Click here to switch to the map view.

The map label for this gene is fliA [H]

Identifier: 120613052

GI number: 120613052

Start: 4911213

End: 4911929

Strand: Direct

Name: fliA [H]

Synonym: Aave_4416

Alternate gene names: 120613052

Gene position: 4911213-4911929 (Clockwise)

Preceding gene: 120613051

Following gene: 120613056

Centisome position: 91.75

GC content: 65.97

Gene sequence:

>717_bases
ATGTACACCGCCAAAGGACAGCTCAACCGCGATGCATTGATCCGCCAGCACGCGCCGCTGGTCCGCCGGATCGCGCACCA
CATGATCGCGAAGCTTCCGCCCAACGTGGAGCTGGACGATCTGATCCAGGTCGGCATGATCGGGCTCAACGAGGCGCTGT
CGCGCTACGAGGTGACGCAGGGCGTGCAGTTCGAGACTTTCGCCTCCCAGCGCATCCGCGGCGCCATGCTCGACGAGCTG
CGCGAGGGCGACTGGATGTCGCGCAGTTCGCGCAAGAGCCAGAAGGAGATCGAGCACGCCGTGACCCGGCTGGAGCAGAA
GCTCGGCCGCAGCCCGCACGAATCCGAGATCGCCACGGAGCTCGAACTCAGCCTGGCGGAATACCAGTCCCTGCTGAGCA
AGGTGCGCGGTACCCAGCTGGTTTACCTCGAAGACATGACGCGCGGCGACGACGACGATGACGGTTTCCTCGACCGCCAC
GTGGCCGACAGCGCGGCCGATCCCATGGCCATGCTGCGCGACCAGCGCCTGCGCGCCTCCCTGGTGGCGGCGATCAAGGC
GCTGCCCGAACGGGAGCAGCAGATCATGGGCATGTACTACGAGCACGACATGAACCTGAAGGAGATCGCCGCGGTGCTGG
GCGTGACCGAATCGCGCATCTGCCAGCTGCACAGCCAGTCGATCGCCCGGCTGCGCGCGAAGATGCGCAGCCATTGA

Upstream 100 bases:

>100_bases
CTGGAAAACGCGGGCACCATTGGCGCCGCGCCGGCCGCAGCCGCCCCGCCCTTCGGTACGCAGCGGTCCGCCCAGCCCTT
CGCACGGAGCCACTGATCCG

Downstream 100 bases:

>100_bases
CGCTGCCAGCGCCCGGCATGCCGGTCGTCCGGGAAAGCAATAAGAAAGGGCCCCTCGGGGCCCTTGGTTTTTTGCGGCAC
AGCCCGGCAGGGCCGCACGG

Product: FliA/WhiG family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MYTAKGQLNRDALIRQHAPLVRRIAHHMIAKLPPNVELDDLIQVGMIGLNEALSRYEVTQGVQFETFASQRIRGAMLDEL
REGDWMSRSSRKSQKEIEHAVTRLEQKLGRSPHESEIATELELSLAEYQSLLSKVRGTQLVYLEDMTRGDDDDDGFLDRH
VADSAADPMAMLRDQRLRASLVAAIKALPEREQQIMGMYYEHDMNLKEIAAVLGVTESRICQLHSQSIARLRAKMRSH

Sequences:

>Translated_238_residues
MYTAKGQLNRDALIRQHAPLVRRIAHHMIAKLPPNVELDDLIQVGMIGLNEALSRYEVTQGVQFETFASQRIRGAMLDEL
REGDWMSRSSRKSQKEIEHAVTRLEQKLGRSPHESEIATELELSLAEYQSLLSKVRGTQLVYLEDMTRGDDDDDGFLDRH
VADSAADPMAMLRDQRLRASLVAAIKALPEREQQIMGMYYEHDMNLKEIAAVLGVTESRICQLHSQSIARLRAKMRSH
>Mature_238_residues
MYTAKGQLNRDALIRQHAPLVRRIAHHMIAKLPPNVELDDLIQVGMIGLNEALSRYEVTQGVQFETFASQRIRGAMLDEL
REGDWMSRSSRKSQKEIEHAVTRLEQKLGRSPHESEIATELELSLAEYQSLLSKVRGTQLVYLEDMTRGDDDDDGFLDRH
VADSAADPMAMLRDQRLRASLVAAIKALPEREQQIMGMYYEHDMNLKEIAAVLGVTESRICQLHSQSIARLRAKMRSH

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=236, Percent_Identity=44.9152542372881, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1789448, Length=210, Percent_Identity=27.6190476190476, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27105; Mature: 27105

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTAKGQLNRDALIRQHAPLVRRIAHHMIAKLPPNVELDDLIQVGMIGLNEALSRYEVTQ
CCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GVQFETFASQRIRGAMLDELREGDWMSRSSRKSQKEIEHAVTRLEQKLGRSPHESEIATE
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LELSLAEYQSLLSKVRGTQLVYLEDMTRGDDDDDGFLDRHVADSAADPMAMLRDQRLRAS
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LVAAIKALPEREQQIMGMYYEHDMNLKEIAAVLGVTESRICQLHSQSIARLRAKMRSH
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYTAKGQLNRDALIRQHAPLVRRIAHHMIAKLPPNVELDDLIQVGMIGLNEALSRYEVTQ
CCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GVQFETFASQRIRGAMLDELREGDWMSRSSRKSQKEIEHAVTRLEQKLGRSPHESEIATE
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LELSLAEYQSLLSKVRGTQLVYLEDMTRGDDDDDGFLDRHVADSAADPMAMLRDQRLRAS
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LVAAIKALPEREQQIMGMYYEHDMNLKEIAAVLGVTESRICQLHSQSIARLRAKMRSH
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]