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The map label for this gene is prfB [H]

Identifier: 120610527

GI number: 120610527

Start: 2003534

End: 2004436

Strand: Reverse

Name: prfB [H]

Synonym: Aave_1847

Alternate gene names: 120610527

Gene position: 2004436-2003534 (Counterclockwise)

Preceding gene: 120610528

Following gene: 120610526

Centisome position: 37.45

GC content: 65.56

Gene sequence:

>903_bases
GTGGTCGTCACGCTCGACAAGCTCACGCGCGAGCTGGCGGACAACGCCGAGCTCTATGAGATGAGCAAGGAAGAAGGCGA
CGAGGCCGGCCTGCTCACCATCGAGGCCGAGGCCGGCAAGCTGCGTCCGCTCATCGAGGAGCTGGAGTTCCGCCGCATGT
TCAGCAACGAGGCCGATCCGCTGAACTGCTTCGTCGATATCCAGGCCGGCGCCGGCGGCACCGAGGCCTGCGACTGGGCC
GGCATGCTGCTGCGCCAGTACCTCAAGTACGCCGAGCGCAAGGGCTTCAAGGCCACGGTGGAAGACGAGACCCCGGGCGA
CGTGGCCGGCATCAAGAGCGCGACGATCAAGATCGAAGGCGAGTACGCCTACGGCCTGCTGCGCACGGAAACCGGCGTGC
ACCGCCTCGTGCGCAAGAGCCCTTTCGATTCCTCCGGCGGCCGCCACACGAGCTTCGCCTCGCTGTTCGTCTATCCCGAG
ATCGACGACTCGATCGAGATCAACATCAATCCGGCCGACGTGCGCACCGACACCTTCCGCGCATCGGGCGCGGGCGGGCA
GCACATCAACAAGACCGATTCGGCCGTGCGCCTGACGCACATCCCGACCGGCATCGTCGTGCAGTGCCAGGACGGCCGCA
GCCAGCACAGCAACCGCGATGTCGCCTGGCAGCGCCTGCGCTCCCGCCTCTACGACTTCGAGATGCGCAAGCGCATGGAA
GAGCAGCAGAAGCTGGAAGACACGAAGACCGACGTGGGCTGGGGCCACCAGATCCGCAGCTACGTGCTCGACAACAGCCG
CATCAAGGACCTGCGCACCAACGTCGAAGTCTCCGCCACCCAGAAGGTGCTGGACGGCGACCTCGACGTGTTTATCGAAG
CTTCCCTCAAGCAGGGCGTCTGA

Upstream 100 bases:

>100_bases
ACGCCTGCGCACGGTAAACGCCTCCCTCGAAGATCCGTCCGTCTGGAACGATCCCAAGAAGGCCCAGGAACTCGGCAAGG
AACAGAAGTCGCTCTCCGCG

Downstream 100 bases:

>100_bases
GGCCACCATGAAGCTGACTCGCGTGGAGGCACGCCATTGTTTCAGTCTGGAAGACGTGGGCGTAGACCTGTCTCCTGGTG
TGACGGTGCTAGTAGGCCCC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MVVTLDKLTRELADNAELYEMSKEEGDEAGLLTIEAEAGKLRPLIEELEFRRMFSNEADPLNCFVDIQAGAGGTEACDWA
GMLLRQYLKYAERKGFKATVEDETPGDVAGIKSATIKIEGEYAYGLLRTETGVHRLVRKSPFDSSGGRHTSFASLFVYPE
IDDSIEININPADVRTDTFRASGAGGQHINKTDSAVRLTHIPTGIVVQCQDGRSQHSNRDVAWQRLRSRLYDFEMRKRME
EQQKLEDTKTDVGWGHQIRSYVLDNSRIKDLRTNVEVSATQKVLDGDLDVFIEASLKQGV

Sequences:

>Translated_300_residues
MVVTLDKLTRELADNAELYEMSKEEGDEAGLLTIEAEAGKLRPLIEELEFRRMFSNEADPLNCFVDIQAGAGGTEACDWA
GMLLRQYLKYAERKGFKATVEDETPGDVAGIKSATIKIEGEYAYGLLRTETGVHRLVRKSPFDSSGGRHTSFASLFVYPE
IDDSIEININPADVRTDTFRASGAGGQHINKTDSAVRLTHIPTGIVVQCQDGRSQHSNRDVAWQRLRSRLYDFEMRKRME
EQQKLEDTKTDVGWGHQIRSYVLDNSRIKDLRTNVEVSATQKVLDGDLDVFIEASLKQGV
>Mature_300_residues
MVVTLDKLTRELADNAELYEMSKEEGDEAGLLTIEAEAGKLRPLIEELEFRRMFSNEADPLNCFVDIQAGAGGTEACDWA
GMLLRQYLKYAERKGFKATVEDETPGDVAGIKSATIKIEGEYAYGLLRTETGVHRLVRKSPFDSSGGRHTSFASLFVYPE
IDDSIEININPADVRTDTFRASGAGGQHINKTDSAVRLTHIPTGIVVQCQDGRSQHSNRDVAWQRLRSRLYDFEMRKRME
EQQKLEDTKTDVGWGHQIRSYVLDNSRIKDLRTNVEVSATQKVLDGDLDVFIEASLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=247, Percent_Identity=35.2226720647773, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI34577120, Length=296, Percent_Identity=35.8108108108108, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=38.9261744966443, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI2367172, Length=300, Percent_Identity=62, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=41.5637860082305, Blast_Score=191, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=257, Percent_Identity=34.2412451361868, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6321295, Length=279, Percent_Identity=36.9175627240143, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI19921226, Length=209, Percent_Identity=41.1483253588517, Blast_Score=144, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 33637; Mature: 33637

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVTLDKLTRELADNAELYEMSKEEGDEAGLLTIEAEAGKLRPLIEELEFRRMFSNEADP
CEEEHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
LNCFVDIQAGAGGTEACDWAGMLLRQYLKYAERKGFKATVEDETPGDVAGIKSATIKIEG
EEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEEC
EYAYGLLRTETGVHRLVRKSPFDSSGGRHTSFASLFVYPEIDDSIEININPADVRTDTFR
CEEEEEEECHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECHHHCCCCCEE
ASGAGGQHINKTDSAVRLTHIPTGIVVQCQDGRSQHSNRDVAWQRLRSRLYDFEMRKRME
ECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQQKLEDTKTDVGWGHQIRSYVLDNSRIKDLRTNVEVSATQKVLDGDLDVFIEASLKQGV
HHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCEEEEEECHHCCC
>Mature Secondary Structure
MVVTLDKLTRELADNAELYEMSKEEGDEAGLLTIEAEAGKLRPLIEELEFRRMFSNEADP
CEEEHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
LNCFVDIQAGAGGTEACDWAGMLLRQYLKYAERKGFKATVEDETPGDVAGIKSATIKIEG
EEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEEC
EYAYGLLRTETGVHRLVRKSPFDSSGGRHTSFASLFVYPEIDDSIEININPADVRTDTFR
CEEEEEEECHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECHHHCCCCCEE
ASGAGGQHINKTDSAVRLTHIPTGIVVQCQDGRSQHSNRDVAWQRLRSRLYDFEMRKRME
ECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQQKLEDTKTDVGWGHQIRSYVLDNSRIKDLRTNVEVSATQKVLDGDLDVFIEASLKQGV
HHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCEEEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA