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The map label for this gene is pcrA [H]

Identifier: 121609570

GI number: 121609570

Start: 2955019

End: 2955759

Strand: Direct

Name: pcrA [H]

Synonym: Veis_2615

Alternate gene names: 121609570

Gene position: 2955019-2955759 (Clockwise)

Preceding gene: 121609569

Following gene: 121609571

Centisome position: 53.08

GC content: 61.4

Gene sequence:

>741_bases
ATGACAACGAGGGAGGAACGGAATGACCAGCGCCGCCGCCCTCCAATCCAAGGACAATCCGCAGCAGCTCGACGCCATCC
TCACCACAGAAGGCCCGGTGCTCATCATCGCGGGCCCGGCTCCGGTAAGACCTTTACGCTGGTCGAGCGCATCGTCTATC
TCATCACCCAGAAAGGCGTCGCGCCGGAGTCTCTGTTTGTCGTCACCTTCACCGACAAGGCCGCGCACGAGCTGACCACG
CGCATCTCGAACCGCCTGACCGAGCTGGACATCAAGTTCAACCTGAACGAGATGTACCTCGGCACCTTCCACTCCATCTG
CCTGCGGCTGCTGGAGGAGTACCGCGAGTTCACGCGGCTCAAGCGCAGCTTCACGCTGTTCGACCAATTCGACCAGCAGT
ACTTCCTATACCAGCACATCAAGGACTTCCGCGAATTGCCCGACGCGCAGCTCGTCATGGGCGACGACCAGTCAGGGCGC
TGGGCGCAGTCGGAGAACCTGCTCAAGTGGCTCAACAAGATCAGCGAGGATGCCCTCGACGCTGCCACGCTCACAGCGGC
TCCCGAAGTGGAAATCCGTGCGCTGGCCGCGTGCTTCGCCAAGTATCAGGAACTGCTGCACGAGAACAACCCGCTCGACT
TCTCCGGCATCCAGTACGAGGCGCTGCAACTGCTGGAAAAGCGCCCCGAGGTGCTGGCACAGGTGCGCGAGAAACTCACC
TACCTGATGGTGGAGCCCTAA

Upstream 100 bases:

>100_bases
AGCGGGAAGCTCCCAGCCTTCAAACTCGGCGGAACGTGGCGCTTCCGGCGTGGCGAACTGGATCAGTGGATCGCCAGCCA
CATCGGCAAGGCATCGGTCG

Downstream 100 bases:

>100_bases
CGGAAATCGAACGGGCATCTACAACCCCGTAGGAATGTAGCGGAAATGGGCGGAAGCGCAGCCGCCGCGCCAAGCGGCAC
CGTTGTTCCTTCGTTGTTCA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTTREERNDQRRRPPIQGQSAAARRHPHHRRPGAHHRGPGSGKTFTLVERIVYLITQKGVAPESLFVVTFTDKAAHELTT
RISNRLTELDIKFNLNEMYLGTFHSICLRLLEEYREFTRLKRSFTLFDQFDQQYFLYQHIKDFRELPDAQLVMGDDQSGR
WAQSENLLKWLNKISEDALDAATLTAAPEVEIRALAACFAKYQELLHENNPLDFSGIQYEALQLLEKRPEVLAQVREKLT
YLMVEP

Sequences:

>Translated_246_residues
MTTREERNDQRRRPPIQGQSAAARRHPHHRRPGAHHRGPGSGKTFTLVERIVYLITQKGVAPESLFVVTFTDKAAHELTT
RISNRLTELDIKFNLNEMYLGTFHSICLRLLEEYREFTRLKRSFTLFDQFDQQYFLYQHIKDFRELPDAQLVMGDDQSGR
WAQSENLLKWLNKISEDALDAATLTAAPEVEIRALAACFAKYQELLHENNPLDFSGIQYEALQLLEKRPEVLAQVREKLT
YLMVEP
>Mature_245_residues
TTREERNDQRRRPPIQGQSAAARRHPHHRRPGAHHRGPGSGKTFTLVERIVYLITQKGVAPESLFVVTFTDKAAHELTTR
ISNRLTELDIKFNLNEMYLGTFHSICLRLLEEYREFTRLKRSFTLFDQFDQQYFLYQHIKDFRELPDAQLVMGDDQSGRW
AQSENLLKWLNKISEDALDAATLTAAPEVEIRALAACFAKYQELLHENNPLDFSGIQYEALQLLEKRPEVLAQVREKLTY
LMVEP

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=212, Percent_Identity=27.3584905660377, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI48994965, Length=208, Percent_Identity=27.4038461538462, Blast_Score=71, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322369, Length=222, Percent_Identity=28.8288288288288, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 28657; Mature: 28526

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTREERNDQRRRPPIQGQSAAARRHPHHRRPGAHHRGPGSGKTFTLVERIVYLITQKGV
CCCCHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
APESLFVVTFTDKAAHELTTRISNRLTELDIKFNLNEMYLGTFHSICLRLLEEYREFTRL
CCCCEEEEEECCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KRSFTLFDQFDQQYFLYQHIKDFRELPDAQLVMGDDQSGRWAQSENLLKWLNKISEDALD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AATLTAAPEVEIRALAACFAKYQELLHENNPLDFSGIQYEALQLLEKRPEVLAQVREKLT
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHH
YLMVEP
HHCCCC
>Mature Secondary Structure 
TTREERNDQRRRPPIQGQSAAARRHPHHRRPGAHHRGPGSGKTFTLVERIVYLITQKGV
CCCHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
APESLFVVTFTDKAAHELTTRISNRLTELDIKFNLNEMYLGTFHSICLRLLEEYREFTRL
CCCCEEEEEECCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KRSFTLFDQFDQQYFLYQHIKDFRELPDAQLVMGDDQSGRWAQSENLLKWLNKISEDALD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AATLTAAPEVEIRALAACFAKYQELLHENNPLDFSGIQYEALQLLEKRPEVLAQVREKLT
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHH
YLMVEP
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]