Definition Verminephrobacter eiseniae EF01-2 chromosome, complete genome.
Accession NC_008786
Length 5,566,749

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The map label for this gene is braF [H]

Identifier: 121611026

GI number: 121611026

Start: 4509081

End: 4509800

Strand: Direct

Name: braF [H]

Synonym: Veis_4110

Alternate gene names: 121611026

Gene position: 4509081-4509800 (Clockwise)

Preceding gene: 121611025

Following gene: 121611027

Centisome position: 81.0

GC content: 67.5

Gene sequence:

>720_bases
ATGACGCCCTATGTGTTGCAAGCCGAGGATGTGGCCATCCATTACGGCGGCACCAAGGCCCTCGACGGTGTTGCGCTGAC
CTTGGAGAAAGCCCAGATTCGCGGGCTGATCGGCCCCAACGGCGCCGGAAAATCCACGCTCATCGATGCCATCACCGGCC
GGCGCCGCCTGGCCCGGGGCAAGGTCATCCTCCACGGCGAGGATGTCAGTCGGCTGGGCACCGTCGAGCGCCGCCAGCGG
GGGCTGTCGCGCAGCTTTCAACGGACCAGCATCTTTGCCGGCATGACCGTCCTCGCGCAGGTGGAACTGGCGTCGCACGA
GATGGGCGTCGAAAACTCTGCCGCCGATGCGCGCGCGGTGCTGGAAGAATTGAACCTCGCCAGCCTGGGCCACAGGCTGG
CCGGCAACCTGGGCTACGGCGAGCAGCGCCGCCTGGACCTGGCGCTGGCCCTGGTCGGACGCCCCAAGCTGCTGATGCTC
GACGAGCCCATGGCCGGGCTTTCCGTCAAGGAGTCGCTGGACCTGGCGCAGCACCTGAAGGCCCTGGCCTCGCGCCGGGA
CATCGCCGTATTGCTGGTGGAACACGACATGGACGTGGTCTTTGGCATCTGCAGCGTCATCACCGTCTTCGAACTGGGCC
GGGTCATCGCCGATGGCGCACCGGCCAGCGTGCGCGCCAACCGGCGGGTGCGCGAGGCCTATCTGGGAAGCGCAGCATGA

Upstream 100 bases:

>100_bases
TGATTGCCGTCATCGTGTTCTCGCCGCACGGCATCGCCGGCGCGCTGGAGCGTCTGTACATTCGCTTGCGCCATGGCCCC
GGCGTCCGCAGGGAATCGGT

Downstream 100 bases:

>100_bases
GCGCCGCGCGGCCGCCCGAAGGCGAAGGTACTCCAGTGAGGAGCGCGCTGCTGACACTGACCGGCGTGGATGCGTTCTAC
GGTTCGGCCCATATCCTGCA

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTPYVLQAEDVAIHYGGTKALDGVALTLEKAQIRGLIGPNGAGKSTLIDAITGRRRLARGKVILHGEDVSRLGTVERRQR
GLSRSFQRTSIFAGMTVLAQVELASHEMGVENSAADARAVLEELNLASLGHRLAGNLGYGEQRRLDLALALVGRPKLLML
DEPMAGLSVKESLDLAQHLKALASRRDIAVLLVEHDMDVVFGICSVITVFELGRVIADGAPASVRANRRVREAYLGSAA

Sequences:

