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The map label for this gene is gcvH [H]

Identifier: 121610876

GI number: 121610876

Start: 4344238

End: 4344612

Strand: Direct

Name: gcvH [H]

Synonym: Veis_3958

Alternate gene names: 121610876

Gene position: 4344238-4344612 (Clockwise)

Preceding gene: 121610875

Following gene: 121610877

Centisome position: 78.04

GC content: 66.13

Gene sequence:

>375_bases
ATGACCGTCAAATTTTCCAAAGACCATGAATGGATCGATGCCGCCGACGCCACTGCCGCCGTGGTCGGCATCACCGTGCA
CGCCCAGGATGCGCTCGGCGACGTGGTGTTCGTCGACCTGCCCGAAGTCGGCAGGCATTTTGCGCAGGGCGAAGTCGCCG
GCGTGGTCGAATCCGTGAAGGCGGCAGCCGACGTGTACATGCCGGTCTCGGGCGAGATCACCGCCGTCAACGAGAGCCTG
CGCGCCGACCCGTCGCTGGCCAACTCCGACCCGCTCGGCGCCGGCTGGTTCTTCAAGATCAGGCCCAGCGATGCCGGCCA
GCTCGATGCGCTGCTCGACGCAGCCAGCTACGCCGACTACGCCAAAACCGCCTGA

Upstream 100 bases:

>100_bases
GGCAAGGCCGTGCCGATGCAGGTGTGTGCCATGCCCTTCGTGCCGGCGAACTACTGCCGCTGATGGTCGATTTTTTTCTT
TTCCTCCCCGAAAGCCTCCG

Downstream 100 bases:

>100_bases
GCCTGTGACCCCTTCTTCGAGACCGCCCCATGCCGATGCCATCTGCTGCGCAGCATCCTGTACCGCTCGGTGCACTGGAA
AACGCCACCGAATTCCTGCC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 123

Protein sequence:

>124_residues
MTVKFSKDHEWIDAADATAAVVGITVHAQDALGDVVFVDLPEVGRHFAQGEVAGVVESVKAAADVYMPVSGEITAVNESL
RADPSLANSDPLGAGWFFKIRPSDAGQLDALLDAASYADYAKTA

Sequences:

>Translated_124_residues
MTVKFSKDHEWIDAADATAAVVGITVHAQDALGDVVFVDLPEVGRHFAQGEVAGVVESVKAAADVYMPVSGEITAVNESL
RADPSLANSDPLGAGWFFKIRPSDAGQLDALLDAASYADYAKTA
>Mature_123_residues
TVKFSKDHEWIDAADATAAVVGITVHAQDALGDVVFVDLPEVGRHFAQGEVAGVVESVKAAADVYMPVSGEITAVNESLR
ADPSLANSDPLGAGWFFKIRPSDAGQLDALLDAASYADYAKTA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=41.6666666666667, Blast_Score=108, Evalue=1e-24,
Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=41.6666666666667, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789271, Length=115, Percent_Identity=54.7826086956522, Blast_Score=132, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=42.2413793103448, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=40.6779661016949, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=38.8429752066116, Blast_Score=109, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17865652, Length=117, Percent_Identity=40.1709401709402, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 12963; Mature: 12832

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKFSKDHEWIDAADATAAVVGITVHAQDALGDVVFVDLPEVGRHFAQGEVAGVVESVK
CCEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECHHHHHHHHCCHHHHHHHHHH
AAADVYMPVSGEITAVNESLRADPSLANSDPLGAGWFFKIRPSDAGQLDALLDAASYADY
HHHHEECCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
AKTA
HCCC
>Mature Secondary Structure 
TVKFSKDHEWIDAADATAAVVGITVHAQDALGDVVFVDLPEVGRHFAQGEVAGVVESVK
CEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECHHHHHHHHCCHHHHHHHHHH
AAADVYMPVSGEITAVNESLRADPSLANSDPLGAGWFFKIRPSDAGQLDALLDAASYADY
HHHHEECCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
AKTA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]