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The map label for this gene is rbsB [H]

Identifier: 121609635

GI number: 121609635

Start: 3025189

End: 3026175

Strand: Direct

Name: rbsB [H]

Synonym: Veis_2682

Alternate gene names: 121609635

Gene position: 3025189-3026175 (Clockwise)

Preceding gene: 121609620

Following gene: 121609636

Centisome position: 54.34

GC content: 66.16

Gene sequence:

>987_bases
ATGAGAAATCGAATTGCCCCCGCTTTGCGCGCTCTTTTCGGCGCGGCCGTCGTTGCAGCCGGTGTCGCCGTGCTGACCCC
CGTGGCCCATGCGGCGGGTCTGAAAATCGGCTTCAGCCAGGTGACCTTGCAGTCCCCGTTCTACGTGCAGCTCAAGGAGG
GCGCAGAGGCGGCCGCCAAGGCCGGCGGCGACGAACTGATCTTTCTGGATGCCAATGGCGACGTCAACAAGCAGAACAAC
GACATCCAGGACCTGCTGACCCGCAAGGTCGACGTGCTGATCATCAATCCGGTCAATCCCGACGCCGTCAAGCCGGCCAT
TGCCGCGGCGCAAAAGAACGGCGTGCCGGTCATCACCGTCGACCGCAGCATCAACGGTGGCGGCGTGACGGCGCACCTGG
GCCGCGACAACGTGAAGATGGGCAAGGCCGTGGGCGATGCCGTGCTGGCCGAGCTCAAGAAGAAGGGCACGACCCGGGGC
AAGGTCATCGAGATCCAGGGCGACGCCGGTGGCACCGTGATGGCGGCGCGGCGCGACGGTTTCCACGACGCGCTCAAGGG
TTCGGGCTTCAAGATCGTGGCCGGTCCCTATGCCGAGTACACGCGCGCCAACGCAGTGACCGCCATGCAGGACCTGCTGC
AGGCCCACCCCGATGTGAAGGTGGTCTACGCGCACAACGACGACATGGCCATGGGCGCACTCAAGGTGCTCGCCGAGGCC
GGCAAGAAAGACGTGTTCGTGGCCGGCGTGGACGGCCTCTCCGAAGCCCTGGCCAGCATGGCCGGCGGCGACCAGTACGT
GGCCACCGCGCTGAACGACCCGCGCTACCTGGGCGACATGACGGTGCAGACCGCGCGCGCGATCAAGGCCGGCAAGAAGG
TGCCCGCCTTCGTGGACGCCGGCACCAAGGCCGTGACCAAAGCCAACGTGGGCGAGTTCAAGCGCACCGGTCTGTTTGCC
GAGTACCGGCCTGCGGTCTTCAAGTAA

Upstream 100 bases:

>100_bases
ATTTCCTCACTCGAAGTTCCTTTGGGTGTTTCATAATTTTCTGCAGATGAGTGCCAGGTGTTCATCTGAACAGAATCAAA
CGCAGTCCATTGGAGACATC

Downstream 100 bases:

>100_bases
AGCCGGCCTGTTTTCTCCCTGTTTTTCTCCCTCCTTGCCGGGCAGGCAGGGGGCCTGCCTTCATCCATCGTCCGCACATT
CCATGAACTCCAGCCATCCC

Product: periplasmic-binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRNRIAPALRALFGAAVVAAGVAVLTPVAHAAGLKIGFSQVTLQSPFYVQLKEGAEAAAKAGGDELIFLDANGDVNKQNN
DIQDLLTRKVDVLIINPVNPDAVKPAIAAAQKNGVPVITVDRSINGGGVTAHLGRDNVKMGKAVGDAVLAELKKKGTTRG
KVIEIQGDAGGTVMAARRDGFHDALKGSGFKIVAGPYAEYTRANAVTAMQDLLQAHPDVKVVYAHNDDMAMGALKVLAEA
GKKDVFVAGVDGLSEALASMAGGDQYVATALNDPRYLGDMTVQTARAIKAGKKVPAFVDAGTKAVTKANVGEFKRTGLFA
EYRPAVFK

