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The map label for this gene is rep [H]

Identifier: 120613110

GI number: 120613110

Start: 4974048

End: 4976117

Strand: Direct

Name: rep [H]

Synonym: Aave_4475

Alternate gene names: 120613110

Gene position: 4974048-4976117 (Clockwise)

Preceding gene: 120613106

Following gene: 120613111

Centisome position: 92.92

GC content: 67.34

Gene sequence:

>2070_bases
ATGTCCGCCGGTCTCAATCTCGCCCAGCTCCAGGCCGTCCACTACACGGAGGGGCCGTGCCTCGTGCTGGCCGGCGCCGG
CTCGGGCAAGACGCGCGTGATCACGATGAAGATCGGCCGGCTGATCGAGACCGGCCTGGAGCCGCGCCGCATCGCGGCCA
TCACCTTCACGAACAAGGCCGCCGCCGAAATGCGCGAGCGCGCGCAGCACCTGATCGGCCGCGCGGCCAAAGATGTTCTG
GTCTGCACCTTCCATGCCCTGGGCGTGCGCATGGTGCGGGAAGACGGGCACGTGCTGGGCCTCAAGCCCCAGTTCAGCAT
CCTCGACGCCGACGACGTGACCGGCATCATCAAGGACGCGGCCGGCGGCACCACCGACATCGCCACCGCGCGCCAGTGGC
AATGGACCATCAGCGCCTGGAAGAACGCCGGCCTGGACAGCGCCCAGGCCCTCGCGCAGGCGAAGGACGACAACGAGCGC
AGCATCGCCGTCATCATGCAGCGCTACGAGGAGCGCCTGACGGCCTACCAGAGCGTGGACTTCGACGACCTGATCGGCAT
GCCGCTGCGCCTGCTGCGCGACTTCCCCGAGGTGCGCGCGAAGTGGCAGCAGTCGCTGGGCCACGTGCTGGTCGATGAAT
ACCAGGACACCAACGCCACGCAGTACGAACTGCTCAAGCTGCTGGTGGGAGAGCGCGGCCGTTTCACCGCCGTGGGCGAC
GACGACCAGTCGATCTACGGCTGGCGCGGCGCCACGCTGGACAACCTCAAGAAGCTGCCGGTGGACTACCCGCAGCTCAA
GGTCATCAAGCTGGAGCAGAACTACCGCTCCACCAGCGCCATCCTGCGCGCGGCCAACAACGTCATCCAGCCCAACCCCA
AGCTCTTTCCGAAAACGCTGTTCTCCGAGCTGGGCGAGGGCGAGCCGGTGCGCGTGGTGGACGCCGACAACGAGGAGCAC
GAGGCCGAGCGCGCCGTCGCCCGCATCCAGAGCCTGCGCGCCACGCTGAACCCGCAGCCGGCGTGGAAGAGCTTCGCCAT
CCTCTACCGCGCCAACCACCAGGCCAAGCCGTTCGAGAAGGCGCTGCGCAAGGCCAACGTGCCCTACAAGGTCTCGGGCG
GCACCAGTTTCTTCGACCGCGCGGAAATCAAGGACCTGTGCGCCTGGTTCCGCCTGTGGATCAACAACGACGACGACCCG
GCCTTCCTGCGCGCCATCACCACGCCCAAGCGCGGCATCGGCCACACCACGCTGGCCAAGCTCGGCGAGTTCTCGACGCA
GCACAAGGTGAGCATGTTCGGCGCGCTCTTCAACCACATGCTCGAGGCCGTGCTGCCGCGCAAGGCGCACGAGAGCGTGA
TGGAGTTCGGCCGCTACATCAACTACCTGGAATACAAGGCGCGGCACACCCATGGCGCCGAGGACGCGCGCACCTTCATG
ACCGACTGGCTGAAGGAGATCGGCTACGAGCAGTACCTCTACGACAGCGAGGACAGCGAAACGGTGGCCGGCGCACGCTG
GACCAACGTGCTCGAATTCTGCGACTGGATGAGCCAGCGCGCTGGCGGGCAGATCGACGACACCGCCGGCGCCGTGGTCG
CGAAGGAAACCAAGAGCCTGCTGGAAGTGGCGCAGACCATCGCGCTGCTGTCCACCATCAGCGAGCGCGAGAAGGACGAA
GACCTCGTCACCCTCTCGACCCTGCACGCCTCCAAGGGCCTGGAATGGCCGCACGTGATGCTGGTGGGCGTGACCGAGGG
CATGCTGCCCTTCAAGCTCGACGACGACGAAGGCCGCCAGCAGAAGGTGAGCGAAGACACCCTGCAGCGCCTGCAGGAAG
AGCGCCGCCTGATGTACGTGGGCATCACCCGCGCGCAGCGCAGCCTGGCCGTGAGCTGGACCAAGCGCCGCAAGAAAGGC
CGCGAGATGGTCGCCGCCCAGCCCAGCCGCTTCATCGCCGAGATGGGCCTGGACAAGAACACCGCCCGCGAAGACCCGCG
CGAGAAGCTGCGCGCGCTGCGTGCCGAGTTCGCGGCACGCAAGGCCCAGCCTGCCCCGACGGCACCATGA

