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The map label for this gene is 120613175

Identifier: 120613175

GI number: 120613175

Start: 5058091

End: 5059386

Strand: Reverse

Name: 120613175

Synonym: Aave_4542

Alternate gene names: NA

Gene position: 5059386-5058091 (Counterclockwise)

Preceding gene: 120613176

Following gene: 120613174

Centisome position: 94.52

GC content: 65.66

Gene sequence:

>1296_bases
ATGGTTCCGCATCTCATCACCGCCCTCACGGGGCCGATCAACGAACTAGAACAACGCATCCTCGACGCCATGCCCGCGAT
CGAGCGCTGGTTCCGGCTCGAGTGGATGGAGCACACCCCGCCCTTCTACACCTCGGTGGACATCCGCAACGCCGGCTTCA
AGCTCGCGCCCGTGGACACCAACCTCTTCCCCGGAGGCTGGAACAACCTCACGGCCGAGATGCTCCCGCTGGCCGTGCAG
GCGGCCATGGCCGCGATCGAGAAGATCTGCCCCGAAGCGCGCAACCTCCTCATCGTCCCGGAGAACCACAGCCGCAACAC
GTTCTACCTCGCGAACGTGATCCAGCTGCAGCGCATCTTCAACATGGCCGGGCTCAACGTGCGCGTGGGCTCCATCAGCC
CCGAGATCAAGAAGCCGACCACCGTCACGCTGCCCAACGGCGACTCGGTCACGCTGGAGCCGGTGGTGCGCGACAAGGGC
CGCCTCGGGCTCAAGAACTTCGACCCCTGCACCATCCTGCTCAACAACGACCTGTCCGCCGGCCCTCCGGGCATCCTGGA
GGACCTGCATGAGCAGTACCTGCTGCCGCCCCTGCATGCCGGCTGGACCGTACGCCGCAAGAGCCGGCACTTCCAGAGCT
ACGAGGAGGTCTCCAAGCGCTTCGGCAAGCTCCTGGGCATCGACCCCTGGCTCATCCATCCGCTCTTCAGCCACTGCGAA
GGGCTGGATTTCAGCGAAGGCGCCGGCGTGGAGGCCCTGCGCTCCGCCGTGGATGCGCAACTGACCAAGGTCCGGCGCAA
GTACAAGGAATACGGCATCAACGAAAAGCCGTTCGTCATCGTCAAGGCCGACAACGGCACCTACGGCATGGGCGTCATGA
CCGTGCGCGACGCCAAGGACCTCGACGCGCTCAACCGCAAGACGCGCAACAAGATGGCCATCGTCAAGGACGGCCAGCCG
GTGACCGACGTCATCATCCAGGAGGGCGTCCTGACCCGCGAGCGCGTGCACGAGGCCGTCGCCGAGCCCGTCGTTTACAT
GATGGACCGCTACGTCGTGGGAGGCTTCTACCGCATGCATGCCGAGCGCGGCGTGGACGAGAACCTCAATGCGCCCGGGG
CGAGCTTCGTGCCGCTCGCCTTCGAGCAAAGCACCCATCTCCCCCAGCCCGGCGCCAGGCCCGGCGCCAGTGCGCCCAAC
CGGTTCTACATGTATGGCGTGATCGCCCGGCTGGCCATGCTGGCGGCCAGCTACGAACTCGAGGCGACCAACCCCGAGGC
CGAGATCTACGAATAA

Upstream 100 bases:

>100_bases
GCGGCGCGGAGTGCTTGCGAACCGGGCCCGCGCCCCCAAATCCCTACAATCGACTCCGCTTTCGGCGCAATGCCGTCTTC
GCCGCTTTCCGAGGGTACCC

Downstream 100 bases:

>100_bases
GCCTCTGCGCAGCGGCGCGCCCGTCCTGCTTAGTTGCTGCTCTGCAACATGGAGCGGGCGGCCGGAGCGCTTTCCCTGCT
CCTTGTCACAATAGCGGTCC

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVPHLITALTGPINELEQRILDAMPAIERWFRLEWMEHTPPFYTSVDIRNAGFKLAPVDTNLFPGGWNNLTAEMLPLAVQ
AAMAAIEKICPEARNLLIVPENHSRNTFYLANVIQLQRIFNMAGLNVRVGSISPEIKKPTTVTLPNGDSVTLEPVVRDKG
RLGLKNFDPCTILLNNDLSAGPPGILEDLHEQYLLPPLHAGWTVRRKSRHFQSYEEVSKRFGKLLGIDPWLIHPLFSHCE
GLDFSEGAGVEALRSAVDAQLTKVRRKYKEYGINEKPFVIVKADNGTYGMGVMTVRDAKDLDALNRKTRNKMAIVKDGQP
VTDVIIQEGVLTRERVHEAVAEPVVYMMDRYVVGGFYRMHAERGVDENLNAPGASFVPLAFEQSTHLPQPGARPGASAPN
RFYMYGVIARLAMLAASYELEATNPEAEIYE

