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The map label for this gene is zraR [H]

Identifier: 120608880

GI number: 120608880

Start: 191310

End: 192686

Strand: Reverse

Name: zraR [H]

Synonym: Aave_0172

Alternate gene names: 120608880

Gene position: 192686-191310 (Counterclockwise)

Preceding gene: 120608881

Following gene: 120608879

Centisome position: 3.6

GC content: 75.74

Gene sequence:

>1377_bases
ATGGCACGCATCCTAGCCGTCGATGACGACGCGGCCTTCCGCGAATCGCTGGTCGAAACCCTGCAGGGCCTGGGGCACGA
GGTGCACGAGGCCGCCAGCGGCGACGAGGCGCTGCGGCAGGCCGGGCGCCACCGCTACGCCGCCATCTTCCTGGACCACC
GCATGCCCGGCATGGACGGGCTGGAGACCCTCGCCGCGCTCGCCGCCCGCGTGCCCGAGCTGCCCCCGGTGGTGGTGCTC
ACCGCCTACGCCAGCGGCTCCGGCACCATCGAGGCGATGCGCCTGGGTGCCTTCGACCACCTGACCAAGCCGGTGGGCCG
CGCCGCGGTGATCGAGGTGCTGGAGCGCGCCCTGCGCTCCCGCGCCGAAGCGGACGCCGCCCCGCCCGCCCCCGCGGCGC
GTGCCGGCGCGGACGAGGCGGACGGCGAACTCATCGGCGTGAGCGAGGCCATGCGCGAGGTCCACAAGCGCATCGGGCTG
GCCGCCGCGAGCGAAGCGCCCGTGCTGGTGCAGGGCGAGACCGGCACGGGCAAGGAGCTGGTGGCGCGCGCGCTCCACCG
GCACTCGGCCCGCAGCGCCGGCCCGTTCGTGGCGCTCAATTGCGCGGCCATCCCGAAGGAGCTGCTGGAAAGCGAGCTGT
TCGGCCACGTGAAGGGCGCCTTCACCGGCGCCGCCACCGACCGGCCCGGGTGCTTCCGCGCGGCCGATGGCGGCGTGCTG
CTGCTCGACGAGATCGGCGACATGGCGCCGGACGTGCAGGCCAAGCTGCTGCGCGCCCTGCAGGAAGGCGAGGTCACGCC
CCTGGGCAGCCACCGGCCGGTGAAGGTGGACGTGCGCGTGGTGGCCGCCACGCACCGCGACCTGACCGCCGCCGTGCGCG
AGGGGCGCTTCCGCGAGGACCTGCGCTACCGGCTCGACGTGCTGCAGATCGCCCTGCCCCCGCTGCGCGAGCGGCTCGCC
GACATCGTGCCGCTGGCCGAGCATTTCCTGCGCCGTGCCGCGGCGCCGCAGCCGGCCAAGCGCCTCGCGCCGGACGCGGC
GCGCGCCCTGCTCGCGCACGGCTGGCCGGGCAACGTGCGCGAACTGCGCAACGCCATGGAGCGCGTGCAGGCCCTGCAGC
GCGGCCCCGTGGTGGGCGCGGCCGACCTCGACCTGGGCCCGCCCCCGGCATCGGCGGCGGCGTCCGGCACCGACGACGGC
GCCCTGCCGCAGGACTGGCTCGACGTGCCGCTGCCCGAGGCCGTGGAGCGGCTGGAGCGGCAGTTGATCGCCCACGCGCT
CGAACAGGCCCGCGGCAATCGTTCGCTCGCCGCCCGGCGGCTGGGCATCCACCGCCAGCTGCTCTACAGCAAGCTGGCCC
AGTACGGCATGGAATGA

Upstream 100 bases:

>100_bases
TGGGCCTGGCCCTGGCGCGCGAGGTGGCGCTGGCGCACGGGGGCGACCTGCACTACGCGCCGCGCGAAGGCGGCGGCGCC
CGCTTCCTCCTGGAGCTGCC

Downstream 100 bases:

>100_bases
AACGGATGGAGGCACCGGCGATGTCGCGAATCCGGACACAGTGTCGTCGCACCATCCATGCATTGACGATGGCCAGCAAC
GCAAGTCATTGATTCATAAG

Product: Fis family two component sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MARILAVDDDAAFRESLVETLQGLGHEVHEAASGDEALRQAGRHRYAAIFLDHRMPGMDGLETLAALAARVPELPPVVVL
TAYASGSGTIEAMRLGAFDHLTKPVGRAAVIEVLERALRSRAEADAAPPAPAARAGADEADGELIGVSEAMREVHKRIGL
AAASEAPVLVQGETGTGKELVARALHRHSARSAGPFVALNCAAIPKELLESELFGHVKGAFTGAATDRPGCFRAADGGVL
LLDEIGDMAPDVQAKLLRALQEGEVTPLGSHRPVKVDVRVVAATHRDLTAAVREGRFREDLRYRLDVLQIALPPLRERLA
DIVPLAEHFLRRAAAPQPAKRLAPDAARALLAHGWPGNVRELRNAMERVQALQRGPVVGAADLDLGPPPASAAASGTDDG
ALPQDWLDVPLPEAVERLERQLIAHALEQARGNRSLAARRLGIHRQLLYSKLAQYGME

Sequences:

>Translated_458_residues
MARILAVDDDAAFRESLVETLQGLGHEVHEAASGDEALRQAGRHRYAAIFLDHRMPGMDGLETLAALAARVPELPPVVVL
TAYASGSGTIEAMRLGAFDHLTKPVGRAAVIEVLERALRSRAEADAAPPAPAARAGADEADGELIGVSEAMREVHKRIGL
AAASEAPVLVQGETGTGKELVARALHRHSARSAGPFVALNCAAIPKELLESELFGHVKGAFTGAATDRPGCFRAADGGVL
LLDEIGDMAPDVQAKLLRALQEGEVTPLGSHRPVKVDVRVVAATHRDLTAAVREGRFREDLRYRLDVLQIALPPLRERLA
DIVPLAEHFLRRAAAPQPAKRLAPDAARALLAHGWPGNVRELRNAMERVQALQRGPVVGAADLDLGPPPASAAASGTDDG
ALPQDWLDVPLPEAVERLERQLIAHALEQARGNRSLAARRLGIHRQLLYSKLAQYGME
>Mature_457_residues
ARILAVDDDAAFRESLVETLQGLGHEVHEAASGDEALRQAGRHRYAAIFLDHRMPGMDGLETLAALAARVPELPPVVVLT
AYASGSGTIEAMRLGAFDHLTKPVGRAAVIEVLERALRSRAEADAAPPAPAARAGADEADGELIGVSEAMREVHKRIGLA
AASEAPVLVQGETGTGKELVARALHRHSARSAGPFVALNCAAIPKELLESELFGHVKGAFTGAATDRPGCFRAADGGVLL
LDEIGDMAPDVQAKLLRALQEGEVTPLGSHRPVKVDVRVVAATHRDLTAAVREGRFREDLRYRLDVLQIALPPLRERLAD
IVPLAEHFLRRAAAPQPAKRLAPDAARALLAHGWPGNVRELRNAMERVQALQRGPVVGAADLDLGPPPASAAASGTDDGA
LPQDWLDVPLPEAVERLERQLIAHALEQARGNRSLAARRLGIHRQLLYSKLAQYGME

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=462, Percent_Identity=41.991341991342, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1790437, Length=453, Percent_Identity=45.6953642384106, Blast_Score=341, Evalue=6e-95,
Organism=Escherichia coli, GI1790299, Length=471, Percent_Identity=43.7367303609342, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI1788905, Length=459, Percent_Identity=40.3050108932462, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1789233, Length=323, Percent_Identity=44.5820433436532, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082117, Length=324, Percent_Identity=44.7530864197531, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=43.1137724550898, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1786524, Length=332, Percent_Identity=44.578313253012, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082152, Length=312, Percent_Identity=44.8717948717949, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1787583, Length=320, Percent_Identity=40.9375, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87081872, Length=316, Percent_Identity=41.4556962025316, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789828, Length=220, Percent_Identity=42.7272727272727, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87081858, Length=299, Percent_Identity=32.1070234113712, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48855; Mature: 48724

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARILAVDDDAAFRESLVETLQGLGHEVHEAASGDEALRQAGRHRYAAIFLDHRMPGMDG
CCEEEEECCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCEEEEEEEECCCCCCCH
LETLAALAARVPELPPVVVLTAYASGSGTIEAMRLGAFDHLTKPVGRAAVIEVLERALRS
HHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEADAAPPAPAARAGADEADGELIGVSEAMREVHKRIGLAAASEAPVLVQGETGTGKEL
HHHCCCCCCCCCHHCCCCCCCCCEECHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHH
VARALHRHSARSAGPFVALNCAAIPKELLESELFGHVKGAFTGAATDRPGCFRAADGGVL
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEE
LLDEIGDMAPDVQAKLLRALQEGEVTPLGSHRPVKVDVRVVAATHRDLTAAVREGRFRED
EHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHCCCHHHH
LRYRLDVLQIALPPLRERLADIVPLAEHFLRRAAAPQPAKRLAPDAARALLAHGWPGNVR
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCCCCHH
ELRNAMERVQALQRGPVVGAADLDLGPPPASAAASGTDDGALPQDWLDVPLPEAVERLER
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHH
QLIAHALEQARGNRSLAARRLGIHRQLLYSKLAQYGME
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARILAVDDDAAFRESLVETLQGLGHEVHEAASGDEALRQAGRHRYAAIFLDHRMPGMDG
CEEEEECCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCEEEEEEEECCCCCCCH
LETLAALAARVPELPPVVVLTAYASGSGTIEAMRLGAFDHLTKPVGRAAVIEVLERALRS
HHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEADAAPPAPAARAGADEADGELIGVSEAMREVHKRIGLAAASEAPVLVQGETGTGKEL
HHHCCCCCCCCCHHCCCCCCCCCEECHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHH
VARALHRHSARSAGPFVALNCAAIPKELLESELFGHVKGAFTGAATDRPGCFRAADGGVL
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEE
LLDEIGDMAPDVQAKLLRALQEGEVTPLGSHRPVKVDVRVVAATHRDLTAAVREGRFRED
EHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHCCCHHHH
LRYRLDVLQIALPPLRERLADIVPLAEHFLRRAAAPQPAKRLAPDAARALLAHGWPGNVR
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCCCCHH
ELRNAMERVQALQRGPVVGAADLDLGPPPASAAASGTDDGALPQDWLDVPLPEAVERLER
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHH
QLIAHALEQARGNRSLAARRLGIHRQLLYSKLAQYGME
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]