Upstream 100 bases:

>100_bases
CCCGCCTTCCCCGGTCCGGGCCGCTTTCCGCCGTCCGCTGGTGGAACGCGTCCCCCTGCCCGCCCTTTCGCGCTCGCCTT
TTTTCCGCTCCGCTCGCTGC

Downstream 100 bases:

>100_bases
ACCCGCCGGACACGCCATCCACCCCGGGCCGGCTCCGCGCCCGGGCTCGCGGCGAGCGGCACGCAGAAACCCATCATCAT
CCACGCCACCCGGATGGGCC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MSAGLNLAQLQAVHYTEGPCLVLAGAGSGKTRVITMKIGRLIETGLEPRRIAAITFTNKAAAEMRERAQHLIGRAAKDVL
VCTFHALGVRMVREDGHVLGLKPQFSILDADDVTGIIKDAAGGTTDIATARQWQWTISAWKNAGLDSAQALAQAKDDNER
SIAVIMQRYEERLTAYQSVDFDDLIGMPLRLLRDFPEVRAKWQQSLGHVLVDEYQDTNATQYELLKLLVGERGRFTAVGD
DDQSIYGWRGATLDNLKKLPVDYPQLKVIKLEQNYRSTSAILRAANNVIQPNPKLFPKTLFSELGEGEPVRVVDADNEEH
EAERAVARIQSLRATLNPQPAWKSFAILYRANHQAKPFEKALRKANVPYKVSGGTSFFDRAEIKDLCAWFRLWINNDDDP
AFLRAITTPKRGIGHTTLAKLGEFSTQHKVSMFGALFNHMLEAVLPRKAHESVMEFGRYINYLEYKARHTHGAEDARTFM
TDWLKEIGYEQYLYDSEDSETVAGARWTNVLEFCDWMSQRAGGQIDDTAGAVVAKETKSLLEVAQTIALLSTISEREKDE
DLVTLSTLHASKGLEWPHVMLVGVTEGMLPFKLDDDEGRQQKVSEDTLQRLQEERRLMYVGITRAQRSLAVSWTKRRKKG
REMVAAQPSRFIAEMGLDKNTAREDPREKLRALRAEFAARKAQPAPTAP

Sequences:

>Translated_689_residues
MSAGLNLAQLQAVHYTEGPCLVLAGAGSGKTRVITMKIGRLIETGLEPRRIAAITFTNKAAAEMRERAQHLIGRAAKDVL
VCTFHALGVRMVREDGHVLGLKPQFSILDADDVTGIIKDAAGGTTDIATARQWQWTISAWKNAGLDSAQALAQAKDDNER
SIAVIMQRYEERLTAYQSVDFDDLIGMPLRLLRDFPEVRAKWQQSLGHVLVDEYQDTNATQYELLKLLVGERGRFTAVGD
DDQSIYGWRGATLDNLKKLPVDYPQLKVIKLEQNYRSTSAILRAANNVIQPNPKLFPKTLFSELGEGEPVRVVDADNEEH
EAERAVARIQSLRATLNPQPAWKSFAILYRANHQAKPFEKALRKANVPYKVSGGTSFFDRAEIKDLCAWFRLWINNDDDP
AFLRAITTPKRGIGHTTLAKLGEFSTQHKVSMFGALFNHMLEAVLPRKAHESVMEFGRYINYLEYKARHTHGAEDARTFM
TDWLKEIGYEQYLYDSEDSETVAGARWTNVLEFCDWMSQRAGGQIDDTAGAVVAKETKSLLEVAQTIALLSTISEREKDE
DLVTLSTLHASKGLEWPHVMLVGVTEGMLPFKLDDDEGRQQKVSEDTLQRLQEERRLMYVGITRAQRSLAVSWTKRRKKG
REMVAAQPSRFIAEMGLDKNTAREDPREKLRALRAEFAARKAQPAPTAP
>Mature_688_residues
SAGLNLAQLQAVHYTEGPCLVLAGAGSGKTRVITMKIGRLIETGLEPRRIAAITFTNKAAAEMRERAQHLIGRAAKDVLV
CTFHALGVRMVREDGHVLGLKPQFSILDADDVTGIIKDAAGGTTDIATARQWQWTISAWKNAGLDSAQALAQAKDDNERS
IAVIMQRYEERLTAYQSVDFDDLIGMPLRLLRDFPEVRAKWQQSLGHVLVDEYQDTNATQYELLKLLVGERGRFTAVGDD
DQSIYGWRGATLDNLKKLPVDYPQLKVIKLEQNYRSTSAILRAANNVIQPNPKLFPKTLFSELGEGEPVRVVDADNEEHE
AERAVARIQSLRATLNPQPAWKSFAILYRANHQAKPFEKALRKANVPYKVSGGTSFFDRAEIKDLCAWFRLWINNDDDPA
FLRAITTPKRGIGHTTLAKLGEFSTQHKVSMFGALFNHMLEAVLPRKAHESVMEFGRYINYLEYKARHTHGAEDARTFMT
DWLKEIGYEQYLYDSEDSETVAGARWTNVLEFCDWMSQRAGGQIDDTAGAVVAKETKSLLEVAQTIALLSTISEREKDED
LVTLSTLHASKGLEWPHVMLVGVTEGMLPFKLDDDEGRQQKVSEDTLQRLQEERRLMYVGITRAQRSLAVSWTKRRKKGR
EMVAAQPSRFIAEMGLDKNTAREDPREKLRALRAEFAARKAQPAPTAP

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=661, Percent_Identity=45.5370650529501, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI2367296, Length=655, Percent_Identity=36.1832061068702, Blast_Score=357, Evalue=2e-99,
Organism=Escherichia coli, GI1787196, Length=346, Percent_Identity=28.3236994219653, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=730, Percent_Identity=27.9452054794521, Blast_Score=194, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77397; Mature: 77266

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGLNLAQLQAVHYTEGPCLVLAGAGSGKTRVITMKIGRLIETGLEPRRIAAITFTNKA
CCCCCCHHHHEEEEECCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCCCEEEEEEECCHH
AAEMRERAQHLIGRAAKDVLVCTFHALGVRMVREDGHVLGLKPQFSILDADDVTGIIKDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCEEECCHHHHHHHHHC
AGGTTDIATARQWQWTISAWKNAGLDSAQALAQAKDDNERSIAVIMQRYEERLTAYQSVD
CCCCCCCCCCHHHEEEHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
FDDLIGMPLRLLRDFPEVRAKWQQSLGHVLVDEYQDTNATQYELLKLLVGERGRFTAVGD
HHHHHHHHHHHHHHCHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECC
DDQSIYGWRGATLDNLKKLPVDYPQLKVIKLEQNYRSTSAILRAANNVIQPNPKLFPKTL
CCCCEECCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHH
FSELGEGEPVRVVDADNEEHEAERAVARIQSLRATLNPQPAWKSFAILYRANHQAKPFEK
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCCHHHH
ALRKANVPYKVSGGTSFFDRAEIKDLCAWFRLWINNDDDPAFLRAITTPKRGIGHTTLAK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCHHHHHH
LGEFSTQHKVSMFGALFNHMLEAVLPRKAHESVMEFGRYINYLEYKARHTHGAEDARTFM
HHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TDWLKEIGYEQYLYDSEDSETVAGARWTNVLEFCDWMSQRAGGQIDDTAGAVVAKETKSL
HHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LEVAQTIALLSTISEREKDEDLVTLSTLHASKGLEWPHVMLVGVTEGMLPFKLDDDEGRQ
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCHH
QKVSEDTLQRLQEERRLMYVGITRAQRSLAVSWTKRRKKGREMVAAQPSRFIAEMGLDKN
HHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
TAREDPREKLRALRAEFAARKAQPAPTAP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SAGLNLAQLQAVHYTEGPCLVLAGAGSGKTRVITMKIGRLIETGLEPRRIAAITFTNKA
CCCCCHHHHEEEEECCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCCCEEEEEEECCHH
AAEMRERAQHLIGRAAKDVLVCTFHALGVRMVREDGHVLGLKPQFSILDADDVTGIIKDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCEEECCHHHHHHHHHC
AGGTTDIATARQWQWTISAWKNAGLDSAQALAQAKDDNERSIAVIMQRYEERLTAYQSVD
CCCCCCCCCCHHHEEEHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
FDDLIGMPLRLLRDFPEVRAKWQQSLGHVLVDEYQDTNATQYELLKLLVGERGRFTAVGD
HHHHHHHHHHHHHHCHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECC
DDQSIYGWRGATLDNLKKLPVDYPQLKVIKLEQNYRSTSAILRAANNVIQPNPKLFPKTL
CCCCEECCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHH
FSELGEGEPVRVVDADNEEHEAERAVARIQSLRATLNPQPAWKSFAILYRANHQAKPFEK
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCCHHHH
ALRKANVPYKVSGGTSFFDRAEIKDLCAWFRLWINNDDDPAFLRAITTPKRGIGHTTLAK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCHHHHHH
LGEFSTQHKVSMFGALFNHMLEAVLPRKAHESVMEFGRYINYLEYKARHTHGAEDARTFM
HHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TDWLKEIGYEQYLYDSEDSETVAGARWTNVLEFCDWMSQRAGGQIDDTAGAVVAKETKSL
HHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LEVAQTIALLSTISEREKDEDLVTLSTLHASKGLEWPHVMLVGVTEGMLPFKLDDDEGRQ
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCHH
QKVSEDTLQRLQEERRLMYVGITRAQRSLAVSWTKRRKKGREMVAAQPSRFIAEMGLDKN
HHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
TAREDPREKLRALRAEFAARKAQPAPTAP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]