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The map label for this gene is ycdT [H]

Identifier: 120609444

GI number: 120609444

Start: 803289

End: 804587

Strand: Reverse

Name: ycdT [H]

Synonym: Aave_0749

Alternate gene names: 120609444

Gene position: 804587-803289 (Counterclockwise)

Preceding gene: 120609445

Following gene: 120609443

Centisome position: 15.03

GC content: 68.9

Gene sequence:

>1299_bases
TTGGATAATGATAAAAATAATAAATATGGAGCGTTTCCAACTTTCTGGGGAAACACGCCGATCAAGACCGGCCCCGGCAC
TTCGCGGCCTGCCGGTGCGGATACCTCCTCCACCTGTCCGTCGTCCATGGTTCTCGATCCCTTCACCCTGCTCGTCATCA
CCGCCGCCCTGGCAACCGCCTCCACGCTGTACCTGGCTTCGGAATGGAGCAGCGTGCGCGAGCGTTCGCTGCTGCTTTGG
AGCGCGGGGTTCGCCGTCATCGCCGTCGGCAGCGCGCTCGCCCTGCTGCGCGCCAGCGGCTTCCTGTTCGTGGGCATCTG
GTTCTCCAACGGCCTGCTGATCGTTGCCCACGCGCTGTTCCTGGGCGGCGTTGCCGCGTTCAGGCGTACCCGGCTGCCGC
GCGCCGGGCGGTGGCTGCTGGCCGCCGTGTGGCTGGCGATGCTGTGGCTGCCCGAGGGGCCCTGGTGGTCCAAGACGATG
CTGGGCGTGCAGTCGCTGCTCATCGCTGCCGTCACCTTGCGCGCCGGCCTGCTGCTGCGCCCGCAGGGCGGCGGCGCTTT
GAGCGTGGGCGCGGCACAGCTGCGCTTCGTCCTGTGGGCGCACGGCCTGTTCTACCTGGCAAAGGCCGCTTCGGCGGTGG
CGCTCGACGCATCCATCCACCTGGCCAGCTTCCGGGGCGTGGTCATCCAGGTCTCGCTGGTGGAAGGCGTGATGGCGATC
ATGCTGCTCGCCATGTCCATGACCGGCACCGAGCGCCACCGCCGCGAGGAACGCATGGCCCAGATCGCCGCCCGCGACCC
GCTCACCGCCCTGGACAACCGCCGCGCGCTCTACCTGCGCGCCCCGGCGCTGCTGCGGCAGGCCTCGCCGGCCAGCCCGG
GCGCCCTGCTGCTGATGGACATCGACAATTTCAAGCAGGTCAACGACCTGCACGGCCACGATGCCGGCGACCGCCTGCTC
ATCACGCTCAGCGACCTGATCCGCAGCACGCTGCCGATCGGCGCGCTGGCCGCCCGGCTGGGCGGGGACGAGTTCGTGAT
CCTGCTGCGCGACGCGTCGCCTTCAGCCGCGCAGGCCCTGGGCCGGGAACTGCGCGAGGCGTTCTCCGAACAGGCGAGCA
CGATGTTCGCCACGCCGGAGCCCGTGTCCCTCAGCATCGGGGCGACCTTGTTCGACCAGCCCGATGCCGAGCTGTCGCAT
TGGCTCAAGCGGGCGGACGAAGCGCTGTATGCGTCGAAGCGCCAGGGGCGGGATCGGATGGAGGTCGTTTTGTCGGATCC
GGCAGGGCGGCTGCATTGA

Upstream 100 bases:

>100_bases
CGCGCCTGCGTGCGGGTGGAGCTGGAAACCGGCGTGCTGGTGGAGTTGCCGGTGGAAGAGCCGCCCCAAAGGCAGTCGCC
TGCACGGGGTTTGAACTCTG

Downstream 100 bases:

>100_bases
TGTGCGCTGGATTGATGGAAACGGCTGTGGCGCGAGACCGGCTTCTTTCAGCTTCAGCGGCGTCGTTCAGGCCGAGATGC
TGCACAGCGAGCGTGCGGCT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MDNDKNNKYGAFPTFWGNTPIKTGPGTSRPAGADTSSTCPSSMVLDPFTLLVITAALATASTLYLASEWSSVRERSLLLW
SAGFAVIAVGSALALLRASGFLFVGIWFSNGLLIVAHALFLGGVAAFRRTRLPRAGRWLLAAVWLAMLWLPEGPWWSKTM
LGVQSLLIAAVTLRAGLLLRPQGGGALSVGAAQLRFVLWAHGLFYLAKAASAVALDASIHLASFRGVVIQVSLVEGVMAI
MLLAMSMTGTERHRREERMAQIAARDPLTALDNRRALYLRAPALLRQASPASPGALLLMDIDNFKQVNDLHGHDAGDRLL
ITLSDLIRSTLPIGALAARLGGDEFVILLRDASPSAAQALGRELREAFSEQASTMFATPEPVSLSIGATLFDQPDAELSH
WLKRADEALYASKRQGRDRMEVVLSDPAGRLH

Sequences:

>Translated_432_residues
MDNDKNNKYGAFPTFWGNTPIKTGPGTSRPAGADTSSTCPSSMVLDPFTLLVITAALATASTLYLASEWSSVRERSLLLW
SAGFAVIAVGSALALLRASGFLFVGIWFSNGLLIVAHALFLGGVAAFRRTRLPRAGRWLLAAVWLAMLWLPEGPWWSKTM
LGVQSLLIAAVTLRAGLLLRPQGGGALSVGAAQLRFVLWAHGLFYLAKAASAVALDASIHLASFRGVVIQVSLVEGVMAI
MLLAMSMTGTERHRREERMAQIAARDPLTALDNRRALYLRAPALLRQASPASPGALLLMDIDNFKQVNDLHGHDAGDRLL
ITLSDLIRSTLPIGALAARLGGDEFVILLRDASPSAAQALGRELREAFSEQASTMFATPEPVSLSIGATLFDQPDAELSH
WLKRADEALYASKRQGRDRMEVVLSDPAGRLH
>Mature_432_residues
MDNDKNNKYGAFPTFWGNTPIKTGPGTSRPAGADTSSTCPSSMVLDPFTLLVITAALATASTLYLASEWSSVRERSLLLW
SAGFAVIAVGSALALLRASGFLFVGIWFSNGLLIVAHALFLGGVAAFRRTRLPRAGRWLLAAVWLAMLWLPEGPWWSKTM
LGVQSLLIAAVTLRAGLLLRPQGGGALSVGAAQLRFVLWAHGLFYLAKAASAVALDASIHLASFRGVVIQVSLVEGVMAI
MLLAMSMTGTERHRREERMAQIAARDPLTALDNRRALYLRAPALLRQASPASPGALLLMDIDNFKQVNDLHGHDAGDRLL
ITLSDLIRSTLPIGALAARLGGDEFVILLRDASPSAAQALGRELREAFSEQASTMFATPEPVSLSIGATLFDQPDAELSH
WLKRADEALYASKRQGRDRMEVVLSDPAGRLH

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=35.5421686746988, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI87081881, Length=181, Percent_Identity=33.1491712707182, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=35.7575757575758, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=35.632183908046, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786584, Length=181, Percent_Identity=30.939226519337, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=31.638418079096, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787541, Length=200, Percent_Identity=32, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=34.3373493975904, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=27.710843373494, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=32.7380952380952, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=34.1935483870968, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081974, Length=171, Percent_Identity=29.2397660818713, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081977, Length=171, Percent_Identity=30.9941520467836, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46457; Mature: 46457

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNDKNNKYGAFPTFWGNTPIKTGPGTSRPAGADTSSTCPSSMVLDPFTLLVITAALATA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
STLYLASEWSSVRERSLLLWSAGFAVIAVGSALALLRASGFLFVGIWFSNGLLIVAHALF
HHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHH
LGGVAAFRRTRLPRAGRWLLAAVWLAMLWLPEGPWWSKTMLGVQSLLIAAVTLRAGLLLR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
PQGGGALSVGAAQLRFVLWAHGLFYLAKAASAVALDASIHLASFRGVVIQVSLVEGVMAI
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEEEECCCEEEEEHHHHHHHHH
MLLAMSMTGTERHRREERMAQIAARDPLTALDNRRALYLRAPALLRQASPASPGALLLMD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECHHHHHCCCCCCCCEEEEEE
IDNFKQVNDLHGHDAGDRLLITLSDLIRSTLPIGALAARLGGDEFVILLRDASPSAAQAL
CCCCHHHHHCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCHHHHHH
GRELREAFSEQASTMFATPEPVSLSIGATLFDQPDAELSHWLKRADEALYASKRQGRDRM
HHHHHHHHHHHHHEEECCCCCCEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
EVVLSDPAGRLH
EEEECCCCCCCC
>Mature Secondary Structure
MDNDKNNKYGAFPTFWGNTPIKTGPGTSRPAGADTSSTCPSSMVLDPFTLLVITAALATA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
STLYLASEWSSVRERSLLLWSAGFAVIAVGSALALLRASGFLFVGIWFSNGLLIVAHALF
HHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHH
LGGVAAFRRTRLPRAGRWLLAAVWLAMLWLPEGPWWSKTMLGVQSLLIAAVTLRAGLLLR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
PQGGGALSVGAAQLRFVLWAHGLFYLAKAASAVALDASIHLASFRGVVIQVSLVEGVMAI
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEEEECCCEEEEEHHHHHHHHH
MLLAMSMTGTERHRREERMAQIAARDPLTALDNRRALYLRAPALLRQASPASPGALLLMD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECHHHHHCCCCCCCCEEEEEE
IDNFKQVNDLHGHDAGDRLLITLSDLIRSTLPIGALAARLGGDEFVILLRDASPSAAQAL
CCCCHHHHHCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCHHHHHH
GRELREAFSEQASTMFATPEPVSLSIGATLFDQPDAELSHWLKRADEALYASKRQGRDRM
HHHHHHHHHHHHHEEECCCCCCEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
EVVLSDPAGRLH
EEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]