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The map label for this gene is ispDF [H]

Identifier: 120610267

GI number: 120610267

Start: 1748080

End: 1748577

Strand: Direct

Name: ispDF [H]

Synonym: Aave_1582

Alternate gene names: 120610267

Gene position: 1748080-1748577 (Clockwise)

Preceding gene: 120610266

Following gene: 120610270

Centisome position: 32.66

GC content: 71.49

Gene sequence:

>498_bases
ATGACGTTTCCCCCATTCCGCATCGGCGAGGGCTGGGACATCCATGCCCTGGTGCCGGGCCGCCGCCTGGTGATCGGCGG
CGTGGACATTCCCCACTCCATGGGCCTGCTCGGGCATTCCGATGCCGACGTGCTGCTGCACGCCGTGACCGACGCGCTGC
TGGGCGCCGTGGGGCTCGGCGACATCGGCAGCCATTTCCCGGACACGGACGAGCGCTTCAAGGGCGCGGATTCCATGGTC
CTGCTGGTGGAAGCCGCGCGGCGCGTGCGCGAGCGCGGCCACGAGATCGGCAACATCGACAGCACGGTGATCGCGCAGGC
CCCGCGCCTCGCGCCGCACATCCCCGCGATGCGCGAAGGCATGGCCCGGTCGCTGGGCCTGCAGCCCGATCAGGTGAACG
TGAAGGCCAAGACGGCCGAGCGCATGGGCCCCGTGGGGCAGGGCCTGGCCATGGAGGCGCGCGCGGCCGTGCTGCTGTTC
AGGCCTTCGCCGGCTTGA

Upstream 100 bases:

>100_bases
CTGCCGGCCACCCTGGAGCGCTTCGGCGGGCCGCTCGCGCAGGGCGCGCCGCTGCCGGGCAAGACTTTCTTCTGACAAGA
CACACAAGGCGGAACTGCCC

Downstream 100 bases:

>100_bases
CGGGCTTGCCGCCCTTGGCCGCGTCGGGCGGCAGCTGGCGCTTGATCTGCGGGCGCTGCAGCCGCTGCTTGGGGTCCTTG
CCGGCCGGCAGCTGCGAGGC

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MTFPPFRIGEGWDIHALVPGRRLVIGGVDIPHSMGLLGHSDADVLLHAVTDALLGAVGLGDIGSHFPDTDERFKGADSMV
LLVEAARRVRERGHEIGNIDSTVIAQAPRLAPHIPAMREGMARSLGLQPDQVNVKAKTAERMGPVGQGLAMEARAAVLLF
RPSPA

Sequences:

>Translated_165_residues
MTFPPFRIGEGWDIHALVPGRRLVIGGVDIPHSMGLLGHSDADVLLHAVTDALLGAVGLGDIGSHFPDTDERFKGADSMV
LLVEAARRVRERGHEIGNIDSTVIAQAPRLAPHIPAMREGMARSLGLQPDQVNVKAKTAERMGPVGQGLAMEARAAVLLF
RPSPA
>Mature_164_residues
TFPPFRIGEGWDIHALVPGRRLVIGGVDIPHSMGLLGHSDADVLLHAVTDALLGAVGLGDIGSHFPDTDERFKGADSMVL
LVEAARRVRERGHEIGNIDSTVIAQAPRLAPHIPAMREGMARSLGLQPDQVNVKAKTAERMGPVGQGLAMEARAAVLLFR
PSPA

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=54.7770700636943, Blast_Score=164, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 17546; Mature: 17414

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPPFRIGEGWDIHALVPGRRLVIGGVDIPHSMGLLGHSDADVLLHAVTDALLGAVGLG
CCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
DIGSHFPDTDERFKGADSMVLLVEAARRVRERGHEIGNIDSTVIAQAPRLAPHIPAMREG
CCCCCCCCCHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
MARSLGLQPDQVNVKAKTAERMGPVGQGLAMEARAAVLLFRPSPA
HHHHHCCCCCCEEEEEHHHHHCCCCCCCCEECCCEEEEEECCCCC
>Mature Secondary Structure 
TFPPFRIGEGWDIHALVPGRRLVIGGVDIPHSMGLLGHSDADVLLHAVTDALLGAVGLG
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
DIGSHFPDTDERFKGADSMVLLVEAARRVRERGHEIGNIDSTVIAQAPRLAPHIPAMREG
CCCCCCCCCHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
MARSLGLQPDQVNVKAKTAERMGPVGQGLAMEARAAVLLFRPSPA
HHHHHCCCCCCEEEEEHHHHHCCCCCCCCEECCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA