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The map label for this gene is ddl

Identifier: 120599029

GI number: 120599029

Start: 2572278

End: 2573291

Strand: Direct

Name: ddl

Synonym: Sputw3181_2224

Alternate gene names: 120599029

Gene position: 2572278-2573291 (Clockwise)

Preceding gene: 120599027

Following gene: 120599035

Centisome position: 54.63

GC content: 43.98

Gene sequence:

>1014_bases
ATGTCTAGAATTAACTTACTTTTGCTGTGCGGCGGCGGAAGTGCTGAACACGATATTTCACTAATGTCGGCTAACTATTT
TGAATCCTCATTGGCAAAATCCGATCAATTTTCCGTGTTACGGGTAGAACTTGATAAATTGGGTCAATATCGCACCGCGG
CAGGCGATGAGTGTGAACTGACGAATAATCGCGAAATCCGTTTTCGCGATGAAACTAAAGCGCCTTGGCCTGTGGACTAT
GTTATTCCTTGCATCCATGGTTATCCCGGCGAAACAGGTGATATCCAGTCCTATTTCAACCTTATCCAACTGCCCTACTT
CGGCTGTGAATCCGAAGCCAGTAGTAATTGTTTTAATAAAATCACCGCCAAAATGTGGTTTTCAGCCCTAGGGATCCCCA
ATACTCCCTACATTTTTCTACACCAATATGATGATGAGGCGATCAGCCAAGCACAAACTGCCTTGGCTAATTGGGGTTCA
ATCTTTATTAAAGCCGCGTCGCAAGGATCGTCTGTCGGTTGCTATAAAGTGGATGACAGCAGCAAAGTGGCACAAGTACT
CAAAGATGCCTTCGGCTACGCACCCTATGTTGTCGTGGAAAAAACCATTAAAGCCCGTGAATTAGAAGTCGCGGTATACG
AATACAATGGTGAGATTGTGGCAACCCTGCCAGGTGAAATTATCTGTGATACCAATACCTTCTACACCTTTGATGAAAAA
TACGCCAAAAGCAGTAAGGCACGCACCGATGTGGTTGCAAAAGATGTTTCTGTAGAACTCAGTGAACAGATCCGTGCTTA
TGCTATCAAGGCATTTAAGGGGATGAAGCTGCGTCACCTGTCACGTATCGACTTTTTCTTAACCGCTGAAAATGAAATTT
TGCTCAACGAAATCAACACATTCCCTGGTTCGACACCGATTTCAATGTTCCCTAAAATGTTGCAAAACCACGGCCACGAC
TTTACCGAATATTTAAGCCTAGTGATCAACAACCAGTTATCGGCAAAGGATTAA

Upstream 100 bases:

>100_bases
CACTCATAGGGATAACAAAACTAAGATGGCTACGGCTATTTTATAGGATGATTGACCAACACATGGCTCTCATCTGATCT
TGCTCAATTGAGGAATGACA

Downstream 100 bases:

>100_bases
ATCCGTTTTATTGTAGGTTATGTGAAAGAGCGCCAATGGCGCTCTTTTTGATGTTGTCATGCAGTTTAGTTACACATAAA
TGCGAACACACTAATGTGGC

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSRINLLLLCGGGSAEHDISLMSANYFESSLAKSDQFSVLRVELDKLGQYRTAAGDECELTNNREIRFRDETKAPWPVDY
VIPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNKITAKMWFSALGIPNTPYIFLHQYDDEAISQAQTALANWGS
IFIKAASQGSSVGCYKVDDSSKVAQVLKDAFGYAPYVVVEKTIKARELEVAVYEYNGEIVATLPGEIICDTNTFYTFDEK
YAKSSKARTDVVAKDVSVELSEQIRAYAIKAFKGMKLRHLSRIDFFLTAENEILLNEINTFPGSTPISMFPKMLQNHGHD
FTEYLSLVINNQLSAKD

Sequences:

>Translated_337_residues
MSRINLLLLCGGGSAEHDISLMSANYFESSLAKSDQFSVLRVELDKLGQYRTAAGDECELTNNREIRFRDETKAPWPVDY
VIPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNKITAKMWFSALGIPNTPYIFLHQYDDEAISQAQTALANWGS
IFIKAASQGSSVGCYKVDDSSKVAQVLKDAFGYAPYVVVEKTIKARELEVAVYEYNGEIVATLPGEIICDTNTFYTFDEK
YAKSSKARTDVVAKDVSVELSEQIRAYAIKAFKGMKLRHLSRIDFFLTAENEILLNEINTFPGSTPISMFPKMLQNHGHD
FTEYLSLVINNQLSAKD
>Mature_336_residues
SRINLLLLCGGGSAEHDISLMSANYFESSLAKSDQFSVLRVELDKLGQYRTAAGDECELTNNREIRFRDETKAPWPVDYV
IPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNKITAKMWFSALGIPNTPYIFLHQYDDEAISQAQTALANWGSI
FIKAASQGSSVGCYKVDDSSKVAQVLKDAFGYAPYVVVEKTIKARELEVAVYEYNGEIVATLPGEIICDTNTFYTFDEKY
AKSSKARTDVVAKDVSVELSEQIRAYAIKAFKGMKLRHLSRIDFFLTAENEILLNEINTFPGSTPISMFPKMLQNHGHDF
TEYLSLVINNQLSAKD

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=33.6182336182336, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786280, Length=328, Percent_Identity=26.5243902439024, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 37699; Mature: 37568

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRINLLLLCGGGSAEHDISLMSANYFESSLAKSDQFSVLRVELDKLGQYRTAAGDECEL
CCCEEEEEEECCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCEEE
TNNREIRFRDETKAPWPVDYVIPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNK
CCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
ITAKMWFSALGIPNTPYIFLHQYDDEAISQAQTALANWGSIFIKAASQGSSVGCYKVDDS
HHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCC
SKVAQVLKDAFGYAPYVVVEKTIKARELEVAVYEYNGEIVATLPGEIICDTNTFYTFDEK
HHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCEEEEECCCCEEECCCCEEEECHH
YAKSSKARTDVVAKDVSVELSEQIRAYAIKAFKGMKLRHLSRIDFFLTAENEILLNEINT
HHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECCEEEEEEHHC
FPGSTPISMFPKMLQNHGHDFTEYLSLVINNQLSAKD
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SRINLLLLCGGGSAEHDISLMSANYFESSLAKSDQFSVLRVELDKLGQYRTAAGDECEL
CCEEEEEEECCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCEEE
TNNREIRFRDETKAPWPVDYVIPCIHGYPGETGDIQSYFNLIQLPYFGCESEASSNCFNK
CCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
ITAKMWFSALGIPNTPYIFLHQYDDEAISQAQTALANWGSIFIKAASQGSSVGCYKVDDS
HHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCC
SKVAQVLKDAFGYAPYVVVEKTIKARELEVAVYEYNGEIVATLPGEIICDTNTFYTFDEK
HHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEECCEEEEECCCCEEECCCCEEEECHH
YAKSSKARTDVVAKDVSVELSEQIRAYAIKAFKGMKLRHLSRIDFFLTAENEILLNEINT
HHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECCEEEEEEHHC
FPGSTPISMFPKMLQNHGHDFTEYLSLVINNQLSAKD
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA