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The map label for this gene is thiG

Identifier: 120611732

GI number: 120611732

Start: 3383285

End: 3384094

Strand: Direct

Name: thiG

Synonym: Aave_3070

Alternate gene names: 120611732

Gene position: 3383285-3384094 (Clockwise)

Preceding gene: 120611731

Following gene: 120611733

Centisome position: 63.21

GC content: 72.96

Gene sequence:

>810_bases
ATGCCCGACACCGCATCCGATGACGCCCTGGTCCTCTACGGCCAGCGCTTCGCCAGCCGGCTCCTGCTGGGAACCGCCCG
CTATCCCTCGCCCAGCGTGCTGGAGGCCGCCGTGCGGCGCGCCCGCCCGGCCATGGTGACCGCCTCGCTGCGGCGCCAGG
GCAGCAACCCCGCGGAAGCCGGCAGCGGCTTCTGGGAACTGCTGCGCCGGCTGGACGTGCCGGTGCTGCCGAACACCGCC
GGCTGCCACAGCATCCAGGAAGCCGTCACCACCGCCCAGATGGCCCGCGAGGTCTTCGGCACGCCCTGGATCAAGCTGGA
GCTGATCGGCGACGACTACACGCTGCAGCCCGACACGCTGAACCTCGTGGAGGCCGCCTCCCAGCTGGTACGCGACGGCT
TCCACGTGCTGCCCTACTGCACCGAAGACCTCGTGCTCTGCCAGCGCCTGGTGGACGTGGGCTGCCAGGCCGTCATGCCC
TGGGCCGCGCCCATCGGCACCGGCCGTGGCCCCGTCAATCCGTACGCGCTGCGCACGCTGCGCGAGCGGCTGGACGTACC
CCTGCTCGTGGATGCGGGCCTGGGCCTGCCCTCGCATGCCTGCCAGGTCATGGAATGGGGCTACGACGGGGTGCTGCTCA
ACACGGCCGTGGCCCTGGCGCAGGACCCGGTGGCCATGGCCGGCGCCTTCGCCGACGCGGTGCAGGCCGGACGCACCGCC
CGGCAGGCCGGCGCCATGTCCGCGCAGGACGCCGCCCAGCCGAGCACGCCCGTGCTCGGCACCCCTTTCTGGCACCACGA
CCATGGCTGA

Upstream 100 bases:

>100_bases
GACCGGGTCGAGATCATCGCGCCGGTGACGGGCGGCTGACGCCCCTGCCCGGCAGCCTGCCGACGCAGGCCGCCCAGCCC
TTCCCTTTCCGACATCCGCC

Downstream 100 bases:

>100_bases
AGATTCCACCCTCCAGGCGATGGCCGAGGCCATCGTGCAGGCCCACGCGGCGGCCTTCGCCGGCTTTCCCGCCCAGCCCG
CGCCGGCCCCTGCGCACGGT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPDTASDDALVLYGQRFASRLLLGTARYPSPSVLEAAVRRARPAMVTASLRRQGSNPAEAGSGFWELLRRLDVPVLPNTA
GCHSIQEAVTTAQMAREVFGTPWIKLELIGDDYTLQPDTLNLVEAASQLVRDGFHVLPYCTEDLVLCQRLVDVGCQAVMP
WAAPIGTGRGPVNPYALRTLRERLDVPLLVDAGLGLPSHACQVMEWGYDGVLLNTAVALAQDPVAMAGAFADAVQAGRTA
RQAGAMSAQDAAQPSTPVLGTPFWHHDHG

Sequences:

>Translated_269_residues
MPDTASDDALVLYGQRFASRLLLGTARYPSPSVLEAAVRRARPAMVTASLRRQGSNPAEAGSGFWELLRRLDVPVLPNTA
GCHSIQEAVTTAQMAREVFGTPWIKLELIGDDYTLQPDTLNLVEAASQLVRDGFHVLPYCTEDLVLCQRLVDVGCQAVMP
WAAPIGTGRGPVNPYALRTLRERLDVPLLVDAGLGLPSHACQVMEWGYDGVLLNTAVALAQDPVAMAGAFADAVQAGRTA
RQAGAMSAQDAAQPSTPVLGTPFWHHDHG
>Mature_268_residues
PDTASDDALVLYGQRFASRLLLGTARYPSPSVLEAAVRRARPAMVTASLRRQGSNPAEAGSGFWELLRRLDVPVLPNTAG
CHSIQEAVTTAQMAREVFGTPWIKLELIGDDYTLQPDTLNLVEAASQLVRDGFHVLPYCTEDLVLCQRLVDVGCQAVMPW
AAPIGTGRGPVNPYALRTLRERLDVPLLVDAGLGLPSHACQVMEWGYDGVLLNTAVALAQDPVAMAGAFADAVQAGRTAR
QAGAMSAQDAAQPSTPVLGTPFWHHDHG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=246, Percent_Identity=43.0894308943089, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_ACIAC (A1TRP8)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971410.1
- ProteinModelPortal:   A1TRP8
- SMR:   A1TRP8
- STRING:   A1TRP8
- GeneID:   4667433
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3070
- NMPDR:   fig|397945.5.peg.2613
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   A1TRP8
- ProtClustDB:   PRK00208
- BioCyc:   AAVE397945:AAVE_3070-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28790; Mature: 28659

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: ACT_SITE 105-105 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTASDDALVLYGQRFASRLLLGTARYPSPSVLEAAVRRARPAMVTASLRRQGSNPAEA
CCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHC
GSGFWELLRRLDVPVLPNTAGCHSIQEAVTTAQMAREVFGTPWIKLELIGDDYTLQPDTL
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCHH
NLVEAASQLVRDGFHVLPYCTEDLVLCQRLVDVGCQAVMPWAAPIGTGRGPVNPYALRTL
HHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCHHHHHHH
RERLDVPLLVDAGLGLPSHACQVMEWGYDGVLLNTAVALAQDPVAMAGAFADAVQAGRTA
HHHCCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RQAGAMSAQDAAQPSTPVLGTPFWHHDHG
HHHCCCCHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDTASDDALVLYGQRFASRLLLGTARYPSPSVLEAAVRRARPAMVTASLRRQGSNPAEA
CCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHC
GSGFWELLRRLDVPVLPNTAGCHSIQEAVTTAQMAREVFGTPWIKLELIGDDYTLQPDTL
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCHH
NLVEAASQLVRDGFHVLPYCTEDLVLCQRLVDVGCQAVMPWAAPIGTGRGPVNPYALRTL
HHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCHHHHHHH
RERLDVPLLVDAGLGLPSHACQVMEWGYDGVLLNTAVALAQDPVAMAGAFADAVQAGRTA
HHHCCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RQAGAMSAQDAAQPSTPVLGTPFWHHDHG
HHHCCCCHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA