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The map label for this gene is anfA [H]

Identifier: 121610908

GI number: 121610908

Start: 4378607

End: 4379584

Strand: Direct

Name: anfA [H]

Synonym: Veis_3990

Alternate gene names: 121610908

Gene position: 4378607-4379584 (Clockwise)

Preceding gene: 121610907

Following gene: 121610915

Centisome position: 78.66

GC content: 67.79

Gene sequence:

>978_bases
ATGACCGCACCTGTCGCGCTTGCCGAGATCATCGTCGGCGACCACCCTTCCATCGTCGCCTTGCGCGACCTGGTCCTGCG
CGTCGCGCGCAGCCACGCCAGCACGGTGCTCCTCTACGGCGAGACGGGCACGGGCAAGGGCTTGGTGGCGCGCGCCCTGC
ACGAGCATTCGTCCCGGGCCGACAAGGGCTTCACCGAGATCAATTGCGCGGCCATTCCCGGCAATTTGCTGGAGTCGGAG
CTTTTCGGCCATGAGCGCGGGGCGTTCACGGGCGCCGTCGCGCGCAAGGTGGGTCTGCTGGAGACCGCCCACGGCGGCAG
CGTCTTCCTGGACGAGGTGCGCGAGCTCGATCCCATGATGCAGGCCAAGATCCTGACGCTGCTGGACAGCCGGCGCTTTC
GTCGCATCGGGGCGGTGCGCGAAGTCTGCACCGATGTGCGCTTCATCGCCGCCACCAACAAGATTCTGCTGGGCGAAGTC
AACGCCGGCAAGTTCCGCGACGATCTCTACTACCGCCTGCAGGTGGTCTCGATCAACCTGCCGCCGCTGCGTGAACGCGG
CGACGACATCTTCGTGCTGGCCGAGCGGTTCCTGGCGCGCTTCAACCGCCTGCACGCCAGCCATTTCGAGCGCCTCGACG
CGCCTGTGGAAGCGGCGTTCCGGCGCTATGCCTGGCCGGGCAATGTGCGCGAGTTGGGCAACCTGCTGGAACGCATTTGC
ATCCTGGAAAGCGGCACCAGGGTGGGCTTGGAACATCTGCCGGCGCGCGTCGTGCGCGGCCCCGCGCCGGGTGTCGACGT
GGCCGAAGGCAACTACGCCGCACGCACCGCAACCTTCCAGCGCGGCATGATCGAATCTGCCTTGGCCGCCACGGGCGGCA
ACCTCGGCGCGGCGGCGAGCACGCTTGGCTTGTCGCGCCACGCGCTCCGGCACCAGATGAGCAAACTGGATATGGTGCAG
GGCGTCGGCCGTTCCTGA

Upstream 100 bases:

>100_bases
TCGGTGACGCGACACGAGGGCCGCGCCAAGTATGCCGGCATAAAGGCAGGCACCCCATTGCCGGGATAATGCCGGGCACC
CCTGTTTTTGTCCGGCGTCC

Downstream 100 bases:

>100_bases
GAGCACGCCGGGCTTGCAGCGTCGCGCATCATTCGATGCCGATGCGGTGGCTTTGCGCCACCGCGCGCCATTGCGCATAG
TCGTCGGCTACGCGGCGGCC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTAPVALAEIIVGDHPSIVALRDLVLRVARSHASTVLLYGETGTGKGLVARALHEHSSRADKGFTEINCAAIPGNLLESE
LFGHERGAFTGAVARKVGLLETAHGGSVFLDEVRELDPMMQAKILTLLDSRRFRRIGAVREVCTDVRFIAATNKILLGEV
NAGKFRDDLYYRLQVVSINLPPLRERGDDIFVLAERFLARFNRLHASHFERLDAPVEAAFRRYAWPGNVRELGNLLERIC
ILESGTRVGLEHLPARVVRGPAPGVDVAEGNYAARTATFQRGMIESALAATGGNLGAAASTLGLSRHALRHQMSKLDMVQ
GVGRS

Sequences:

>Translated_325_residues
MTAPVALAEIIVGDHPSIVALRDLVLRVARSHASTVLLYGETGTGKGLVARALHEHSSRADKGFTEINCAAIPGNLLESE
LFGHERGAFTGAVARKVGLLETAHGGSVFLDEVRELDPMMQAKILTLLDSRRFRRIGAVREVCTDVRFIAATNKILLGEV
NAGKFRDDLYYRLQVVSINLPPLRERGDDIFVLAERFLARFNRLHASHFERLDAPVEAAFRRYAWPGNVRELGNLLERIC
ILESGTRVGLEHLPARVVRGPAPGVDVAEGNYAARTATFQRGMIESALAATGGNLGAAASTLGLSRHALRHQMSKLDMVQ
GVGRS
>Mature_324_residues
TAPVALAEIIVGDHPSIVALRDLVLRVARSHASTVLLYGETGTGKGLVARALHEHSSRADKGFTEINCAAIPGNLLESEL
FGHERGAFTGAVARKVGLLETAHGGSVFLDEVRELDPMMQAKILTLLDSRRFRRIGAVREVCTDVRFIAATNKILLGEVN
AGKFRDDLYYRLQVVSINLPPLRERGDDIFVLAERFLARFNRLHASHFERLDAPVEAAFRRYAWPGNVRELGNLLERICI
LESGTRVGLEHLPARVVRGPAPGVDVAEGNYAARTATFQRGMIESALAATGGNLGAAASTLGLSRHALRHQMSKLDMVQG
VGRS

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=305, Percent_Identity=42.2950819672131, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1788550, Length=310, Percent_Identity=40.3225806451613, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1789087, Length=318, Percent_Identity=41.8238993710692, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1790299, Length=330, Percent_Identity=38.4848484848485, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87082117, Length=316, Percent_Identity=42.7215189873418, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1789233, Length=323, Percent_Identity=39.3188854489164, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI87082152, Length=301, Percent_Identity=41.8604651162791, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787583, Length=290, Percent_Identity=40.6896551724138, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1788905, Length=310, Percent_Identity=37.741935483871, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=38.3435582822086, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI87081872, Length=296, Percent_Identity=37.8378378378378, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1789828, Length=258, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081858, Length=281, Percent_Identity=29.1814946619217, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 35357; Mature: 35226

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPVALAEIIVGDHPSIVALRDLVLRVARSHASTVLLYGETGTGKGLVARALHEHSSRA
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
DKGFTEINCAAIPGNLLESELFGHERGAFTGAVARKVGLLETAHGGSVFLDEVRELDPMM
CCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHCHHH
QAKILTLLDSRRFRRIGAVREVCTDVRFIAATNKILLGEVNAGKFRDDLYYRLQVVSINL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCCCHHHCCEEEEEEEEECC
PPLRERGDDIFVLAERFLARFNRLHASHFERLDAPVEAAFRRYAWPGNVRELGNLLERIC
CCHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHH
ILESGTRVGLEHLPARVVRGPAPGVDVAEGNYAARTATFQRGMIESALAATGGNLGAAAS
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TLGLSRHALRHQMSKLDMVQGVGRS
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TAPVALAEIIVGDHPSIVALRDLVLRVARSHASTVLLYGETGTGKGLVARALHEHSSRA
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
DKGFTEINCAAIPGNLLESELFGHERGAFTGAVARKVGLLETAHGGSVFLDEVRELDPMM
CCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHCHHH
QAKILTLLDSRRFRRIGAVREVCTDVRFIAATNKILLGEVNAGKFRDDLYYRLQVVSINL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCCCHHHCCEEEEEEEEECC
PPLRERGDDIFVLAERFLARFNRLHASHFERLDAPVEAAFRRYAWPGNVRELGNLLERIC
CCHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHH
ILESGTRVGLEHLPARVVRGPAPGVDVAEGNYAARTATFQRGMIESALAATGGNLGAAAS
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TLGLSRHALRHQMSKLDMVQGVGRS
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]