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The map label for this gene is priA [H]

Identifier: 120613106

GI number: 120613106

Start: 4967646

End: 4969937

Strand: Direct

Name: priA [H]

Synonym: Aave_4471

Alternate gene names: 120613106

Gene position: 4967646-4969937 (Clockwise)

Preceding gene: 120613105

Following gene: 120613110

Centisome position: 92.81

GC content: 74.69

Gene sequence:

>2292_bases
ATGTACCGGGATGCCCACAAAGTTATCCACACCCCGGCCCGAAAACCTTCCGTGCACCCGGCGGCCGCTTGCGGCAGACT
CGGGCGCCGCCCTGCACTTGCCTCCGTCCCTTCCGTGTCCCAACCCCTGTCCCGCGTCCCGTCCGATGCATCGCCACCCG
CCGCAACCGACGGCGGCGCGATCGTCTCGGTCGCCGTCCAGACGCCCGCGCACAGCGCGGTGGGCGGCCCGCTGAGCTAC
GCGAGCGCGCATCCGCTGGAGCCGGGCACGCTGGTGCGGGTGCCCCTGGGCACGCGCGAAGTGCTGGGCGTGGTGTGGGA
TGCGCCGGCCGATGCGCCGGAGCTGCCGCGCGGCGCCGCGGTGCGGCCGGTGGCGGGCGTGCTGGAGGGGCTGGCGCCGC
TGGACGCCGCCTGGCGGCGGCTGGTGGCCTTCGCGGCGCGCTACTACCAGCGGGCGTTGGGCGAGGTGGCGCTCGCCGCC
CTGCCGCCGCAGTTGCGCGACCTCACGCCCGCGCAGCTCGCGCGGCGCCTGCGCCGCGCGCCGGCCGCCACCGCCGGGGA
AGCCGCGCCGCCCTATGCACCCGAGCCCCACGCCCTGAGCGCCGAACAGCAGGCGGCCACGGACGCCATCGCCGCCCGGC
CCGGACCCTTCCTGCTCTACGGCAGCACGGGCAGCGGCAAGACCGAGGTGTACCTGCGCTGCGTGCAGCAGGCCCTGGCC
GCGGATCCCTCCGCCCAGGTGCTGGTGATGGTGCCGGAGATCAACCTCACGCCGCAGCTGGAGGCGCGGTTCCTCGCCCG
CTTCGCGCCACTCTACGGGGCGGACAGCGTGGTGTCGCTGCACAGCGGCATGACCCACCCGCAGCGGCTGAAAAGCTGGC
TGGCCGCGCATGCCGGCCAGGCGCGCATCGTGCTGGGCACGCGCATGGCGGTGTTCGCGAGCCTGCCGGGCCTGGCGCTC
ATCGTGGTGGACGAGGAGCACGACGCGAGCTACAAGCAGCAGGAAGGCGCGCGCTACTCGGCGCGCGACCTGGCCATCTG
GCGCGGGCGCGAGCAGGACGCGAAGGTGATCCTCGGCTCGGCCACCCCGTCACTGGAGAGCTGGCACGCGAGCCGCCCGC
CCACGGACGGTGACCCCGGCGGGCGCTACCTGCGGCTGCACATGCCCAGCCGGATCGGCGCGGGCGCGCTACCGCGCGTG
CGGCTGGTGGACATGGCGCAGCAGCCGCGCGGCGCCGTGTTCTCGCCGCCGCTCGTGGCCGCCATCACCGAGCGCGTGGA
GCGCGGCGAACAGTGCCTGGTGCTGCTCAACCGCCGCGGCTTCGCCCCGGTGCTGCACTGCACGGAGTGCGGCTGGAAGA
GCGACTGCCCGCACTGCAGCGCCCACCAGGTGTTCCACAAGATCGACCGCTCGCTGCGCTGCCACCACTGCGGCTTCGCG
CAGCGCGTGCCCTACGCCTGCCCCGGCTGCGGCAACCCGGACATCGCGCCGATCGGGCGCGGCACCGAGCAACTGGAAGA
GCAGTTGGCCGCGCTGCTGCGCAACGTGATCACGCCGGAGCGCCGCGCCGCGCGCGTGGCCCGCATCGACGCCGACACCA
CGAAAGCCAAGGGCGCGCTGGAAGAACAGCTCGCCCAGGTGCATGCGGGCGAGGTGGACGTGCTGGTGGGCACGCAGATG
GTCGCCAAGGGACACGATTTCCGGCGCATCACGCTGGTGGCGGCGGTACAGCCGGACGGCGCGCTGTTCTCCAGCGATTT
CCGGGCGCCCGAGCGGCTGTTCGCGCTGCTCATGCAGGCGGCGGGCCGTGCGGGCCGGGACGCGGGCTACGTGGCCGCCC
AGGGTGGCGCCTGCGAGATGTGGGTGCAGACCTACCACCCCGACCATGCGGTGTACGCGGCGCTGCGCCGGCACGACTAT
CCGGCCTTCGCCGCGCGGCAGCTCGCCGAGCGCGCCGAGGCCGCCATGCCGCCCTTCGCATTCCAGGCGCTGGTGCGTGC
CGACGCGCGCACGCAGGAGGTGGCGCAGACCTTCCTGGCCGCCTGCGCCGAGGCGGCCCGGCAGGCGGGCCTTCCGGGGC
TGGAGCAGGTGACGGTGTTCCCGCCCGTTCCCCTGGCGATCCAGCGCGTGGCCAACGTGGAGCGCGCGCAGATGCTGCTG
GAGAGCCCGAGCCGCGCGGCGCTGCAGCGCTTCCTGGCGGCCTGGCAGCCGCTGCTGCATGCCTGCCGCGCGGACCCGGC
GCACCGGGGGCTGGTGCGGTGGCTGGTGGACGTGGATCCGCTGGCGATCTGA

Upstream 100 bases:

>100_bases
ACAGTTCGCCCAACAGCATTGATTTAAAAGGCTTTTTTCTTCACCCTGGGAACAGCGGAACACCATCGCGCGCGGCAGCT
GTGCACCGCCTGCCCCCTGG

Downstream 100 bases:

>100_bases
GCGGGCCGCGGCCGGCCGGCCGAGGATCAGTCGGCGTGCGGCCGGGGCCCTTCCCGCCAGGCGATGAGCGCCTCGCGCTG
CTCCAGGTGGCGCACCACGC

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 763; Mature: 763

Protein sequence:

>763_residues
MYRDAHKVIHTPARKPSVHPAAACGRLGRRPALASVPSVSQPLSRVPSDASPPAATDGGAIVSVAVQTPAHSAVGGPLSY
ASAHPLEPGTLVRVPLGTREVLGVVWDAPADAPELPRGAAVRPVAGVLEGLAPLDAAWRRLVAFAARYYQRALGEVALAA
LPPQLRDLTPAQLARRLRRAPAATAGEAAPPYAPEPHALSAEQQAATDAIAARPGPFLLYGSTGSGKTEVYLRCVQQALA
ADPSAQVLVMVPEINLTPQLEARFLARFAPLYGADSVVSLHSGMTHPQRLKSWLAAHAGQARIVLGTRMAVFASLPGLAL
IVVDEEHDASYKQQEGARYSARDLAIWRGREQDAKVILGSATPSLESWHASRPPTDGDPGGRYLRLHMPSRIGAGALPRV
RLVDMAQQPRGAVFSPPLVAAITERVERGEQCLVLLNRRGFAPVLHCTECGWKSDCPHCSAHQVFHKIDRSLRCHHCGFA
QRVPYACPGCGNPDIAPIGRGTEQLEEQLAALLRNVITPERRAARVARIDADTTKAKGALEEQLAQVHAGEVDVLVGTQM
VAKGHDFRRITLVAAVQPDGALFSSDFRAPERLFALLMQAAGRAGRDAGYVAAQGGACEMWVQTYHPDHAVYAALRRHDY
PAFAARQLAERAEAAMPPFAFQALVRADARTQEVAQTFLAACAEAARQAGLPGLEQVTVFPPVPLAIQRVANVERAQMLL
ESPSRAALQRFLAAWQPLLHACRADPAHRGLVRWLVDVDPLAI

Sequences:

>Translated_763_residues
MYRDAHKVIHTPARKPSVHPAAACGRLGRRPALASVPSVSQPLSRVPSDASPPAATDGGAIVSVAVQTPAHSAVGGPLSY
ASAHPLEPGTLVRVPLGTREVLGVVWDAPADAPELPRGAAVRPVAGVLEGLAPLDAAWRRLVAFAARYYQRALGEVALAA
LPPQLRDLTPAQLARRLRRAPAATAGEAAPPYAPEPHALSAEQQAATDAIAARPGPFLLYGSTGSGKTEVYLRCVQQALA
ADPSAQVLVMVPEINLTPQLEARFLARFAPLYGADSVVSLHSGMTHPQRLKSWLAAHAGQARIVLGTRMAVFASLPGLAL
IVVDEEHDASYKQQEGARYSARDLAIWRGREQDAKVILGSATPSLESWHASRPPTDGDPGGRYLRLHMPSRIGAGALPRV
RLVDMAQQPRGAVFSPPLVAAITERVERGEQCLVLLNRRGFAPVLHCTECGWKSDCPHCSAHQVFHKIDRSLRCHHCGFA
QRVPYACPGCGNPDIAPIGRGTEQLEEQLAALLRNVITPERRAARVARIDADTTKAKGALEEQLAQVHAGEVDVLVGTQM
VAKGHDFRRITLVAAVQPDGALFSSDFRAPERLFALLMQAAGRAGRDAGYVAAQGGACEMWVQTYHPDHAVYAALRRHDY
PAFAARQLAERAEAAMPPFAFQALVRADARTQEVAQTFLAACAEAARQAGLPGLEQVTVFPPVPLAIQRVANVERAQMLL
ESPSRAALQRFLAAWQPLLHACRADPAHRGLVRWLVDVDPLAI
>Mature_763_residues
MYRDAHKVIHTPARKPSVHPAAACGRLGRRPALASVPSVSQPLSRVPSDASPPAATDGGAIVSVAVQTPAHSAVGGPLSY
ASAHPLEPGTLVRVPLGTREVLGVVWDAPADAPELPRGAAVRPVAGVLEGLAPLDAAWRRLVAFAARYYQRALGEVALAA
LPPQLRDLTPAQLARRLRRAPAATAGEAAPPYAPEPHALSAEQQAATDAIAARPGPFLLYGSTGSGKTEVYLRCVQQALA
ADPSAQVLVMVPEINLTPQLEARFLARFAPLYGADSVVSLHSGMTHPQRLKSWLAAHAGQARIVLGTRMAVFASLPGLAL
IVVDEEHDASYKQQEGARYSARDLAIWRGREQDAKVILGSATPSLESWHASRPPTDGDPGGRYLRLHMPSRIGAGALPRV
RLVDMAQQPRGAVFSPPLVAAITERVERGEQCLVLLNRRGFAPVLHCTECGWKSDCPHCSAHQVFHKIDRSLRCHHCGFA
QRVPYACPGCGNPDIAPIGRGTEQLEEQLAALLRNVITPERRAARVARIDADTTKAKGALEEQLAQVHAGEVDVLVGTQM
VAKGHDFRRITLVAAVQPDGALFSSDFRAPERLFALLMQAAGRAGRDAGYVAAQGGACEMWVQTYHPDHAVYAALRRHDY
PAFAARQLAERAEAAMPPFAFQALVRADARTQEVAQTFLAACAEAARQAGLPGLEQVTVFPPVPLAIQRVANVERAQMLL
ESPSRAALQRFLAAWQPLLHACRADPAHRGLVRWLVDVDPLAI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=568, Percent_Identity=42.7816901408451, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81692; Mature: 81692

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRDAHKVIHTPARKPSVHPAAACGRLGRRPALASVPSVSQPLSRVPSDASPPAATDGGA
CCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCE
IVSVAVQTPAHSAVGGPLSYASAHPLEPGTLVRVPLGTREVLGVVWDAPADAPELPRGAA
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCC
VRPVAGVLEGLAPLDAAWRRLVAFAARYYQRALGEVALAALPPQLRDLTPAQLARRLRRA
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHC
PAATAGEAAPPYAPEPHALSAEQQAATDAIAARPGPFLLYGSTGSGKTEVYLRCVQQALA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHH
ADPSAQVLVMVPEINLTPQLEARFLARFAPLYGADSVVSLHSGMTHPQRLKSWLAAHAGQ
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHCCCC
ARIVLGTRMAVFASLPGLALIVVDEEHDASYKQQEGARYSARDLAIWRGREQDAKVILGS
EEEEEEHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHCCCCCCCCEEEEEC
ATPSLESWHASRPPTDGDPGGRYLRLHMPSRIGAGALPRVRLVDMAQQPRGAVFSPPLVA
CCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHH
AITERVERGEQCLVLLNRRGFAPVLHCTECGWKSDCPHCSAHQVFHKIDRSLRCHHCGFA
HHHHHHHCCCCEEEEECCCCCCCEEEHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCHH
QRVPYACPGCGNPDIAPIGRGTEQLEEQLAALLRNVITPERRAARVARIDADTTKAKGAL
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
EEQLAQVHAGEVDVLVGTQMVAKGHDFRRITLVAAVQPDGALFSSDFRAPERLFALLMQA
HHHHHHHHCCCEEEEECCHHHHCCCCCEEEEEEEEECCCCCEECCCCCCHHHHHHHHHHH
AGRAGRDAGYVAAQGGACEMWVQTYHPDHAVYAALRRHDYPAFAARQLAERAEAAMPPFA
HCCCCCCCCEEEECCCCEEEHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHH
FQALVRADARTQEVAQTFLAACAEAARQAGLPGLEQVTVFPPVPLAIQRVANVERAQMLL
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
ESPSRAALQRFLAAWQPLLHACRADPAHRGLVRWLVDVDPLAI
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYRDAHKVIHTPARKPSVHPAAACGRLGRRPALASVPSVSQPLSRVPSDASPPAATDGGA
CCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCE
IVSVAVQTPAHSAVGGPLSYASAHPLEPGTLVRVPLGTREVLGVVWDAPADAPELPRGAA
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCC
VRPVAGVLEGLAPLDAAWRRLVAFAARYYQRALGEVALAALPPQLRDLTPAQLARRLRRA
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHC
PAATAGEAAPPYAPEPHALSAEQQAATDAIAARPGPFLLYGSTGSGKTEVYLRCVQQALA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHH
ADPSAQVLVMVPEINLTPQLEARFLARFAPLYGADSVVSLHSGMTHPQRLKSWLAAHAGQ
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHCCCC
ARIVLGTRMAVFASLPGLALIVVDEEHDASYKQQEGARYSARDLAIWRGREQDAKVILGS
EEEEEEHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHCCCCCCCCEEEEEC
ATPSLESWHASRPPTDGDPGGRYLRLHMPSRIGAGALPRVRLVDMAQQPRGAVFSPPLVA
CCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHH
AITERVERGEQCLVLLNRRGFAPVLHCTECGWKSDCPHCSAHQVFHKIDRSLRCHHCGFA
HHHHHHHCCCCEEEEECCCCCCCEEEHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCHH
QRVPYACPGCGNPDIAPIGRGTEQLEEQLAALLRNVITPERRAARVARIDADTTKAKGAL
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
EEQLAQVHAGEVDVLVGTQMVAKGHDFRRITLVAAVQPDGALFSSDFRAPERLFALLMQA
HHHHHHHHCCCEEEEECCHHHHCCCCCEEEEEEEEECCCCCEECCCCCCHHHHHHHHHHH
AGRAGRDAGYVAAQGGACEMWVQTYHPDHAVYAALRRHDYPAFAARQLAERAEAAMPPFA
HCCCCCCCCEEEECCCCEEEHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHH
FQALVRADARTQEVAQTFLAACAEAARQAGLPGLEQVTVFPPVPLAIQRVANVERAQMLL
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
ESPSRAALQRFLAAWQPLLHACRADPAHRGLVRWLVDVDPLAI
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]