BacMap

Definition	Verminephrobacter eiseniae EF01-2 chromosome, complete genome.
Accession	NC_008786
Length	5,566,749

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The map label for this gene is braG [H]

Identifier: 121610990

GI number: 121610990

Start: 4466748

End: 4467473

Strand: Direct

Name: braG [H]

Synonym: Veis_4074

Alternate gene names: 121610990

Gene position: 4466748-4467473 (Clockwise)

Preceding gene: 121610989

Following gene: 121610991

Centisome position: 80.24

GC content: 65.01

Gene sequence:

>726_bases
ATGGCGGCCCCTGCAGCGAACACCCCGGCGCTCGAAATCTGCAACCTTCAGGCTTGGTATGGCCAGTCGCATGTGCTGCA
TGGCGTGGACATCGTCGTGCGGCCCGGTGAAGTGGTCACGCTGCTCGGGCGCAATGGCGCGGGCCGCACCACCACGCTGC
GCGCCATCATGGGTTTGACCGGAACGCGCCAGGGCACGATCAGGATCAACGGCGTCGAGACGGTGAACCTGCCCACGCAC
CGCATTGCCCACCTGGGCGTGGGCTACTGCCCGGAAGAGCGCGGCATCTTCTCCAGCCTGTCGTGTCAAGAAAATCTGCT
GTTGCCCCCGCTGCTCAAAACCGGCTCGGCGGGCATGTCGGTGCAGGAAATCTACGCCCTGTTCCCTAACCTGGCCGAGC
GCAAGAACAGCCAGGGCACGCGGCTGTCCGGTGGCGAGCAGCAGATGCTGGCCGTGGCGCGCATTTTGCGCACCGGCGCC
CGGTTGCTGCTGCTCGATGAAATCTCCGAGGGGCTGGCCCCGGTCATCGTTCAGGCCCTGGCGCGCATGATCACCATGCT
GCGCGAAAAGGGCTACACCGTGGTCATGGTCGAGCAGAACTTCCACTTTGCCGCGCCGCTGGCCGACCGTTTTTACGTGA
TGGAGCATGGCCGCATCGTCGAGCAGTTCGGCGCCCGCGAGCTCCCGCAGAAAATGCCCGTGCTCACTGAACTGCTCGGC
GTTTGA

Upstream 100 bases:

>100_bases
GTGCTGGCCGAAGGGCCTTACGCCGAAGTGTCGAACAACCCGCAGGTGATGCAGGCCTACATGGGCACGACCGACGGCCC
ATTGCAGGGGGCGCACTGAC

Downstream 100 bases:

>100_bases
GGCCCGCCCTGCCGCCCCGCATCTCCCCCCTCCCCCGCTTACCCCGCTTATCCCAATCCCCGGTTTTCATCCACAGGAGA
AGATCCATGAAGAAACGATC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MAAPAANTPALEICNLQAWYGQSHVLHGVDIVVRPGEVVTLLGRNGAGRTTTLRAIMGLTGTRQGTIRINGVETVNLPTH
RIAHLGVGYCPEERGIFSSLSCQENLLLPPLLKTGSAGMSVQEIYALFPNLAERKNSQGTRLSGGEQQMLAVARILRTGA
RLLLLDEISEGLAPVIVQALARMITMLREKGYTVVMVEQNFHFAAPLADRFYVMEHGRIVEQFGARELPQKMPVLTELLG
V

Sequences:

>Translated_241_residues
MAAPAANTPALEICNLQAWYGQSHVLHGVDIVVRPGEVVTLLGRNGAGRTTTLRAIMGLTGTRQGTIRINGVETVNLPTH
RIAHLGVGYCPEERGIFSSLSCQENLLLPPLLKTGSAGMSVQEIYALFPNLAERKNSQGTRLSGGEQQMLAVARILRTGA
RLLLLDEISEGLAPVIVQALARMITMLREKGYTVVMVEQNFHFAAPLADRFYVMEHGRIVEQFGARELPQKMPVLTELLG
V
>Mature_240_residues
AAPAANTPALEICNLQAWYGQSHVLHGVDIVVRPGEVVTLLGRNGAGRTTTLRAIMGLTGTRQGTIRINGVETVNLPTHR
IAHLGVGYCPEERGIFSSLSCQENLLLPPLLKTGSAGMSVQEIYALFPNLAERKNSQGTRLSGGEQQMLAVARILRTGAR
LLLLDEISEGLAPVIVQALARMITMLREKGYTVVMVEQNFHFAAPLADRFYVMEHGRIVEQFGARELPQKMPVLTELLGV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=224, Percent_Identity=25.8928571428571, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI21536376, Length=219, Percent_Identity=27.3972602739726, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI105990541, Length=195, Percent_Identity=28.7179487179487, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI27436953, Length=195, Percent_Identity=28.7179487179487, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI150417984, Length=222, Percent_Identity=29.2792792792793, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI45446740, Length=196, Percent_Identity=27.5510204081633, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI47078218, Length=196, Percent_Identity=27.5510204081633, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27881501, Length=203, Percent_Identity=28.0788177339901, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI30795238, Length=203, Percent_Identity=28.0788177339901, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI116734710, Length=186, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI9961250, Length=223, Percent_Identity=26.9058295964126, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4505771, Length=223, Percent_Identity=26.9058295964126, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI9961252, Length=223, Percent_Identity=26.9058295964126, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI255708477, Length=217, Percent_Identity=27.6497695852535, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI148612844, Length=229, Percent_Identity=26.6375545851528, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI153792144, Length=203, Percent_Identity=25.1231527093596, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI27262624, Length=219, Percent_Identity=25.5707762557078, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27262626, Length=219, Percent_Identity=25.5707762557078, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27477115, Length=224, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87082268, Length=211, Percent_Identity=40.7582938388626, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788225, Length=231, Percent_Identity=35.0649350649351, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789672, Length=232, Percent_Identity=32.3275862068966, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787029, Length=218, Percent_Identity=31.651376146789, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789593, Length=230, Percent_Identity=31.7391304347826, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790525, Length=222, Percent_Identity=27.4774774774775, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788450, Length=219, Percent_Identity=31.5068493150685, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788761, Length=234, Percent_Identity=30.3418803418803, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787792, Length=221, Percent_Identity=33.0316742081448, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789864, Length=227, Percent_Identity=27.3127753303965, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787370, Length=231, Percent_Identity=29.4372294372294, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788897, Length=212, Percent_Identity=30.6603773584906, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787089, Length=227, Percent_Identity=29.9559471365639, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48995001, Length=220, Percent_Identity=31.3636363636364, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1786872, Length=213, Percent_Identity=31.924882629108, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87081791, Length=222, Percent_Identity=29.7297297297297, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787712, Length=228, Percent_Identity=28.5087719298246, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788165, Length=215, Percent_Identity=29.7674418604651, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786703, Length=229, Percent_Identity=30.5676855895196, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787105, Length=223, Percent_Identity=27.3542600896861, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788644, Length=237, Percent_Identity=28.6919831223629, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786253, Length=231, Percent_Identity=29.8701298701299, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786319, Length=241, Percent_Identity=26.5560165975104, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790190, Length=218, Percent_Identity=24.3119266055046, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786398, Length=209, Percent_Identity=24.8803827751196, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790162, Length=235, Percent_Identity=27.2340425531915, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787112, Length=228, Percent_Identity=32.0175438596491, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789991, Length=238, Percent_Identity=26.890756302521, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87081782, Length=206, Percent_Identity=27.6699029126214, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789873, Length=207, Percent_Identity=23.6714975845411, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788472, Length=255, Percent_Identity=23.921568627451, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786698, Length=199, Percent_Identity=27.6381909547739, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788210, Length=227, Percent_Identity=24.6696035242291, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87081709, Length=216, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081834, Length=227, Percent_Identity=27.3127753303965, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790467, Length=215, Percent_Identity=26.5116279069767, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI48994943, Length=227, Percent_Identity=24.2290748898678, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790739, Length=223, Percent_Identity=26.9058295964126, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=210, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71983516, Length=207, Percent_Identity=29.4685990338164, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71983510, Length=207, Percent_Identity=29.4685990338164, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71983523, Length=207, Percent_Identity=29.4685990338164, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI115533608, Length=235, Percent_Identity=28.5106382978723, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=214, Percent_Identity=25.7009345794392, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71996809, Length=230, Percent_Identity=26.5217391304348, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193211015, Length=230, Percent_Identity=26.5217391304348, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193211017, Length=230, Percent_Identity=26.5217391304348, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17565938, Length=217, Percent_Identity=26.7281105990783, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71996550, Length=232, Percent_Identity=25, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71992285, Length=210, Percent_Identity=24.7619047619048, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115535101, Length=217, Percent_Identity=27.6497695852535, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=219, Percent_Identity=25.5707762557078, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71984940, Length=205, Percent_Identity=25.8536585365854, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71988343, Length=225, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323959, Length=212, Percent_Identity=24.5283018867925, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323217, Length=217, Percent_Identity=22.5806451612903, Blast_Score=62, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24666092, Length=218, Percent_Identity=29.8165137614679, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648314, Length=214, Percent_Identity=28.9719626168224, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI221512771, Length=218, Percent_Identity=26.605504587156, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24643674, Length=221, Percent_Identity=28.5067873303167, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574150, Length=221, Percent_Identity=28.5067873303167, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI116007328, Length=221, Percent_Identity=28.5067873303167, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28573571, Length=212, Percent_Identity=25.4716981132075, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28573573, Length=212, Percent_Identity=25.4716981132075, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI85725262, Length=192, Percent_Identity=27.6041666666667, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24641565, Length=230, Percent_Identity=24.3478260869565, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19920532, Length=212, Percent_Identity=27.3584905660377, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24580930, Length=212, Percent_Identity=27.3584905660377, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI85816269, Length=222, Percent_Identity=26.1261261261261, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24580553, Length=222, Percent_Identity=26.1261261261261, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI85724864, Length=222, Percent_Identity=26.1261261261261, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24580555, Length=222, Percent_Identity=26.1261261261261, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26220; Mature: 26089

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPAANTPALEICNLQAWYGQSHVLHGVDIVVRPGEVVTLLGRNGAGRTTTLRAIMGLT
CCCCCCCCCCEEEECCHHHCCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHCC
GTRQGTIRINGVETVNLPTHRIAHLGVGYCPEERGIFSSLSCQENLLLPPLLKTGSAGMS
CCCCCEEEECCEEEECCCHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCHHHCCCCCCH
VQEIYALFPNLAERKNSQGTRLSGGEQQMLAVARILRTGARLLLLDEISEGLAPVIVQAL
HHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHH
ARMITMLREKGYTVVMVEQNFHFAAPLADRFYVMEHGRIVEQFGARELPQKMPVLTELLG
HHHHHHHHHCCCEEEEEECCCEEECCHHHHEEEHHCCHHHHHHCHHHCHHHCHHHHHHHC
V
C
>Mature Secondary Structure 
AAPAANTPALEICNLQAWYGQSHVLHGVDIVVRPGEVVTLLGRNGAGRTTTLRAIMGLT
CCCCCCCCCEEEECCHHHCCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHCC
GTRQGTIRINGVETVNLPTHRIAHLGVGYCPEERGIFSSLSCQENLLLPPLLKTGSAGMS
CCCCCEEEECCEEEECCCHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCHHHCCCCCCH
VQEIYALFPNLAERKNSQGTRLSGGEQQMLAVARILRTGARLLLLDEISEGLAPVIVQAL
HHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHH
ARMITMLREKGYTVVMVEQNFHFAAPLADRFYVMEHGRIVEQFGARELPQKMPVLTELLG
HHHHHHHHHCCCEEEEEECCCEEECCHHHHEEEHHCCHHHHHHCHHHCHHHCHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]