>Translated_239_residues
MTPYVLQAEDVAIHYGGTKALDGVALTLEKAQIRGLIGPNGAGKSTLIDAITGRRRLARGKVILHGEDVSRLGTVERRQR
GLSRSFQRTSIFAGMTVLAQVELASHEMGVENSAADARAVLEELNLASLGHRLAGNLGYGEQRRLDLALALVGRPKLLML
DEPMAGLSVKESLDLAQHLKALASRRDIAVLLVEHDMDVVFGICSVITVFELGRVIADGAPASVRANRRVREAYLGSAA
>Mature_238_residues
TPYVLQAEDVAIHYGGTKALDGVALTLEKAQIRGLIGPNGAGKSTLIDAITGRRRLARGKVILHGEDVSRLGTVERRQRG
LSRSFQRTSIFAGMTVLAQVELASHEMGVENSAADARAVLEELNLASLGHRLAGNLGYGEQRRLDLALALVGRPKLLMLD
EPMAGLSVKESLDLAQHLKALASRRDIAVLLVEHDMDVVFGICSVITVFELGRVIADGAPASVRANRRVREAYLGSAA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI11967969, Length=218, Percent_Identity=31.1926605504587, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI150417984, Length=209, Percent_Identity=31.1004784688995, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI153792144, Length=187, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI27477115, Length=151, Percent_Identity=31.1258278145695, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI105990541, Length=209, Percent_Identity=28.2296650717703, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI47078218, Length=202, Percent_Identity=27.2277227722772, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45446740, Length=202, Percent_Identity=27.2277227722772, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI27262624, Length=196, Percent_Identity=26.0204081632653, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27262626, Length=196, Percent_Identity=26.0204081632653, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27436953, Length=188, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI27881501, Length=221, Percent_Identity=27.1493212669683, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI30795238, Length=221, Percent_Identity=27.1493212669683, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI21536376, Length=198, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI6005701, Length=207, Percent_Identity=25.6038647342995, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI31657092, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI116734710, Length=211, Percent_Identity=26.0663507109005, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1789864, Length=255, Percent_Identity=34.9019607843137, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI87081709, Length=233, Percent_Identity=26.6094420600858, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1789593, Length=236, Percent_Identity=30.9322033898305, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787712, Length=220, Percent_Identity=29.0909090909091, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1786398, Length=220, Percent_Identity=32.2727272727273, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787370, Length=213, Percent_Identity=29.1079812206573, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1788761, Length=216, Percent_Identity=30.0925925925926, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081791, Length=241, Percent_Identity=27.8008298755187, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790190, Length=226, Percent_Identity=27.4336283185841, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI48994943, Length=237, Percent_Identity=28.6919831223629, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082268, Length=240, Percent_Identity=27.9166666666667, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789672, Length=228, Percent_Identity=26.7543859649123, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787029, Length=228, Percent_Identity=27.1929824561404, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081782, Length=225, Percent_Identity=27.1111111111111, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1789991, Length=224, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788472, Length=219, Percent_Identity=26.027397260274, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788897, Length=215, Percent_Identity=32.5581395348837, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788210, Length=225, Percent_Identity=28.8888888888889, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48995001, Length=223, Percent_Identity=30.0448430493274, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787759, Length=249, Percent_Identity=30.9236947791165, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786345, Length=221, Percent_Identity=27.1493212669683, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786319, Length=207, Percent_Identity=31.8840579710145, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790739, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789032, Length=239, Percent_Identity=24.6861924686192, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1789873, Length=205, Percent_Identity=31.219512195122, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787089, Length=222, Percent_Identity=26.1261261261261, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786563, Length=196, Percent_Identity=29.0816326530612, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786253, Length=214, Percent_Identity=26.6355140186916, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788165, Length=216, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788506, Length=219, Percent_Identity=27.8538812785388, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787792, Length=230, Percent_Identity=25.6521739130435, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786698, Length=209, Percent_Identity=28.2296650717703, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790467, Length=218, Percent_Identity=25.2293577981651, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789586, Length=239, Percent_Identity=28.4518828451883, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790525, Length=244, Percent_Identity=25.8196721311475, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789963, Length=234, Percent_Identity=27.3504273504274, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081834, Length=209, Percent_Identity=27.7511961722488, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786803, Length=234, Percent_Identity=22.6495726495727, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082267, Length=211, Percent_Identity=25.1184834123223, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790162, Length=233, Percent_Identity=27.0386266094421, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788450, Length=226, Percent_Identity=25.6637168141593, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786703, Length=185, Percent_Identity=31.3513513513514, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI48994997, Length=231, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789891, Length=204, Percent_Identity=28.921568627451, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790544, Length=252, Percent_Identity=23.4126984126984, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1787500, Length=218, Percent_Identity=27.5229357798165, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786872, Length=233, Percent_Identity=24.0343347639485, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367384, Length=167, Percent_Identity=31.7365269461078, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788225, Length=233, Percent_Identity=23.6051502145923, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788644, Length=247, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994883, Length=234, Percent_Identity=25.6410256410256, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787164, Length=229, Percent_Identity=25.764192139738, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788053, Length=207, Percent_Identity=28.5024154589372, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786981, Length=198, Percent_Identity=30.8080808080808, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789962, Length=250, Percent_Identity=24.8, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=221, Percent_Identity=26.2443438914027, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71983516, Length=221, Percent_Identity=26.2443438914027, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71983510, Length=221, Percent_Identity=26.2443438914027, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17510237, Length=213, Percent_Identity=30.5164319248826, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115533608, Length=189, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17565586, Length=209, Percent_Identity=30.1435406698565, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988343, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007184, Length=215, Percent_Identity=29.7674418604651, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221500365, Length=215, Percent_Identity=29.7674418604651, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221512771, Length=233, Percent_Identity=28.755364806867, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24666092, Length=197, Percent_Identity=28.9340101522843, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24581191, Length=204, Percent_Identity=24.5098039215686, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24581189, Length=204, Percent_Identity=24.5098039215686, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24581193, Length=204, Percent_Identity=24.5098039215686, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24581195, Length=204, Percent_Identity=24.5098039215686, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI85725262, Length=218, Percent_Identity=29.8165137614679, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28574150, Length=207, Percent_Identity=29.4685990338164, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI116007328, Length=207, Percent_Identity=29.4685990338164, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24662800, Length=210, Percent_Identity=27.1428571428571, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24650853, Length=213, Percent_Identity=26.7605633802817, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281362751, Length=195, Percent_Identity=28.7179487179487, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24650855, Length=213, Percent_Identity=26.7605633802817, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28574533, Length=223, Percent_Identity=26.0089686098655, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571871, Length=230, Percent_Identity=27.8260869565217, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161076682, Length=178, Percent_Identity=30.3370786516854, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161076684, Length=178, Percent_Identity=30.3370786516854, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581617, Length=178, Percent_Identity=30.3370786516854, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581615, Length=178, Percent_Identity=30.3370786516854, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571209, Length=207, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24643674, Length=198, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24582014, Length=191, Percent_Identity=27.2251308900524, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582012, Length=191, Percent_Identity=27.2251308900524, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581387, Length=213, Percent_Identity=24.8826291079812, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581383, Length=213, Percent_Identity=24.8826291079812, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581385, Length=213, Percent_Identity=24.8826291079812, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25609; Mature: 25478

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MTPYVLQAEDVAIHYGGTKALDGVALTLEKAQIRGLIGPNGAGKSTLIDAITGRRRLARG
CCCCEEEECCEEEEECCCHHHCCEEEEEEHHHHCEEECCCCCCHHHHHHHHHHHHHHHCC
KVILHGEDVSRLGTVERRQRGLSRSFQRTSIFAGMTVLAQVELASHEMGVENSAADARAV
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LEELNLASLGHRLAGNLGYGEQRRLDLALALVGRPKLLMLDEPMAGLSVKESLDLAQHLK
HHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHH
ALASRRDIAVLLVEHDMDVVFGICSVITVFELGRVIADGAPASVRANRRVREAYLGSAA
HHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TPYVLQAEDVAIHYGGTKALDGVALTLEKAQIRGLIGPNGAGKSTLIDAITGRRRLARG
CCCEEEECCEEEEECCCHHHCCEEEEEEHHHHCEEECCCCCCHHHHHHHHHHHHHHHCC
KVILHGEDVSRLGTVERRQRGLSRSFQRTSIFAGMTVLAQVELASHEMGVENSAADARAV
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LEELNLASLGHRLAGNLGYGEQRRLDLALALVGRPKLLMLDEPMAGLSVKESLDLAQHLK
HHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHH
ALASRRDIAVLLVEHDMDVVFGICSVITVFELGRVIADGAPASVRANRRVREAYLGSAA
HHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]