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The map label for this gene is trmD

Identifier: 120610566

GI number: 120610566

Start: 2042193

End: 2042960

Strand: Direct

Name: trmD

Synonym: Aave_1886

Alternate gene names: 120610566

Gene position: 2042193-2042960 (Clockwise)

Preceding gene: 120610565

Following gene: 120610567

Centisome position: 38.15

GC content: 71.88

Gene sequence:

>768_bases
ATGCGTTTCGACGTCATCACGCTGTTCCCGGAGCTGTTCACGCCGTTTCTCGCCAGCGGCGTGACGCGACGGGCCTATGC
CACCGGGCTCGTGGACGTGCGCTTCTGGAACCCGCGCGACTACGCGGAGGGCAACTACCGCCGCGTGGACGACCGCCCCT
TCGGCGGAGGGCCGGGCATGGTCATGATGGCCGAGCCGCTCGAGCGCTGCCTGCGGGCCATCCGCGCGGACCGCGGCGAG
GCGCCCGAGGCCGTGGCGCCACTGGTGATGTTCTCGCCGATCGGGCGCAGGCTGGACCATGACGGCGTGGCGGGCTGGGC
CGCCGGCAACGGCGCAGTGCTGCTGTGCGGCCGCTACGAGGGCGTGGACCAGCGGTTCGTCGATGCCCATGTGGACGTGC
AGCTGAGCCTGGGGGATTTCGTCCTCTCCGGCGGCGAGATCCCCGCCATGGCGCTGCTCGACGCGGTGGCGCGGCTGCAG
CCCGGCGTGCTGAACGATGCCGGCAGCCACCAGGAAGACAGCTTCAATGCAGCGCTGGACGGCCTGCTGGACTGTCCGCA
CTACACCCGTCCGGAGTCGTGGCAGGGGCAGGCGGTGCCCGCCGCGCTGCTGTCCGGCCACCATGCGCAGATCGAGCGCT
GGCGGCGCGACCAGCGGCTTGCCGTGACGGCGCGGCACCGGCCGGACCTGGTCGAGGCCGCGCGCGTGGCGGGGCGCCTC
GACCGCGCGGATGAAGCCGTCTTGGCGAAACTGAACGGCTTGCTATAA

Upstream 100 bases:

>100_bases
GGGCGAGCGCATGATTCCCTTCGTGTCCGCCTTCGTCGATCGGGTGGACATCGCCGGGCGCCGCATCGTGGTGGACTGGC
AGCCGGATTACTGAGCCGCC

Downstream 100 bases:

>100_bases
TGGCGGGCTTTCCGATCCTCTGTCCGGCCGCTGGCGGACGCTTCCTTTTCGCAGCCGCAGGGCTGCCGGGGCGACCTTTC
AGCACCATGCGTCAATCCCG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRFDVITLFPELFTPFLASGVTRRAYATGLVDVRFWNPRDYAEGNYRRVDDRPFGGGPGMVMMAEPLERCLRAIRADRGE
APEAVAPLVMFSPIGRRLDHDGVAGWAAGNGAVLLCGRYEGVDQRFVDAHVDVQLSLGDFVLSGGEIPAMALLDAVARLQ
PGVLNDAGSHQEDSFNAALDGLLDCPHYTRPESWQGQAVPAALLSGHHAQIERWRRDQRLAVTARHRPDLVEAARVAGRL
DRADEAVLAKLNGLL

Sequences:

>Translated_255_residues
MRFDVITLFPELFTPFLASGVTRRAYATGLVDVRFWNPRDYAEGNYRRVDDRPFGGGPGMVMMAEPLERCLRAIRADRGE
APEAVAPLVMFSPIGRRLDHDGVAGWAAGNGAVLLCGRYEGVDQRFVDAHVDVQLSLGDFVLSGGEIPAMALLDAVARLQ
PGVLNDAGSHQEDSFNAALDGLLDCPHYTRPESWQGQAVPAALLSGHHAQIERWRRDQRLAVTARHRPDLVEAARVAGRL
DRADEAVLAKLNGLL
>Mature_255_residues
MRFDVITLFPELFTPFLASGVTRRAYATGLVDVRFWNPRDYAEGNYRRVDDRPFGGGPGMVMMAEPLERCLRAIRADRGE
APEAVAPLVMFSPIGRRLDHDGVAGWAAGNGAVLLCGRYEGVDQRFVDAHVDVQLSLGDFVLSGGEIPAMALLDAVARLQ
PGVLNDAGSHQEDSFNAALDGLLDCPHYTRPESWQGQAVPAALLSGHHAQIERWRRDQRLAVTARHRPDLVEAARVAGRL
DRADEAVLAKLNGLL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=232, Percent_Identity=48.7068965517241, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ACIAC (A1TND2)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970244.1
- ProteinModelPortal:   A1TND2
- SMR:   A1TND2
- STRING:   A1TND2
- GeneID:   4669343
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1886
- NMPDR:   fig|397945.5.peg.1634
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   A1TND2
- ProtClustDB:   PRK00026
- BioCyc:   AAVE397945:AAVE_1886-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27889; Mature: 27889

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDVITLFPELFTPFLASGVTRRAYATGLVDVRFWNPRDYAEGNYRRVDDRPFGGGPGM
CCCCHHHHHHHHHHHHHHHCHHHHHHHHCEEEEEEECCCCCCCCCCEECCCCCCCCCCCE
VMMAEPLERCLRAIRADRGEAPEAVAPLVMFSPIGRRLDHDGVAGWAAGNGAVLLCGRYE
EEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCC
GVDQRFVDAHVDVQLSLGDFVLSGGEIPAMALLDAVARLQPGVLNDAGSHQEDSFNAALD
CCCHHHHHEEEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
GLLDCPHYTRPESWQGQAVPAALLSGHHAQIERWRRDQRLAVTARHRPDLVEAARVAGRL
HHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
DRADEAVLAKLNGLL
CCHHHHHHHHHHCCC
>Mature Secondary Structure
MRFDVITLFPELFTPFLASGVTRRAYATGLVDVRFWNPRDYAEGNYRRVDDRPFGGGPGM
CCCCHHHHHHHHHHHHHHHCHHHHHHHHCEEEEEEECCCCCCCCCCEECCCCCCCCCCCE
VMMAEPLERCLRAIRADRGEAPEAVAPLVMFSPIGRRLDHDGVAGWAAGNGAVLLCGRYE
EEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCC
GVDQRFVDAHVDVQLSLGDFVLSGGEIPAMALLDAVARLQPGVLNDAGSHQEDSFNAALD
CCCHHHHHEEEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
GLLDCPHYTRPESWQGQAVPAALLSGHHAQIERWRRDQRLAVTARHRPDLVEAARVAGRL
HHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
DRADEAVLAKLNGLL
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA