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The map label for this gene is trmJ [H]

Identifier: 120598674

GI number: 120598674

Start: 2143275

End: 2144000

Strand: Direct

Name: trmJ [H]

Synonym: Sputw3181_1859

Alternate gene names: 120598674

Gene position: 2143275-2144000 (Clockwise)

Preceding gene: 120598672

Following gene: 120598675

Centisome position: 45.52

GC content: 46.01

Gene sequence:

>726_bases
ATGCTAAGTAATATTCGTGTGGTTTTAGTGGGGACATCGCACCCCGGCAATATAGGTTCCACCGCCAGAGCGATGAAAAC
AATGGGTCTGTCTACGCTATATCTTGCCGAACCTCGCGTAGAACCCGATGGTCAATCTATTGCGCTTGCCGCGGGTGCTT
CTGATATTTTGAAACACCTCGTTAAAGTCGACTCACTCGCCGAAGCCATTGCCGATTGTAGTCTGGTCATTGCCACCAGT
GCGCGCAGCAGAACCTTAGATTGGCCTATGCTTGAACCACGCGAAGCAGGGCAGAAACTGGTTACAGAAAGCGTAACTGG
CCCTGTTGCCATTGTTTTTGGCCGTGAAAATCATGGGCTCAGCAATGAAGAGTTGCAACAATGTACTTATCATGTGGCGA
TCCCTGCAAATCCTGAGTACAGCTCACTTAACCTCGCTCAAGCGGTACAAATTATTTGTTATGAAACCCGCGTTGCACAC
TTAGCTGGAATTGAATCTGCACAAGAGCCGACAGAATATCCATTGGCAGCCGATCAAGAGCGTTTTTTTGTACACCTTGA
AAATACGTTATTTTCTACGGGCTTTATTATTAAAAACCACCCTGGACAAGTGATGACTAAATTGCGCCGTTTGTTCAGTC
GTGCACGGATTGAAAGCCAGGAAATGAATATTCTTAGAGGGATCCTGACTTCCATCGATAAAGTGGTTAGCAATAAACAA
AAATAG

Upstream 100 bases:

>100_bases
ATTTGATTGTTATATCAAATACCGATTTTCATATAAGCATTCGCCCGCGCCTGTGATAATATTCGCCCCCAGTGTTTTAT
GAATTAAATTAAGTTGTTTC

Downstream 100 bases:

>100_bases
TTAACCCTACAATAGTCAAAAATAATCGCGAAGGATAAACAATGGGCGTGATAGCTAGGCTAAAAGAAGATATTGAATCT
ATTTATCACCGTGATCCAGC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPRVEPDGQSIALAAGASDILKHLVKVDSLAEAIADCSLVIATS
ARSRTLDWPMLEPREAGQKLVTESVTGPVAIVFGRENHGLSNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAH
LAGIESAQEPTEYPLAADQERFFVHLENTLFSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVSNKQ
K

Sequences:

>Translated_241_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPRVEPDGQSIALAAGASDILKHLVKVDSLAEAIADCSLVIATS
ARSRTLDWPMLEPREAGQKLVTESVTGPVAIVFGRENHGLSNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAH
LAGIESAQEPTEYPLAADQERFFVHLENTLFSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVSNKQ
K
>Mature_241_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPRVEPDGQSIALAAGASDILKHLVKVDSLAEAIADCSLVIATS
ARSRTLDWPMLEPREAGQKLVTESVTGPVAIVFGRENHGLSNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAH
LAGIESAQEPTEYPLAADQERFFVHLENTLFSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVSNKQ
K

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=238, Percent_Identity=66.8067226890756, Blast_Score=320, Evalue=4e-89,
Organism=Escherichia coli, GI1790865, Length=179, Percent_Identity=35.7541899441341, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26349; Mature: 26349

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPRVEPDGQSIALAAGASDILKHL
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCHHHHHHHH
VKVDSLAEAIADCSLVIATSARSRTLDWPMLEPREAGQKLVTESVTGPVAIVFGRENHGL
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
SNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAHLAGIESAQEPTEYPLAADQE
CHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RFFVHLENTLFSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVSNKQ
EEEEEECCHHHHCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
K
C
>Mature Secondary Structure
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPRVEPDGQSIALAAGASDILKHL
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCHHHHHHHH
VKVDSLAEAIADCSLVIATSARSRTLDWPMLEPREAGQKLVTESVTGPVAIVFGRENHGL
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
SNEELQQCTYHVAIPANPEYSSLNLAQAVQIICYETRVAHLAGIESAQEPTEYPLAADQE
CHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RFFVHLENTLFSTGFIIKNHPGQVMTKLRRLFSRARIESQEMNILRGILTSIDKVVSNKQ
EEEEEECCHHHHCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA