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The map label for this gene is yiaJ [H]

Identifier: 120611766

GI number: 120611766

Start: 3429886

End: 3430698

Strand: Direct

Name: yiaJ [H]

Synonym: Aave_3106

Alternate gene names: 120611766

Gene position: 3429886-3430698 (Clockwise)

Preceding gene: 120611765

Following gene: 120611773

Centisome position: 64.08

GC content: 68.51

Gene sequence:

>813_bases
ATGAAAAAAGACGACGCAGGCGCCTCCTCCAAACCCAGTGTCCAGGTGCTGGAGCGCATGTTCACCCTCATCGACGTGCT
CGCCTCGCGGGAAGAAGCCATTCCCCTGAAGGAAATCAGCGAGAAGACGGGGCTGCATCCCTCCACCGCGCACCGCATCC
TCAACGACCTGTCCCTCGGCCGCTTCGTGGACCGGCCCGAATCGGGCAGCTACCGGCTCGGCATGCGGCTCCTGGAACTG
GGCAACCTCGTCAAGGCCCGGTTGAACGTGCGGGACGCCGCGCTCCTGCCCATGCGCAGCCTGCACAAGCTGACCCAGCA
GCCTGTCAACCTCAGCATGCGCCAGGGCGACGAGATCGTCTATGTCGAGCGGGCCTACAGCGAGCGCTCCGGCATGCAGG
TGGTCCGCGCCATAGGCGGCCGCGCGCCCCTGCACCTGACATCCACCGGCAAGCTGTTCCTGGCTGTGGACGATCCGCAG
CGCGTGCGCGCCTATGCCGTGCGCACCGGGCTGGCAGGCCACACGCGCAACAGCATCACCCAGTTGCCCCAGCTGGAGCG
TGAACTGGGCCAGGCCCGGCAGTACGGCATCGCCCGCGACAACGAGGAACTGGAACTGGGCGTGCGCTGCATGGCGGCCG
GCGTGTTCGACGACCAGGGCCAGCTCGTCGCCGGCCTGTCCATTTCCGCCCCGGCCGACCGCCTGGACGAGAACTGGCTG
CCCAAGCTGCAGGCCACCGCGGACGAGATCTCCGCGGCACTGGGCTATGACCGCGACCGGGCCCGGAAGGCCGCCGGCTC
GTCGCACCGGTAG

Upstream 100 bases:

>100_bases
ACTGCGGGTGCACGGCGAGTGGCAGCGCGGCACCTGAGCTTCCGCGCCGCGGCTGAATTTCATAGGATAAAATTCCCGGA
CATTGCCTTGGCCGAGAACC

Downstream 100 bases:

>100_bases
AGCCTCGATGACAAAGGCCATGCGCCGGTGAGCGCATGGCCTGGGGATGCCGGGAGTTTCCCGCCGTGCAAGGCGGAATC
CCGCGGGGGTCAGTCCAGCT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKKDDAGASSKPSVQVLERMFTLIDVLASREEAIPLKEISEKTGLHPSTAHRILNDLSLGRFVDRPESGSYRLGMRLLEL
GNLVKARLNVRDAALLPMRSLHKLTQQPVNLSMRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLAVDDPQ
RVRAYAVRTGLAGHTRNSITQLPQLERELGQARQYGIARDNEELELGVRCMAAGVFDDQGQLVAGLSISAPADRLDENWL
PKLQATADEISAALGYDRDRARKAAGSSHR

Sequences:

>Translated_270_residues
MKKDDAGASSKPSVQVLERMFTLIDVLASREEAIPLKEISEKTGLHPSTAHRILNDLSLGRFVDRPESGSYRLGMRLLEL
GNLVKARLNVRDAALLPMRSLHKLTQQPVNLSMRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLAVDDPQ
RVRAYAVRTGLAGHTRNSITQLPQLERELGQARQYGIARDNEELELGVRCMAAGVFDDQGQLVAGLSISAPADRLDENWL
PKLQATADEISAALGYDRDRARKAAGSSHR
>Mature_270_residues
MKKDDAGASSKPSVQVLERMFTLIDVLASREEAIPLKEISEKTGLHPSTAHRILNDLSLGRFVDRPESGSYRLGMRLLEL
GNLVKARLNVRDAALLPMRSLHKLTQQPVNLSMRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLAVDDPQ
RVRAYAVRTGLAGHTRNSITQLPQLERELGQARQYGIARDNEELELGVRCMAAGVFDDQGQLVAGLSISAPADRLDENWL
PKLQATADEISAALGYDRDRARKAAGSSHR

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=250, Percent_Identity=35.6, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786468, Length=255, Percent_Identity=32.9411764705882, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1788131, Length=249, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786715, Length=246, Percent_Identity=30.4878048780488, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082359, Length=257, Percent_Identity=28.7937743190661, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790752, Length=224, Percent_Identity=27.2321428571429, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788581, Length=250, Percent_Identity=28, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29745; Mature: 29745

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDDAGASSKPSVQVLERMFTLIDVLASREEAIPLKEISEKTGLHPSTAHRILNDLSLG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHH
RFVDRPESGSYRLGMRLLELGNLVKARLNVRDAALLPMRSLHKLTQQPVNLSMRQGDEIV
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCEE
YVERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLAVDDPQRVRAYAVRTGLAGHTRNSIT
EEEHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHH
QLPQLERELGQARQYGIARDNEELELGVRCMAAGVFDDQGQLVAGLSISAPADRLDENWL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHCCCCC
PKLQATADEISAALGYDRDRARKAAGSSHR
CHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKKDDAGASSKPSVQVLERMFTLIDVLASREEAIPLKEISEKTGLHPSTAHRILNDLSLG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHH
RFVDRPESGSYRLGMRLLELGNLVKARLNVRDAALLPMRSLHKLTQQPVNLSMRQGDEIV
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCEE
YVERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLAVDDPQRVRAYAVRTGLAGHTRNSIT
EEEHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHH
QLPQLERELGQARQYGIARDNEELELGVRCMAAGVFDDQGQLVAGLSISAPADRLDENWL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHCCCCC
PKLQATADEISAALGYDRDRARKAAGSSHR
CHHHHHHHHHHHHHCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]