Sequences:

>Translated_431_residues
MVPHLITALTGPINELEQRILDAMPAIERWFRLEWMEHTPPFYTSVDIRNAGFKLAPVDTNLFPGGWNNLTAEMLPLAVQ
AAMAAIEKICPEARNLLIVPENHSRNTFYLANVIQLQRIFNMAGLNVRVGSISPEIKKPTTVTLPNGDSVTLEPVVRDKG
RLGLKNFDPCTILLNNDLSAGPPGILEDLHEQYLLPPLHAGWTVRRKSRHFQSYEEVSKRFGKLLGIDPWLIHPLFSHCE
GLDFSEGAGVEALRSAVDAQLTKVRRKYKEYGINEKPFVIVKADNGTYGMGVMTVRDAKDLDALNRKTRNKMAIVKDGQP
VTDVIIQEGVLTRERVHEAVAEPVVYMMDRYVVGGFYRMHAERGVDENLNAPGASFVPLAFEQSTHLPQPGARPGASAPN
RFYMYGVIARLAMLAASYELEATNPEAEIYE
>Mature_431_residues
MVPHLITALTGPINELEQRILDAMPAIERWFRLEWMEHTPPFYTSVDIRNAGFKLAPVDTNLFPGGWNNLTAEMLPLAVQ
AAMAAIEKICPEARNLLIVPENHSRNTFYLANVIQLQRIFNMAGLNVRVGSISPEIKKPTTVTLPNGDSVTLEPVVRDKG
RLGLKNFDPCTILLNNDLSAGPPGILEDLHEQYLLPPLHAGWTVRRKSRHFQSYEEVSKRFGKLLGIDPWLIHPLFSHCE
GLDFSEGAGVEALRSAVDAQLTKVRRKYKEYGINEKPFVIVKADNGTYGMGVMTVRDAKDLDALNRKTRNKMAIVKDGQP
VTDVIIQEGVLTRERVHEAVAEPVVYMMDRYVVGGFYRMHAERGVDENLNAPGASFVPLAFEQSTHLPQPGARPGASAPN
RFYMYGVIARLAMLAASYELEATNPEAEIYE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 48126; Mature: 48126

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLITALTGPINELEQRILDAMPAIERWFRLEWMEHTPPFYTSVDIRNAGFKLAPVDT
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCC
NLFPGGWNNLTAEMLPLAVQAAMAAIEKICPEARNLLIVPENHSRNTFYLANVIQLQRIF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCEEEEECCCCCCEEEHHHHHHHHHHH
NMAGLNVRVGSISPEIKKPTTVTLPNGDSVTLEPVVRDKGRLGLKNFDPCTILLNNDLSA
HHCCCEEEECCCCCCCCCCEEEECCCCCCEEEEHHHCCCCCCCCCCCCCEEEEECCCCCC
GPPGILEDLHEQYLLPPLHAGWTVRRKSRHFQSYEEVSKRFGKLLGIDPWLIHPLFSHCE
CCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
GLDFSEGAGVEALRSAVDAQLTKVRRKYKEYGINEKPFVIVKADNGTYGMGVMTVRDAKD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCHH
LDALNRKTRNKMAIVKDGQPVTDVIIQEGVLTRERVHEAVAEPVVYMMDRYVVGGFYRMH
HHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERGVDENLNAPGASFVPLAFEQSTHLPQPGARPGASAPNRFYMYGVIARLAMLAASYEL
HHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEE
EATNPEAEIYE
ECCCCCCCCCC
>Mature Secondary Structure
MVPHLITALTGPINELEQRILDAMPAIERWFRLEWMEHTPPFYTSVDIRNAGFKLAPVDT
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCC
NLFPGGWNNLTAEMLPLAVQAAMAAIEKICPEARNLLIVPENHSRNTFYLANVIQLQRIF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCEEEEECCCCCCEEEHHHHHHHHHHH
NMAGLNVRVGSISPEIKKPTTVTLPNGDSVTLEPVVRDKGRLGLKNFDPCTILLNNDLSA
HHCCCEEEECCCCCCCCCCEEEECCCCCCEEEEHHHCCCCCCCCCCCCCEEEEECCCCCC
GPPGILEDLHEQYLLPPLHAGWTVRRKSRHFQSYEEVSKRFGKLLGIDPWLIHPLFSHCE
CCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
GLDFSEGAGVEALRSAVDAQLTKVRRKYKEYGINEKPFVIVKADNGTYGMGVMTVRDAKD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCHH
LDALNRKTRNKMAIVKDGQPVTDVIIQEGVLTRERVHEAVAEPVVYMMDRYVVGGFYRMH
HHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERGVDENLNAPGASFVPLAFEQSTHLPQPGARPGASAPNRFYMYGVIARLAMLAASYEL
HHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCEE
EATNPEAEIYE
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA