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The map label for this gene is leuA

Identifier: 120611768

GI number: 120611768

Start: 3432018

End: 3433556

Strand: Reverse

Name: leuA

Synonym: Aave_3108

Alternate gene names: 120611768

Gene position: 3433556-3432018 (Counterclockwise)

Preceding gene: 120611769

Following gene: 120611767

Centisome position: 64.15

GC content: 66.99

Gene sequence:

>1539_bases
ATGTCCGACAAACTCATCATCTTCGACACCACCCTGCGCGACGGCGAGCAGTCTCCCGGCGCCTCGATGACCCGCGACGA
GAAGCTGCGCATCGCCCGCCAGCTCGAGCGCCTCAGGGTGGACGTGATCGAGGCCGGCTTCGCCGCCAGCTCGAACGGGG
ATTTCGAGGCCGTGCAGGCGATCGCCCAGGCCATCAAGGATTCGACGGTGTGCTCGCTGTCCCGCGCGAACGACCGCGAC
ATCGCCCGCGCGGCGGAGGCGCTCAAGGACGCGCAGCGCGCGCGCATCCACACCTTCATCGCCACCAGCCCGCTGCACAT
GGAAAAGAAGCTTCGCATGACGCCGGAGCAGGTGCTGGAGCAGGCGAAGCAGTCCGTGCGTTTCGCGCGCAATCTCGTGG
CCGACATCGAATTCAGCCCGGAAGACGGCTACCGCAGCGAGCCCGACTTCCTCTGCCGCGTGCTGGAGGCCGTGATCGCC
GAGGGGGCCACGACGATCAACGTGCCGGACACGGTGGGCTATGCCATTCCCGAGCTCTACGGCAATTTCATCAGGAACCT
GCGCGAGCGCATCCCCAACAGCGACAAGGCCGTTTGGTCGGTGCATTGCCACAACGACCTCGGCATGGCGGTGGCCAACT
CCCTGGCCGGCGTGAAGATCGGCGGCGCCCGTCAGGTCGAATGCACCATCAATGGCCTGGGCGAGCGGGCGGGCAACTGC
TCGCTGGAGGAGATCGTCATGGCCGTGAAGACGCGGCGCGACTACTTCGGCCTGGAACTGGGCATCGATACGCAGCACAT
CGTGGCCGCCAGCCGCATGGTGAGCCAGACCACGGGCTTCGTCGTGCAGCCGAACAAGGCCGTGGTGGGCGCCAACGCCT
TCGCCCATGCCAGCGGCATCCACCAGGACGGCGTGCTCAAGGCCCGCGATACCTACGAGATCATGCGCGCGGAGGACGTG
GGCTGGACCGCCAACAAGATCGTGCTGGGCAAGCTGAGCGGGCGCAACGCCTTCAAGCAGCGGCTGCAGGACCTCGGCGT
GCAGCTGGAGAGCGAGGCGGACGTGAACGCCGCTTTCCTGCGCTTCAAGGAACTCGCGGACCGCAAGAGCGAGATCTTCG
ACGAGGACATCCTCGCGCTGGTCAGCGGCGACGCCACGGCCGGCGCCGGCGAGCGCTACGGCTTCGTGTCCCTGTCGCAG
CGCAGCGAGACCGGCGAGCGGCCCCATGCCGCGGTGGTCTTCACCGTGGACGGCAAGGAGGTGCATGGCGCATCGGAAGG
CAACGGCCCGGTCGATGCATCGCTCAAGGCCATCGAGTCGCATGTGCACAGCGGCGCCGAGATGGTGCTGTATTCAGTGA
ACGCGATCAGCGGCTCCACGGAGAGCCAGGGCGAGGTGACCGTGCGCCTGCAGGACAGCGGGCGGGTCGTCAACGGGGTG
GGGGCGGACCCGGACATCGTCGTCGCGTCAGCCAAAGCCTACCTGAGCGCGTTGAACAAATTGCAAAGCAAAGCCGAGCG
CGTGGCTGCGCAAGGCTGA

Upstream 100 bases:

>100_bases
CCGACGAAGGCCCGCATGCACATGGCAGCGGGCCTTTTTCTTGCGGGCCCTCGCGCCCTGCGGGGCGGGGCGCCGGCCCA
CCAACGACAAGGAGCACACG

Downstream 100 bases:

>100_bases
AACTGGAATTTATAATTCAGCCACTTCATTGAGAAGGTCGCGCAAGATGTTGATCTTGCGCGACTTTTTTTGCTCACATA
CACTGTCACACAATTTTGCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLRVDVIEAGFAASSNGDFEAVQAIAQAIKDSTVCSLSRANDRD
IARAAEALKDAQRARIHTFIATSPLHMEKKLRMTPEQVLEQAKQSVRFARNLVADIEFSPEDGYRSEPDFLCRVLEAVIA
EGATTINVPDTVGYAIPELYGNFIRNLRERIPNSDKAVWSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNC
SLEEIVMAVKTRRDYFGLELGIDTQHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQDLGVQLESEADVNAAFLRFKELADRKSEIFDEDILALVSGDATAGAGERYGFVSLSQ
RSETGERPHAAVVFTVDGKEVHGASEGNGPVDASLKAIESHVHSGAEMVLYSVNAISGSTESQGEVTVRLQDSGRVVNGV
GADPDIVVASAKAYLSALNKLQSKAERVAAQG

