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The map label for this gene is tcyB [H]

Identifier: 120609003

GI number: 120609003

Start: 332291

End: 333073

Strand: Reverse

Name: tcyB [H]

Synonym: Aave_0300

Alternate gene names: 120609003

Gene position: 333073-332291 (Counterclockwise)

Preceding gene: 120609004

Following gene: 120609002

Centisome position: 6.22

GC content: 70.37

Gene sequence:

>783_bases
ATGCTCACCGCTCTTTGGCCCCGGCACTGGAGCCGCCAGCAACGCAGCAATGCGACGCTGGTCTCGGCCCTGGTGCTGAT
GGTGCTGGCCCTGGCCCTGCTCGGCCAGGCCCTCTCCTTCCTCCCCGACCCCATCGGCCCCAATGCGCAGGCCTTCGCCG
ACGGCGCGCGCACCACGCTCTGGCTCACGCTGGTGAGCGGGCTCTGCGGCCTCGTGCTCGGCACCGGCGCGGCCCTGGCC
CGCACCGCGCGCTGGACGCCGCTGCGCTGGGCGGCCCGCTTCTACATCTGGGTGATCCGCGGCACGCCGCTGCTGGTGCA
GATCCTGTTCGTGTATTTCGCCCTGCCGGTGCTGGTTCCGGGGCTCAACCTGCCCGACTTCGCCGCCGCCGTGGTGGCGC
TGGGCCTGAACGTGGGTGCCTACAACTCCGAGGCCATCCGCGCGGGCCTGCTGGCCGTGCCGCGCGGGCAGACCGAGGCG
GCCCGCGCCCTGGGGCTGTCGCGCACGCATGTCTTCTTCGACGTGGTGTTTCCCCAGGCCTTCAAGATCTCGCTGCCGCC
GCTGGTGAGCAATTTCGTGGCACTGCTCAAGGACTCCTCGCTCGCCTACGCGATCGGCGTGGTGGAGCTGACCAACGTGG
GCAACCGCATCCAGTCGGCCACCTTCCAGCCGATCGCGACGCTCTCCACGGTGGCCGTCACCTACCTGCTGCTGACCACG
CTGGTGACCCAGGTGTCGAACGCCATCGAGCACCGCTTCGACGTGGAGGGCCGCGCCCAATGA

Upstream 100 bases:

>100_bases
ACGGCAGCTACGCCGCCGTGTCCAAGAAGTACTTCAACGAAGACGTCCGCTGCGCCCCCTGATCCCCTGACCGCCGGCCG
GCCTTCCGGACGCTTTCCCC

Downstream 100 bases:

>100_bases
CGCATCCCCACGCCACGCCCCCCACGCCCTACATCGTGGCGGAGAACGTCTGCAAGGCGTTCGGCACGCACCAGGTGCTC
AAGGATGTCTCCACCGTGTT

Product: polar amino acid ABC transporter inner membrane subunit

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLTALWPRHWSRQQRSNATLVSALVLMVLALALLGQALSFLPDPIGPNAQAFADGARTTLWLTLVSGLCGLVLGTGAALA
RTARWTPLRWAARFYIWVIRGTPLLVQILFVYFALPVLVPGLNLPDFAAAVVALGLNVGAYNSEAIRAGLLAVPRGQTEA
ARALGLSRTHVFFDVVFPQAFKISLPPLVSNFVALLKDSSLAYAIGVVELTNVGNRIQSATFQPIATLSTVAVTYLLLTT
LVTQVSNAIEHRFDVEGRAQ

Sequences:

>Translated_260_residues
MLTALWPRHWSRQQRSNATLVSALVLMVLALALLGQALSFLPDPIGPNAQAFADGARTTLWLTLVSGLCGLVLGTGAALA
RTARWTPLRWAARFYIWVIRGTPLLVQILFVYFALPVLVPGLNLPDFAAAVVALGLNVGAYNSEAIRAGLLAVPRGQTEA
ARALGLSRTHVFFDVVFPQAFKISLPPLVSNFVALLKDSSLAYAIGVVELTNVGNRIQSATFQPIATLSTVAVTYLLLTT
LVTQVSNAIEHRFDVEGRAQ
>Mature_260_residues
MLTALWPRHWSRQQRSNATLVSALVLMVLALALLGQALSFLPDPIGPNAQAFADGARTTLWLTLVSGLCGLVLGTGAALA
RTARWTPLRWAARFYIWVIRGTPLLVQILFVYFALPVLVPGLNLPDFAAAVVALGLNVGAYNSEAIRAGLLAVPRGQTEA
ARALGLSRTHVFFDVVFPQAFKISLPPLVSNFVALLKDSSLAYAIGVVELTNVGNRIQSATFQPIATLSTVAVTYLLLTT
LVTQVSNAIEHRFDVEGRAQ

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=218, Percent_Identity=38.0733944954128, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788226, Length=205, Percent_Identity=35.1219512195122, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787087, Length=222, Percent_Identity=31.0810810810811, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788645, Length=206, Percent_Identity=30.0970873786408, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786873, Length=182, Percent_Identity=32.4175824175824, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI48994930, Length=172, Percent_Identity=34.8837209302326, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786874, Length=216, Percent_Identity=25, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788646, Length=214, Percent_Identity=28.5046728971963, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787086, Length=208, Percent_Identity=30.2884615384615, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082239, Length=184, Percent_Identity=32.0652173913043, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27943; Mature: 27943

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTALWPRHWSRQQRSNATLVSALVLMVLALALLGQALSFLPDPIGPNAQAFADGARTTL
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHH
WLTLVSGLCGLVLGTGAALARTARWTPLRWAARFYIWVIRGTPLLVQILFVYFALPVLVP
HHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GLNLPDFAAAVVALGLNVGAYNSEAIRAGLLAVPRGQTEAARALGLSRTHVFFDVVFPQA
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHEEECCCCH
FKISLPPLVSNFVALLKDSSLAYAIGVVELTNVGNRIQSATFQPIATLSTVAVTYLLLTT
HEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LVTQVSNAIEHRFDVEGRAQ
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLTALWPRHWSRQQRSNATLVSALVLMVLALALLGQALSFLPDPIGPNAQAFADGARTTL
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHH
WLTLVSGLCGLVLGTGAALARTARWTPLRWAARFYIWVIRGTPLLVQILFVYFALPVLVP
HHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GLNLPDFAAAVVALGLNVGAYNSEAIRAGLLAVPRGQTEAARALGLSRTHVFFDVVFPQA
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHEEECCCCH
FKISLPPLVSNFVALLKDSSLAYAIGVVELTNVGNRIQSATFQPIATLSTVAVTYLLLTT
HEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LVTQVSNAIEHRFDVEGRAQ
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]