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The map label for this gene is nccB [H]

Identifier: 120613294

GI number: 120613294

Start: 5202214

End: 5203398

Strand: Direct

Name: nccB [H]

Synonym: Aave_4663

Alternate gene names: 120613294

Gene position: 5202214-5203398 (Clockwise)

Preceding gene: 120613293

Following gene: 120613295

Centisome position: 97.19

GC content: 74.85

Gene sequence:

>1185_bases
ATGCTGCGACTGCGTTCACGCCCCTCCCTGCTGGCCCTGTCGGCCGCCTGCCTCGCCGCTGCCGCCGGCGGCGCCCTCGT
GCTGGCCACACCCGGCGCCTCCCGTGCCGAAGCCGCCAGGGACAAGGACGCCGCGGCGACCGCGCCCCGGCCGGCGCTCA
CCGTGACCACCACCACGCCCCGGCGCGAATCGCTCGCCGCGCGGCTTCCTGCCAACGGCAACGTCGCCGCGTGGCAGGAA
GCGAGCATCGGCACCGAGAGCAACGGTTTGCGGCTGACCGAGGTACGGGTGAACGTGGGCGACACCGTGCGCGCGGGCCA
GGTGCTCGCAGTCTTCTCCGCCGACTCGGTGCAGGCCGAGCTGGCCCAGAGCCGCGCCAGCCTGCTGGAAGCGCGCGCGA
GCGCCGCCGAGGCCGCGGCCAACGCCGAGCGCGCCCGCACGCTGCAGGCCTCGGGCGCCCTGAGCCAGCAACAGATCCAG
CAATACACCACCGCCAGCGAAACCGCCGCCGCGCGCGTGGAGGCCGCGCAGGCCGCGCTCTCCGCGCAGCAACTGCGCCT
GAAGCACACCCAGGTGCTCGCACCCGATAGCGGCGTCATTTCCGCGCGCACGGCCACCGTGGGCTCGGTGGTGGGCGCCG
GTACCGAACTGTTCCGGATGGTGCGCAAGGGCCGCCTCGAGTGGCGCGCCGAAGTCACCTCCAGCGAACTGCCGCGCGTG
CGGCCCGGCATCCGCGCCCGCGTCACCGCGGCGAGCGGCGCCGAAGTGGAAGGCACCGTGCGCGTGGTGGCACCCACGGT
GGACCCGCAGTCGCGCAACGCCCTCGTCTATGTCGATCTGCCCGCCCATCCCGACATCCGGGCCGGCATGTTCGCGCGCG
GCGATTTCCTGCTCGCGCAGCGCGACACCCTCACCGTCCCGCAGTCGGCCGTGGTGGTGCGCGACGGCTTCACGGCCGTG
TTCGAGGTCGGCGAGGGCGGCCGCGTGGCGATGCGCCAGGTGAAGACCGGCCAGCGCACGGGCGACCGGGTGGAGATCCT
CTCGGGACTGCAGGAGCGCGCCACCCTCGTGGAACGCGGCGGCGCGTTCCTCAACGACGGCGACACCGTGCGCGTCGAGG
CATCCGGGGCGGCCGCGCCCGCCGCCGCCGCATCGACCGCGAAGAGCGCCGGCGCCGCGCGATAG

Upstream 100 bases:

>100_bases
GGACCCGCGCAGGCATCCACACCCGTACCCGCCTCCGCCGCGCCCTGACGGCCCGCCCCGCATCCCCGACACACCGACCG
CAACTTCCGGACCCTTTTCC

Downstream 100 bases:

>100_bases
AGAAGAAGCTGTCCCCCCGCCCATGCCGCATCCGCATTCCCGCCAGAGCCCCGAGAGCCCGCTGCCATGAACCTTTCCGC
CTGGTCCATCCGCAACCCCA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLRLRSRPSLLALSAACLAAAAGGALVLATPGASRAEAARDKDAAATAPRPALTVTTTTPRRESLAARLPANGNVAAWQE
ASIGTESNGLRLTEVRVNVGDTVRAGQVLAVFSADSVQAELAQSRASLLEARASAAEAAANAERARTLQASGALSQQQIQ
QYTTASETAAARVEAAQAALSAQQLRLKHTQVLAPDSGVISARTATVGSVVGAGTELFRMVRKGRLEWRAEVTSSELPRV
RPGIRARVTAASGAEVEGTVRVVAPTVDPQSRNALVYVDLPAHPDIRAGMFARGDFLLAQRDTLTVPQSAVVVRDGFTAV
FEVGEGGRVAMRQVKTGQRTGDRVEILSGLQERATLVERGGAFLNDGDTVRVEASGAAAPAAAASTAKSAGAAR

Sequences:

>Translated_394_residues
MLRLRSRPSLLALSAACLAAAAGGALVLATPGASRAEAARDKDAAATAPRPALTVTTTTPRRESLAARLPANGNVAAWQE
ASIGTESNGLRLTEVRVNVGDTVRAGQVLAVFSADSVQAELAQSRASLLEARASAAEAAANAERARTLQASGALSQQQIQ
QYTTASETAAARVEAAQAALSAQQLRLKHTQVLAPDSGVISARTATVGSVVGAGTELFRMVRKGRLEWRAEVTSSELPRV
RPGIRARVTAASGAEVEGTVRVVAPTVDPQSRNALVYVDLPAHPDIRAGMFARGDFLLAQRDTLTVPQSAVVVRDGFTAV
FEVGEGGRVAMRQVKTGQRTGDRVEILSGLQERATLVERGGAFLNDGDTVRVEASGAAAPAAAASTAKSAGAAR
>Mature_394_residues
MLRLRSRPSLLALSAACLAAAAGGALVLATPGASRAEAARDKDAAATAPRPALTVTTTTPRRESLAARLPANGNVAAWQE
ASIGTESNGLRLTEVRVNVGDTVRAGQVLAVFSADSVQAELAQSRASLLEARASAAEAAANAERARTLQASGALSQQQIQ
QYTTASETAAARVEAAQAALSAQQLRLKHTQVLAPDSGVISARTATVGSVVGAGTELFRMVRKGRLEWRAEVTSSELPRV
RPGIRARVTAASGAEVEGTVRVVAPTVDPQSRNALVYVDLPAHPDIRAGMFARGDFLLAQRDTLTVPQSAVVVRDGFTAV
FEVGEGGRVAMRQVKTGQRTGDRVEILSGLQERATLVERGGAFLNDGDTVRVEASGAAAPAAAASTAKSAGAAR

Specific function: Component of the NCC cation efflux system that confers resistance to nickel, cobalt and cadmium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=331, Percent_Identity=26.5861027190332, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789929, Length=335, Percent_Identity=25.0746268656716, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40667; Mature: 40667

Theoretical pI: Translated: 11.14; Mature: 11.14

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLRSRPSLLALSAACLAAAAGGALVLATPGASRAEAARDKDAAATAPRPALTVTTTTP
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCCCCCEEEEEECCC
RRESLAARLPANGNVAAWQEASIGTESNGLRLTEVRVNVGDTVRAGQVLAVFSADSVQAE
CHHHHHEECCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCHHHHH
LAQSRASLLEARASAAEAAANAERARTLQASGALSQQQIQQYTTASETAAARVEAAQAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SAQQLRLKHTQVLAPDSGVISARTATVGSVVGAGTELFRMVRKGRLEWRAEVTSSELPRV
HHHHHHHHHHEEECCCCCEEEECHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCC
RPGIRARVTAASGAEVEGTVRVVAPTVDPQSRNALVYVDLPAHPDIRAGMFARGDFLLAQ
CCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEECCCEEEEE
RDTLTVPQSAVVVRDGFTAVFEVGEGGRVAMRQVKTGQRTGDRVEILSGLQERATLVERG
CCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
GAFLNDGDTVRVEASGAAAPAAAASTAKSAGAAR
CCEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLRLRSRPSLLALSAACLAAAAGGALVLATPGASRAEAARDKDAAATAPRPALTVTTTTP
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCCCCCEEEEEECCC
RRESLAARLPANGNVAAWQEASIGTESNGLRLTEVRVNVGDTVRAGQVLAVFSADSVQAE
CHHHHHEECCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCHHHHH
LAQSRASLLEARASAAEAAANAERARTLQASGALSQQQIQQYTTASETAAARVEAAQAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SAQQLRLKHTQVLAPDSGVISARTATVGSVVGAGTELFRMVRKGRLEWRAEVTSSELPRV
HHHHHHHHHHEEECCCCCEEEECHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCC
RPGIRARVTAASGAEVEGTVRVVAPTVDPQSRNALVYVDLPAHPDIRAGMFARGDFLLAQ
CCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEECCCEEEEE
RDTLTVPQSAVVVRDGFTAVFEVGEGGRVAMRQVKTGQRTGDRVEILSGLQERATLVERG
CCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
GAFLNDGDTVRVEASGAAAPAAAASTAKSAGAAR
CCEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961470 [H]