Sequences:

>Translated_512_residues
MSDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLRVDVIEAGFAASSNGDFEAVQAIAQAIKDSTVCSLSRANDRD
IARAAEALKDAQRARIHTFIATSPLHMEKKLRMTPEQVLEQAKQSVRFARNLVADIEFSPEDGYRSEPDFLCRVLEAVIA
EGATTINVPDTVGYAIPELYGNFIRNLRERIPNSDKAVWSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNC
SLEEIVMAVKTRRDYFGLELGIDTQHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQDLGVQLESEADVNAAFLRFKELADRKSEIFDEDILALVSGDATAGAGERYGFVSLSQ
RSETGERPHAAVVFTVDGKEVHGASEGNGPVDASLKAIESHVHSGAEMVLYSVNAISGSTESQGEVTVRLQDSGRVVNGV
GADPDIVVASAKAYLSALNKLQSKAERVAAQG
>Mature_511_residues
SDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLRVDVIEAGFAASSNGDFEAVQAIAQAIKDSTVCSLSRANDRDI
ARAAEALKDAQRARIHTFIATSPLHMEKKLRMTPEQVLEQAKQSVRFARNLVADIEFSPEDGYRSEPDFLCRVLEAVIAE
GATTINVPDTVGYAIPELYGNFIRNLRERIPNSDKAVWSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNCS
LEEIVMAVKTRRDYFGLELGIDTQHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WTANKIVLGKLSGRNAFKQRLQDLGVQLESEADVNAAFLRFKELADRKSEIFDEDILALVSGDATAGAGERYGFVSLSQR
SETGERPHAAVVFTVDGKEVHGASEGNGPVDASLKAIESHVHSGAEMVLYSVNAISGSTESQGEVTVRLQDSGRVVNGVG
ADPDIVVASAKAYLSALNKLQSKAERVAAQG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=507, Percent_Identity=48.3234714003945, Blast_Score=441, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6324682, Length=537, Percent_Identity=25.3258845437616, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=542, Percent_Identity=24.5387453874539, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320019, Length=374, Percent_Identity=27.5401069518717, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320071, Length=366, Percent_Identity=27.8688524590164, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_ACIAC (A1TRT4)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971446.1
- ProteinModelPortal:   A1TRT4
- SMR:   A1TRT4
- STRING:   A1TRT4
- GeneID:   4665612
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3108
- NMPDR:   fig|397945.5.peg.2648
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   A1TRT4
- ProtClustDB:   PRK00915
- BioCyc:   AAVE397945:AAVE_3108-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55194; Mature: 55063

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLRVDVIEAGFAASSNGDFEAVQA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IAQAIKDSTVCSLSRANDRDIARAAEALKDAQRARIHTFIATSPLHMEKKLRMTPEQVLE
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCHHHHHH
QAKQSVRFARNLVADIEFSPEDGYRSEPDFLCRVLEAVIAEGATTINVPDTVGYAIPELY
HHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
GNFIRNLRERIPNSDKAVWSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNC
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRRDYFGLELGIDTQHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQDLGVQLESEADVNAAFL
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHH
RFKELADRKSEIFDEDILALVSGDATAGAGERYGFVSLSQRSETGERPHAAVVFTVDGKE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCE
VHGASEGNGPVDASLKAIESHVHSGAEMVLYSVNAISGSTESQGEVTVRLQDSGRVVNGV
ECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEECC
GADPDIVVASAKAYLSALNKLQSKAERVAAQG
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLRVDVIEAGFAASSNGDFEAVQA
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IAQAIKDSTVCSLSRANDRDIARAAEALKDAQRARIHTFIATSPLHMEKKLRMTPEQVLE
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCHHHHHH
QAKQSVRFARNLVADIEFSPEDGYRSEPDFLCRVLEAVIAEGATTINVPDTVGYAIPELY
HHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
GNFIRNLRERIPNSDKAVWSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNC
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRRDYFGLELGIDTQHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQDLGVQLESEADVNAAFL
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHH
RFKELADRKSEIFDEDILALVSGDATAGAGERYGFVSLSQRSETGERPHAAVVFTVDGKE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCE
VHGASEGNGPVDASLKAIESHVHSGAEMVLYSVNAISGSTESQGEVTVRLQDSGRVVNGV
ECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEECC
GADPDIVVASAKAYLSALNKLQSKAERVAAQG
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA