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The map label for this gene is pilS [H]

Identifier: 120609561

GI number: 120609561

Start: 940935

End: 942641

Strand: Reverse

Name: pilS [H]

Synonym: Aave_0867

Alternate gene names: 120609561

Gene position: 942641-940935 (Counterclockwise)

Preceding gene: 120609562

Following gene: 120609560

Centisome position: 17.61

GC content: 71.35

Gene sequence:

>1707_bases
ATGCCCCAGTGGTCCCATTCCACCCTGCAGGGGCCCGCGGCGGATCCGCCGTTCGCGCGCCTGTGGACGAGCTTCCTCGC
GGGCCGCGTCGTGGTGGCCGTGGCCCTGCTGCTGCTGCAGGGTCTGGGTTTCGCCTTCAACCAGGGGGCCGGTCCGGCCC
TGACGGGGCTGTGCACCCTCTACCTGCTGGCGGCGGTCATGACGCTCGCCATCTCGGGCGAAAAGCCGCCCCCGCCGCAG
GCCGGCATCCGCTGGCTGCCCACCATCGGGGTGGACCTGGCGCTGATCGCCAGCCTGCAGATGCTGCAGTCGGGCGCGAT
GAACTACACACCCCTGTTCGGCCTGCCCATCCTCATGGCGGGCGTGCTGGGCACCCTCACCCTGGCCCTGGGCACGACCG
CGGCCGCCACCCTGCTGCTGCTGGCCTGGACCTGGTGGCTGGGTGGCGCGGGCACGGCCGAATCGACGCAGCGCTACTTC
CAGACGGCGCTGACGGGCACCGGCTACTTCATCGTCGCCTACCTCGTGCACCAATTGGCCACGCGCCTGCACCGCGAGCA
GCAGCTGGCGCTGGAAGGCCAGCTCGCCGCAAAGCTGCAGGAACAGGTGAGCGCGCTCGTGATCGACCACCTCTCCGACG
GCGTGATCGTGGTGGACGGCCGCGACACCGTCCGCCTCGCCAACCCGGCCGCGCTGCTGCTGCTCGGCGAATCGCCCCAG
CCCGCCCCGCCGTTCCCGCTCACGAGCTCGCCGTCGTGGGCCCCCCTGCTCGACATGGCCCGGCAGACCTTCCGCCTGGC
GCAGCCGCAGGGCGCCGATGTGGACATCCTGCAGCCGGGCCAGAGCCCGCTCGCGCTGCACGTGCGGACCTGGCTCACGG
CCTCGCCCCTGATCGCGCTCGACACCGGCGGGGCGCAGATGTGCGTCATGTTCCTGCACGACCTGCGCGAGATGGAGGCG
CGCCTGCGCACCGAGAAGCTGGCCGCCATGGGCCGCATGTCCGCCGCCGTGGCCCACGAGATCCGCAATCCACTGGCCGC
CATCGTGCAGGCCAACGCTCTGCTGGAGGAAGACCTGTCCGACCCCGCCCACCGGCGGCTCGCCCACATGGTGCAGCAGA
ATGCCGCCCGGCTCGCCCGCATCGCGGAAGAGGTGCTGGACATCGCGCGCGTGCAGCACCAGATCGACCATGCCCCGGCC
GGCACGCTGCCGCTGGACGAGTCGGTGGCCCAGGTCTGCCGGGACTGGCAGTCGCACAACCCGCACAGCCGCCGCGCCCA
GCTGTCGCTGCATGCACCGGGCACGCAGGTGGAGTTCGACGCCGACCACCTGCGGCGCGTGCTGGTGAACCTGCTCGACA
ACGCCCTGCGCTACATGGGCTCCGAGCCGGATTCGCTCTGCATCTCCACCTATACCAATGCCGGAGGCCAGGCGCACCTG
CAGGTCTGGAGCGACGGGGCGCCCCTGGACAAGACCGTGGAACGCCACCTGTTCGAGCCGTTCTTCTCGTCCGAGAGCCG
CTCCAGCGGCCTGGGCCTCTATATCTGCCGCGAACTCTGCCAGCGCCATGGCGCCTCCGTCGGCTACCAGCGGCTCACCC
GCGTGCTGGAGCGCGGCGCCATCGGCGGCAACGCATTCACGGTGGCCTTCCGCCGCGCCGCCAGGCCGGGAGGACCGGCC
TCCCTGTTTGACACAATCGTCGTCTGA

Upstream 100 bases:

>100_bases
CTGCGGCGTGCACCTGCCGGCCTCGCAGGCGCTCGCGCACGGCGGGCACACCTACTGCGGCCCGGAGCACCAGCGCCTGG
GCCCCCGCTAGGGCCGCGGC

Downstream 100 bases:

>100_bases
CACCGCCCGACCATGCCGCCATGAATGCCCCCGCTGCCACCATCCTGGTCGTCGATGACGAACCCGACCTGCGCACGCTC
TACGAACTCACGCTGCTGCG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MPQWSHSTLQGPAADPPFARLWTSFLAGRVVVAVALLLLQGLGFAFNQGAGPALTGLCTLYLLAAVMTLAISGEKPPPPQ
AGIRWLPTIGVDLALIASLQMLQSGAMNYTPLFGLPILMAGVLGTLTLALGTTAAATLLLLAWTWWLGGAGTAESTQRYF
QTALTGTGYFIVAYLVHQLATRLHREQQLALEGQLAAKLQEQVSALVIDHLSDGVIVVDGRDTVRLANPAALLLLGESPQ
PAPPFPLTSSPSWAPLLDMARQTFRLAQPQGADVDILQPGQSPLALHVRTWLTASPLIALDTGGAQMCVMFLHDLREMEA
RLRTEKLAAMGRMSAAVAHEIRNPLAAIVQANALLEEDLSDPAHRRLAHMVQQNAARLARIAEEVLDIARVQHQIDHAPA
GTLPLDESVAQVCRDWQSHNPHSRRAQLSLHAPGTQVEFDADHLRRVLVNLLDNALRYMGSEPDSLCISTYTNAGGQAHL
QVWSDGAPLDKTVERHLFEPFFSSESRSSGLGLYICRELCQRHGASVGYQRLTRVLERGAIGGNAFTVAFRRAARPGGPA
SLFDTIVV

Sequences:

>Translated_568_residues
MPQWSHSTLQGPAADPPFARLWTSFLAGRVVVAVALLLLQGLGFAFNQGAGPALTGLCTLYLLAAVMTLAISGEKPPPPQ
AGIRWLPTIGVDLALIASLQMLQSGAMNYTPLFGLPILMAGVLGTLTLALGTTAAATLLLLAWTWWLGGAGTAESTQRYF
QTALTGTGYFIVAYLVHQLATRLHREQQLALEGQLAAKLQEQVSALVIDHLSDGVIVVDGRDTVRLANPAALLLLGESPQ
PAPPFPLTSSPSWAPLLDMARQTFRLAQPQGADVDILQPGQSPLALHVRTWLTASPLIALDTGGAQMCVMFLHDLREMEA
RLRTEKLAAMGRMSAAVAHEIRNPLAAIVQANALLEEDLSDPAHRRLAHMVQQNAARLARIAEEVLDIARVQHQIDHAPA
GTLPLDESVAQVCRDWQSHNPHSRRAQLSLHAPGTQVEFDADHLRRVLVNLLDNALRYMGSEPDSLCISTYTNAGGQAHL
QVWSDGAPLDKTVERHLFEPFFSSESRSSGLGLYICRELCQRHGASVGYQRLTRVLERGAIGGNAFTVAFRRAARPGGPA
SLFDTIVV
>Mature_567_residues
PQWSHSTLQGPAADPPFARLWTSFLAGRVVVAVALLLLQGLGFAFNQGAGPALTGLCTLYLLAAVMTLAISGEKPPPPQA
GIRWLPTIGVDLALIASLQMLQSGAMNYTPLFGLPILMAGVLGTLTLALGTTAAATLLLLAWTWWLGGAGTAESTQRYFQ
TALTGTGYFIVAYLVHQLATRLHREQQLALEGQLAAKLQEQVSALVIDHLSDGVIVVDGRDTVRLANPAALLLLGESPQP
APPFPLTSSPSWAPLLDMARQTFRLAQPQGADVDILQPGQSPLALHVRTWLTASPLIALDTGGAQMCVMFLHDLREMEAR
LRTEKLAAMGRMSAAVAHEIRNPLAAIVQANALLEEDLSDPAHRRLAHMVQQNAARLARIAEEVLDIARVQHQIDHAPAG
TLPLDESVAQVCRDWQSHNPHSRRAQLSLHAPGTQVEFDADHLRRVLVNLLDNALRYMGSEPDSLCISTYTNAGGQAHLQ
VWSDGAPLDKTVERHLFEPFFSSESRSSGLGLYICRELCQRHGASVGYQRLTRVLERGAIGGNAFTVAFRRAARPGGPAS
LFDTIVV

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=347, Percent_Identity=25.0720461095101, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790436, Length=208, Percent_Identity=29.3269230769231, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1786912, Length=218, Percent_Identity=30.2752293577982, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61236; Mature: 61105

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQWSHSTLQGPAADPPFARLWTSFLAGRVVVAVALLLLQGLGFAFNQGAGPALTGLCTL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHH
YLLAAVMTLAISGEKPPPPQAGIRWLPTIGVDLALIASLQMLQSGAMNYTPLFGLPILMA
HHHHHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
GVLGTLTLALGTTAAATLLLLAWTWWLGGAGTAESTQRYFQTALTGTGYFIVAYLVHQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TRLHREQQLALEGQLAAKLQEQVSALVIDHLSDGVIVVDGRDTVRLANPAALLLLGESPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCEEEEEECCCCC
PAPPFPLTSSPSWAPLLDMARQTFRLAQPQGADVDILQPGQSPLALHVRTWLTASPLIAL
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEHHHHHCCCCEEEE
DTGGAQMCVMFLHDLREMEARLRTEKLAAMGRMSAAVAHEIRNPLAAIVQANALLEEDLS
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPAHRRLAHMVQQNAARLARIAEEVLDIARVQHQIDHAPAGTLPLDESVAQVCRDWQSHN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
PHSRRAQLSLHAPGTQVEFDADHLRRVLVNLLDNALRYMGSEPDSLCISTYTNAGGQAHL
CCCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
QVWSDGAPLDKTVERHLFEPFFSSESRSSGLGLYICRELCQRHGASVGYQRLTRVLERGA
EEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
IGGNAFTVAFRRAARPGGPASLFDTIVV
CCCCHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
PQWSHSTLQGPAADPPFARLWTSFLAGRVVVAVALLLLQGLGFAFNQGAGPALTGLCTL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHH
YLLAAVMTLAISGEKPPPPQAGIRWLPTIGVDLALIASLQMLQSGAMNYTPLFGLPILMA
HHHHHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
GVLGTLTLALGTTAAATLLLLAWTWWLGGAGTAESTQRYFQTALTGTGYFIVAYLVHQLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TRLHREQQLALEGQLAAKLQEQVSALVIDHLSDGVIVVDGRDTVRLANPAALLLLGESPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCEEEEEECCCCC
PAPPFPLTSSPSWAPLLDMARQTFRLAQPQGADVDILQPGQSPLALHVRTWLTASPLIAL
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEHHHHHCCCCEEEE
DTGGAQMCVMFLHDLREMEARLRTEKLAAMGRMSAAVAHEIRNPLAAIVQANALLEEDLS
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPAHRRLAHMVQQNAARLARIAEEVLDIARVQHQIDHAPAGTLPLDESVAQVCRDWQSHN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
PHSRRAQLSLHAPGTQVEFDADHLRRVLVNLLDNALRYMGSEPDSLCISTYTNAGGQAHL
CCCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
QVWSDGAPLDKTVERHLFEPFFSSESRSSGLGLYICRELCQRHGASVGYQRLTRVLERGA
EEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
IGGNAFTVAFRRAARPGGPASLFDTIVV
CCCCHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]