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The map label for this gene is ispDF [H]

Identifier: 120610266

GI number: 120610266

Start: 1747230

End: 1748054

Strand: Direct

Name: ispDF [H]

Synonym: Aave_1581

Alternate gene names: 120610266

Gene position: 1747230-1748054 (Clockwise)

Preceding gene: 120610263

Following gene: 120610267

Centisome position: 32.64

GC content: 75.03

Gene sequence:

>825_bases
ATGAGCGACCTGCTGCCGCCCCGACCGACCCTTCCGCCTCCGGCCACGCCGGGCCGTTTCTGGGCGCTGCTGCCCTGTGC
GGGCACCGGCGCCCGCGCCGTGGCGCCGGGCGCCGCGGCCGGCGTGCCCAAGCAGTACCAGGCCGTGGCCGGACTGCCGC
TCGTGCTGCACACCCTGGCGGCATTCGCGGGCGTGCGGCGCCTGCTGGGCACCCTGGTGGCCGTGGCGCCCGGCGACGCA
TTCTTCGACGCCCATCCTTCGGAGCTGCATTTCGCCGTCGCCTGCGGCGGCGCCACGCGCGCCGATACCGTGCTCGGGGG
CTTGCGCGCGCTGCGCGAGCGCGGCGCGGGGGAGGGGGACTGGGTGCTGGTGCACGACGCGGCGCGCTGCCTGGTGACCA
CCGCGCAGATCGACGCCCTCATCGATGCCTGCGAGCACGACAGCGTGGGCGGCCTGCTGGCCCACAAGCTGGCCGACACG
CTCAAGACGGCCAGCGAAGGCCCGGGCGGCGTGCGCGTCGCCTCCACGGTGGACCGCAGCGACAAATGGCTCGCCCAGAC
CCCGCAGATGTTCCGCCTGGGCACCCTCGAATCCGCGCTCGTGCGGTACGGCGCGCAGGCCACCGACGAGGCCAGCGCCA
TGGAGGCCATGGGCCTGCATCCGCGCCTCGTGCCGGGCGGCGCGCAGAACTTCAAGGTCACCTATCCGGACGACTTCGCG
CTGGCCGAGGCCGTGCTGGCGCAGCGCCTGCTGCCGGCCACCCTGGAGCGCTTCGGCGGGCCGCTCGCGCAGGGCGCGCC
GCTGCCGGGCAAGACTTTCTTCTGA

Upstream 100 bases:

>100_bases
GCGCGGCCATGCAGGCATTCTAGGGCGGCGGCCATTCCGGCGGAGCGCCCGGGGCATGCCGGCCGGCGAGGGCGCGCCGC
GCTCCTACAATGGCCGCCCC

Downstream 100 bases:

>100_bases
CAAGACACACAAGGCGGAACTGCCCATGACGTTTCCCCCATTCCGCATCGGCGAGGGCTGGGACATCCATGCCCTGGTGC
CGGGCCGCCGCCTGGTGATC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSDLLPPRPTLPPPATPGRFWALLPCAGTGARAVAPGAAAGVPKQYQAVAGLPLVLHTLAAFAGVRRLLGTLVAVAPGDA
FFDAHPSELHFAVACGGATRADTVLGGLRALRERGAGEGDWVLVHDAARCLVTTAQIDALIDACEHDSVGGLLAHKLADT
LKTASEGPGGVRVASTVDRSDKWLAQTPQMFRLGTLESALVRYGAQATDEASAMEAMGLHPRLVPGGAQNFKVTYPDDFA
LAEAVLAQRLLPATLERFGGPLAQGAPLPGKTFF

Sequences:

>Translated_274_residues
MSDLLPPRPTLPPPATPGRFWALLPCAGTGARAVAPGAAAGVPKQYQAVAGLPLVLHTLAAFAGVRRLLGTLVAVAPGDA
FFDAHPSELHFAVACGGATRADTVLGGLRALRERGAGEGDWVLVHDAARCLVTTAQIDALIDACEHDSVGGLLAHKLADT
LKTASEGPGGVRVASTVDRSDKWLAQTPQMFRLGTLESALVRYGAQATDEASAMEAMGLHPRLVPGGAQNFKVTYPDDFA
LAEAVLAQRLLPATLERFGGPLAQGAPLPGKTFF
>Mature_273_residues
SDLLPPRPTLPPPATPGRFWALLPCAGTGARAVAPGAAAGVPKQYQAVAGLPLVLHTLAAFAGVRRLLGTLVAVAPGDAF
FDAHPSELHFAVACGGATRADTVLGGLRALRERGAGEGDWVLVHDAARCLVTTAQIDALIDACEHDSVGGLLAHKLADTL
KTASEGPGGVRVASTVDRSDKWLAQTPQMFRLGTLESALVRYGAQATDEASAMEAMGLHPRLVPGGAQNFKVTYPDDFAL
AEAVLAQRLLPATLERFGGPLAQGAPLPGKTFF

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=234, Percent_Identity=40.5982905982906, Blast_Score=141, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 28351; Mature: 28220

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLPPRPTLPPPATPGRFWALLPCAGTGARAVAPGAAAGVPKQYQAVAGLPLVLHTLA
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHCHHHHHHHHH
AFAGVRRLLGTLVAVAPGDAFFDAHPSELHFAVACGGATRADTVLGGLRALRERGAGEGD
HHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
WVLVHDAARCLVTTAQIDALIDACEHDSVGGLLAHKLADTLKTASEGPGGVRVASTVDRS
EEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHCCC
DKWLAQTPQMFRLGTLESALVRYGAQATDEASAMEAMGLHPRLVPGGAQNFKVTYPDDFA
CHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEECCCCCCCCEEECCCHHH
LAEAVLAQRLLPATLERFGGPLAQGAPLPGKTFF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDLLPPRPTLPPPATPGRFWALLPCAGTGARAVAPGAAAGVPKQYQAVAGLPLVLHTLA
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHCHHHHHHHHH
AFAGVRRLLGTLVAVAPGDAFFDAHPSELHFAVACGGATRADTVLGGLRALRERGAGEGD
HHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
WVLVHDAARCLVTTAQIDALIDACEHDSVGGLLAHKLADTLKTASEGPGGVRVASTVDRS
EEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHCCC
DKWLAQTPQMFRLGTLESALVRYGAQATDEASAMEAMGLHPRLVPGGAQNFKVTYPDDFA
CHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEECCCCCCCCEEECCCHHH
LAEAVLAQRLLPATLERFGGPLAQGAPLPGKTFF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA