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The map label for this gene is mnmC

Identifier: 120611711

GI number: 120611711

Start: 3357410

End: 3359317

Strand: Direct

Name: mnmC

Synonym: Aave_3048

Alternate gene names: 120611711

Gene position: 3357410-3359317 (Clockwise)

Preceding gene: 120611710

Following gene: 120611714

Centisome position: 62.72

GC content: 75.63

Gene sequence:

>1908_bases
ATGTCCGAACCCATCGACTGGCTCCCCGACGGCACCCCGTTCAATCCGCGCTTCGGCGACCGCTACCACAGCCACACCGG
GCTCGCGCAGGCACGCGAAGTCTTCCTGCGCGGCTGCGGCCTGCCCGGGGCGTGGGCCGGCCAGGCGATGTGGCGGGTGC
TGGAGACCGGCTTCGGCTGCGGGCTGAATTTCCTCGCCACCTGGGCCGCCTGGCGGGCCGACCCGGCGCGGCCCCGGCTG
CTGCATTTCGTGTCCTGCGAAGCGTTTCCCGTCTCAGCGGACGACCTGCTGCGCTCCATGCAGGGGCATGGCGAGCTGGA
ACCCCTGGCCCGCGCGCTGCATGCGCAGTACTGGGGCCTGCTGCCCGGCGTGCACCGGCTCGCCTTCGAGGGCGGGCAGG
TGCTGCTCACGCTCTACATCGGCGACGCGCAGGCGATGCTGCGCCAGCAGCAGCCGGTGGCCGATGCGGTGTACCTGGAC
GGCTTCAGCCCGCAGGTGAACCCGGAGCTGTGGGACGTGCACACGCTCAAGGCCGTGGCGCGCTGCTGCCGCCGCGGCAC
GCGCCTGGCCACCTGGAGCGTGGCCGGCGCGGTGCGCGAAGGGCTCGCGCAGTGCGGTTTCCGGGTGCAGAAGGTGCCCG
GCCTGCCCCCGAAGCGCAGCAACCTGCAGGCGGAGTTCGATCCGGCCTGGGAGCCGCGCCCCGTGCAGCCGGAGCTGCCC
GACGTGCGCGTGGCGGGCGGACCGTCGAGCGTGCTGGTCATCGGCGGAGGGCTGTCGGGCGCCGCCGTGGCGGCGAGCCT
CGCGCGGCGCGGCTGGCAGGTGCGGGTGCTGGACCAGGGCGCGGAACCCGCGGCCGGCGCCTCGGGCCTGCCGGCCGGCG
TGTTCGCGCCGCATGTGTCGCCCGACGACAGCCTGCTCTCGCGCCTGTCGCGCTGCGGCGTGCGGGCCACGCTGCAGGCC
CTGGAGGCATCCGGCCTGTCGGAAGGCGAGGACTGGAGCGCCTGCGGCGTGCTGGAGCACGAGGTGGATGGCAAGCACCG
CCTGCCGCCTGCCTGGGCCGGGGATGCCGCGGTGGGCGCCGAATGGAGCCGCCCCGCCGATGCCGCGGCGCTGGAGGCCG
CTGGCCTGCCCGGAGCCACCGTCGCCTATTGGCATGCGCGTGGCGGATGGCTGCGGCCGGCCCGGCTGGTGCGCGCGCTG
CTGGCGCACCCTGGCATCCACTGGCAGGGCCGCAGTGCCGTGGCACGGCTGGAGCCCGTGGCCGCTCCCGGTGGCGCCAC
GCACTGGCAGGCATATGGCAGCGACGGCACGGTGCTCGCCGAAGCGCCCCACGTGGTGGTGGCCGCTGGCTACGGCAGCC
GCCCCTGGCTACCCGCCCGCTACCCGATCCATCCGCTGCGCGGCCAGGTGAGCTGGGGCACGCGGGCGGACAGCCCGGCC
GCCGCCTGGCCGCCCTTCCCCGTCAACGGCCATGGCAACCTCGTGCCGCATGCGGGCACGGCGGGGGGAGGCATCTGGGT
GATGGGCTCGACCTTCGAGCGCGGCCAGACGGAACTGCCCCCCGCGCCCCAGGAACAGGCGCAGGCCCATGCGGCCAATG
CCGCGAAGCTGGAACAACTGCTGCCGGCGCTGGCGCAGGGGCTCGCACCCGCCATCGCCGGCCCGGGCGCCGCCCCCGGC
CACTGGGCGGCCGTGCGCTGCACCGCGCCCGACCGGCTGCCTTTCGTGGGGCCGGTGGATGCCGCACGGCAGGCCGGGCT
CTGGGTCTGCGCCGCGATGGGCGCGCGCGGGCTCACGCTGTCGCAGCTGTGCGGGGAACTGCTGGCCGCCCGCATGATGG
GCGAGCCGCTGCCTGTGGAGGTGCGTCTGGCGCGGGCGCTGTCCACCGAGCGGCTTCCTGCGGACTGA

Upstream 100 bases:

>100_bases
GCCCGGCCCCGCCGGGCTTTCTTTTTTTCCGCCCCCGTCCGCGCTCTGCGACTGACACGGCGCAACCTTGCGCACCCCGC
GGCGCCCCGACAATCGCCGC

Downstream 100 bases:

>100_bases
GACCTCATGGCCCGGCCCGGGCGGAGCGCTCAGCCCGGCGGTGCCTTCGCCTTCCGTGCCGGAATCCACACGCCGAGCAT
GGCCGCCGCCGCGAGCGAGA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MSEPIDWLPDGTPFNPRFGDRYHSHTGLAQAREVFLRGCGLPGAWAGQAMWRVLETGFGCGLNFLATWAAWRADPARPRL
LHFVSCEAFPVSADDLLRSMQGHGELEPLARALHAQYWGLLPGVHRLAFEGGQVLLTLYIGDAQAMLRQQQPVADAVYLD
GFSPQVNPELWDVHTLKAVARCCRRGTRLATWSVAGAVREGLAQCGFRVQKVPGLPPKRSNLQAEFDPAWEPRPVQPELP
DVRVAGGPSSVLVIGGGLSGAAVAASLARRGWQVRVLDQGAEPAAGASGLPAGVFAPHVSPDDSLLSRLSRCGVRATLQA
LEASGLSEGEDWSACGVLEHEVDGKHRLPPAWAGDAAVGAEWSRPADAAALEAAGLPGATVAYWHARGGWLRPARLVRAL
LAHPGIHWQGRSAVARLEPVAAPGGATHWQAYGSDGTVLAEAPHVVVAAGYGSRPWLPARYPIHPLRGQVSWGTRADSPA
AAWPPFPVNGHGNLVPHAGTAGGGIWVMGSTFERGQTELPPAPQEQAQAHAANAAKLEQLLPALAQGLAPAIAGPGAAPG
HWAAVRCTAPDRLPFVGPVDAARQAGLWVCAAMGARGLTLSQLCGELLAARMMGEPLPVEVRLARALSTERLPAD

Sequences:

>Translated_635_residues
MSEPIDWLPDGTPFNPRFGDRYHSHTGLAQAREVFLRGCGLPGAWAGQAMWRVLETGFGCGLNFLATWAAWRADPARPRL
LHFVSCEAFPVSADDLLRSMQGHGELEPLARALHAQYWGLLPGVHRLAFEGGQVLLTLYIGDAQAMLRQQQPVADAVYLD
GFSPQVNPELWDVHTLKAVARCCRRGTRLATWSVAGAVREGLAQCGFRVQKVPGLPPKRSNLQAEFDPAWEPRPVQPELP
DVRVAGGPSSVLVIGGGLSGAAVAASLARRGWQVRVLDQGAEPAAGASGLPAGVFAPHVSPDDSLLSRLSRCGVRATLQA
LEASGLSEGEDWSACGVLEHEVDGKHRLPPAWAGDAAVGAEWSRPADAAALEAAGLPGATVAYWHARGGWLRPARLVRAL
LAHPGIHWQGRSAVARLEPVAAPGGATHWQAYGSDGTVLAEAPHVVVAAGYGSRPWLPARYPIHPLRGQVSWGTRADSPA
AAWPPFPVNGHGNLVPHAGTAGGGIWVMGSTFERGQTELPPAPQEQAQAHAANAAKLEQLLPALAQGLAPAIAGPGAAPG
HWAAVRCTAPDRLPFVGPVDAARQAGLWVCAAMGARGLTLSQLCGELLAARMMGEPLPVEVRLARALSTERLPAD
>Mature_634_residues
SEPIDWLPDGTPFNPRFGDRYHSHTGLAQAREVFLRGCGLPGAWAGQAMWRVLETGFGCGLNFLATWAAWRADPARPRLL
HFVSCEAFPVSADDLLRSMQGHGELEPLARALHAQYWGLLPGVHRLAFEGGQVLLTLYIGDAQAMLRQQQPVADAVYLDG
FSPQVNPELWDVHTLKAVARCCRRGTRLATWSVAGAVREGLAQCGFRVQKVPGLPPKRSNLQAEFDPAWEPRPVQPELPD
VRVAGGPSSVLVIGGGLSGAAVAASLARRGWQVRVLDQGAEPAAGASGLPAGVFAPHVSPDDSLLSRLSRCGVRATLQAL
EASGLSEGEDWSACGVLEHEVDGKHRLPPAWAGDAAVGAEWSRPADAAALEAAGLPGATVAYWHARGGWLRPARLVRALL
AHPGIHWQGRSAVARLEPVAAPGGATHWQAYGSDGTVLAEAPHVVVAAGYGSRPWLPARYPIHPLRGQVSWGTRADSPAA
AWPPFPVNGHGNLVPHAGTAGGGIWVMGSTFERGQTELPPAPQEQAQAHAANAAKLEQLLPALAQGLAPAIAGPGAAPGH
WAAVRCTAPDRLPFVGPVDAARQAGLWVCAAMGARGLTLSQLCGELLAARMMGEPLPVEVRLARALSTERLPAD

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=664, Percent_Identity=31.3253012048193, Blast_Score=216, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_ACIAC (A1TRM7)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971389.1
- ProteinModelPortal:   A1TRM7
- SMR:   A1TRM7
- STRING:   A1TRM7
- GeneID:   4669010
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3048
- NMPDR:   fig|397945.5.peg.2595
- eggNOG:   COG4121
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   A1TRM7
- ProtClustDB:   CLSK951408
- BioCyc:   AAVE397945:AAVE_3048-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 66955; Mature: 66824

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPIDWLPDGTPFNPRFGDRYHSHTGLAQAREVFLRGCGLPGAWAGQAMWRVLETGFGC
CCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
GLNFLATWAAWRADPARPRLLHFVSCEAFPVSADDLLRSMQGHGELEPLARALHAQYWGL
CHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LPGVHRLAFEGGQVLLTLYIGDAQAMLRQQQPVADAVYLDGFSPQVNPELWDVHTLKAVA
HHHHHHHHHCCCEEEEEEEECCHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
RCCRRGTRLATWSVAGAVREGLAQCGFRVQKVPGLPPKRSNLQAEFDPAWEPRPVQPELP
HHHHCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DVRVAGGPSSVLVIGGGLSGAAVAASLARRGWQVRVLDQGAEPAAGASGLPAGVFAPHVS
CEEEECCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
PDDSLLSRLSRCGVRATLQALEASGLSEGEDWSACGVLEHEVDGKHRLPPAWAGDAAVGA
CCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
EWSRPADAAALEAAGLPGATVAYWHARGGWLRPARLVRALLAHPGIHWQGRSAVARLEPV
CCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCC
AAPGGATHWQAYGSDGTVLAEAPHVVVAAGYGSRPWLPARYPIHPLRGQVSWGTRADSPA
CCCCCCCCEEEECCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAWPPFPVNGHGNLVPHAGTAGGGIWVMGSTFERGQTELPPAPQEQAQAHAANAAKLEQL
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LPALAQGLAPAIAGPGAAPGHWAAVRCTAPDRLPFVGPVDAARQAGLWVCAAMGARGLTL
HHHHHHHCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCH
SQLCGELLAARMMGEPLPVEVRLARALSTERLPAD
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEPIDWLPDGTPFNPRFGDRYHSHTGLAQAREVFLRGCGLPGAWAGQAMWRVLETGFGC
CCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
GLNFLATWAAWRADPARPRLLHFVSCEAFPVSADDLLRSMQGHGELEPLARALHAQYWGL
CHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LPGVHRLAFEGGQVLLTLYIGDAQAMLRQQQPVADAVYLDGFSPQVNPELWDVHTLKAVA
HHHHHHHHHCCCEEEEEEEECCHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
RCCRRGTRLATWSVAGAVREGLAQCGFRVQKVPGLPPKRSNLQAEFDPAWEPRPVQPELP
HHHHCCCEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DVRVAGGPSSVLVIGGGLSGAAVAASLARRGWQVRVLDQGAEPAAGASGLPAGVFAPHVS
CEEEECCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
PDDSLLSRLSRCGVRATLQALEASGLSEGEDWSACGVLEHEVDGKHRLPPAWAGDAAVGA
CCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
EWSRPADAAALEAAGLPGATVAYWHARGGWLRPARLVRALLAHPGIHWQGRSAVARLEPV
CCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCC
AAPGGATHWQAYGSDGTVLAEAPHVVVAAGYGSRPWLPARYPIHPLRGQVSWGTRADSPA
CCCCCCCCEEEECCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAWPPFPVNGHGNLVPHAGTAGGGIWVMGSTFERGQTELPPAPQEQAQAHAANAAKLEQL
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LPALAQGLAPAIAGPGAAPGHWAAVRCTAPDRLPFVGPVDAARQAGLWVCAAMGARGLTL
HHHHHHHCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCH
SQLCGELLAARMMGEPLPVEVRLARALSTERLPAD
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA