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The map label for this gene is afr [H]

Identifier: 121610365

GI number: 121610365

Start: 3804743

End: 3805834

Strand: Direct

Name: afr [H]

Synonym: Veis_3433

Alternate gene names: 121610365

Gene position: 3804743-3805834 (Clockwise)

Preceding gene: 121610364

Following gene: 121610366

Centisome position: 68.35

GC content: 59.43

Gene sequence:

>1092_bases
ATGAAGATACTACCGCAGCCTGACGTGCCCATCCGCTGCAGAGAGTTCCGCATCGGCGCTATCGGTGCCGGGTTCATCAT
GAGTGAAGTGCAACTGGAGGCATACCGCGAAGCCAACTTCAGCGTGCTTGCCATTGCATCGCGCACGCGCTCCAAGGCCG
AGGCGGCCGCAAAGCGTTACGGAATTCTCACCGTTCACGATACCCCCGAAGACCTCATCCGCGACCCCCGGGTCGAGATT
GTCGATATCGCGTTCCCCCCGGACATGCAACCCGGCCTGATACGCTTGGCGCTCCAGCAGCCACATATCAAGGGCATCTT
GGCGCAAAAGCCGCTGGCGCTCGATTTTGTGACGGCACGCGAACCGGCCAGGGAGTGCAGGACGTCGGGAAAGCGGGTGT
CGGTCAACCAGAATATGCGCTTCGATCAATCGATGCGGGTACTCAAACAGATTCTCGACAACGGTGAGTTGGGTCTCCCC
GTCCTGGCTACGATAGAAATGCGGGCGATTCCGCATTGGCAGGGCTTCCTGAAACAGTATGGACGGCTGACCTTGCTGAA
TATGAGCATCCATCACCTGGATGTGTTGCGCTTTCTCTTCGGTGAGCCCATCGACATCTTCAGCGCCGCGCGCCCGGACC
CCCGCACCGAGTTTGCGCACACCGACGGCATCGTTTGCTCCACGCTCCGCTTTCAGAACGGACTGATGGCCGTCAGTCTG
GAGGATGTGTGGAGCGGGCCGCGCGAGGCAAACTTTGCATCGGATACCTATATCAAATGGCGTGTAGAAGGCACTGAGGG
TCTGGCGCAGGGCAGTATCGGCTGGCCTGATTACCCCGCAGGCAGTCCCAGCACACTGCGATACTGTTCGCGCAGAACGA
CCGACGGTCGCTGGATCGAGCCGACCTGGGGCACATGCTGGTTTCCCCAGGCATTTGCCGGTGTGATGGAGCAACTGCAA
TGGGCCTTGAAAAGCAATCAGCCGCCCATGTTGGGCGTAGATGACAATCTCAGGACGATGGCTCTGGTCGAGGCCGGCTA
TCGTTCGATGGCAGAGCGGCGCACCGTCTTGCTTGCTGAATTTGAGGTATGA

Upstream 100 bases:

>100_bases
ATGGCCAGCGCGGACCGACAGATGATCGGTGACGCGACACTAGGGTGCACCACGCTTGCCGGTGGACCACCGCTGCATCG
ACAAAATATCGGAGACAAAC

Downstream 100 bases:

>100_bases
GTTGGATAGCGGTTTCGTGTAACCCGGCAGGAGGAATTTACCATGATTCAAACAGGAATCTTCACCGGCTATTTTCCTTA
TTCACTGGAGGAATCCGCCC

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKILPQPDVPIRCREFRIGAIGAGFIMSEVQLEAYREANFSVLAIASRTRSKAEAAAKRYGILTVHDTPEDLIRDPRVEI
VDIAFPPDMQPGLIRLALQQPHIKGILAQKPLALDFVTAREPARECRTSGKRVSVNQNMRFDQSMRVLKQILDNGELGLP
VLATIEMRAIPHWQGFLKQYGRLTLLNMSIHHLDVLRFLFGEPIDIFSAARPDPRTEFAHTDGIVCSTLRFQNGLMAVSL
EDVWSGPREANFASDTYIKWRVEGTEGLAQGSIGWPDYPAGSPSTLRYCSRRTTDGRWIEPTWGTCWFPQAFAGVMEQLQ
WALKSNQPPMLGVDDNLRTMALVEAGYRSMAERRTVLLAEFEV

Sequences:

>Translated_363_residues
MKILPQPDVPIRCREFRIGAIGAGFIMSEVQLEAYREANFSVLAIASRTRSKAEAAAKRYGILTVHDTPEDLIRDPRVEI
VDIAFPPDMQPGLIRLALQQPHIKGILAQKPLALDFVTAREPARECRTSGKRVSVNQNMRFDQSMRVLKQILDNGELGLP
VLATIEMRAIPHWQGFLKQYGRLTLLNMSIHHLDVLRFLFGEPIDIFSAARPDPRTEFAHTDGIVCSTLRFQNGLMAVSL
EDVWSGPREANFASDTYIKWRVEGTEGLAQGSIGWPDYPAGSPSTLRYCSRRTTDGRWIEPTWGTCWFPQAFAGVMEQLQ
WALKSNQPPMLGVDDNLRTMALVEAGYRSMAERRTVLLAEFEV
>Mature_363_residues
MKILPQPDVPIRCREFRIGAIGAGFIMSEVQLEAYREANFSVLAIASRTRSKAEAAAKRYGILTVHDTPEDLIRDPRVEI
VDIAFPPDMQPGLIRLALQQPHIKGILAQKPLALDFVTAREPARECRTSGKRVSVNQNMRFDQSMRVLKQILDNGELGLP
VLATIEMRAIPHWQGFLKQYGRLTLLNMSIHHLDVLRFLFGEPIDIFSAARPDPRTEFAHTDGIVCSTLRFQNGLMAVSL
EDVWSGPREANFASDTYIKWRVEGTEGLAQGSIGWPDYPAGSPSTLRYCSRRTTDGRWIEPTWGTCWFPQAFAGVMEQLQ
WALKSNQPPMLGVDDNLRTMALVEAGYRSMAERRTVLLAEFEV

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1787574, Length=194, Percent_Identity=25.7731958762887, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 40883; Mature: 40883

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILPQPDVPIRCREFRIGAIGAGFIMSEVQLEAYREANFSVLAIASRTRSKAEAAAKRY
CCCCCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHC
GILTVHDTPEDLIRDPRVEIVDIAFPPDMQPGLIRLALQQPHIKGILAQKPLALDFVTAR
CEEEEECCHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHCCCCEEEEEHHC
EPARECRTSGKRVSVNQNMRFDQSMRVLKQILDNGELGLPVLATIEMRAIPHWQGFLKQY
CHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHC
GRLTLLNMSIHHLDVLRFLFGEPIDIFSAARPDPRTEFAHTDGIVCSTLRFQNGLMAVSL
CCEEEEECHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
EDVWSGPREANFASDTYIKWRVEGTEGLAQGSIGWPDYPAGSPSTLRYCSRRTTDGRWIE
HHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEC
PTWGTCWFPQAFAGVMEQLQWALKSNQPPMLGVDDNLRTMALVEAGYRSMAERRTVLLAE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEEEE
FEV
ECC
>Mature Secondary Structure
MKILPQPDVPIRCREFRIGAIGAGFIMSEVQLEAYREANFSVLAIASRTRSKAEAAAKRY
CCCCCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHC
GILTVHDTPEDLIRDPRVEIVDIAFPPDMQPGLIRLALQQPHIKGILAQKPLALDFVTAR
CEEEEECCHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHCCCCEEEEEHHC
EPARECRTSGKRVSVNQNMRFDQSMRVLKQILDNGELGLPVLATIEMRAIPHWQGFLKQY
CHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHC
GRLTLLNMSIHHLDVLRFLFGEPIDIFSAARPDPRTEFAHTDGIVCSTLRFQNGLMAVSL
CCEEEEECHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
EDVWSGPREANFASDTYIKWRVEGTEGLAQGSIGWPDYPAGSPSTLRYCSRRTTDGRWIE
HHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEC
PTWGTCWFPQAFAGVMEQLQWALKSNQPPMLGVDDNLRTMALVEAGYRSMAERRTVLLAE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEEEE
FEV
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]