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The map label for this gene is braC [H]

Identifier: 121611016

GI number: 121611016

Start: 4497924

End: 4499087

Strand: Direct

Name: braC [H]

Synonym: Veis_4100

Alternate gene names: 121611016

Gene position: 4497924-4499087 (Clockwise)

Preceding gene: 121611015

Following gene: 121611017

Centisome position: 80.8

GC content: 62.54

Gene sequence:

>1164_bases
ATGAAACTTCATCACTTTGCCGCATCGATCATTGCCAGCCTGGCCATGGCCGGCGCCTGTGCGCAGACCCAGGGCGTCGC
CAAGGACAGCATCACGCTGGGCTCGATACAAGACCTCTCCGGCCCGCTGGCCGGCTTTGGCAAGCAGGCGCGGCTGGGGA
TGATGCTGCGCGTCGACGAGGCCAACGAACAGGGCGGCGTCAACGGCCGCAAGCTCGATCTGAAGGTCGAGGACTCGGGC
TACGACCCCAAGAGGGCGGTGCTGGCGGCGCAAAAGCTGGTCAACCAGGACAAAATCTTCATCATGATCGGGCATATCGG
CACGGCCCAGAACCTGGCCGCAATGCCGGTGCAGTTCAGCAAGAACGTCATCAACTTTCTGCCCCTGACCGGCGCGCGCG
AGATGTATGAACCCTGGCACAGGCTCAAGTATTCGTCTTTCGTCCCCTACTACGACCAGATTCGCCTGGCAGTGCCCCGG
CTGATCAAGGAAAAGAACGCCCGCAAAATCTGCACCATCTACCAGGACGACGAGTTCGGCCTGGAGGTGATGCGCGGCGG
CGAGGCGGCCCTCAAGACCCTGGGCCTGGAGTTCACCGAAAAGACCACGTACAAGCGCGGCGCCACCGATTTCTCGTCCC
AGGTGGCGAAGATGAAATCCGCCGGCTGCGACCTCGTGATCCTGGGCACCGTGATCCGCGAAACCATAGGCACCATCGGC
GAGGCGCGCAAAACCGGCTTCGACCCCACCTTCCTGGGCTCCGGCGCCGCCTACACCGACCTGATCCACAAACTCGGCGG
CAAGCCCATGGACGGCCTGTACGCCGCGATGACCGTGCAGCACCCCTATCTGGATGAAGCCTCGCAGCCGCTGCGCTTTT
GGGCCAACAAGTACAAGACCCGGTTCAACGAAGATCCGAGCGTGTTCTCGGTCTATGGCTACAGCGTGGTCGATGTGTTC
ATTCGCGCAGCCCAAAAAGCCGGCCCCCAACTGACGACCGACAGCTTCATCGCGGCCATGGACACCATGCTCATCGCGCC
CGACATCTTCGGCAACCCCGAGGTCAGCTACGGCCCGCAAAAGCGCCTGGGCAGCGACCTGACGCGCCTGTCGCAAATCG
TCGACGGCAAGTGGAAGGTGGTGTCCGACTACATCCAGCCCTGA

Upstream 100 bases:

>100_bases
CAGATCGAGGCCATGTACCGCTAGGAAAAATGCTGCCGCCCCGCCCCGCCGCCCCGGGCATCGTCCCGTCACCACTGATC
CACTGCAGGAGAAATCGACC

Downstream 100 bases:

>100_bases
ACCGCCGCGAGGTCTGGCGGCAGCCCCCGAGCCAACGGCTTTTCATGCATCAGCCTGCCCGGGAAATACGGCGCCCCCGG
GGCCAACCAACCCGGTTCCT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKLHHFAASIIASLAMAGACAQTQGVAKDSITLGSIQDLSGPLAGFGKQARLGMMLRVDEANEQGGVNGRKLDLKVEDSG
YDPKRAVLAAQKLVNQDKIFIMIGHIGTAQNLAAMPVQFSKNVINFLPLTGAREMYEPWHRLKYSSFVPYYDQIRLAVPR
LIKEKNARKICTIYQDDEFGLEVMRGGEAALKTLGLEFTEKTTYKRGATDFSSQVAKMKSAGCDLVILGTVIRETIGTIG
EARKTGFDPTFLGSGAAYTDLIHKLGGKPMDGLYAAMTVQHPYLDEASQPLRFWANKYKTRFNEDPSVFSVYGYSVVDVF
IRAAQKAGPQLTTDSFIAAMDTMLIAPDIFGNPEVSYGPQKRLGSDLTRLSQIVDGKWKVVSDYIQP

Sequences:

>Translated_387_residues
MKLHHFAASIIASLAMAGACAQTQGVAKDSITLGSIQDLSGPLAGFGKQARLGMMLRVDEANEQGGVNGRKLDLKVEDSG
YDPKRAVLAAQKLVNQDKIFIMIGHIGTAQNLAAMPVQFSKNVINFLPLTGAREMYEPWHRLKYSSFVPYYDQIRLAVPR
LIKEKNARKICTIYQDDEFGLEVMRGGEAALKTLGLEFTEKTTYKRGATDFSSQVAKMKSAGCDLVILGTVIRETIGTIG
EARKTGFDPTFLGSGAAYTDLIHKLGGKPMDGLYAAMTVQHPYLDEASQPLRFWANKYKTRFNEDPSVFSVYGYSVVDVF
IRAAQKAGPQLTTDSFIAAMDTMLIAPDIFGNPEVSYGPQKRLGSDLTRLSQIVDGKWKVVSDYIQP
>Mature_387_residues
MKLHHFAASIIASLAMAGACAQTQGVAKDSITLGSIQDLSGPLAGFGKQARLGMMLRVDEANEQGGVNGRKLDLKVEDSG
YDPKRAVLAAQKLVNQDKIFIMIGHIGTAQNLAAMPVQFSKNVINFLPLTGAREMYEPWHRLKYSSFVPYYDQIRLAVPR
LIKEKNARKICTIYQDDEFGLEVMRGGEAALKTLGLEFTEKTTYKRGATDFSSQVAKMKSAGCDLVILGTVIRETIGTIG
EARKTGFDPTFLGSGAAYTDLIHKLGGKPMDGLYAAMTVQHPYLDEASQPLRFWANKYKTRFNEDPSVFSVYGYSVVDVF
IRAAQKAGPQLTTDSFIAAMDTMLIAPDIFGNPEVSYGPQKRLGSDLTRLSQIVDGKWKVVSDYIQP

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=245, Percent_Identity=25.3061224489796, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789867, Length=275, Percent_Identity=23.2727272727273, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42471; Mature: 42471

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHHFAASIIASLAMAGACAQTQGVAKDSITLGSIQDLSGPLAGFGKQARLGMMLRVDE
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHCCCHHCCCCCHHCCEEEEEEC
ANEQGGVNGRKLDLKVEDSGYDPKRAVLAAQKLVNQDKIFIMIGHIGTAQNLAAMPVQFS
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCHHHH
KNVINFLPLTGAREMYEPWHRLKYSSFVPYYDQIRLAVPRLIKEKNARKICTIYQDDEFG
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
LEVMRGGEAALKTLGLEFTEKTTYKRGATDFSSQVAKMKSAGCDLVILGTVIRETIGTIG
HHHHCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
EARKTGFDPTFLGSGAAYTDLIHKLGGKPMDGLYAAMTVQHPYLDEASQPLRFWANKYKT
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
RFNEDPSVFSVYGYSVVDVFIRAAQKAGPQLTTDSFIAAMDTMLIAPDIFGNPEVSYGPQ
HCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCH
KRLGSDLTRLSQIVDGKWKVVSDYIQP
HHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MKLHHFAASIIASLAMAGACAQTQGVAKDSITLGSIQDLSGPLAGFGKQARLGMMLRVDE
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHCCCHHCCCCCHHCCEEEEEEC
ANEQGGVNGRKLDLKVEDSGYDPKRAVLAAQKLVNQDKIFIMIGHIGTAQNLAAMPVQFS
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCHHHH
KNVINFLPLTGAREMYEPWHRLKYSSFVPYYDQIRLAVPRLIKEKNARKICTIYQDDEFG
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
LEVMRGGEAALKTLGLEFTEKTTYKRGATDFSSQVAKMKSAGCDLVILGTVIRETIGTIG
HHHHCCCHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
EARKTGFDPTFLGSGAAYTDLIHKLGGKPMDGLYAAMTVQHPYLDEASQPLRFWANKYKT
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
RFNEDPSVFSVYGYSVVDVFIRAAQKAGPQLTTDSFIAAMDTMLIAPDIFGNPEVSYGPQ
HCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCH
KRLGSDLTRLSQIVDGKWKVVSDYIQP
HHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]