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The map label for this gene is pyrF [H]

Identifier: 120598789

GI number: 120598789

Start: 2289222

End: 2289917

Strand: Direct

Name: pyrF [H]

Synonym: Sputw3181_1975

Alternate gene names: 120598789

Gene position: 2289222-2289917 (Clockwise)

Preceding gene: 120598788

Following gene: 120598790

Centisome position: 48.62

GC content: 46.84

Gene sequence:

>696_bases
ATGACCACTAAACCTATCCTCGTAGCACTTGATTACGATAATAAAAGCCACGCGTTGCAACTCATAGATCAACTCGATCC
CAACATGTGCCGCCTTAAAATTGGCAAAGAAATGTTTACCCTATTTGGGCCTCAGCTCGTTAAGGATATCCATGATCGTG
GCTTCGATTTATTCCTAGATTTAAAATTCCACGACATTCCAAATACAGTCGCTAAAGCCGTGGCCGCTGCCGCTGATCTA
GGGGTATGGATGACGAACGTGCATGCCAGTGGTGGTTTAGCCATGATGGAAGCGGCAAAAAAAGCGTTGTTGCCATACGG
TGACAAAGCGCCAATGCTGATTGCTGTGACGGTATTAACCTCCATGAGTGATGATGATCTTAAATTAATCGGCATCAATG
TGCCTGCCTTTGAACATGTTCAGCGTTTAGCTAAATTGACGAAACAGGCTGGTCTTGATGGTGTTGTTTGTTCAGCGCAA
GAAGCAAAGGCACTTAAAACGTTATTAGGTCAAGACTTTAAACTCATTACACCAGGCATTCGTCCTGTGGGTAGCGAGGT
TGGCGACCAACATCGGGTGATGACACCGGCTGCGGCGATAGCCGCCGGGTCAGATTATTTGGTGGTTGGCCGCCCCATTA
CTAAAGCCGTCGATCCATTAGCGGCCTTGCAAGCGATTTATCAATCATTGGCATAG

Upstream 100 bases:

>100_bases
ATCTTGTAAAAATTGGGGCAGTATAAAACGGATCAGAGGGCTTGACGGCGAATAATCTAGTAGAGAAATACACAGTTTGT
GTAACCCCGTTGGAGAGATG

Downstream 100 bases:

>100_bases
AATAATAAATCACACCTGAGTGTTTATTCTCACTGTTCACGGAGAACGCCCATGTTTAATGCTACGATGTTTAACTATCA
AGGTAAGAATGTTGTCGTTG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTTKPILVALDYDNKSHALQLIDQLDPNMCRLKIGKEMFTLFGPQLVKDIHDRGFDLFLDLKFHDIPNTVAKAVAAAADL
GVWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLTSMSDDDLKLIGINVPAFEHVQRLAKLTKQAGLDGVVCSAQ
EAKALKTLLGQDFKLITPGIRPVGSEVGDQHRVMTPAAAIAAGSDYLVVGRPITKAVDPLAALQAIYQSLA

Sequences:

>Translated_231_residues
MTTKPILVALDYDNKSHALQLIDQLDPNMCRLKIGKEMFTLFGPQLVKDIHDRGFDLFLDLKFHDIPNTVAKAVAAAADL
GVWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLTSMSDDDLKLIGINVPAFEHVQRLAKLTKQAGLDGVVCSAQ
EAKALKTLLGQDFKLITPGIRPVGSEVGDQHRVMTPAAAIAAGSDYLVVGRPITKAVDPLAALQAIYQSLA
>Mature_230_residues
TTKPILVALDYDNKSHALQLIDQLDPNMCRLKIGKEMFTLFGPQLVKDIHDRGFDLFLDLKFHDIPNTVAKAVAAAADLG
VWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLTSMSDDDLKLIGINVPAFEHVQRLAKLTKQAGLDGVVCSAQE
AKALKTLLGQDFKLITPGIRPVGSEVGDQHRVMTPAAAIAAGSDYLVVGRPITKAVDPLAALQAIYQSLA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=230, Percent_Identity=63.0434782608696, Blast_Score=303, Evalue=5e-84,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 24739; Mature: 24608

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00599 AA_TRANSFER_CLASS_2 ; PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKPILVALDYDNKSHALQLIDQLDPNMCRLKIGKEMFTLFGPQLVKDIHDRGFDLFLD
CCCCCEEEEEECCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCHHHHHHHHHCCCEEEEE
LKFHDIPNTVAKAVAAAADLGVWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLT
EEECCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHEEHHH
SMSDDDLKLIGINVPAFEHVQRLAKLTKQAGLDGVVCSAQEAKALKTLLGQDFKLITPGI
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCEEECCCC
RPVGSEVGDQHRVMTPAAAIAAGSDYLVVGRPITKAVDPLAALQAIYQSLA
CCCCHHCCCCCEEECCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTKPILVALDYDNKSHALQLIDQLDPNMCRLKIGKEMFTLFGPQLVKDIHDRGFDLFLD
CCCCEEEEEECCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCHHHHHHHHHCCCEEEEE
LKFHDIPNTVAKAVAAAADLGVWMTNVHASGGLAMMEAAKKALLPYGDKAPMLIAVTVLT
EEECCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHEEHHH
SMSDDDLKLIGINVPAFEHVQRLAKLTKQAGLDGVVCSAQEAKALKTLLGQDFKLITPGI
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCEEECCCC
RPVGSEVGDQHRVMTPAAAIAAGSDYLVVGRPITKAVDPLAALQAIYQSLA
CCCCHHCCCCCEEECCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]