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The map label for this gene is alkK [H]

Identifier: 121609869

GI number: 121609869

Start: 3269763

End: 3271427

Strand: Direct

Name: alkK [H]

Synonym: Veis_2922

Alternate gene names: 121609869

Gene position: 3269763-3271427 (Clockwise)

Preceding gene: 121609868

Following gene: 121609870

Centisome position: 58.74

GC content: 68.53

Gene sequence:

>1665_bases
ATGAACACCGAACCCCAAGCCCTGGACGCAGGCCCACCGTCCACGATGATGGACGACGATTTGTCGCTCAACCTGCTGCT
GGAACGGGCCGGCCAACTGTTCGGCAGCAGCACCCTGGTCTCGCGCCTGCCGGACAAATCGCTGACGCGGCACAGCTACG
CCCAGTGGTACCGGCGCACCCGGGCGCTGGCGGCCGCATTGCAGGGCCTGGGCGTGCGCAAGGGCGAGCGCGTGGCCACG
CTGTGCTGGAACCACCATGCGCATCTGGAGTGCTACTTCGCCGTTCCCGCAGCGGGCGCGGTGCTGCACACGCTCAATTT
GCGCCTGTCGCCCGCCGAGATCGGCTGGATCGCCGCCGATGGCGGCGCCCGCGTGCTGATCGTCGACGACATTTTGCTGC
CGCTGTACCGGCAGTTTGCCCATCTGTGCCGCTTCGAGCAGGTGGTGGTGTTTGCGTTCTCGGGCGCGCCGGTGGCTCCC
GATGGCCTGGACTACGAGCAACTGCTGCGCGCCGCCGATGGCGCCGCCTTCCGGTATGTGCCGCACTGCGAGCGCGACCC
GGTCGCCATGTGCTACACCTCGGGCACGACGGGCCGGCCCAAAGGCGTGGTCTACTCGCACCGCTCGGCCATGCTCCACA
CCCTGACGGCCTGCCTGCCGGACTACTGGAACCTGCGCGGGGAGGATGTCGTGCTGCCGGTCACGCCGATGTTCCATGCC
AATAGCTGGGGCATTCCATACGGCGCAGTCATGCTCGGCTGCAAACTGGTGTTCCCCGGGCCGCACCTGCATGCGCCAGA
CCTGATGGATTTGATGACCGAAGAGCAGCCGACCTTTGCGCTCGGCGTGCCAACCATCTGGATGGGCATCGCCCAGGGCT
ACAGCGCCGCGCAAGACCCCGCCCATCCCGACCATGGCCGCTGGCGGCTGCCCCCGGGCATGCGCTCGATCGTCGGCGGA
GCGGCGGTGCCCGAGTCCCTGATCCGCACGCTGGCCACGCAGGGACTGGGGATCGTGCAGGGCTGGGGCATGACCGAGAC
CTCGCCGCTGGCCACCGTGTCCTACCTCAAGCCCGAGTTGCGCGCAGCCTCGGCAGACGAGCGCTACCGGCGCGCGGCCA
TGGCCGGCGTGCCCTTGCCGCTGGTGCAATTGCGGCTGCAGCGCGAGGACGGCAGCCTGGCGCCCTGGGACGGGCAAAGC
CCCGGCGAGATCCAGGTGCGCGGCCCCTACATCACTGGCTCGTATGCGGGCATTGGCTCGCCGCCGGACAACTTCACCCC
CGATGGCTGGCTGCGCACCGGCGATGTCGGCCACTGCGATGCCCATGGCTACTTGTGCCTGACCGACCGGACCAAGGACC
TGATCAAGTCGGGCGGCGAATGGATCAGCTCGATGCAACTGGAGAACGCGCTGATGGGACACCCGGCAGTGGCCGAGGCC
GCCGTCGTGGCCATTCCCGACCAGCGCTGGAGCGAGCGGCCGCTGGCCTGCGTGGTGCTCCAGCCCGCGCGCCAGGCCAC
GCCAGAGCAGTTGAACCGGCATTTGGGCGAGCAGGGCTTCAGCAAGTGGCAGTGGCCCGAGCGCTACGAATTCATGGACG
CCATTCCCAAGACCGCCACCGGCAAGTTCTGGAAACTGCGGCTGCGCGAGCTATTTCCGGCCTGA

Upstream 100 bases:

>100_bases
GACTGCACTACTTCGAGCCCGAAGGCGACGAGCCGCCCAATGCCGGCGCGCTCGAGCAGATGAACCAATTGCTACCCTGG
CTGCAGGAGCGTTGCCCGCC

Downstream 100 bases:

>100_bases
GCGGGTGCCCCGGCCGCCAGATCGCCAGGTCGCCAAGTACGCTATCTGGCCCCCTGGCGCATGGCGCAACCGCCAGGGCC
GGAACCCGCCATGCCCGACA

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MNTEPQALDAGPPSTMMDDDLSLNLLLERAGQLFGSSTLVSRLPDKSLTRHSYAQWYRRTRALAAALQGLGVRKGERVAT
LCWNHHAHLECYFAVPAAGAVLHTLNLRLSPAEIGWIAADGGARVLIVDDILLPLYRQFAHLCRFEQVVVFAFSGAPVAP
DGLDYEQLLRAADGAAFRYVPHCERDPVAMCYTSGTTGRPKGVVYSHRSAMLHTLTACLPDYWNLRGEDVVLPVTPMFHA
NSWGIPYGAVMLGCKLVFPGPHLHAPDLMDLMTEEQPTFALGVPTIWMGIAQGYSAAQDPAHPDHGRWRLPPGMRSIVGG
AAVPESLIRTLATQGLGIVQGWGMTETSPLATVSYLKPELRAASADERYRRAAMAGVPLPLVQLRLQREDGSLAPWDGQS
PGEIQVRGPYITGSYAGIGSPPDNFTPDGWLRTGDVGHCDAHGYLCLTDRTKDLIKSGGEWISSMQLENALMGHPAVAEA
AVVAIPDQRWSERPLACVVLQPARQATPEQLNRHLGEQGFSKWQWPERYEFMDAIPKTATGKFWKLRLRELFPA

Sequences:

>Translated_554_residues
MNTEPQALDAGPPSTMMDDDLSLNLLLERAGQLFGSSTLVSRLPDKSLTRHSYAQWYRRTRALAAALQGLGVRKGERVAT
LCWNHHAHLECYFAVPAAGAVLHTLNLRLSPAEIGWIAADGGARVLIVDDILLPLYRQFAHLCRFEQVVVFAFSGAPVAP
DGLDYEQLLRAADGAAFRYVPHCERDPVAMCYTSGTTGRPKGVVYSHRSAMLHTLTACLPDYWNLRGEDVVLPVTPMFHA
NSWGIPYGAVMLGCKLVFPGPHLHAPDLMDLMTEEQPTFALGVPTIWMGIAQGYSAAQDPAHPDHGRWRLPPGMRSIVGG
AAVPESLIRTLATQGLGIVQGWGMTETSPLATVSYLKPELRAASADERYRRAAMAGVPLPLVQLRLQREDGSLAPWDGQS
PGEIQVRGPYITGSYAGIGSPPDNFTPDGWLRTGDVGHCDAHGYLCLTDRTKDLIKSGGEWISSMQLENALMGHPAVAEA
AVVAIPDQRWSERPLACVVLQPARQATPEQLNRHLGEQGFSKWQWPERYEFMDAIPKTATGKFWKLRLRELFPA
>Mature_554_residues
MNTEPQALDAGPPSTMMDDDLSLNLLLERAGQLFGSSTLVSRLPDKSLTRHSYAQWYRRTRALAAALQGLGVRKGERVAT
LCWNHHAHLECYFAVPAAGAVLHTLNLRLSPAEIGWIAADGGARVLIVDDILLPLYRQFAHLCRFEQVVVFAFSGAPVAP
DGLDYEQLLRAADGAAFRYVPHCERDPVAMCYTSGTTGRPKGVVYSHRSAMLHTLTACLPDYWNLRGEDVVLPVTPMFHA
NSWGIPYGAVMLGCKLVFPGPHLHAPDLMDLMTEEQPTFALGVPTIWMGIAQGYSAAQDPAHPDHGRWRLPPGMRSIVGG
AAVPESLIRTLATQGLGIVQGWGMTETSPLATVSYLKPELRAASADERYRRAAMAGVPLPLVQLRLQREDGSLAPWDGQS
PGEIQVRGPYITGSYAGIGSPPDNFTPDGWLRTGDVGHCDAHGYLCLTDRTKDLIKSGGEWISSMQLENALMGHPAVAEA
AVVAIPDQRWSERPLACVVLQPARQATPEQLNRHLGEQGFSKWQWPERYEFMDAIPKTATGKFWKLRLRELFPA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=561, Percent_Identity=24.2424242424242, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI187761345, Length=550, Percent_Identity=26, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI187761343, Length=550, Percent_Identity=26, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI157311624, Length=496, Percent_Identity=24.7983870967742, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI157311622, Length=496, Percent_Identity=24.7983870967742, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI58082049, Length=520, Percent_Identity=23.8461538461538, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI38505220, Length=520, Percent_Identity=24.0384615384615, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=24.0234375, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI122937307, Length=493, Percent_Identity=23.9350912778905, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI115511026, Length=401, Percent_Identity=26.1845386533666, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI13375727, Length=414, Percent_Identity=24.6376811594203, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788107, Length=531, Percent_Identity=25.6120527306968, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI221142682, Length=526, Percent_Identity=25.2851711026616, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI145693145, Length=515, Percent_Identity=26.2135922330097, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1786810, Length=152, Percent_Identity=37.5, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790505, Length=587, Percent_Identity=24.3611584327087, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789201, Length=372, Percent_Identity=24.1935483870968, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788595, Length=340, Percent_Identity=24.4117647058824, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560308, Length=567, Percent_Identity=25.0440917107584, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=580, Percent_Identity=24.4827586206897, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=580, Percent_Identity=24.4827586206897, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=580, Percent_Identity=24.4827586206897, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=511, Percent_Identity=26.8101761252446, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=511, Percent_Identity=25.440313111546, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=501, Percent_Identity=24.5508982035928, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=514, Percent_Identity=25.4863813229572, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=519, Percent_Identity=26.3969171483622, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=512, Percent_Identity=25.390625, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71996755, Length=514, Percent_Identity=23.7354085603113, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=552, Percent_Identity=22.6449275362319, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=383, Percent_Identity=25.065274151436, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556552, Length=190, Percent_Identity=27.8947368421053, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=370, Percent_Identity=28.1081081081081, Blast_Score=123, Evalue=9e-29,
Organism=Drosophila melanogaster, GI18859661, Length=524, Percent_Identity=29.0076335877863, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21355181, Length=515, Percent_Identity=24.0776699029126, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24581924, Length=541, Percent_Identity=26.0628465804067, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356947, Length=404, Percent_Identity=23.7623762376238, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355225, Length=530, Percent_Identity=23.5849056603774, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648260, Length=368, Percent_Identity=24.4565217391304, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653035, Length=380, Percent_Identity=22.3684210526316, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60732; Mature: 60732

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEPQALDAGPPSTMMDDDLSLNLLLERAGQLFGSSTLVSRLPDKSLTRHSYAQWYRRT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
RALAAALQGLGVRKGERVATLCWNHHAHLECYFAVPAAGAVLHTLNLRLSPAEIGWIAAD
HHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEECEECCCCEEEEEEC
GGARVLIVDDILLPLYRQFAHLCRFEQVVVFAFSGAPVAPDGLDYEQLLRAADGAAFRYV
CCCEEEEEHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEC
PHCERDPVAMCYTSGTTGRPKGVVYSHRSAMLHTLTACLPDYWNLRGEDVVLPVTPMFHA
CCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEC
NSWGIPYGAVMLGCKLVFPGPHLHAPDLMDLMTEEQPTFALGVPTIWMGIAQGYSAAQDP
CCCCCCHHHHHHCCEEECCCCCCCCCHHHHHHCCCCCCEEECCHHHHHHHHCCCCCCCCC
AHPDHGRWRLPPGMRSIVGGAAVPESLIRTLATQGLGIVQGWGMTETSPLATVSYLKPEL
CCCCCCCEECCCCHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHCHHH
RAASADERYRRAAMAGVPLPLVQLRLQREDGSLAPWDGQSPGEIQVRGPYITGSYAGIGS
HCCCHHHHHHHHHHCCCCHHHHHHEEECCCCCCCCCCCCCCCEEEEECCEEECCCCCCCC
PPDNFTPDGWLRTGDVGHCDAHGYLCLTDRTKDLIKSGGEWISSMQLENALMGHPAVAEA
CCCCCCCCCCEECCCCCCCCCCCEEEEECCHHHHHHCCHHHHHHHHHHHHHCCCCCHHCE
AVVAIPDQRWSERPLACVVLQPARQATPEQLNRHLGEQGFSKWQWPERYEFMDAIPKTAT
EEEEECCCCCCCCCEEEEEECCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
GKFWKLRLRELFPA
CHHHHHHHHHHCCC
>Mature Secondary Structure
MNTEPQALDAGPPSTMMDDDLSLNLLLERAGQLFGSSTLVSRLPDKSLTRHSYAQWYRRT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
RALAAALQGLGVRKGERVATLCWNHHAHLECYFAVPAAGAVLHTLNLRLSPAEIGWIAAD
HHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEECEECCCCEEEEEEC
GGARVLIVDDILLPLYRQFAHLCRFEQVVVFAFSGAPVAPDGLDYEQLLRAADGAAFRYV
CCCEEEEEHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEC
PHCERDPVAMCYTSGTTGRPKGVVYSHRSAMLHTLTACLPDYWNLRGEDVVLPVTPMFHA
CCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEC
NSWGIPYGAVMLGCKLVFPGPHLHAPDLMDLMTEEQPTFALGVPTIWMGIAQGYSAAQDP
CCCCCCHHHHHHCCEEECCCCCCCCCHHHHHHCCCCCCEEECCHHHHHHHHCCCCCCCCC
AHPDHGRWRLPPGMRSIVGGAAVPESLIRTLATQGLGIVQGWGMTETSPLATVSYLKPEL
CCCCCCCEECCCCHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHCHHH
RAASADERYRRAAMAGVPLPLVQLRLQREDGSLAPWDGQSPGEIQVRGPYITGSYAGIGS
HCCCHHHHHHHHHHCCCCHHHHHHEEECCCCCCCCCCCCCCCEEEEECCEEECCCCCCCC
PPDNFTPDGWLRTGDVGHCDAHGYLCLTDRTKDLIKSGGEWISSMQLENALMGHPAVAEA
CCCCCCCCCCEECCCCCCCCCCCEEEEECCHHHHHHCCHHHHHHHHHHHHHCCCCCHHCE
AVVAIPDQRWSERPLACVVLQPARQATPEQLNRHLGEQGFSKWQWPERYEFMDAIPKTAT
EEEEECCCCCCCCCEEEEEECCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
GKFWKLRLRELFPA
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]