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The map label for this gene is gloA [H]

Identifier: 120611455

GI number: 120611455

Start: 3064274

End: 3064690

Strand: Direct

Name: gloA [H]

Synonym: Aave_2791

Alternate gene names: 120611455

Gene position: 3064274-3064690 (Clockwise)

Preceding gene: 120611454

Following gene: 120611462

Centisome position: 57.25

GC content: 65.95

Gene sequence:

>417_bases
ATGCGATTCCTCCACACGATGCTGCGCGTTGGCAATCTCCAGCGCTCGATTGATTTCTACACCCAGGTGCTCGGCATGCA
ACTGCTGCGCACGTCCGAGAACCCCGAGTACAAGTACTCGCTGGCGTTCCTGGGCTTCGACGGCGGCAACCCGGGCCAGG
CCGAGATCGAACTCACCTACAACTGGGGCACCGAGAGCTACGACATGGGCACGGCCTACGGCCACATCGCCCTGGGCGTG
CCGGACGCCTACGCGGCCTGCGAGAAGATCAAGGCCGCTGGCGGCAACGTCACGCGCGAGGCCGGCCCGGTCAAGGGCGG
CACGACGGTGATCGCCTTCGTGACGGACCCGGACGGCTACAAGATCGAGCTGATCGAACGCAAGAACGACGTGGGCGCGG
GCACCGGCCTGCGCTGA

Upstream 100 bases:

>100_bases
CGAGATCGTCACGGTGGACGGCGGGCGCATGACGCTCAACTACACCGTGCCCTTGGGTGGCTGAGCGCCGGGTTTTCGAT
CTCGCGGCACAATCGGCGGC

Downstream 100 bases:

>100_bases
CGCGGACTTGCGCGGCCGGCCGCGCGTGTCACGGTGCGTATCCCGCGTCCACCGCCGGAGGCCGCAGCAGGTCCGCATTG
ACCCCCATCGCCTCGCTGGC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MRFLHTMLRVGNLQRSIDFYTQVLGMQLLRTSENPEYKYSLAFLGFDGGNPGQAEIELTYNWGTESYDMGTAYGHIALGV
PDAYAACEKIKAAGGNVTREAGPVKGGTTVIAFVTDPDGYKIELIERKNDVGAGTGLR

Sequences:

>Translated_138_residues
MRFLHTMLRVGNLQRSIDFYTQVLGMQLLRTSENPEYKYSLAFLGFDGGNPGQAEIELTYNWGTESYDMGTAYGHIALGV
PDAYAACEKIKAAGGNVTREAGPVKGGTTVIAFVTDPDGYKIELIERKNDVGAGTGLR
>Mature_138_residues
MRFLHTMLRVGNLQRSIDFYTQVLGMQLLRTSENPEYKYSLAFLGFDGGNPGQAEIELTYNWGTESYDMGTAYGHIALGV
PDAYAACEKIKAAGGNVTREAGPVKGGTTVIAFVTDPDGYKIELIERKNDVGAGTGLR

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=144, Percent_Identity=38.1944444444444, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1787940, Length=136, Percent_Identity=76.4705882352941, Blast_Score=207, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=41.0071942446043, Blast_Score=101, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=39.7260273972603, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15001; Mature: 15001

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLHTMLRVGNLQRSIDFYTQVLGMQLLRTSENPEYKYSLAFLGFDGGNPGQAEIELTY
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCEEEEEEE
NWGTESYDMGTAYGHIALGVPDAYAACEKIKAAGGNVTREAGPVKGGTTVIAFVTDPDGY
CCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCC
KIELIERKNDVGAGTGLR
EEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRFLHTMLRVGNLQRSIDFYTQVLGMQLLRTSENPEYKYSLAFLGFDGGNPGQAEIELTY
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCEEEEEEE
NWGTESYDMGTAYGHIALGVPDAYAACEKIKAAGGNVTREAGPVKGGTTVIAFVTDPDGY
CCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCC
KIELIERKNDVGAGTGLR
EEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]