Click here to switch to the map view.

The map label for this gene is pvuIIM [H]

Identifier: 121610449

GI number: 121610449

Start: 3890138

End: 3891133

Strand: Direct

Name: pvuIIM [H]

Synonym: Veis_3519

Alternate gene names: 121610449

Gene position: 3890138-3891133 (Clockwise)

Preceding gene: 121610446

Following gene: 121610450

Centisome position: 69.88

GC content: 49.6

Gene sequence:

>996_bases
ATGACACCTGATCTGCCCGTTTTCAACCAGCAGCCGCTCTACCACACTGACTTGGGGGCGGCTTACGTCGCTGATTCGCT
CGACATGCTCCGGCAGTTGCCTGACAACAGCATCAACTTGGTCATGACCTCTCCCCCTTTTGCGCTTCAGCGTCAGAAGG
ACTACGGAAACAAGGATCAGCATGAATACATTGACTGGTTAACCGAATTCGCCACGCTCGTTTACCAAAAGCTCACACCT
GATGGTTCTTTTGTGCTGGACTTGGGGGGAGCGTATCAGAAGGGCTTACCCGTTCGCAGCCTCTACAACTATCGTGTCCT
GATCAGGTTCTGTGACGAGATCGGCTTCCACTTGGCCGAAGAATTCTTCTGGTTCAATCCCTCCAAGTTGCCCTCCCCGA
TCGAGTGGGTCAACAAGCGGAAAATTCGTGCCAAAGACTCCGTTAACACCGTTTGGTGGTTGTCAAAAACTGAGTGGCCG
AAGGCAGATGTCTCCAAGGTCCTGGCCGAATATTCGGAGCGGATGAAAAAACTCATCAAAGATCCAGAAGCCTACTACTC
ACCGGCGAAACGACCATCAGGCCATGATATTGGTTCTTCTTTTGGCAAGGACAACGGAGGATCCATTCCTTCCAACTTGC
TTCAAATTTCCAACTCTGAAGCAAATTCCCAATATCTGAAGCTGTGCAAAGAAGTCGGGATTCAAGCTCACCCTGCGCGA
TTTCCCGCCAAATTACCTGAATTTTTCATCAGGTATCTGACAGAACCCAACGACCTGGTTGTGGACATCTTCGGCGGCTC
AAACACCACGGGTCATGTTTGCGAATCGGAAGGCAGGCGCTGGCTGGCATTTGAACAACTTCAAGAGTATCTGTCTGCTT
CCGTGTTCAGGTTCATGCAGGATCAACCAATCGAGAGCATCAAGACCACCTTTGCAGAATTAATGGAAAACAAGATCAAA
CACATCAAATCAAATGGGCAAGGCAAGTTGTTCTAA

Upstream 100 bases:

>100_bases
GCACATGGATGAAGTTCTCAATGATCTCCGCTTGACAACTTTGAATCTACAGACTATAAGTTTCATCCTTTGAGGATTGC
ACAAATCTGACCACATCGCC

Downstream 100 bases:

>100_bases
CCTGGCCATCCCGGAATATGAACTGGCAAAAGCCGCAGATCGTCCAGCGTGAACGTCCGCTCGCGCGACACATGGCCGGT
GACCCTCAAGACCGGCTCGG

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.PvuII; N-4 cytosine-specific methyltransferase PvuII [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTPDLPVFNQQPLYHTDLGAAYVADSLDMLRQLPDNSINLVMTSPPFALQRQKDYGNKDQHEYIDWLTEFATLVYQKLTP
DGSFVLDLGGAYQKGLPVRSLYNYRVLIRFCDEIGFHLAEEFFWFNPSKLPSPIEWVNKRKIRAKDSVNTVWWLSKTEWP
KADVSKVLAEYSERMKKLIKDPEAYYSPAKRPSGHDIGSSFGKDNGGSIPSNLLQISNSEANSQYLKLCKEVGIQAHPAR
FPAKLPEFFIRYLTEPNDLVVDIFGGSNTTGHVCESEGRRWLAFEQLQEYLSASVFRFMQDQPIESIKTTFAELMENKIK
HIKSNGQGKLF

Sequences:

>Translated_331_residues
MTPDLPVFNQQPLYHTDLGAAYVADSLDMLRQLPDNSINLVMTSPPFALQRQKDYGNKDQHEYIDWLTEFATLVYQKLTP
DGSFVLDLGGAYQKGLPVRSLYNYRVLIRFCDEIGFHLAEEFFWFNPSKLPSPIEWVNKRKIRAKDSVNTVWWLSKTEWP
KADVSKVLAEYSERMKKLIKDPEAYYSPAKRPSGHDIGSSFGKDNGGSIPSNLLQISNSEANSQYLKLCKEVGIQAHPAR
FPAKLPEFFIRYLTEPNDLVVDIFGGSNTTGHVCESEGRRWLAFEQLQEYLSASVFRFMQDQPIESIKTTFAELMENKIK
HIKSNGQGKLF
>Mature_330_residues
TPDLPVFNQQPLYHTDLGAAYVADSLDMLRQLPDNSINLVMTSPPFALQRQKDYGNKDQHEYIDWLTEFATLVYQKLTPD
GSFVLDLGGAYQKGLPVRSLYNYRVLIRFCDEIGFHLAEEFFWFNPSKLPSPIEWVNKRKIRAKDSVNTVWWLSKTEWPK
ADVSKVLAEYSERMKKLIKDPEAYYSPAKRPSGHDIGSSFGKDNGGSIPSNLLQISNSEANSQYLKLCKEVGIQAHPARF
PAKLPEFFIRYLTEPNDLVVDIFGGSNTTGHVCESEGRRWLAFEQLQEYLSASVFRFMQDQPIESIKTTFAELMENKIKH
IKSNGQGKLF

Specific function: This methylase recognizes the double-stranded sequence CAGCTG, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PvuII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 37891; Mature: 37760

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDLPVFNQQPLYHTDLGAAYVADSLDMLRQLPDNSINLVMTSPPFALQRQKDYGNKDQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCHH
HEYIDWLTEFATLVYQKLTPDGSFVLDLGGAYQKGLPVRSLYNYRVLIRFCDEIGFHLAE
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EFFWFNPSKLPSPIEWVNKRKIRAKDSVNTVWWLSKTEWPKADVSKVLAEYSERMKKLIK
HHHCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
DPEAYYSPAKRPSGHDIGSSFGKDNGGSIPSNLLQISNSEANSQYLKLCKEVGIQAHPAR
CCHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
FPAKLPEFFIRYLTEPNDLVVDIFGGSNTTGHVCESEGRRWLAFEQLQEYLSASVFRFMQ
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHH
DQPIESIKTTFAELMENKIKHIKSNGQGKLF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TPDLPVFNQQPLYHTDLGAAYVADSLDMLRQLPDNSINLVMTSPPFALQRQKDYGNKDQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCHH
HEYIDWLTEFATLVYQKLTPDGSFVLDLGGAYQKGLPVRSLYNYRVLIRFCDEIGFHLAE
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EFFWFNPSKLPSPIEWVNKRKIRAKDSVNTVWWLSKTEWPKADVSKVLAEYSERMKKLIK
HHHCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
DPEAYYSPAKRPSGHDIGSSFGKDNGGSIPSNLLQISNSEANSQYLKLCKEVGIQAHPAR
CCHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
FPAKLPEFFIRYLTEPNDLVVDIFGGSNTTGHVCESEGRRWLAFEQLQEYLSASVFRFMQ
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHH
DQPIESIKTTFAELMENKIKHIKSNGQGKLF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2662138; 7607491; 9207015 [H]