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The map label for this gene is fliY [H]

Identifier: 120609004

GI number: 120609004

Start: 333112

End: 333879

Strand: Reverse

Name: fliY [H]

Synonym: Aave_0301

Alternate gene names: 120609004

Gene position: 333879-333112 (Counterclockwise)

Preceding gene: 120609005

Following gene: 120609003

Centisome position: 6.24

GC content: 65.76

Gene sequence:

>768_bases
ATGAAGATTCTCAAGACGATGGCCACCGCCGCCGTGGCGCTGTGCGCAGCCTTCTCGGCACAGGCCCGCCCGATCGACGC
CATCCAGAAGGACGGCAAGATCATCATCGCCACGGAGGGGCAGTTCGCGCCCTTCAACTACTTCAACGGCACGCAGCTCA
CGGGCTTCGAGGTCGAGGTCGCCAACCTGGTGGCCAAGAAGATGAATCTGAAGGTGGAGTGGAAGACGCTGGGCTTCGAC
GCCCTGCTGACCGGCCTCGGGCAGGACCGCTGGGACGTCGCCATTGCCTCGCACGGCGTGACGGAAGAGCGCTCCAAGGC
CGTCACCTTCGCCGCGCCCCACTACTGCTCCGGCGGCATGGTGATCGCCATGGACCCGGCCATCCGCACGGGCAAGGACC
TCGCCGGCAAGGTGGTCGCCGTACAGACCGGCACGAGCTACCTCGAGAACGTGAAAAAGGTGCCCGGCGTGAAGGACGTG
AAGAACTTCCCGACCGACGTGGATGCCCGCAGCGCCCTCACCTCCAAGCGCGTGGACGCCTGGGTGACCGACAAGTTCGT
CGCCAAGGAAGTCGCCGCCAAGAACCCCAAGGCCGGCCTGAAGCTCGGCGACATGCTCTTCATCGAGAAGATGGCCCCGG
CCGTGGCCAAGGGCAACACCGGCCTGGCCGACGCCTGGAACAAGGCCTTCGCCGAGATCCTGGCCGACGGCAGCTACGCC
GCCGTGTCCAAGAAGTACTTCAACGAAGACGTCCGCTGCGCCCCCTGA

Upstream 100 bases:

>100_bases
CGTGACGCCCGACACCGGGCGGCGGGTCCACCATGTCCGGCTTCGCCACACGCCAAGCCGGATTTTTTTGTACCCTGTCC
CACCCCACCAGGAACCGCAG

Downstream 100 bases:

>100_bases
TCCCCTGACCGCCGGCCGGCCTTCCGGACGCTTTCCCCATGCTCACCGCTCTTTGGCCCCGGCACTGGAGCCGCCAGCAA
CGCAGCAATGCGACGCTGGT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKILKTMATAAVALCAAFSAQARPIDAIQKDGKIIIATEGQFAPFNYFNGTQLTGFEVEVANLVAKKMNLKVEWKTLGFD
ALLTGLGQDRWDVAIASHGVTEERSKAVTFAAPHYCSGGMVIAMDPAIRTGKDLAGKVVAVQTGTSYLENVKKVPGVKDV
KNFPTDVDARSALTSKRVDAWVTDKFVAKEVAAKNPKAGLKLGDMLFIEKMAPAVAKGNTGLADAWNKAFAEILADGSYA
AVSKKYFNEDVRCAP

Sequences:

>Translated_255_residues
MKILKTMATAAVALCAAFSAQARPIDAIQKDGKIIIATEGQFAPFNYFNGTQLTGFEVEVANLVAKKMNLKVEWKTLGFD
ALLTGLGQDRWDVAIASHGVTEERSKAVTFAAPHYCSGGMVIAMDPAIRTGKDLAGKVVAVQTGTSYLENVKKVPGVKDV
KNFPTDVDARSALTSKRVDAWVTDKFVAKEVAAKNPKAGLKLGDMLFIEKMAPAVAKGNTGLADAWNKAFAEILADGSYA
AVSKKYFNEDVRCAP
>Mature_255_residues
MKILKTMATAAVALCAAFSAQARPIDAIQKDGKIIIATEGQFAPFNYFNGTQLTGFEVEVANLVAKKMNLKVEWKTLGFD
ALLTGLGQDRWDVAIASHGVTEERSKAVTFAAPHYCSGGMVIAMDPAIRTGKDLAGKVVAVQTGTSYLENVKKVPGVKDV
KNFPTDVDARSALTSKRVDAWVTDKFVAKEVAAKNPKAGLKLGDMLFIEKMAPAVAKGNTGLADAWNKAFAEILADGSYA
AVSKKYFNEDVRCAP

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=255, Percent_Identity=32.9411764705882, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=31.6205533596838, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787085, Length=221, Percent_Identity=29.4117647058824, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=28.8065843621399, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788648, Length=230, Percent_Identity=30.8695652173913, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=28.740157480315, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27266; Mature: 27266

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILKTMATAAVALCAAFSAQARPIDAIQKDGKIIIATEGQFAPFNYFNGTQLTGFEVEV
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCEECCEEHHH
ANLVAKKMNLKVEWKTLGFDALLTGLGQDRWDVAIASHGVTEERSKAVTFAAPHYCSGGM
HHHHHHHHCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCHHHCCEEEEECCCCCCCCE
VIAMDPAIRTGKDLAGKVVAVQTGTSYLENVKKVPGVKDVKNFPTDVDARSALTSKRVDA
EEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHH
WVTDKFVAKEVAAKNPKAGLKLGDMLFIEKMAPAVAKGNTGLADAWNKAFAEILADGSYA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH
AVSKKYFNEDVRCAP
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILKTMATAAVALCAAFSAQARPIDAIQKDGKIIIATEGQFAPFNYFNGTQLTGFEVEV
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCEECCEEHHH
ANLVAKKMNLKVEWKTLGFDALLTGLGQDRWDVAIASHGVTEERSKAVTFAAPHYCSGGM
HHHHHHHHCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCHHHCCEEEEECCCCCCCCE
VIAMDPAIRTGKDLAGKVVAVQTGTSYLENVKKVPGVKDVKNFPTDVDARSALTSKRVDA
EEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHH
WVTDKFVAKEVAAKNPKAGLKLGDMLFIEKMAPAVAKGNTGLADAWNKAFAEILADGSYA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH
AVSKKYFNEDVRCAP
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]