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The map label for this gene is gshB [H]

Identifier: 120613172

GI number: 120613172

Start: 5053821

End: 5054771

Strand: Reverse

Name: gshB [H]

Synonym: Aave_4539

Alternate gene names: 120613172

Gene position: 5054771-5053821 (Counterclockwise)

Preceding gene: 120613173

Following gene: 120613171

Centisome position: 94.43

GC content: 63.62

Gene sequence:

>951_bases
ATGAACATTCTTTTCGTTGCCGATCCGGTCGAACACTTCAAGATCTACAAGGACACCACGTTCTCGATGATGCGGGAGGC
CCAGCGCCGGGGGCATGTGGTGAGCGTTTGCGAGGCCGGCCATATCCGCTGGGAGCAGGGGAGCAAGGTGACGGCGCGCG
TACGCGACCTCCACCTCACGGGTGACGCGCAGGCCTGGTTCACCGTGGCAGGTGAGCGTGAGGCCGAGCTCTCCGGATTC
GGCGCCGTGCTCATGCGCAAGGATCCTCCCTTCGACAGCGAGTACTTCTATGCCACCCATCTCCTCGAGCAGGCGGAGCG
GGAGGGGGCGAGGGTCTTCAACAAGCCCCGCGCGCTGCGCGACCATCCCGAGAAGCTGGCCATCATGGAGTTTCCCGACC
TCATCGGGCCTACGCTCGTCACGCGGGATGCGGGGGACATCCGGCGCTTCCACGCGGAACATGGGGACATCATCCTCAAG
CCGCTGGACGGGATGGGCGGCATGGGCATCTTCCGGGTCAAGGCCGACGGACTCAATCTGGGCAGCATCATCGAAACCCT
CAACCACCATGGCGCGCAGACCGTCATGGTGCAGAAGTTCCTTCCCGAGATATCCGAAGGAGACAAGCGCGTCCTCATCA
TCGGAGGGGAGCCCGTGCCTTATTGCCTCGCACGCATCCCCCAGGGATCGGAAGTCCGTGGCAACCTTGCGGCTGGCGGC
AAAGGAGTGGCTCGCCCGCTCTCGGCCCGCGATGTCGAGATCGGTCGCCGGCTCGGCCCCGTGCTGCTGGAGCGGGGCAT
GCTGCTGGCGGGCGTGGACGTCATCGGGAACTGCGTCACCGAAATCAACGTGACGAGCCCCACCTGTTTCCAGGAGATCT
TCGATCAGACGGCATGCGACGTCCCCTCCCTGTTTGTCGATGCGCTGGAAAAACAATTGAAATTGCGCTGA

Upstream 100 bases:

>100_bases
CCTTCTGGGGCGTGGTCGCCGGCGTGCTCACGCTATTTGTGCAACAGTACGGACGCCGCCCCCGCGGCGCCTGACGCCAC
CTTCCCAGCCAGACGAGACC

Downstream 100 bases:

>100_bases
GGCCACTTTTTGTTGCTATACTAGCGGGCTTCGCTACTGAGATTGCTGCGGTTCCTGAGAGGGCTGCGATGAAGGTAGGT
GGCGAAGGCGGTAGAAGAGA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNILFVADPVEHFKIYKDTTFSMMREAQRRGHVVSVCEAGHIRWEQGSKVTARVRDLHLTGDAQAWFTVAGEREAELSGF
GAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNKPRALRDHPEKLAIMEFPDLIGPTLVTRDAGDIRRFHAEHGDIILK
PLDGMGGMGIFRVKADGLNLGSIIETLNHHGAQTVMVQKFLPEISEGDKRVLIIGGEPVPYCLARIPQGSEVRGNLAAGG
KGVARPLSARDVEIGRRLGPVLLERGMLLAGVDVIGNCVTEINVTSPTCFQEIFDQTACDVPSLFVDALEKQLKLR

Sequences:

>Translated_316_residues
MNILFVADPVEHFKIYKDTTFSMMREAQRRGHVVSVCEAGHIRWEQGSKVTARVRDLHLTGDAQAWFTVAGEREAELSGF
GAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNKPRALRDHPEKLAIMEFPDLIGPTLVTRDAGDIRRFHAEHGDIILK
PLDGMGGMGIFRVKADGLNLGSIIETLNHHGAQTVMVQKFLPEISEGDKRVLIIGGEPVPYCLARIPQGSEVRGNLAAGG
KGVARPLSARDVEIGRRLGPVLLERGMLLAGVDVIGNCVTEINVTSPTCFQEIFDQTACDVPSLFVDALEKQLKLR
>Mature_316_residues
MNILFVADPVEHFKIYKDTTFSMMREAQRRGHVVSVCEAGHIRWEQGSKVTARVRDLHLTGDAQAWFTVAGEREAELSGF
GAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNKPRALRDHPEKLAIMEFPDLIGPTLVTRDAGDIRRFHAEHGDIILK
PLDGMGGMGIFRVKADGLNLGSIIETLNHHGAQTVMVQKFLPEISEGDKRVLIIGGEPVPYCLARIPQGSEVRGNLAAGG
KGVARPLSARDVEIGRRLGPVLLERGMLLAGVDVIGNCVTEINVTSPTCFQEIFDQTACDVPSLFVDALEKQLKLR

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=310, Percent_Identity=49.6774193548387, Blast_Score=322, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34922; Mature: 34922

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILFVADPVEHFKIYKDTTFSMMREAQRRGHVVSVCEAGHIRWEQGSKVTARVRDLHLT
CEEEEEECCHHHHEECCCHHHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEEEEEEEC
GDAQAWFTVAGEREAELSGFGAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNKPRALR
CCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
DHPEKLAIMEFPDLIGPTLVTRDAGDIRRFHAEHGDIILKPLDGMGGMGIFRVKADGLNL
CCCHHEEEECCCHHHCCEEEECCCHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCH
GSIIETLNHHGAQTVMVQKFLPEISEGDKRVLIIGGEPVPYCLARIPQGSEVRGNLAAGG
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCC
KGVARPLSARDVEIGRRLGPVLLERGMLLAGVDVIGNCVTEINVTSPTCFQEIFDQTACD
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHEEECCCCHHHHHHHHHHHCC
VPSLFVDALEKQLKLR
CHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNILFVADPVEHFKIYKDTTFSMMREAQRRGHVVSVCEAGHIRWEQGSKVTARVRDLHLT
CEEEEEECCHHHHEECCCHHHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEEEEEEEC
GDAQAWFTVAGEREAELSGFGAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNKPRALR
CCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
DHPEKLAIMEFPDLIGPTLVTRDAGDIRRFHAEHGDIILKPLDGMGGMGIFRVKADGLNL
CCCHHEEEECCCHHHCCEEEECCCHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCH
GSIIETLNHHGAQTVMVQKFLPEISEGDKRVLIIGGEPVPYCLARIPQGSEVRGNLAAGG
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCC
KGVARPLSARDVEIGRRLGPVLLERGMLLAGVDVIGNCVTEINVTSPTCFQEIFDQTACD
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHEEECCCCHHHHHHHHHHHCC
VPSLFVDALEKQLKLR
CHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]