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The map label for this gene is rnhB

Identifier: 120610517

GI number: 120610517

Start: 1991321

End: 1992043

Strand: Direct

Name: rnhB

Synonym: Aave_1837

Alternate gene names: 120610517

Gene position: 1991321-1992043 (Clockwise)

Preceding gene: 120610516

Following gene: 120610518

Centisome position: 37.2

GC content: 71.65

Gene sequence:

>723_bases
ATGCGATCCAGAAAATCCTTGCCGCGCGTTGAGCAGGCCCATCTGCCATGGCATCCGCCGGGACTCGTCGCGGGCGTGGA
CGAGGCGGGCCGCGGGCCGCTGGCCGGGCCCGTGGTGGCCGCGGCCGTCATCCTCGACGAATTGCAGCCCATCGAGGGCC
TGGCGGACTCCAAGACACTCACCGCGGCCCGCCGCGAGGCGCTGTTCGACGAAATCCGCGCCAAGGCGCTGTGCTGCAGC
GTCGCCGAGGCCACGGTGGAAGAGATCGACACGCTGAACATCCTGCAGGCCACCATGCTGGCGATGCGCCGCGCCGTGGC
CGGCCTGCGCCTCAAGCCGGTGCGCGTGCTCGTGGACGGCAACCGCCTGCCGCCGCTGGACGTGCCCGCCGAGGCCATCG
TCAAGGGCGACGCGCTGGTGCAGGCCATCTCCGCCGCATCCATCCTGGCCAAGGTCACGCGCGACCGCTGGTGCGCGCAA
CTGCACGAGGCCTACCCGCACTACGGCTTCGCCTCGCACAAGGGCTATGGCACGGCGGAGCACATGGCGGCCCTGCAGGC
CCATGGCGCATGCCCGGAACACCGTCGCTCCTTCGCACCGGTCGCTGCGGCGGTGCAGCGCACCGTGGTCATGCAGGCCA
CCGCCACCATCGTGACGACCGTGGAAACCACCGTCTCCGTACGCCCCGCGGCGCCCCGGGCCGCGGAAGGGCTGCACGCA
TGA

Upstream 100 bases:

>100_bases
CAGTGGCTGGACGCACCAGCCGGCCAGCGCGACGCGCTGGCGCGGCGCTTCACCGCGCTGCACGAGGAACTGCGCCGCGA
TACCCCGAGATTGGCCGCCG

Downstream 100 bases:

>100_bases
CCGCCGGGCCGACCTTCATCCAGTCGCGCGACAACGCATTCGTGAAGGACCTGCGGCGGCTGGCGCAGGACAGCGGCGCC
TACCGCCGCCAGGGCCGCGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRSRKSLPRVEQAHLPWHPPGLVAGVDEAGRGPLAGPVVAAAVILDELQPIEGLADSKTLTAARREALFDEIRAKALCCS
VAEATVEEIDTLNILQATMLAMRRAVAGLRLKPVRVLVDGNRLPPLDVPAEAIVKGDALVQAISAASILAKVTRDRWCAQ
LHEAYPHYGFASHKGYGTAEHMAALQAHGACPEHRRSFAPVAAAVQRTVVMQATATIVTTVETTVSVRPAAPRAAEGLHA

Sequences:

>Translated_240_residues
MRSRKSLPRVEQAHLPWHPPGLVAGVDEAGRGPLAGPVVAAAVILDELQPIEGLADSKTLTAARREALFDEIRAKALCCS
VAEATVEEIDTLNILQATMLAMRRAVAGLRLKPVRVLVDGNRLPPLDVPAEAIVKGDALVQAISAASILAKVTRDRWCAQ
LHEAYPHYGFASHKGYGTAEHMAALQAHGACPEHRRSFAPVAAAVQRTVVMQATATIVTTVETTVSVRPAAPRAAEGLHA
>Mature_240_residues
MRSRKSLPRVEQAHLPWHPPGLVAGVDEAGRGPLAGPVVAAAVILDELQPIEGLADSKTLTAARREALFDEIRAKALCCS
VAEATVEEIDTLNILQATMLAMRRAVAGLRLKPVRVLVDGNRLPPLDVPAEAIVKGDALVQAISAASILAKVTRDRWCAQ
LHEAYPHYGFASHKGYGTAEHMAALQAHGACPEHRRSFAPVAAAVQRTVVMQATATIVTTVETTVSVRPAAPRAAEGLHA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=62.5, Blast_Score=213, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_ACIAC (A1TN83)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970195.1
- ProteinModelPortal:   A1TN83
- SMR:   A1TN83
- STRING:   A1TN83
- GeneID:   4665935
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1837
- NMPDR:   fig|397945.5.peg.1590
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   IICGVDE
- PhylomeDB:   A1TN83
- ProtClustDB:   PRK00015
- BioCyc:   AAVE397945:AAVE_1837-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 25466; Mature: 25466

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRKSLPRVEQAHLPWHPPGLVAGVDEAGRGPLAGPVVAAAVILDELQPIEGLADSKTL
CCCCCCCCCHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
TAARREALFDEIRAKALCCSVAEATVEEIDTLNILQATMLAMRRAVAGLRLKPVRVLVDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
NRLPPLDVPAEAIVKGDALVQAISAASILAKVTRDRWCAQLHEAYPHYGFASHKGYGTAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
HMAALQAHGACPEHRRSFAPVAAAVQRTVVMQATATIVTTVETTVSVRPAAPRAAEGLHA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRSRKSLPRVEQAHLPWHPPGLVAGVDEAGRGPLAGPVVAAAVILDELQPIEGLADSKTL
CCCCCCCCCHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
TAARREALFDEIRAKALCCSVAEATVEEIDTLNILQATMLAMRRAVAGLRLKPVRVLVDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
NRLPPLDVPAEAIVKGDALVQAISAASILAKVTRDRWCAQLHEAYPHYGFASHKGYGTAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
HMAALQAHGACPEHRRSFAPVAAAVQRTVVMQATATIVTTVETTVSVRPAAPRAAEGLHA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA