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The map label for this gene is purN

Identifier: 120599231

GI number: 120599231

Start: 2800068

End: 2800712

Strand: Direct

Name: purN

Synonym: Sputw3181_2427

Alternate gene names: 120599231

Gene position: 2800068-2800712 (Clockwise)

Preceding gene: 120599230

Following gene: 120599232

Centisome position: 59.47

GC content: 48.37

Gene sequence:

>645_bases
ATGCCCCAGCGCTGTCGTGTAGTAGTCTTAATTTCCGGAAATGGCAGTAACCTGCAAGCGATTATCGACGGTTGTGACGA
TAATCTGCAGGCCGAAGTTGTCGGAGTGATCAGTAATAAACCCGATGCCTATGGCCTAGTTCGCGCCCATTATAATGAAA
TTGATACTAGCTGCGTGATAGCGCATCAAGGTGAATCTCGCTCTGAATACGATGCACGCTTAATCGCGGTGATTGAACAG
TATCAACCCGATTTAATCGTATTAGCGGGATTTATGCGGATCTTAACCGATGACCTCGTCAATCGTTATTTAGGCCGGAT
AATCAACATCCATCCTTCGCTGTTACCCAAATACACTGGCTTAAACACTCATCAACGCGCCATCGACGCTAACGACAGCG
AACATGGTGCTAGCGTGCATTTTGTCACGCCTGAGCTGGATGCAGGCCCTGTGATATTACAGGCCAAAGTCCCTGTTTAT
GAAGACGATACGGCGGACATGCTTGCCGCTCGCGTACATGAGCAAGAACATGCGATTTATCCACTCGTGGTGAAATGGTT
TAGTCAGCAACGTCTTAACATGCAAAACGGCCAAGCCTATCTCGATGGCAACCTTATCGGCCCAAGCGGTTATGCGCCTG
ATTAG

Upstream 100 bases:

>100_bases
ATGCAGCACTTGCATTACTGGCAGCAGAAGGCGAACAAGCATGGCTTATCGGTGCTATTGCACATCGAGAAGGCAATGAA
GAGCAAGTGGAGATCCTGTA

Downstream 100 bases:

>100_bases
TTCCACTTGACAGTCAGTCGTATCAATCAAAAATAGAGACCTGCGGGTCTCTATTTTTTTAACTATTTTCTACAAAAGCA
TTGTTTTTTAGGACAATGTG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPQRCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHYNEIDTSCVIAHQGESRSEYDARLIAVIEQ
YQPDLIVLAGFMRILTDDLVNRYLGRIINIHPSLLPKYTGLNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVY
EDDTADMLAARVHEQEHAIYPLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD

Sequences:

>Translated_214_residues
MPQRCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHYNEIDTSCVIAHQGESRSEYDARLIAVIEQ
YQPDLIVLAGFMRILTDDLVNRYLGRIINIHPSLLPKYTGLNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVY
EDDTADMLAARVHEQEHAIYPLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD
>Mature_213_residues
PQRCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHYNEIDTSCVIAHQGESRSEYDARLIAVIEQY
QPDLIVLAGFMRILTDDLVNRYLGRIINIHPSLLPKYTGLNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVYE
DDTADMLAARVHEQEHAIYPLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=40.4040404040404, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=40.4040404040404, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=40.4040404040404, Blast_Score=152, Evalue=2e-37,
Organism=Escherichia coli, GI1788846, Length=209, Percent_Identity=58.8516746411483, Blast_Score=247, Evalue=4e-67,
Organism=Escherichia coli, GI1787483, Length=190, Percent_Identity=31.0526315789474, Blast_Score=94, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=182, Percent_Identity=37.3626373626374, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320616, Length=197, Percent_Identity=32.994923857868, Blast_Score=88, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24582400, Length=183, Percent_Identity=49.1803278688525, Blast_Score=163, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23632; Mature: 23501

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQRCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHYNEIDTSCVI
CCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEE
AHQGESRSEYDARLIAVIEQYQPDLIVLAGFMRILTDDLVNRYLGRIINIHPSLLPKYTG
EECCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCC
LNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVYEDDTADMLAARVHEQEHAIY
CCCHHHCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEECCHHHHHHHHHHHHCCCHH
PLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD
HHHHHHHHHHHCCCCCCCEEECCEEECCCCCCCC
>Mature Secondary Structure 
PQRCRVVVLISGNGSNLQAIIDGCDDNLQAEVVGVISNKPDAYGLVRAHYNEIDTSCVI
CCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEE
AHQGESRSEYDARLIAVIEQYQPDLIVLAGFMRILTDDLVNRYLGRIINIHPSLLPKYTG
EECCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCC
LNTHQRAIDANDSEHGASVHFVTPELDAGPVILQAKVPVYEDDTADMLAARVHEQEHAIY
CCCHHHCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEECCHHHHHHHHHHHHCCCHH
PLVVKWFSQQRLNMQNGQAYLDGNLIGPSGYAPD
HHHHHHHHHHHCCCCCCCEEECCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]