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The map label for this gene is thiE [H]

Identifier: 120611733

GI number: 120611733

Start: 3384087

End: 3384995

Strand: Direct

Name: thiE [H]

Synonym: Aave_3071

Alternate gene names: 120611733

Gene position: 3384087-3384995 (Clockwise)

Preceding gene: 120611732

Following gene: 120611735

Centisome position: 63.22

GC content: 74.15

Gene sequence:

>909_bases
ATGGCTGAAGATTCCACCCTCCAGGCGATGGCCGAGGCCATCGTGCAGGCCCACGCGGCGGCCTTCGCCGGCTTTCCCGC
CCAGCCCGCGCCGGCCCCTGCGCACGGTGGAGCGGCCTATGGCGCCGCCCTGCGCGCCTGCAGCCAGCTCGGCTTCATCG
CCGTGGACGCCGACTGCCTCGCGCGCGCCTGGCAGGCCCGGACGGACCGCACGGGCCATTTCGACGCATCGCATTGGCCC
GACGAGCCCGTGGACTTCGGCCTGCAGCCCCGGCCCCACGCCCGCCCCTTCGCGACCTGCCCGGACAGGCTCGGCCTGTA
CGCCGTGCTGCCCGATGCCCACTGGGTGGGCCGCATGGCCCGGGCCGGCGTGCCGACGGTGCAGCTGCGCTTCAAGTCGG
AAGACGGCGCGGCCGTCGAGCGCGAAGTGCGGGCCGCAGTAGAGGCGGTGCGGGGCACCTCCGCCCTGCTCTTCATCAAC
GACCACTGGCAGGCCGCCATCGGGGCCGGTGCCTATGGCGTCCACCTGGGGCAGGAAGACCTCGATGCCCTGCCCCCCGG
CTCGCTGGAGACGCTGCGCGCCTCCGGATTGCGGCTCGGGGTGAGCACGCACGGGTACGCCGAGATGGTGCGCGCCGACG
CGGCGGCTCCCAGCTACGTGGCCATGGGCGCGGTCTTCCCCACCACGCTCAAGAAGATGGCGACCGTGCCCCAGGGGCTG
GCCCGCCTCGCGGGCTATGCGCGGCTCATGCGCGGCTACCCGCAGGTGGCGATCGGCGGGATCGGCGCGGAACAGTTCGC
CGATGTGCTCGCGACCGGCGTCGGGTCCATCGCCGTGGTGCGCGCGCTGGTGAACGCCCCCGATCCGGAAGCCGCGGCGC
GCGACCTCATGCAGCGCATGGGGCGGTAG

Upstream 100 bases:

>100_bases
CAGGCCGGACGCACCGCCCGGCAGGCCGGCGCCATGTCCGCGCAGGACGCCGCCCAGCCGAGCACGCCCGTGCTCGGCAC
CCCTTTCTGGCACCACGACC

Downstream 100 bases:

>100_bases
CCCTCGTCCGCCGGCCGGCGGACCCCGGGCATGCCCGCCGAGGCCCGCGCCGCCGCCGGCACCGCATCAGCCCTTGGGCG
TGCGGTTCTTGCGATCGAAC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MAEDSTLQAMAEAIVQAHAAAFAGFPAQPAPAPAHGGAAYGAALRACSQLGFIAVDADCLARAWQARTDRTGHFDASHWP
DEPVDFGLQPRPHARPFATCPDRLGLYAVLPDAHWVGRMARAGVPTVQLRFKSEDGAAVEREVRAAVEAVRGTSALLFIN
DHWQAAIGAGAYGVHLGQEDLDALPPGSLETLRASGLRLGVSTHGYAEMVRADAAAPSYVAMGAVFPTTLKKMATVPQGL
ARLAGYARLMRGYPQVAIGGIGAEQFADVLATGVGSIAVVRALVNAPDPEAAARDLMQRMGR

Sequences:

>Translated_302_residues
MAEDSTLQAMAEAIVQAHAAAFAGFPAQPAPAPAHGGAAYGAALRACSQLGFIAVDADCLARAWQARTDRTGHFDASHWP
DEPVDFGLQPRPHARPFATCPDRLGLYAVLPDAHWVGRMARAGVPTVQLRFKSEDGAAVEREVRAAVEAVRGTSALLFIN
DHWQAAIGAGAYGVHLGQEDLDALPPGSLETLRASGLRLGVSTHGYAEMVRADAAAPSYVAMGAVFPTTLKKMATVPQGL
ARLAGYARLMRGYPQVAIGGIGAEQFADVLATGVGSIAVVRALVNAPDPEAAARDLMQRMGR
>Mature_301_residues
AEDSTLQAMAEAIVQAHAAAFAGFPAQPAPAPAHGGAAYGAALRACSQLGFIAVDADCLARAWQARTDRTGHFDASHWPD
EPVDFGLQPRPHARPFATCPDRLGLYAVLPDAHWVGRMARAGVPTVQLRFKSEDGAAVEREVRAAVEAVRGTSALLFIND
HWQAAIGAGAYGVHLGQEDLDALPPGSLETLRASGLRLGVSTHGYAEMVRADAAAPSYVAMGAVFPTTLKKMATVPQGLA
RLAGYARLMRGYPQVAIGGIGAEQFADVLATGVGSIAVVRALVNAPDPEAAARDLMQRMGR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=205, Percent_Identity=49.7560975609756, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 31595; Mature: 31464

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDSTLQAMAEAIVQAHAAAFAGFPAQPAPAPAHGGAAYGAALRACSQLGFIAVDADCL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECHHHH
ARAWQARTDRTGHFDASHWPDEPVDFGLQPRPHARPFATCPDRLGLYAVLPDAHWVGRMA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHH
RAGVPTVQLRFKSEDGAAVEREVRAAVEAVRGTSALLFINDHWQAAIGAGAYGVHLGQED
HCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCCEEECCHHH
LDALPPGSLETLRASGLRLGVSTHGYAEMVRADAAAPSYVAMGAVFPTTLKKMATVPQGL
HHCCCCCCHHHHHHCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARLAGYARLMRGYPQVAIGGIGAEQFADVLATGVGSIAVVRALVNAPDPEAAARDLMQRM
HHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
GR
CC
>Mature Secondary Structure 
AEDSTLQAMAEAIVQAHAAAFAGFPAQPAPAPAHGGAAYGAALRACSQLGFIAVDADCL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECHHHH
ARAWQARTDRTGHFDASHWPDEPVDFGLQPRPHARPFATCPDRLGLYAVLPDAHWVGRMA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHH
RAGVPTVQLRFKSEDGAAVEREVRAAVEAVRGTSALLFINDHWQAAIGAGAYGVHLGQED
HCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCCEEECCHHH
LDALPPGSLETLRASGLRLGVSTHGYAEMVRADAAAPSYVAMGAVFPTTLKKMATVPQGL
HHCCCCCCHHHHHHCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARLAGYARLMRGYPQVAIGGIGAEQFADVLATGVGSIAVVRALVNAPDPEAAARDLMQRM
HHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
GR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA