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The map label for this gene is gcvA [H]

Identifier: 120609395

GI number: 120609395

Start: 757777

End: 758739

Strand: Reverse

Name: gcvA [H]

Synonym: Aave_0698

Alternate gene names: 120609395

Gene position: 758739-757777 (Counterclockwise)

Preceding gene: 120609399

Following gene: 120609393

Centisome position: 14.17

GC content: 53.69

Gene sequence:

>963_bases
ATGCATCAGCATATCTTCGGGCACATCCCTCTGAGTGCGTTACGTGCTTTCGAAGCGGCGGCGCGACTGCAGAGCTTCAA
GGAAGCGGCGACAGAGCTCTCAATTACCCCAACAGCTATAAGTCACCGGATCAAACAATTGGAAGATGCGCTTGGCGCTC
GTTTGTTCGCGCGCGAGGTACGCAGTGTCAGCCTCACCGATGCTGGGCAACTTTTATATCCTGCAGTCCACACCGCTTTC
ACGGGCATCGTGGGGGCAATTGAGGAGTTGCAACGCCGGGATCGCAGGCCATCCGTCACGTTAAGCACTACATGCGGGTT
CGCCGCTCGTTGGCTTTTACCCCGACTTCCCGCACTGGCGGCGGCTATTCCTAACGTAGATTTGCACCTTCATTCATCTG
ATTCCCCGGTGCAACTTAAAGCTGGCAGTGCAGATCTGGCGATTAGATTTTGCACTTCCCCCGACTCAAGTGTTGGAAGC
GAAGTATTGCTTCCCGGCCGATATGTGCCTGTTTGCGCCCCATCACTGAATGTCAGGTCTGGTGGCGATTTTTCCAATAC
TGCGAGGTTGCGTTTCCGTTGGAGTAAATACGACGAGTGCATCCCGGATTGGCCTCAGTGGCTGGCTGCATCAACTATGG
AAGGACAGCCCTCTGGCCCAGAGCTTGGCTTTTCCGATGAAGCGCACACCATCCAAGCTGCGATTGCGGGTCAAGGAGTT
GCACTTTCAAGTGTTGCCCTTGTTGCAGATGCACTTGATGAAGGGCTGCTTCGTGTGCCGTTCGGTCCCGCAGTGGAGGG
GCGTGATTTCTATTTACTCTCTGCTTTGGATACCTGTGAACAGCCGGCTGTGATAATGGTCAAGAGTTGGCTTAGGGAGG
AAGCACGCGCTTTCGTAGCAAAGCACGCGAGTTATTTTTCAGTCGAATGTCCAAAAATCAGTGACACAGAAAAATTGCCG
TGA

Upstream 100 bases:

>100_bases
CTTTGCATATGTAGTCGTCAAACAGCCGACAAGCGTATGATCTATGTATCCAAGTTTGACGACAAACGATCTTTCCTCAG
TCGATAGGTGACATAAATTC

Downstream 100 bases:

>100_bases
ACTAACTATGACTCCAAAGTCTGTGTCAATTATTTTTTACTGATCAAAGAGTTACGCTGGCCAGTCTTTCGATAGAGGAC
CAAATACGCTTCGCATAGTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MHQHIFGHIPLSALRAFEAAARLQSFKEAATELSITPTAISHRIKQLEDALGARLFAREVRSVSLTDAGQLLYPAVHTAF
TGIVGAIEELQRRDRRPSVTLSTTCGFAARWLLPRLPALAAAIPNVDLHLHSSDSPVQLKAGSADLAIRFCTSPDSSVGS
EVLLPGRYVPVCAPSLNVRSGGDFSNTARLRFRWSKYDECIPDWPQWLAASTMEGQPSGPELGFSDEAHTIQAAIAGQGV
ALSSVALVADALDEGLLRVPFGPAVEGRDFYLLSALDTCEQPAVIMVKSWLREEARAFVAKHASYFSVECPKISDTEKLP

Sequences:

>Translated_320_residues
MHQHIFGHIPLSALRAFEAAARLQSFKEAATELSITPTAISHRIKQLEDALGARLFAREVRSVSLTDAGQLLYPAVHTAF
TGIVGAIEELQRRDRRPSVTLSTTCGFAARWLLPRLPALAAAIPNVDLHLHSSDSPVQLKAGSADLAIRFCTSPDSSVGS
EVLLPGRYVPVCAPSLNVRSGGDFSNTARLRFRWSKYDECIPDWPQWLAASTMEGQPSGPELGFSDEAHTIQAAIAGQGV
ALSSVALVADALDEGLLRVPFGPAVEGRDFYLLSALDTCEQPAVIMVKSWLREEARAFVAKHASYFSVECPKISDTEKLP
>Mature_320_residues
MHQHIFGHIPLSALRAFEAAARLQSFKEAATELSITPTAISHRIKQLEDALGARLFAREVRSVSLTDAGQLLYPAVHTAF
TGIVGAIEELQRRDRRPSVTLSTTCGFAARWLLPRLPALAAAIPNVDLHLHSSDSPVQLKAGSADLAIRFCTSPDSSVGS
EVLLPGRYVPVCAPSLNVRSGGDFSNTARLRFRWSKYDECIPDWPQWLAASTMEGQPSGPELGFSDEAHTIQAAIAGQGV
ALSSVALVADALDEGLLRVPFGPAVEGRDFYLLSALDTCEQPAVIMVKSWLREEARAFVAKHASYFSVECPKISDTEKLP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=32.996632996633, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=31.3131313131313, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=30.3754266211604, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787128, Length=259, Percent_Identity=28.957528957529, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI145693193, Length=258, Percent_Identity=25.5813953488372, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34629; Mature: 34629

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQHIFGHIPLSALRAFEAAARLQSFKEAATELSITPTAISHRIKQLEDALGARLFAREV
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSVSLTDAGQLLYPAVHTAFTGIVGAIEELQRRDRRPSVTLSTTCGFAARWLLPRLPALA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
AAIPNVDLHLHSSDSPVQLKAGSADLAIRFCTSPDSSVGSEVLLPGRYVPVCAPSLNVRS
HHCCCCEEEEECCCCCEEEECCCCEEEEEEECCCCCCCCCCEECCCCCCEEECCCCCCCC
GGDFSNTARLRFRWSKYDECIPDWPQWLAASTMEGQPSGPELGFSDEAHTIQAAIAGQGV
CCCCCCCEEEEEEECHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHEEHHHHCCCCC
ALSSVALVADALDEGLLRVPFGPAVEGRDFYLLSALDTCEQPAVIMVKSWLREEARAFVA
HHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECHHCCCCCHHHHHHHHHHHHHHHHHH
KHASYFSVECPKISDTEKLP
HHCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MHQHIFGHIPLSALRAFEAAARLQSFKEAATELSITPTAISHRIKQLEDALGARLFAREV
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSVSLTDAGQLLYPAVHTAFTGIVGAIEELQRRDRRPSVTLSTTCGFAARWLLPRLPALA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
AAIPNVDLHLHSSDSPVQLKAGSADLAIRFCTSPDSSVGSEVLLPGRYVPVCAPSLNVRS
HHCCCCEEEEECCCCCEEEECCCCEEEEEEECCCCCCCCCCEECCCCCCEEECCCCCCCC
GGDFSNTARLRFRWSKYDECIPDWPQWLAASTMEGQPSGPELGFSDEAHTIQAAIAGQGV
CCCCCCCEEEEEEECHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHEEHHHHCCCCC
ALSSVALVADALDEGLLRVPFGPAVEGRDFYLLSALDTCEQPAVIMVKSWLREEARAFVA
HHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECHHCCCCCHHHHHHHHHHHHHHHHHH
KHASYFSVECPKISDTEKLP
HHCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]