Click here to switch to the map view.

The map label for this gene is dmlA [H]

Identifier: 120610765

GI number: 120610765

Start: 2290856

End: 2291953

Strand: Reverse

Name: dmlA [H]

Synonym: Aave_2087

Alternate gene names: 120610765

Gene position: 2291953-2290856 (Counterclockwise)

Preceding gene: 120610769

Following gene: 120610764

Centisome position: 42.82

GC content: 69.31

Gene sequence:

>1098_bases
ATGACCACCCCCTCCCGCACCTTCAAGATCGCCGTCCTGCCCGGCGACGGCATCGGCAAGGAAGTCATGCCCGAGGGGCT
GCGGGCGATCCAGGCGGCGGCCGAGCGTTTCGGCTTCGCGCTGGAGCTGCACACCTTCGAGTGGGCCAGCTGCGACTACT
ACCTCGCGCACGGGCAGATGATGCCCGACGACTGGAAGGAGCAGCTCAAGGGCATGGACGCGCTCTTCTTCGGCGCCGTG
GGCTGGCCGGCCACCGTGCCCGACCACGTCTCGCTGTGGGGCTCGCTGCTGAAGTTCCGCCGCGAGTTCGACCAGTACAT
CAACCTGCGGCCCGTGCGCCTGTTCGAGGGCGTGCCGTGCCCGCTGGCGGGCCGCAAGCCCGGGGACATCGACTACTACG
TGGTGCGCGAGAACACCGAGGGCGAGTACACGTCGCTGGGCGGCATCATGTACGAGGGCACGGACCGCGAGATCGTGATC
CAGGAGTCGGTGTATTCGCGCAAGGGCGCCGAGCGGCTGCTGAAGTTCGCGTTCGATCTCGCGCAGAGCCGCCCGAAGAA
GCACGTGACGCTGGCCACCAAGAGCAACGGCATCGCCATCAGCATGCCGTGGTGGGACCGGCGGGCCGACGAGGTGGCCA
GACAGTATCCCGGCATCACGCTGGACAAGCAGCACATCGACATCCTGACGGCGCGCTTCGTGCTGCAGCCGGGGCGCTTC
GACGTGGTGGCGGCGACGAACCTGTTCGGCGACATCCTGTCGGACCTGGGGCCGGCGACGACGGGCACGATCGGGCTGGC
GCCGTCGGCGAACCTGAACCCGGAGCGCACGTTCCCGAGCCTGTTCGAGCCGGTGCACGGCTCGGCGCCGGACATCTACG
GCAGGAACATCGCCAACCCGATCGCGATGGTGTGGTCGGGGGCGCTGATGCTGGACTTCCTGACGCAGGGCCAGGGGGCG
GGGCGGCAGGCGCACGATGCGATCGTGGCGGCCATCGAGCAGGTGATCCAGAGCGGGCCGAAGACGCCGGACCTGGGCGG
CACGGCCAGCACCACGCAGGTGGGCGAAGCCATCGCGGCGGCCATCGCCCGGAGCTGA

Upstream 100 bases:

>100_bases
TGCGGTTTGCCGAAGAATCGGCGGCATGCGGGCGGCCAGCGCTTTGGCGTGCCGGCCTTCCCGTCCATCCTTTCCACGCT
TTTTTCCGGAGACTTCGCCC

Downstream 100 bases:

>100_bases
CCAGTGGGGCCGAGCGCCGGCCACGCGACCCCACGCGCGCAAGGCGGGCGGCAGCCCGATAAACTGGCCGCTTCCCCACA
GTGTCTCCCTGCCGGCATGC

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTTPSRTFKIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQMMPDDWKEQLKGMDALFFGAV
GWPATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPCPLAGRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVI
QESVYSRKGAERLLKFAFDLAQSRPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRF
DVVAATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANPIAMVWSGALMLDFLTQGQGA
GRQAHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAAAIARS

Sequences:

>Translated_365_residues
MTTPSRTFKIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQMMPDDWKEQLKGMDALFFGAV
GWPATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPCPLAGRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVI
QESVYSRKGAERLLKFAFDLAQSRPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRF
DVVAATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANPIAMVWSGALMLDFLTQGQGA
GRQAHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAAAIARS
>Mature_364_residues
TTPSRTFKIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQMMPDDWKEQLKGMDALFFGAVG
WPATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPCPLAGRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVIQ
ESVYSRKGAERLLKFAFDLAQSRPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRFD
VVAATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANPIAMVWSGALMLDFLTQGQGAG
RQAHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAAAIARS

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=357, Percent_Identity=31.0924369747899, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI28178816, Length=365, Percent_Identity=27.6712328767123, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI28178821, Length=355, Percent_Identity=27.6056338028169, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4758582, Length=351, Percent_Identity=27.0655270655271, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178838, Length=343, Percent_Identity=26.8221574344023, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI28178819, Length=201, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=72.1448467966574, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI87081683, Length=370, Percent_Identity=37.8378378378378, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787381, Length=386, Percent_Identity=28.2383419689119, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17550882, Length=359, Percent_Identity=28.4122562674095, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71986051, Length=359, Percent_Identity=30.6406685236769, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=26.2569832402235, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17505779, Length=355, Percent_Identity=27.0422535211268, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322097, Length=363, Percent_Identity=35.8126721763085, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6319830, Length=367, Percent_Identity=32.6975476839237, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=360, Percent_Identity=31.9444444444444, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=356, Percent_Identity=31.4606741573034, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643270, Length=364, Percent_Identity=32.1428571428571, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24643268, Length=364, Percent_Identity=32.1428571428571, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24661184, Length=355, Percent_Identity=29.0140845070423, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI161078635, Length=365, Percent_Identity=27.9452054794521, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078637, Length=365, Percent_Identity=27.9452054794521, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI161078633, Length=365, Percent_Identity=27.9452054794521, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24650122, Length=365, Percent_Identity=27.9452054794521, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI161078639, Length=363, Percent_Identity=28.099173553719, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI281362242, Length=357, Percent_Identity=28.2913165266106, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648872, Length=357, Percent_Identity=28.2913165266106, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI20130355, Length=362, Percent_Identity=22.9281767955801, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 39912; Mature: 39781

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSRTFKIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQM
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCEEEECCCC
MPDDWKEQLKGMDALFFGAVGWPATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPC
CCHHHHHHHCCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCCC
PLAGRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVIQESVYSRKGAERLLKFAFDL
CCCCCCCCCCEEEEEEECCCCCEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHH
AQSRPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRF
HHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHEECCCCE
DVVAATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANP
EEEEHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCH
IAMVWSGALMLDFLTQGQGAGRQAHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
AIARS
HHHCC
>Mature Secondary Structure 
TTPSRTFKIAVLPGDGIGKEVMPEGLRAIQAAAERFGFALELHTFEWASCDYYLAHGQM
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCEEEECCCC
MPDDWKEQLKGMDALFFGAVGWPATVPDHVSLWGSLLKFRREFDQYINLRPVRLFEGVPC
CCHHHHHHHCCCCEEEHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCCC
PLAGRKPGDIDYYVVRENTEGEYTSLGGIMYEGTDREIVIQESVYSRKGAERLLKFAFDL
CCCCCCCCCCEEEEEEECCCCCEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHH
AQSRPKKHVTLATKSNGIAISMPWWDRRADEVARQYPGITLDKQHIDILTARFVLQPGRF
HHCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHEECCCCE
DVVAATNLFGDILSDLGPATTGTIGLAPSANLNPERTFPSLFEPVHGSAPDIYGRNIANP
EEEEHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCH
IAMVWSGALMLDFLTQGQGAGRQAHDAIVAAIEQVIQSGPKTPDLGGTASTTQVGEAIAA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
AIARS
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]