Sequences:

>Translated_328_residues
MRNRIAPALRALFGAAVVAAGVAVLTPVAHAAGLKIGFSQVTLQSPFYVQLKEGAEAAAKAGGDELIFLDANGDVNKQNN
DIQDLLTRKVDVLIINPVNPDAVKPAIAAAQKNGVPVITVDRSINGGGVTAHLGRDNVKMGKAVGDAVLAELKKKGTTRG
KVIEIQGDAGGTVMAARRDGFHDALKGSGFKIVAGPYAEYTRANAVTAMQDLLQAHPDVKVVYAHNDDMAMGALKVLAEA
GKKDVFVAGVDGLSEALASMAGGDQYVATALNDPRYLGDMTVQTARAIKAGKKVPAFVDAGTKAVTKANVGEFKRTGLFA
EYRPAVFK
>Mature_328_residues
MRNRIAPALRALFGAAVVAAGVAVLTPVAHAAGLKIGFSQVTLQSPFYVQLKEGAEAAAKAGGDELIFLDANGDVNKQNN
DIQDLLTRKVDVLIINPVNPDAVKPAIAAAQKNGVPVITVDRSINGGGVTAHLGRDNVKMGKAVGDAVLAELKKKGTTRG
KVIEIQGDAGGTVMAARRDGFHDALKGSGFKIVAGPYAEYTRANAVTAMQDLLQAHPDVKVVYAHNDDMAMGALKVLAEA
GKKDVFVAGVDGLSEALASMAGGDQYVATALNDPRYLGDMTVQTARAIKAGKKVPAFVDAGTKAVTKANVGEFKRTGLFA
EYRPAVFK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=266, Percent_Identity=39.4736842105263, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1790526, Length=266, Percent_Identity=32.7067669172932, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790674, Length=248, Percent_Identity=28.6290322580645, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788473, Length=300, Percent_Identity=29.6666666666667, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788898, Length=211, Percent_Identity=27.9620853080569, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34117; Mature: 34117

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNRIAPALRALFGAAVVAAGVAVLTPVAHAAGLKIGFSQVTLQSPFYVQLKEGAEAAAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEECCCEEEEECCCCHHHHH
AGGDELIFLDANGDVNKQNNDIQDLLTRKVDVLIINPVNPDAVKPAIAAAQKNGVPVITV
CCCCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEE
DRSINGGGVTAHLGRDNVKMGKAVGDAVLAELKKKGTTRGKVIEIQGDAGGTVMAARRDG
ECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCC
FHDALKGSGFKIVAGPYAEYTRANAVTAMQDLLQAHPDVKVVYAHNDDMAMGALKVLAEA
CHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHC
GKKDVFVAGVDGLSEALASMAGGDQYVATALNDPRYLGDMTVQTARAIKAGKKVPAFVDA
CCCCEEEECCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEECC
GTKAVTKANVGEFKRTGLFAEYRPAVFK
CCHHHHHCCCCHHHHHCCCHHCCCCCCC
>Mature Secondary Structure
MRNRIAPALRALFGAAVVAAGVAVLTPVAHAAGLKIGFSQVTLQSPFYVQLKEGAEAAAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEECCCEEEEECCCCHHHHH
AGGDELIFLDANGDVNKQNNDIQDLLTRKVDVLIINPVNPDAVKPAIAAAQKNGVPVITV
CCCCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEE
DRSINGGGVTAHLGRDNVKMGKAVGDAVLAELKKKGTTRGKVIEIQGDAGGTVMAARRDG
ECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCC
FHDALKGSGFKIVAGPYAEYTRANAVTAMQDLLQAHPDVKVVYAHNDDMAMGALKVLAEA
CHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHC
GKKDVFVAGVDGLSEALASMAGGDQYVATALNDPRYLGDMTVQTARAIKAGKKVPAFVDA
CCCCEEEECCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEECC
GTKAVTKANVGEFKRTGLFAEYRPAVFK
CCHHHHHCCCCHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]