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The map label for this gene is def2 [H]

Identifier: 120611762

GI number: 120611762

Start: 3426863

End: 3427465

Strand: Reverse

Name: def2 [H]

Synonym: Aave_3102

Alternate gene names: 120611762

Gene position: 3427465-3426863 (Counterclockwise)

Preceding gene: 120611767

Following gene: 120611759

Centisome position: 64.03

GC content: 69.98

Gene sequence:

>603_bases
ATGCTGCGCGTCCCCCCCGACCGGCGGCGATCCGCGCCGCCGCCCACTTCGCGAGCTTTCTACATGGCCATCCACGACAT
CCTCAAGATGGGCGACGAGCGTCTTCTGCGCACCGCCCGGCCCGTCGCCGAATTCGGCACGCCTTCGCTGCTGCAACTGG
TGCAGGACATGCGGGACACCATGCGCGCGGTGAACGGCGCGGGCCTCGCCGCGCCGCAGATCGGGGTGGACCTGCAGGTG
GTGATCTTCGGATCGGAGGAGCGCAATCCGCGCTACCCCGACCGGCCGCTGGTGCCGCCCACGGTGCTGGTGAACCCGCG
GATCACGCCGCTGGGCGGCGAGGAGGAGGAAGACTGGGAGGGGTGCCTGTCCGTGCCGGGGCTGCGGGGCGTGGTGCCGC
GCTGGTCACGCATCCACTACAGGGGCTTCGACGAGCACGGCGCGCCGATCGACCGCACGGTGGAAGGCTTCCATGCCCGG
GTGGTGCAGCACGAGTGCGACCACCTAGTGGGCAAGCTGTACCCGATGCGCATCCGCGACTTCTCGCGCTTCGGCTATGC
GGACGTGCTGTTCCCGGACATCGCGGAGCACGAGGACGACTGA

Upstream 100 bases:

>100_bases
CACCGGCGCCGGCAGCGGCGCATGCGCGGGAGGCGGGGGCAGGGGATCCGTCGGCGGTGCGGGCATGGGGCGATTGTGCC
TGCCGATGGGCAGGGGCGAT

Downstream 100 bases:

>100_bases
CGCGCTGGGTCGCCTCCCCGGCTGCCGGGCGGTGGCTCAGCCCGACAGCGCTTCCAGCAGTTCGGTCTCGATCTGGATCT
GCCGCGCGTTGTGCTGCAGT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MLRVPPDRRRSAPPPTSRAFYMAIHDILKMGDERLLRTARPVAEFGTPSLLQLVQDMRDTMRAVNGAGLAAPQIGVDLQV
VIFGSEERNPRYPDRPLVPPTVLVNPRITPLGGEEEEDWEGCLSVPGLRGVVPRWSRIHYRGFDEHGAPIDRTVEGFHAR
VVQHECDHLVGKLYPMRIRDFSRFGYADVLFPDIAEHEDD

Sequences:

>Translated_200_residues
MLRVPPDRRRSAPPPTSRAFYMAIHDILKMGDERLLRTARPVAEFGTPSLLQLVQDMRDTMRAVNGAGLAAPQIGVDLQV
VIFGSEERNPRYPDRPLVPPTVLVNPRITPLGGEEEEDWEGCLSVPGLRGVVPRWSRIHYRGFDEHGAPIDRTVEGFHAR
VVQHECDHLVGKLYPMRIRDFSRFGYADVLFPDIAEHEDD
>Mature_200_residues
MLRVPPDRRRSAPPPTSRAFYMAIHDILKMGDERLLRTARPVAEFGTPSLLQLVQDMRDTMRAVNGAGLAAPQIGVDLQV
VIFGSEERNPRYPDRPLVPPTVLVNPRITPLGGEEEEDWEGCLSVPGLRGVVPRWSRIHYRGFDEHGAPIDRTVEGFHAR
VVQHECDHLVGKLYPMRIRDFSRFGYADVLFPDIAEHEDD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=177, Percent_Identity=34.4632768361582, Blast_Score=78, Evalue=5e-15,
Organism=Escherichia coli, GI1789682, Length=154, Percent_Identity=37.6623376623377, Blast_Score=101, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24645728, Length=167, Percent_Identity=30.5389221556886, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=32.0512820512821, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22641; Mature: 22641

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVPPDRRRSAPPPTSRAFYMAIHDILKMGDERLLRTARPVAEFGTPSLLQLVQDMRDT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MRAVNGAGLAAPQIGVDLQVVIFGSEERNPRYPDRPLVPPTVLVNPRITPLGGEEEEDWE
HHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCEECCCCCCCCHHHH
GCLSVPGLRGVVPRWSRIHYRGFDEHGAPIDRTVEGFHARVVQHECDHLVGKLYPMRIRD
HHHCCCCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FSRFGYADVLFPDIAEHEDD
HHHCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MLRVPPDRRRSAPPPTSRAFYMAIHDILKMGDERLLRTARPVAEFGTPSLLQLVQDMRDT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MRAVNGAGLAAPQIGVDLQVVIFGSEERNPRYPDRPLVPPTVLVNPRITPLGGEEEEDWE
HHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCEECCCCCCCCHHHH
GCLSVPGLRGVVPRWSRIHYRGFDEHGAPIDRTVEGFHARVVQHECDHLVGKLYPMRIRD
HHHCCCCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FSRFGYADVLFPDIAEHEDD
HHHCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]