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The map label for this gene is cpdB

Identifier: 120599215

GI number: 120599215

Start: 2781818

End: 2783884

Strand: Direct

Name: cpdB

Synonym: Sputw3181_2411

Alternate gene names: 120599215

Gene position: 2781818-2783884 (Clockwise)

Preceding gene: 120599208

Following gene: 120599216

Centisome position: 59.08

GC content: 43.98

Gene sequence:

>2067_bases
ATGTTAAATAAGAAAATACTTGCAGTGATGATCACTGCCGCCCTTGGCGTGAGTGCCTGTGGCTCAGATGATGACACGAT
TATTCAACCTGATGACCGTGTCGTTCAAGCAGATTTACGGGTGCTAGAAACCACAGATTTGCACACAAATATCATGGATT
TCAATTACTACAGTGGTAAAAATGATCCAACTATCGGTCTAGCTCGCACTGCAAGCTTAATTCACGCTGCACGCAGTGAA
GCCAAAAACAGTGTACTCGTTGATAATGGCGATCTGCTACAAGGCAGCCCAATGGGCGATTATATGGCTAAAGTTGGCCT
GCAAATGGGTGATATTCATCCAGCCTATAAAGCCATGAACCTATTAGATTATGAAGTCGGTAATATCGGTAACCATGAAT
TTAACTATGGTTTAGATTTCTTAAATAAATCCATTAGTGGTGCAGATTTCCCTTATATCAACTCAAACGTTTATTGCGCC
GACGATAATGGTTGCTGGAATGAGGTTAAAAAAGGTGAGCATTTATTCACCCCTTATCTCATCAAACAAAAAAATATTAT
CGACAGTGAAGGTAATAATCAAACCATTAACATTGGTTATATTGGTTTTGTGCCACCGCAAATTCTATTGTGGGATAAAC
AAAATCTGACGGGTAAAGTCACGGTCGAAGATATTATTGAAACCGCGAAAAAATATGTCCCCGAGATGAAAGCAAAAGGT
GCCGATCTTATTATCGCCATCCCTCATTCTGGTATTGGATCAACTGAAAATCCTGGCGATCCTATGGCTGAAAACGCCAC
TTATGCTTTGACCTATGTAAACGGCATAGATGCGATTCTCTTTGGTCACAGCCATTCCATCTTCCCAGATGCTAAGTATG
CTGACTTACCTAATACCGATGTCACTAAAGGCTTACTGAACGGCATACCTGCAGTTATGCCAGGACGTTGGGGTGATAAC
TTGGGAGTGGTTGACTTTAAACTTGAGCGTAAAGATGGTAAATGGCATGTCCTTAGTGCCAAAACGCAAGCTCGCCCTAT
CTATAATGGTACAACTAAGGAAGAGTTAGTTAGCCAAGATCCTGCAATTAGAGATGCAGTCGCAGCAGAACACCAAGGCA
CCCTCGCTTTTGTGGATGAACCTATCGGCGTTGCCGCAGCGGACATGTACAGCTTCCTGACCTTAGTGCAGGACGATCCA
ACGGTACAAATCGTGTCCGATGCACAAATCGCTAACGTGAAAGCAAAATTACCTCCGGAATTAAAAGATCTGCCAGTGCT
ATCGGCCTCAGCTCCTTTTAAAGCGGGTGGTCGCCACGGTACAACCAGCGATGCCGATCAATTTGTGCAAGTTGATAAAG
GCCCACTGACCTTTAAAAACGCCGCCGATCTGTACCTATATCCCAATACTATGGTGGCAGTCAAAGCGACAGGTGCAGAG
TTAAAAGATTGGCTCGAGTGCTCAGCGAACCAATTCAATCAAATCGATCCAACAAAAACCACACCACAAAGTTTAATTAA
CTGGGATGGTCATCCAACCTATAACTTCGACGTACTCGATGGCTTAACGTACAAGATAGATGTAACTCAACCGAGCAAGT
TTGACCGCGATTGCGCCGTCGTTAATGCCAACGCAAACCGTATCGTTGATTTAAGCTATAAAGACGAAAACGGCAAAGTC
ATCACAGGTGAAGAATTTGCCGCGAAAGCGTTTATTGTCGCCTCAAATAACTACCGTGCCTTTGGCGGTAAGTTTGCAGG
TACGGGTAGCGACCATGTGGTGCTTGAACTACCTGACACTAACCGTGAAGCGTTAGCGGCCTATATCACAGCGCAATCTC
AGTACAACGAAACCACTGGCAAATACGAAGCTATGGTAAATCCAAGTGCCGATTACAACTGGGATTTCAAAACCATCAGC
ACTAACGTCACCTTAGATGTTCGCTTCGAAACCCAAGACAGTGACAAAGCCGCAGCCTTTATCGAAGCTAACAAGCAACG
TGAAATGAAGAAGATCAGCAAGGATGAACTGGGTTTCGCCGTTTATAGCATCAACCTAACCAAATAA

Upstream 100 bases:

>100_bases
AATTAACCTAACAGTTAAATTACGCTGAATTAACAGGATGATGAGCATGCTCCTTTACGGAGCATGCATTGGGGTCAAAT
AAATATAAATAGAGATAACT

Downstream 100 bases:

>100_bases
GCGTTCAATCCCGAAATATCATGCTCAAAAAGGCTCCTCGTGAGCCTTTTTTACTCTCAATGACAAAACCAACCTTAATG
GACGGCTCCTATAAATGGTT

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MLNKKILAVMITAALGVSACGSDDDTIIQPDDRVVQADLRVLETTDLHTNIMDFNYYSGKNDPTIGLARTASLIHAARSE
AKNSVLVDNGDLLQGSPMGDYMAKVGLQMGDIHPAYKAMNLLDYEVGNIGNHEFNYGLDFLNKSISGADFPYINSNVYCA
DDNGCWNEVKKGEHLFTPYLIKQKNIIDSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIIETAKKYVPEMKAKG
ADLIIAIPHSGIGSTENPGDPMAENATYALTYVNGIDAILFGHSHSIFPDAKYADLPNTDVTKGLLNGIPAVMPGRWGDN
LGVVDFKLERKDGKWHVLSAKTQARPIYNGTTKEELVSQDPAIRDAVAAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDP
TVQIVSDAQIANVKAKLPPELKDLPVLSASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAE
LKDWLECSANQFNQIDPTKTTPQSLINWDGHPTYNFDVLDGLTYKIDVTQPSKFDRDCAVVNANANRIVDLSYKDENGKV
ITGEEFAAKAFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAAYITAQSQYNETTGKYEAMVNPSADYNWDFKTIS
TNVTLDVRFETQDSDKAAAFIEANKQREMKKISKDELGFAVYSINLTK

Sequences:

>Translated_688_residues
MLNKKILAVMITAALGVSACGSDDDTIIQPDDRVVQADLRVLETTDLHTNIMDFNYYSGKNDPTIGLARTASLIHAARSE
AKNSVLVDNGDLLQGSPMGDYMAKVGLQMGDIHPAYKAMNLLDYEVGNIGNHEFNYGLDFLNKSISGADFPYINSNVYCA
DDNGCWNEVKKGEHLFTPYLIKQKNIIDSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIIETAKKYVPEMKAKG
ADLIIAIPHSGIGSTENPGDPMAENATYALTYVNGIDAILFGHSHSIFPDAKYADLPNTDVTKGLLNGIPAVMPGRWGDN
LGVVDFKLERKDGKWHVLSAKTQARPIYNGTTKEELVSQDPAIRDAVAAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDP
TVQIVSDAQIANVKAKLPPELKDLPVLSASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAE
LKDWLECSANQFNQIDPTKTTPQSLINWDGHPTYNFDVLDGLTYKIDVTQPSKFDRDCAVVNANANRIVDLSYKDENGKV
ITGEEFAAKAFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAAYITAQSQYNETTGKYEAMVNPSADYNWDFKTIS
TNVTLDVRFETQDSDKAAAFIEANKQREMKKISKDELGFAVYSINLTK
>Mature_688_residues
MLNKKILAVMITAALGVSACGSDDDTIIQPDDRVVQADLRVLETTDLHTNIMDFNYYSGKNDPTIGLARTASLIHAARSE
AKNSVLVDNGDLLQGSPMGDYMAKVGLQMGDIHPAYKAMNLLDYEVGNIGNHEFNYGLDFLNKSISGADFPYINSNVYCA
DDNGCWNEVKKGEHLFTPYLIKQKNIIDSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIIETAKKYVPEMKAKG
ADLIIAIPHSGIGSTENPGDPMAENATYALTYVNGIDAILFGHSHSIFPDAKYADLPNTDVTKGLLNGIPAVMPGRWGDN
LGVVDFKLERKDGKWHVLSAKTQARPIYNGTTKEELVSQDPAIRDAVAAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDP
TVQIVSDAQIANVKAKLPPELKDLPVLSASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAE
LKDWLECSANQFNQIDPTKTTPQSLINWDGHPTYNFDVLDGLTYKIDVTQPSKFDRDCAVVNANANRIVDLSYKDENGKV
ITGEEFAAKAFIVASNNYRAFGGKFAGTGSDHVVLELPDTNREALAAYITAQSQYNETTGKYEAMVNPSADYNWDFKTIS
TNVTLDVRFETQDSDKAAAFIEANKQREMKKISKDELGFAVYSINLTK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=665, Percent_Identity=53.8345864661654, Blast_Score=743, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=535, Percent_Identity=22.9906542056075, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 75209; Mature: 75209

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKKILAVMITAALGVSACGSDDDTIIQPDDRVVQADLRVLETTDLHTNIMDFNYYSGK
CCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEECEEEEEECCCCCEEEEEEEECCC
NDPTIGLARTASLIHAARSEAKNSVLVDNGDLLQGSPMGDYMAKVGLQMGDIHPAYKAMN
CCCEEEHHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHCCCCCCCCHHHHHHH
LLDYEVGNIGNHEFNYGLDFLNKSISGADFPYINSNVYCADDNGCWNEVKKGEHLFTPYL
HHEEECCCCCCCCCCCCHHHHHCCCCCCCCCEECCEEEEECCCCCHHHHCCCCEEECCEE
IKQKNIIDSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIIETAKKYVPEMKAKG
EEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHHHHCCHHHCCC
ADLIIAIPHSGIGSTENPGDPMAENATYALTYVNGIDAILFGHSHSIFPDAKYADLPNTD
CCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCH
VTKGLLNGIPAVMPGRWGDNLGVVDFKLERKDGKWHVLSAKTQARPIYNGTTKEELVSQD
HHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHCC
PAIRDAVAAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDPTVQIVSDAQIANVKAKLPPE
CCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCC
LKDLPVLSASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAE
HHHCCEEECCCCCCCCCCCCCCCCCHHEEEECCCCEEECCCCEEEEECCEEEEEEECCHH
LKDWLECSANQFNQIDPTKTTPQSLINWDGHPTYNFDVLDGLTYKIDVTQPSKFDRDCAV
HHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCEEEEEEECCCCCCCCCEEE
VNANANRIVDLSYKDENGKVITGEEFAAKAFIVASNNYRAFGGKFAGTGSDHVVLELPDT
EECCCCEEEEEEEECCCCCEEECCCCCEEEEEEECCCEEECCCEECCCCCCEEEEECCCC
NREALAAYITAQSQYNETTGKYEAMVNPSADYNWDFKTISTNVTLDVRFETQDSDKAAAF
CHHHEEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEEEEEEEECCCCCCEEEE
IEANKQREMKKISKDELGFAVYSINLTK
EECHHHHHHHHCCHHHCCEEEEEEEECC
>Mature Secondary Structure
MLNKKILAVMITAALGVSACGSDDDTIIQPDDRVVQADLRVLETTDLHTNIMDFNYYSGK
CCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEECEEEEEECCCCCEEEEEEEECCC
NDPTIGLARTASLIHAARSEAKNSVLVDNGDLLQGSPMGDYMAKVGLQMGDIHPAYKAMN
CCCEEEHHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHCCCCCCCCHHHHHHH
LLDYEVGNIGNHEFNYGLDFLNKSISGADFPYINSNVYCADDNGCWNEVKKGEHLFTPYL
HHEEECCCCCCCCCCCCHHHHHCCCCCCCCCEECCEEEEECCCCCHHHHCCCCEEECCEE
IKQKNIIDSEGNNQTINIGYIGFVPPQILLWDKQNLTGKVTVEDIIETAKKYVPEMKAKG
EEECCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHHHHCCHHHCCC
ADLIIAIPHSGIGSTENPGDPMAENATYALTYVNGIDAILFGHSHSIFPDAKYADLPNTD
CCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCH
VTKGLLNGIPAVMPGRWGDNLGVVDFKLERKDGKWHVLSAKTQARPIYNGTTKEELVSQD
HHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHCC
PAIRDAVAAEHQGTLAFVDEPIGVAAADMYSFLTLVQDDPTVQIVSDAQIANVKAKLPPE
CCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCC
LKDLPVLSASAPFKAGGRHGTTSDADQFVQVDKGPLTFKNAADLYLYPNTMVAVKATGAE
HHHCCEEECCCCCCCCCCCCCCCCCHHEEEECCCCEEECCCCEEEEECCEEEEEEECCHH
LKDWLECSANQFNQIDPTKTTPQSLINWDGHPTYNFDVLDGLTYKIDVTQPSKFDRDCAV
HHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCEEEEEEECCCCCCCCCEEE
VNANANRIVDLSYKDENGKVITGEEFAAKAFIVASNNYRAFGGKFAGTGSDHVVLELPDT
EECCCCEEEEEEEECCCCCEEECCCCCEEEEEEECCCEEECCCEECCCCCCEEEEECCCC
NREALAAYITAQSQYNETTGKYEAMVNPSADYNWDFKTISTNVTLDVRFETQDSDKAAAF
CHHHEEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEEEEEEEECCCCCCEEEE
IEANKQREMKKISKDELGFAVYSINLTK
EECHHHHHHHHCCHHHCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA