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The map label for this gene is yegE [H]

Identifier: 120610575

GI number: 120610575

Start: 2050967

End: 2052514

Strand: Direct

Name: yegE [H]

Synonym: Aave_1895

Alternate gene names: 120610575

Gene position: 2050967-2052514 (Clockwise)

Preceding gene: 120610574

Following gene: 120610580

Centisome position: 38.32

GC content: 66.34

Gene sequence:

>1548_bases
ATGCCACGGCGTGGGGCTTGCCGGACGCGGGTGCGTCCGATAATTCATCTCCCCAGGGCCCATACGCACGGAGTTCTCCG
AAAAATGACCTTTCCTCCCTCGTCGGAGAACGATTTTGAACACATGTTCGACCTGGCCCCGGTGTCGCTGTGGCTGGAGG
ACTTCAGCGCGTTGAAGTCGCTGCTCGACGCCTGGCGCAGCGAGGGAGTGGTGGATGCCGAGGCCTACGTGCGGGAGGAC
CCCGCGCGGATGCAGCAGTACGGCGCGAGCCTGAAGGTGCTCCAGGTGAACCGGCGCACGCTGGAGCTGTTCGCGGCGCC
CGACCAGGCCACGCTGCTGGCTTCGCTGGACCAGGTGTTCCGCGGCGACATGTTCGAGCAGGCGATCTCCGAGCTGCGGC
AACTGTGGTCCGGTTCGCTGGAGTTCTCCAACCAGACGGTCAATTACGCGCTCGACGGCCGCCGTCTCGATGTGCACATC
CGCGGCCGCATCCTGCCGGGCCATGAGGACCGCTGGGACCGCGTGCTGATTTCGCTCGAGGACAACACCGAGCAGATCCG
CTCCGGCACGGCATTGCTGCGCAGCGAGCAATACGCGCGCGGCCTGTTCGAGCATTCGCCGGTATCGCTGTGGGTGGAGG
ACTTCAGCTGCGTCAAGCGCCTGCTCGACGACGTGCGCGCGCAGGGCATCCGCGACTTCAAGACGTTCCTGAAGGTGCAT
CCGGAGTTCGTCACGCGCTGCATGCAGGAGATCCGCGTCATCGACGTGAACCAGCAGACGCTGGAGATGTTCGGCGCCGA
CAGCAAGGAGCAACTGCTCCAGCAGATCGGCAAGGTGTTCCGCGGGGAGATGGCCGAGTCCTTCGCCGAGCAGCTGCTCG
ACCTGTGGGACGGCCGGCTGGCGCAGCAGCGCGAGGTCGTGAACTACAGCCTCTCGGGCGAGCCGGTGTATATCCACATG
CAGTTCGCCGTGCTGGACGAACGCAGGCATGACTGGTCCATGGTGCTGCTGTCGCTGGTGGACATCACGGCGCGCAAGAA
GGCGGAGGCCTATCTGGAGTACCTCGGCAAGCACGATGTGCTCACCCAGTTGCGTAATCGCGCCTTCTACATGGAGGAAA
TGAACCGGCTCGTCCGCAAGGGCCCCTGGCCGGTGGCGGTGGTGGTGGTGGATATGAACGGGCTCAAGGCCATCAACGAC
GAGTACGGCCATGCGGCCGGCGATGCGATGCTGCGACGCGTGGGCGAGGTGCTCCTCAAGGCGGTGGACTCCCCGGACTG
CGCCGCGCGCATCGGCGGCGATGAATTCGCCCTGCTGCTGCCGGCCTGCGACGAGCGCGGCGCACAGGCCGTCACCGACC
GGATCGCATCGCTGCTGGAGCTCAACAACCAGTTCTATCCCGGCAAGCCGCTGAGCCTGGCGATCGGGGCCGCCATCGCG
GCCACGGGCGACCAGGTCGAGGGGGCCGTGCACCGCGCCGACCAGGCCATGTACGCGGAGAAGCTGCGCTACTACCAGCA
GGCCGGGCTGGAGCGCCGCCGCAGCTGA

Upstream 100 bases:

>100_bases
CGTTCGCGCCGCACGATGCGCGCAAGCGCCCGGCGCGTCCGGCGCGCTGAGGTCCGCATGACCGCAAAGGCCAGCCCTCG
AGGCTGGCTTTTTTTCGTCC

Downstream 100 bases:

>100_bases
CGCGGGCACCTCCGGCCGCGAGCACGGGCGCACGAAGCTGCCACGGTGCGGCAGGCCGCGCCTGCTTCAGGCGGCGGTGG
CCACGCGGCCGATGTCCGGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MPRRGACRTRVRPIIHLPRAHTHGVLRKMTFPPSSENDFEHMFDLAPVSLWLEDFSALKSLLDAWRSEGVVDAEAYVRED
PARMQQYGASLKVLQVNRRTLELFAAPDQATLLASLDQVFRGDMFEQAISELRQLWSGSLEFSNQTVNYALDGRRLDVHI
RGRILPGHEDRWDRVLISLEDNTEQIRSGTALLRSEQYARGLFEHSPVSLWVEDFSCVKRLLDDVRAQGIRDFKTFLKVH
PEFVTRCMQEIRVIDVNQQTLEMFGADSKEQLLQQIGKVFRGEMAESFAEQLLDLWDGRLAQQREVVNYSLSGEPVYIHM
QFAVLDERRHDWSMVLLSLVDITARKKAEAYLEYLGKHDVLTQLRNRAFYMEEMNRLVRKGPWPVAVVVVDMNGLKAIND
EYGHAAGDAMLRRVGEVLLKAVDSPDCAARIGGDEFALLLPACDERGAQAVTDRIASLLELNNQFYPGKPLSLAIGAAIA
ATGDQVEGAVHRADQAMYAEKLRYYQQAGLERRRS

Sequences:

>Translated_515_residues
MPRRGACRTRVRPIIHLPRAHTHGVLRKMTFPPSSENDFEHMFDLAPVSLWLEDFSALKSLLDAWRSEGVVDAEAYVRED
PARMQQYGASLKVLQVNRRTLELFAAPDQATLLASLDQVFRGDMFEQAISELRQLWSGSLEFSNQTVNYALDGRRLDVHI
RGRILPGHEDRWDRVLISLEDNTEQIRSGTALLRSEQYARGLFEHSPVSLWVEDFSCVKRLLDDVRAQGIRDFKTFLKVH
PEFVTRCMQEIRVIDVNQQTLEMFGADSKEQLLQQIGKVFRGEMAESFAEQLLDLWDGRLAQQREVVNYSLSGEPVYIHM
QFAVLDERRHDWSMVLLSLVDITARKKAEAYLEYLGKHDVLTQLRNRAFYMEEMNRLVRKGPWPVAVVVVDMNGLKAIND
EYGHAAGDAMLRRVGEVLLKAVDSPDCAARIGGDEFALLLPACDERGAQAVTDRIASLLELNNQFYPGKPLSLAIGAAIA
ATGDQVEGAVHRADQAMYAEKLRYYQQAGLERRRS
>Mature_514_residues
PRRGACRTRVRPIIHLPRAHTHGVLRKMTFPPSSENDFEHMFDLAPVSLWLEDFSALKSLLDAWRSEGVVDAEAYVREDP
ARMQQYGASLKVLQVNRRTLELFAAPDQATLLASLDQVFRGDMFEQAISELRQLWSGSLEFSNQTVNYALDGRRLDVHIR
GRILPGHEDRWDRVLISLEDNTEQIRSGTALLRSEQYARGLFEHSPVSLWVEDFSCVKRLLDDVRAQGIRDFKTFLKVHP
EFVTRCMQEIRVIDVNQQTLEMFGADSKEQLLQQIGKVFRGEMAESFAEQLLDLWDGRLAQQREVVNYSLSGEPVYIHMQ
FAVLDERRHDWSMVLLSLVDITARKKAEAYLEYLGKHDVLTQLRNRAFYMEEMNRLVRKGPWPVAVVVVDMNGLKAINDE
YGHAAGDAMLRRVGEVLLKAVDSPDCAARIGGDEFALLLPACDERGAQAVTDRIASLLELNNQFYPGKPLSLAIGAAIAA
TGDQVEGAVHRADQAMYAEKLRYYQQAGLERRRS

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=210, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI145693134, Length=152, Percent_Identity=36.1842105263158, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=30.1775147928994, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787262, Length=153, Percent_Identity=28.1045751633987, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=30.635838150289, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788085, Length=153, Percent_Identity=28.1045751633987, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081974, Length=173, Percent_Identity=27.1676300578035, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=29.8850574712644, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=30.9090909090909, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788956, Length=149, Percent_Identity=30.8724832214765, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013655 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF05231 MASE1; PF08447 PAS_3; PF08448 PAS_4 [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 58602; Mature: 58470

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRGACRTRVRPIIHLPRAHTHGVLRKMTFPPSSENDFEHMFDLAPVSLWLEDFSALKS
CCCCCCCHHHCCHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLDAWRSEGVVDAEAYVREDPARMQQYGASLKVLQVNRRTLELFAAPDQATLLASLDQVF
HHHHHHHCCCCCHHHHHHCCHHHHHHHCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHH
RGDMFEQAISELRQLWSGSLEFSNQTVNYALDGRRLDVHIRGRILPGHEDRWDRVLISLE
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEEEECCCCCCCCCEEEEEEC
DNTEQIRSGTALLRSEQYARGLFEHSPVSLWVEDFSCVKRLLDDVRAQGIRDFKTFLKVH
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
PEFVTRCMQEIRVIDVNQQTLEMFGADSKEQLLQQIGKVFRGEMAESFAEQLLDLWDGRL
HHHHHHHHHHCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AQQREVVNYSLSGEPVYIHMQFAVLDERRHDWSMVLLSLVDITARKKAEAYLEYLGKHDV
HHHHHHHEEECCCCEEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LTQLRNRAFYMEEMNRLVRKGPWPVAVVVVDMNGLKAINDEYGHAAGDAMLRRVGEVLLK
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHCHHCCCHHHHHHHHHHHHHHHH
AVDSPDCAARIGGDEFALLLPACDERGAQAVTDRIASLLELNNQFYPGKPLSLAIGAAIA
HCCCCCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
ATGDQVEGAVHRADQAMYAEKLRYYQQAGLERRRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRRGACRTRVRPIIHLPRAHTHGVLRKMTFPPSSENDFEHMFDLAPVSLWLEDFSALKS
CCCCCCHHHCCHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLDAWRSEGVVDAEAYVREDPARMQQYGASLKVLQVNRRTLELFAAPDQATLLASLDQVF
HHHHHHHCCCCCHHHHHHCCHHHHHHHCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHH
RGDMFEQAISELRQLWSGSLEFSNQTVNYALDGRRLDVHIRGRILPGHEDRWDRVLISLE
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEEEECCCCCCCCCEEEEEEC
DNTEQIRSGTALLRSEQYARGLFEHSPVSLWVEDFSCVKRLLDDVRAQGIRDFKTFLKVH
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
PEFVTRCMQEIRVIDVNQQTLEMFGADSKEQLLQQIGKVFRGEMAESFAEQLLDLWDGRL
HHHHHHHHHHCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AQQREVVNYSLSGEPVYIHMQFAVLDERRHDWSMVLLSLVDITARKKAEAYLEYLGKHDV
HHHHHHHEEECCCCEEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LTQLRNRAFYMEEMNRLVRKGPWPVAVVVVDMNGLKAINDEYGHAAGDAMLRRVGEVLLK
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHCHHCCCHHHHHHHHHHHHHHHH
AVDSPDCAARIGGDEFALLLPACDERGAQAVTDRIASLLELNNQFYPGKPLSLAIGAAIA
HCCCCCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
ATGDQVEGAVHRADQAMYAEKLRYYQQAGLERRRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 6094528; 7984428 [H]