRTDAMHAIAESGVATHWLYKSQDTEGISAEHLDTHQWLQSLLDIQ
NETRGAAEFWEHVKVDLFPDTVYVLTPKSLIMALPRGATVVDFAYAIHSNVGDHTTAAKINNEQVPLRTELKNGDTVQVI
TDPASTPNPAWLSFVRTGQARSKIRHYLKTLAQTEPQGLGEKLLAQALRAEGLEQLPPVDATTQPLWDKLLRFTGNRTRN
ELLTDLVLGKRIASIVAKRLMILMTEHGHRPDALLLSRERYTSHETLSQGSVTLDGSETASIQYAPCCRPVPGDNVVGYL
GHGEGLAVHTAHCGVAKRLQHKDSERFIAVDWSDEPTRMFETGVVVTVTNGKGVLARVAGQLANSEADITHVGMDDEVAM
KTIDLRFSIAVHDRTHLETILHNLRHTPSVIRAARMTPKV

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=711, Percent_Identity=41.4908579465542, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1789147, Length=689, Percent_Identity=31.7851959361393, Blast_Score=343, Evalue=3e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 84216; Mature: 84216

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPPSICAPQPRPDPAPAQDGAAPAAVAAANAAAASFTALTSRLDYLDAASIEQVRQAYR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FADEAHLGQLRDSGEPYITHPIAVAAQCTAWKLDAQALMAALLHDAMEDCDVTKANLIDR
HHHHHHHHHHHCCCCCEEECCHHHHCCCHHEECCHHHHHHHHHHHHHHHCCCHHHHHHHH
FGAPVAELVDGLTKLDHLPIITREENQAKSFRKMLLATARDVRVILIKLADRTHNMRTLS
HCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
DTPRSKWARISMETLEIYAPIAHRLGLDQTYRELQDLAFRHLHPWRHATLDKAVSKLRNS
CCCCHHHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RRRDLLQKVQTEVSAAFTKIGMKVSLTGREKTLYAIYQKMKLKHLGFLQVTDLYGFRIIV
HHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
PTVTDCYTALGVLHQMYQPVPGRFKDYIAIAKPNGYQSLHTTLVGPSGVNIEFQMRTDAM
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHEEECCCCCCEEEEECHHHH
HAIAESGVATHWLYKSQDTEGISAEHLDTHQWLQSLLDIQNETRGAAEFWEHVKVDLFPD
HHHHHCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCEEEECCC
TVYVLTPKSLIMALPRGATVVDFAYAIHSNVGDHTTAAKINNEQVPLRTELKNGDTVQVI
EEEEECCHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCEEEEE
TDPASTPNPAWLSFVRTGQARSKIRHYLKTLAQTEPQGLGEKLLAQALRAEGLEQLPPVD
ECCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCC
ATTQPLWDKLLRFTGNRTRNELLTDLVLGKRIASIVAKRLMILMTEHGHRPDALLLSRER
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHH
YTSHETLSQGSVTLDGSETASIQYAPCCRPVPGDNVVGYLGHGEGLAVHTAHCGVAKRLQ
HCCHHHHCCCCEEECCCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHH
HKDSERFIAVDWSDEPTRMFETGVVVTVTNGKGVLARVAGQLANSEADITHVGMDDEVAM
CCCCCCEEEEECCCCCCHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCHHHH
KTIDLRFSIAVHDRTHLETILHNLRHTPSVIRAARMTPKV
EEEEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MVPPSICAPQPRPDPAPAQDGAAPAAVAAANAAAASFTALTSRLDYLDAASIEQVRQAYR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FADEAHLGQLRDSGEPYITHPIAVAAQCTAWKLDAQALMAALLHDAMEDCDVTKANLIDR
HHHHHHHHHHHCCCCCEEECCHHHHCCCHHEECCHHHHHHHHHHHHHHHCCCHHHHHHHH
FGAPVAELVDGLTKLDHLPIITREENQAKSFRKMLLATARDVRVILIKLADRTHNMRTLS
HCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
DTPRSKWARISMETLEIYAPIAHRLGLDQTYRELQDLAFRHLHPWRHATLDKAVSKLRNS
CCCCHHHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RRRDLLQKVQTEVSAAFTKIGMKVSLTGREKTLYAIYQKMKLKHLGFLQVTDLYGFRIIV
HHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
PTVTDCYTALGVLHQMYQPVPGRFKDYIAIAKPNGYQSLHTTLVGPSGVNIEFQMRTDAM
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHEEECCCCCCEEEEECHHHH
HAIAESGVATHWLYKSQDTEGISAEHLDTHQWLQSLLDIQNETRGAAEFWEHVKVDLFPD
HHHHHCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCEEEECCC
TVYVLTPKSLIMALPRGATVVDFAYAIHSNVGDHTTAAKINNEQVPLRTELKNGDTVQVI
EEEEECCHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCEEEEE
TDPASTPNPAWLSFVRTGQARSKIRHYLKTLAQTEPQGLGEKLLAQALRAEGLEQLPPVD
ECCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCC
ATTQPLWDKLLRFTGNRTRNELLTDLVLGKRIASIVAKRLMILMTEHGHRPDALLLSRER
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHH
YTSHETLSQGSVTLDGSETASIQYAPCCRPVPGDNVVGYLGHGEGLAVHTAHCGVAKRLQ
HCCHHHHCCCCEEECCCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHH
HKDSERFIAVDWSDEPTRMFETGVVVTVTNGKGVLARVAGQLANSEADITHVGMDDEVAM
CCCCCCEEEEECCCCCCHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCHHHH
KTIDLRFSIAVHDRTHLETILHNLRHTPSVIRAARMTPKV
EEEEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]